7,509 research outputs found
Automated model structure variation and policy robustness testing: A procedure for innovation diffusion models
No full textn this paper we present a procedure that enables a systematic and partially automated parameterization and selection of agent-based models of the diffusion of innovations and policies to support diffusion processes. A prototype on the diffusion of water saving shower heads is presented. Our results suggest that the presented approach can adequately and systematically evaluate different sets of parameters and model structures against known diffusion data.Energie and Industri
Author Checklist - Full - Ahmed Essa_ 135540.pdf
No full textAttached is a required author checklist for animal research </p
Functional Adaptation of Escherichia coli Inducible Promoter Systems for Use in Pseudomonas putida and Bacillus subtilis
No full textMennesker har brukt mikrober i tusenvis av år, og gjør det nå i større grad enn noen gang før. Anvendelsesområdene er mangfoldige, fra småskala fermentering under hjemmebrygging til storskala industriell biosyntese av farmasøytiske forbindelser. De tidligste anvendelsene kom imidlertid fra tilfeldige oppdagelser som var få og spredte. Syntetisk biologi som disiplin har åpnet veien for hurtig utvikling av mikrobiel ingeniørkunst for spesifikke nye formål, som produksjon av humant insulin gjennom tarmbakterien, Escherichia coli (E. coli). Genetisk forskning har fått et banebrytende verktøy i syntetisk biologi, som muliggjør uttrykk av gener på tvers av flere mikrobielle arter. Induserbare genuttrykksystemer er et godt eksempel. Disse systemene gir oss mulighet til å kontrollere uttrykket av et gen av interesse i vertsmirober, ved å justere konsentrasjonen av et lite indusermolekyl i kulturmediet.
I denne oppgaven undersøker jeg tilpasningsgraden til velkarakteriserte E. coli indusersystemer for bruk i nye verter, Pseudomonas putida (P. putida) og Bacillus subtilis (B. subtilis). Adapsjon ble gjort ved å introdusere nye vertsoptimaliserte promotorer i stedet for de iboende induserbare systempromotorene. To promotorer i hvert system var mål for optimalisering. En av promotorene styrer uttrykket av den induserbare Transkripsjonsfaktor (TF). TFen regulerer transkripsjonen fra den induserbare promotoren, som styrer uttrykket av reportergenet. Disse systemene induceres av følgende seks indusere: Vanillinsyre (Van), Isopropyl β-D-1-tiogalaktopyranosid (IPTG), 4-isopropylbenzosyre (Cuma), 3,4-Dihydroksybenzosyre (DHBA), Anhydrotetrasyklin hydroklorid (aTc) og l-Arabinose (Ara). Nye promotorer ble forhåndsgenerert ved hjelp av en prediktiv Transformer-basert kunstig intelligens. Resultatene viser varierende nivåer av suksess.
Adaptasjonen for P. putida resulterte i økte induksjonsnivåer for aTc-systemet da det ble dyrket i 0,2 μM aTc, noe som ikke ble overført til 2,0 μM. Da jeg sammenlignet med data fra E. coli generert for denne oppgaven, viste IPTG- og Ara-systemene direkte kompatibilitet med P. putida uten optimalisering. Adaptasjon ga ikke forhøyet induksjon. Van-, Cuma- og DHBA-systemene viste ikke direkte kompatibilitet, og adaptasjon indikerte ikke forhøyet induksjon i P. putida.
Cuma-systemet var det eneste systemet jeg testet for kompatibilitet med B. subtilis. Heterolog yfp ble testet for funksjonalitet på tvers av arter ved å først klone et B. subtilis gfp-derivat av Cuma-systemet. Jeg utviklet konstitutivt aktive P43-derivater av disse induserbare systemene for det samme formålet. Mutasjoner i reportergene og fravær av en nødvendig tilleggssekvens for promotoren, reduserte sannsynligvis fluorescensnivåene ned til de negative kontrollene. Jeg klonete et konstitutivt aktivt derivat med klonet yfp under kontroll av Pveg. Dette systemet utøvde ikke høyere fluorescens enn negativ kontroll. Dette tyder på at heterolog yfp ikke kan benyttes som reporter i B. subtilis. Jeg foreslår å klone et homologt B. subtilis reportergen inn i det samme Pveg-systemet for å innhente ytterligere bevis for disse funnene.Humans have been utilising microbes for thousands of years and do so now more than ever. The applications are multifaceted, ranging from small-scale fermentation during homebrewing to the large-scale industrial biosynthesis of pharmaceutical compounds. Historically, many early applications resulted from accidental discoveries, occurring infrequently. The discipline of synthetic biology has opened up the path of fast-paced microbial engineering for specific and novel purposes, such as the production of human insulin in the gut microbe, Escherichia coli (E. coli). Genetic research has acquired a fundamental tool in synthetic biology, enabling the expression of genes across multiple microbial species. Inducible gene expression systems are a profound example, empowering us to control the expression of a gene of interest in host microbes by adjusting the concentration of a small inducer molecule in the culture medium.
