5,115 research outputs found
Structural and functional characterization of S-adenosylmethionine (SAM) synthetase from Pichia ciferrii
No full textS-adenosylmethionine synthetase (SAM-s) catalyzes the synthesis of S-adenosylmethionine (SAM), which is essential for methylation, transcription, proliferation, and production of secondary metabolites. Here SAM-s from Pichia ciferrii were selectively cloned using RNA CapFishing and rapid amplification of cDNA ends (RACE). The putative full-length cDNA of SAM-s encoded a 383 amino acid protein (42.6 kDa), which has highly conserved metal binding sites, a phosphate-binding site, and functionally important motifs. The corresponding enzyme was over-expressed in a heterologous host of Pichia pastoris, and then purified to a homogenous form. Enzyme kinetics, immunoblotting, circular dichroism (CD), high performance liquid chromatography (HPLC), and molecular modeling were conducted to characterize the SAM-s from P. ciferrii. Structural and functional studies of SAM-s will provide important insights for industrial applications.open
Chinese literary works translated into Baba Malay: a bibliographical study
No full textAnalyses 68 unique titles of Baba translated works published between 1889 and 1950. The titles are held in the libraries of the University of Malaya (UM), Science University Malaysia (USM), National University of Malaysia (UKM), the Dewan Bahasa dan Pustaka (DBP), National University of Singapore (NUS), National Library of Singapore (NLS) and the British Library (BL). The results reveal three periods of active publication of Baba translated works. A total of 18 works were translated before World War I, followed by 10 just after the war, 39 titles were published before the break of the World War II and 1 was identified in 1950. There were 103 persons involved in the 68 translated works, some of whom are responsible for more than one title. The most prominent translators were Chan Kim Boon, Wan Boon Seng, Seow Chin San and Lee Seng Poh. Some of the translators were also be editors, illustrators or editors. There were 31 publishers and 21 printing presses involved, all were located in Singapore. The most active publishers were Wan Boon Seng, Kim Seck Chy Press and Nanyang Romanised Malay Book Co. The translated works mainly cover historical classical Chinese stories, chivalrous stories, romances, folklore and legends. The titles were priced between 10 cents to 2 dollars in Straits currency. The University of Malaya Library held the largest number of unique title (62) out of which 15 were unique titles
Dr. Glendon Swarthout
No full textHosted by Roger M. Busfield, MSU Assistant Professor of Speech and Theater, Meet the Author is designed to introduce a general audience to a contemporary author and their work through in-depth interviews. This episode features a conversation between Dr. Glendon Swarthout, prolific author and English professor at MSU, and assistant professors Sam S. Baskett and Theodore B. Strandness
How to Explain Behavior: Author Precis
No full textThis is an author precis of the book How to Explain Behavior: A Critical Review and a New Approach by Sam S. Rakover (2018, Lexington). The precis has two sections. The first, basic methodological origin-points, treats the fundamental ideas and premises concerning explanation and understanding. The second section outlines the book's arrangement, and summarizes the content of each chapter
Advances in Robotics, Proceedings of the FIRA RoboWorld Congress 2009
No full textKim J-H, Sam Ge S, Vadakkepat P, et al. Advances in Robotics, Proceedings of the FIRA RoboWorld Congress 2009. Lecture Notes in Computer Science. Vol 5744 1st ed. Incheon, Korea: Springer; 2009
Tools for interactive multiattribute decisionmaking with incompletely identified information
No full textThe aim of this paper is to present tools or techniques for an interactive multiattribute decisionmaking with the decision maker's incomplete information. That is: (1) We formally describe mathematical models under certainty and uncertainty for establishing pairwise dominance, where the incomplete information becomes the set of constraints in the model. (2) We present a description on styles of incomplete parameter value information. (3) The characteristic of a weak dominance is described. When the decision maker is not willing or able to provide more information on parameters, the weak dominance technique is useful for a final choicemaking. (4) We propose a dominance graph and also present an algorithm of generating the dominance graph based on the information of pairwise dominance. The dominance graph compactly displays the dominance structure of all alternatives, thus it can be used for aiding the selection of preferable alternatives. (C) 1997 Published by Elsevier Science B.V
