4,709 research outputs found
Radioactive reverse transcription PCR (RT-PCR) analysis of strain SSB318 complemented with wt or C238/C239
No full text<p><b>Copyright information:</b></p><p>Taken from " and investigation of bacterial type B RNase P interaction with tRNA 3′-CCA"</p><p></p><p>Nucleic Acids Research 2007;35(6):2060-2073.</p><p>Published online 13 Mar 2007</p><p>PMCID:PMC1874595.</p><p>© 2007 The Author(s)</p> PCR products were analyzed on a 10% polyacrylamide/8 M urea gel. Lanes 1–30: total RNA from SSB318 complemented with wt (lanes 1–4 and 13-16), C238 (lanes 5–8, 17–20 and 25–30) or C239 (lanes 9–12 and 21–24) grown at 37°C in the absence of IPTG and in the presence of 2% xylose (w/v); amounts of total RNA were 200 ng in lanes 1–24, 26 and 29, 100 ng in lanes 25 and 28, and 400 ng in lanes 27 and 30. P : presence (+) or absence (−) of a xylose-inducible plasmid-encoded gene. Lanes 1–12 and 25–27: primers specific for ; lanes 13–24 and 28–30: primers specific for the mRNA encoding ribosomal protein S18 (S18). AMV: presence (+) or absence (−) of reverse transcriptase. For details on RT-PCR, see the Material and Methods section. Lanes 25–30 document that the amount of RT-PCR product was sensitive to RNA template concentration. The figure illustrates a representative experiment, but the results shown here were reproduced in five individual experiments using three independent total RNA preparations
Prescribing by mental health nurses: the UK perspective
No full textPURPOSE. This article aims to discuss the growth of mental health nurse (MHN) prescribing in the United Kingdom as an exemplar for readers to compare progress in their own countries and context. This study also aims to provide a historical overview of this process in the United Kingdom where MHNs prescribe safely and competently.
CONCLUSIONS. Finally, evidence has shown that MHNs with prescriptive authority are competent when prescribing when compared to psychiatrists.
PRACTICE IMPLICATIONS. Despite organizational barriers and educational concerns, MHN prescribing is becoming embedded in the healthcare context in the United Kingdo
Modular RT-Motion USB Software Framework
No full textPhilips Applied Technologies has developed the RT-Motion USB platform as a compact distributed real-time motion control platform, but the platform can still be improved by developing a more advanced software framework. The goal of this thesis project is to design a modular software framework to complement the RT-Motion USB platform with extendability, flexibility, and configurability. The design focuses on the extendability of the platform by developing foundation building blocks to integrate software extension modules and device drivers easily. The design emphasizes the principle of simplicity to ensure the lowest possible overhead and highest reliability. The firmware is modular, which allows each module to concentrate on its own area. The implementation of the design has been tested and is proved to provide extendability, flexibility, and configurability while incurring low overhead. The improvement to the RT-Motion USB platform is expected to extend the applicability of the RT-Motion USB platform to a broader application range.Microelectronics & Computer EngineeringElectrical Engineering, Mathematics and Computer Scienc
Exploring the effects od a rigid body on the evolution of the Rayleigh-Taylor instability
No full textThis talk discusses the effects of a rigid solid boundary impeding the evolution of the Rayleigh-Taylor (RT) instability. The introduction of an obstacle completely alters the evolution of RT growth, instead of mixing the domain rapidly, a quasi-steady flow, rich in dynamics is established for long periods of time. Using a combination of low Atwood number experiments and ILES simulations, this talk will present a non-dimensional analytical model for a multi-stage mixing process, discussing the effects of the opening size and topology on the density change of each layer, buoyancy driven flux through the opening and mixing efficiency
Small-Scale Properties of Two-Dimensional Rayleigh-Taylor Turbulence
No full textWe report a high-resolution numerical study of small-scale properties of two-dimensional (2D) miscible Rayleigh-Taylor (RT) incompressible turbulence with the Boussinesq approximation at small Atwood number and unit Prandtl number. Our results show that the buoyancy force balances the inertial force at all scales below the integral length scale and thus validate the basic force-balance assumption of the Bolgiano-Obukhov scenario in 2D RT turbulence. We further examine other small-scale properties of 2D RT turbulence, such as temporal evolution of energy and thermal dissipation rates, the emergence of intermittency and anomalous scaling for high order moments of velocity and temperature differences, distributions of local dissipation scales, and so on
ナイジェリア南東部および中南部におけるラッサウイルス検出のためのRT-LAMPアッセイの開発
