89 research outputs found
Comparison of a Formulated Transport Medium and EDTA Anticoagulant in Kenyan Field Isolates from Maseno Division against a Panel of Antimalarials Using SYBR Green I technique
No full textIn vitro drug sensitivity relies on the growth of plasmodium in the presence of the antimalarials. These fresh isolates brought in for assay from collection sites normally not near the laboratory require a medium to keep the parasites viable in order to produce results which are a representation of the field situation in terms of drug susceptibility. This study validated the use of a formulated transport medium (TM) by assaying 322 Plasmodium falciparum field isolates from patients visiting Chulaimbo sub county hospital in Maseno division, for 50% inhibitory concentrations (IC50) against four antimalarials using SYBR Green 1 in vitro assay. These samples were transported using a formulated transport medium (TM) and the conventional EDTA anticoagulant tubes to act as control. The similarity which was detected with no significant difference (P >0.05) observed in the medians inhibitory concentration 50 (IC50) between samples in the EDTA anticoagulant and TM warrants its continued use. However, dihydroartemisinin (DHA) which showed to have a correlation significance (p<0.05) with most of the drugs used, calls for more research to expound on this finding
Liver biomarkers of silymarin milk thistle on paracetamol induced liver toxicity in adult albino rats (Rattus norvegicus)
No full textBackground: Paracetamol is the most common analgesic that is readily available with or without prescription over the counter and its toxicity is due to misuse or overdose associated with liver damage. Silymarin milk thistle is an edible herb believed to have antioxidant, scavenging and regulation of glutathione contents within the cell. Aim of the study was to determine the effects of different doses of silymarin milk thistle on liver biomarkers of paracetamol induced liver toxicity among albino rats.
Methods: Twenty-four adult albino rats were randomly grouped into four groups each group consisting of six animals. Paracetamol-only group given high dose paracetamol-750 mg/kbwt and control group received water and food only. The experimental group, were divided into three subgroups with all receiving high dose paracetamol for five days and thereafter given varying doses of silymarin milk thistle. SIL-G1 given 200 mg/kbwt of silymarin milk thistle, SIL-G2 given 400 mg/kbwt of silymarin milk thistle and SIL-G3 was given 600 mg/kbwt of silymarin milk thistle. All the animals were fed with standard rodent foods and water ad libitum. After completion of 24 hours of the last drug administration, they were humanely sacrificed, serum biochemical analysis of liver biomarkers (ALP, ALT and AST) was determined.
Results: High dose of paracetamol induced liver toxicity. Upon administration of high dose of silymarin milk thistle, there was restoration of ALP, AST and ALP of all these biomarkers in relation to the control group.
Conclusions: These findings showed that high dose (600 mg/kbwt) of silymarin milk thistle was found to have restorative effects and normalized liver biomarkers of ALP, AST and ALT
I minori con disabilità e gli strumenti giuridici per l\u27effettività del diritto all\u27inclusione scolastica
No full textIn this contribution the Author analyses the Italian legislation on school inclusion between constitutional profiles and social and organisational implications.In questo contributo l’Autore analizza, la disciplina italiana sull’inclusione scolastica tra profili costituzionali e implicazioni sociali ed organizzative
gli studi sul basso medioevo nella "Rivista di filosofia neo-scolastica" (1957-2008)
No full textIn the last 5o years on the Rivista di Filosofia neo-scolastica has been published several studies about XII-XIV centuries. Many are about theory of knowledge and metaphysics; a lot are focused on ethical problems; some on linguistic themes. Thomas Aquinas is the author more studied; but also franciscans such as William of Ockham and John Duns Scotus or Bonaventure recived attention
Per una storia della dirigenza scolastica. Maria Bartolini direttrice ed ispettrice scolastica del Novecento
No full textIl saggio mostra lo svolgersi della carriera professionale di Maria Bartolini, poliedrica e longeva figura di donna, scrittrice per l’infanzia, maestra, poi direttrice didattica e ispettrice scolastica che ha attraversato diverse stagioni scolastiche tra la fine dell’Ottocento, l’età giolittiana, il fascismo e l’Italia repubblicana. Uno studio di caso che s’inserisce pertanto all’interno della storia della professione docente e direttiva in Italia, tramite l'approccio microstorico.