The importance of examining movements within the US health care system: sequential logit modeling
No full textAbstract Background Utilization of specialty care may not be a discrete, isolated behavior but rather, a behavior of sequential movements within the health care system. Although patients may often visit their primary care physician and receive a referral before utilizing specialty care, prior studies have underestimated the importance of accounting for these sequential movements. Methods The sample included 6,772 adults aged 18 years and older who participated in the 2001 Survey on Disparities in Quality of Care, sponsored by the Commonwealth Fund. A sequential logit model was used to account for movement in all stages of utilization: use of any health services (i.e., first stage), having a perceived need for specialty care (i.e., second stage), and utilization of specialty care (i.e., third stage). In the sequential logit model, all stages are nested within the previous stage. Results Gender, race/ethnicity, education and poor health had significant explanatory effects with regard to use of any health services and having a perceived need for specialty care, however racial/ethnic, gender, and educational disparities were not present in utilization of specialty care. After controlling for use of any health services and having a perceived need for specialty care, inability to pay for specialty care via income (AOR = 1.334, CI = 1.10 to 1.62) or health insurance (unstable insurance: AOR = 0.26, CI = 0.14 to 0.48; no insurance: AOR = 0.12, CI = 0.07 to 0.20) were significant barriers to utilization of specialty care. Conclusions Use of a sequential logit model to examine utilization of specialty care resulted in a detailed representation of utilization behaviors and patient characteristics that impact these behaviors at all stages within the health care system. After controlling for sequential movements within the health care system, the biggest barrier to utilizing specialty care is the inability to pay, while racial, gender, and educational disparities diminish to non-significance. Findings from this study represent how Americans use the health care system and more precisely reveals the disparities and inequalities in the U.S. health care system.</p
An appeal to California
No full textAn article written by Pearl S. Buck regarding California's place in American and international politics as well as the idea of whiteness in the United States and globally. The article was taken from an address given at a Town Hall luncheon in Los Angeles. There are handwritten annotations on the article in Japanese.The Chuman (Hayao "Sam" and Toshiko) Papers documents the World War II experiences of Hayao "Sam" and Toshiko Chuman, who were Kibei Nisei born in the United States but grew up and completed school in Japan, and then returned to the U.S. prior to the war. It chronicles the Chuman's incarceration from the Santa Anita Assembly Center, through Jerome, Rohwer, Tule Lake camps, and the Santa Fe and Crystal City internment camps as well as their struggle for restoring their U.S. citizenships in the 1960s. The digital collection consists of mostly textual material, including correspondence, affidavits, incarceration camp records, lease agreements, financial documents, receipts, pamphlets, and booklets
Physical dance performance: An investigation into the development of a performance technique based on the integration of certain Korean dance technique and contemporary Western styles of dance and physical theatre
No full textThis thesis was submitted for the degree of Doctor of Philosophy and awarded by Brunel University, 04/10/2001.This development of a performer practice that integrates elements of traditional Korean dance technique and Western forms of physical theatre and contemporary dance is based on an approach to internal understanding and external execution. Central to the work is the concept of body energy, or Ki. This ancient Eastern term is translated into a contemporary practice that enables a performer to engage mental and physical training. Breath and the use of breath in performing are the principal means of achieving this level of engagement
Progress in Robotics, Proceedings of the FIRA RoboWorld Congress 2009
No full textKim J-H, Sam Ge S, Vadakkepat P, et al. Progress in Robotics, Proceedings of the FIRA RoboWorld Congress 2009. Lecture Notes in Computer Science. Vol. 44, 2009, ISBN: 978-3-642-03985-0. Incheon, Korea: Springer; 2009
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