No full textLassa virus (LASV) causes Lassa fever (LF), a viral hemorrhagic fever endemic in West Africa. LASV strains are clustered into six lineages according to their geographic location. To confirm a diagnosis of LF, a laboratory test is required. Here, a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay using a portable device for the detection of LASV in southeast and south-central Nigeria using three primer sets specific for strains clustered in lineage II was developed. The assay detected in vitro transcribed LASV RNAs within 23 min and was further evaluated for detection in 73 plasma collected from suspected LF patients admitted into two health settings in southern Nigeria. The clinical evaluation using the conventional RT-PCR as the reference test revealed a sensitivity of 50% in general with 100% for samples with a viral titer of 9500 genome equivalent copies (geq)/mL and higher. The detection limit was estimated to be 4214 geq/mL. The assay showed 98% specificity with no cross-reactivity to other viruses which cause similar symptoms. These results suggest that this RT-LAMP assay is a useful molecular diagnostic test for LF during the acute phase, contributing to early patient management, while using a convenient device for field deployment and in resource-poor settings.長崎大学学位論文 学位記番号:博(医歯薬)甲第1265号 学位授与年月日:令和2年9月18日Author: Christelle M. Pemba, Yohei Kurosaki, Rokusuke Yoshikawa, Olamide K. Oloniniyi, Shuzo Urata, Maki Sueyoshi, Vahid R. Zadeh, Ifeanyi Nwafor, Michael O. Iroezindu, Nnenna A. Ajayi, Chinedu M. Chukwubike, Nneka M. Chika-Igwenyi, Anne C. Ndu, Damian U. Nwidi, Yuki Maehira, Uche S. Unigwe, Chiedozie K. Ojide, Emeka O. Onwe, Jiro YasudaCitation: Journal of Virological Methods, 269, pp.30-37; 201
The relationship between co-authorship, currency of references and author self-citations
No full textThis paper attempts to identify the relationship between co-authorship and the currency of the references and author self-citations in the key journals of environmental engineering. The results show that the self-citation rate of co-authored articles is higher than in single-authored articles. A statistically significant correlation is identified between the numbers of co-authors, the rate of author self-citing and the author self-cited; though it was a low correlation. The value of coefficient correlation between the number of co-authors and the author self-citing rate is slightly higher than that between the number of co-authors and the author self-cited rate, which indicates that the number of co-authors hold a stronger correlation with the self-citing rate than the self-cited rate. Meanwhile, self-citing references are found to be more up-to-date than references to others. The range of publication years of self-citing references is smaller than that of references to others, indicating that researchers tend to preferentially cite their own recent works. There is no significant difference in the latest references between self-citing references and the references to others. It might result from electronic journals that provide an easy access to the most current publications.補正完畢國外SSCIY紙本電子版HU
Expression of Plasmodium falciparum genes involved in erythrocyte invasion varies among isolates cultured directly from patients.
No full textPlasmodium falciparum merozoites invade erythrocytes using a range of alternative ligands that includes erythrocyte binding antigenic proteins (EBAs) and reticulocyte binding protein homologues (Rh). Variation in the expression of some of these genes among culture-adapted parasite lines correlates with the use of different erythrocyte receptors. Here, expression profiles of four Rh genes and eba175 are analysed in a sample of 42 isolates cultured from malaria patients in Kenya. The profiles cluster into distinct groups, largely because of very strong negative correlations between the levels of expression of particular gene pairs (Rh1 versus Rh2b, eba175 versus Rh2b, and eba175 versus Rh4), previously associated with alternative invasion pathways in culture-adapted parasite lines. High levels of eba175 are seen in isolates in expression profile group I, and may be associated with sialic acid-dependent invasion. Groups II and III are, respectively, characterized by high levels of Rh2b and Rh4, and are more likely to be associated with sialic acid-independent invasion
RT-176 MP models
No full textProvided to Systems Engineering Research Center (SERC) by the author as part of a list of publications. Relates to Research Task RT-176, Start Date: 2018-11-07; End Date: 2018-11-07. The article of record as published may be found at https://sercuarc.org/researcher/?id=184
Development and optimisation of a duplex real-time reverse transcription quantitative PCR assay targeting the VP7 and NS2 genes of African horse sickness virus
No full textNucleotide sequences of 52 South African isolates of African horse sickness virus (AHSV) collected during 2004–2005 and including viruses of all nine AHSV serotypes, were used to design and develop a duplex real-time reverse transcription quantitative PCR (RT-PCR) assay targeting the VP7 (S8) and NS2 (S9) genes of AHSV. The assay was optimized for detection of AHSV in fresh and frozen blood of naturally infected horses. Assay performance was enhanced using random hexamers rather than gene-specific primers for RT, and with denaturation of double-stranded RNA in the presence of random hexamers. The assay was efficient with a linear range of at least five orders of magnitude. The analytical sensitivity of the assay was 132 copies of the target genes (4125 copies per ml of blood), and the assay was at least 10-fold more sensitive than virus isolation on BHK-21 cells. The assay was also highly specific because it did not detect related orbiviruses, such as bluetongue and equine encephalosis viruses.ID: S0166093410000893; M3: Article; Accession Number: S0166093410000893; Author: M. Quan (a, b, ⁎); Author: C.W. Lourens (a, b); Author: N.J. MacLachlan (c); Author: I.A. Gardner (d); Author: A.J. Guthrie (a); Affiliation: Equine Research Centre, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa; Affiliation: Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa; Affiliation: Equine Viral Disease Laboratory, Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA; Affiliation: Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA; Keyword: African horse sickness virus; Keyword: Real-time quantitative RT-PCR; Keyword: VP7 gene; Keyword: NS2 gene; Keyword: Duplex; Number of Pages: 8; Language: English
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