The essay traces the professional career of Maria Bartolini, a multi-faceted woman who lived to a long age. Author of children’s literature, teacher, but also director of studies and school inspector, he career crossed different scholastic seasons running from the late 1800s, the Giolittian age, Fascism and Republican Italy. Consequently, this case is part of the history of teaching and school management profession in Italy
La lingua
No full textIn the context of the research-report published in the volume “Famiglie sotto esame. Una ricerca sull’immigrazione italiana in Germania e l’esperienza scolastica delle nuove generazioni” (co-edited by the Author), this chapter focuses the attention on the linguistic practices and behaviours adopted by the Italian families
Conclusioni. Eppur si muove... le strategie delle famiglie italiane
No full textIn the context of the research-report published in the volume “Famiglie sotto esame. Una ricerca sull’immigrazione italiana in Germania e l’esperienza scolastica delle nuove generazioni” (co-edited by the Author), this chapter focuses the attention on the strategies implemented by both the German institution and the Italian families and associations in order to sustain the school careers of Italian children
Comparison of a Formulated Transport Medium and EDTA Anticoagulant in Kenyan Field Isolates from Maseno Division Against a Panel of Antimalarials Using SYBR Green I Technique
No full textIntroduction: In vitro drug sensitivity relies on the growth of plasmodium in the presence of the antimalarials. These fresh isolates brought in for assay from collection sites normally not near the laboratory require a medium to keep the parasites viable in order to produce results which are a representation of the field situation in terms of drug susceptibility. Methods: This study validated the use of a formulated transport medium (TM) by assaying 322 Plasmodium falciparum field isolates from patients visiting Chulaimbo sub county hospital in Maseno division, for 50% inhibitory concentrations (IC50) against four antimalarials using SYBR Green 1 in vitro assay. These samples were transported using a formulated transport medium (TM) and the conventional EDTA anticoagulant tubes to act as control.Results: Similarity was detected with no significant difference (P >0.05) observed in the medians inhibitory concentration 50 (IC50) between samples in the EDTA anticoagulant and TM. However, dihydroartemisinin (DHA) which showed to have a correlation significance (p<0.05) with most of the drugs used, Conclusion: Variations in correlations between the pairs of drugs calls for more research to expound on this finding. Otherwise, similarity between the two anticoagulants its warrants continued use
Sensitivity of 3D7 and W2 Clones in a Formulated Transport Medium and EDTA Anticoagulants against a Panel of Antimalarials
No full textIntroduction: in vitro drug sensitivity relies on the growth of plasmodium in the presence of the antimalarials. These fresh isolates brought in for assay from collection sites normally not near the laboratory require a medium to keep the parasites viable in order to produce results which are a representation of the field situation in terms of drug susceptibility. Since factors such as mixed infection of drug resistant and sensitive parasites occurring in most field isolates can influence drug test outcome because they have different drug susceptibilities, Method: This study validated the use of a formulated transport medium (TM) by assaying clones which have been culture adapted in the lab for 50% inhibitory concentrations (IC50) against four antimalarials using SYBR Green 1 in vitro assay. These clones (3D7 and W2) were revived and recultured for assay. Prior to subjecting them to drugs, they were exposed to 2ml formulated TM and the conventional EDTA anticoagulant in 5ml vacutainer tubes to act as control.Results: Similarity was detected with no significant difference (P >0.05) observed in the medians inhibitory concentration 50 (IC50) between samples in the EDTA anticoagulant and TM holds however, samples in EDTA generally had higher IC50 values.Conclusion: This study concludes that the formulated TM holds promise for future use, Keywords: clones, transport medium, EDTA, susceptibility, inhibitory concentration 50, isolate
La scuola
No full textIn the context of the research-report published in the volume “Famiglie sotto esame. Una ricerca sull’immigrazione italiana in Germania e l’esperienza scolastica delle nuove generazioni” (co-edited by the Author), this chapter focuses the attention on the relationship between the Italian families and the German education system, analysing topics such as the attitudes of the teachers, the difficulties faced by Italian students (and particularly Italian students with a weak socio-cultural background), the existence of prejudices and discriminations, the behaviours of the Italian families
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