In this thesis, I investigate the adaptability of well-characterised E. coli inducer systems for use in new hosts, Pseudomonas putida (P. putida) and Bacillus subtilis (B. subtilis). Adaptation was done by introducing novel host-optimised promoters in place of native inducible systems promoters. The two promoters in each system were targets for optimisation. One promoter directs expression of the inducer sensor Transcription Factor (TF). The TF regulates transcription from the inducible promoter, which directs the expression of the reporter gene. These systems were targeted by the following six inducers: Vanillic acid (Van), Isopropyl β-d-1-thiogalactopyranoside (IPTG), Cuminic acid (Cuma), 3,4-Dihydroxybenzoic acid (DHBA), Anhydrotetracycline hydrochloride (aTc) and l-Arabinose (Ara). Novel promoters were generated beforehand via the predictive power of a Transformer-based artificial intelligence deep learning architecture. The adaptation efforts and induction results demonstrate varying levels of success.
Adaptation efforts for P. putida only resulted in increased induction outputs for the aTc-system when grown in 0.2 μM aTc, which did not transfer over to the 10-fold aTc concentration. When compared to E. coli data generated for this thesis, I found the IPTG- and Ara-systems directly compatible with P. putida without any optimisation. Adaptation efforts did not improve this pattern. The Van-, Cuma- and DHBA-systems did not exhibit compatibility “out-of-the-box” and adaptation efforts did not indicate improved induction in P. putida.
The Cuma-system was the only system tested for compatibility with B. subtilis. Specifically, the heterologous yfp had its cross-species functionality tested, by first cloning a B. subtilis gfp Cuma-system derivative. I developed constitutively active P43-derivatives of these inducible systems for the same purpose. Mutations in the reporter coding sequences and the absence of a likely critical auxiliary promoter sequence produced inconclusive compatibility results. I cloned a constitutively active Pveg-YFP-derivative, which did not produce fluorescence relative to negative control. This partly indicates the incompatibility of heterologous yfp in B. subtilis. I suggest cloning a homologous B. subtilis positive control system to generate stronger evidence of these findings
Response to correspondence from the ESSA Statement authors
No full textResponse to correspondence from the ESSA Statement author
Response to correspondence from the ESSA Statement authors
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Characterizing seasonal residential water use in the Khan Younis Governorate using Landsat 8 – Derived land surface temperature
No full textUrbanization increases land surface temperature (LST) due to urban surfaces radiating heat, which is influenced by variations in building materials, heights and spacing, and street geometry amongst other natural and manmade factors [1]. LST increases water consumption especially in summer, due to human behavior such as increased showering, drinking, entertainment and cooling. This research aims to use thermal remote sensing data and GIS tools to quantify the impact of LST on water consumption (WC) for the different seasons in Khan Younis Governorate, Palestine. Correlation was studied between water meter data for 2017 (28,000 houses) and the corresponding LST data of Landsat 8 at 100 m resolution. This study shows the potential of remotely sensed LST datasets for estimating WC under various seasonal climatic conditions, which has the potential to be a valuable tool for the management of water resources in urban areas
Modification data for the end of term American Foreign Policy essay
No full textDataset and do file used for the end of term essa
Connections between Author and Narrator-protagonist: Autofiction in Essa gente, by Chico Buarque de Holanda
No full textO presente artigo propõe uma análise do romance Essa gente (2019), de Chico Buarque de Holanda, visando examinar aspectos da obra que evidenciam intersecções entre a vida do escritor e a história narrada. Parte-se do pressuposto de que o romance, escrito em primeira pessoa pelo protagonista, se situa no limiar entre autobiografia e ficção, no tipo de narrativa conhecido como autoficção, muito difundido atualmente. O hibridismo desse tipo de escrita de si convida o leitor a um constante questionamento sobre o que pertence à vida do autor e o que é construção ficcional. O aporte teórico para a discussão sobre a natureza da narrativa autoficcional será retirado dos estudos de Leyla Perrone-Moisés (2016), Karl Erik Schollhammer (2009), Serge Doubrovsky (2014), Luciene Azevedo (2008), Diana Klinger (2006) e Anna Faedrich (2015). O autor constrói um texto ambíguo e irônico no qual elementos autobiográficos e fictícios se entrelaçam possibilitando uma reflexão profunda sobre conflitos afetivos, declínios sociais, cicatrizes históricas e sobre a turbulenta conjuntura política brasileira de 2016 a 2019.
PALAVRAS-CHAVE: Essa gente; Chico Buarque; autoficção.This article offers an analysis of the novel Essa Gente (2019) by Chico Buarque de Holanda and seeks to examine aspects of the narrative that show possible intersections between the author’s life and the story told. The assumption is that the novel, told in the first person by the main character, stands on the threshold between autobiography and fiction, falling into the category known as autofiction, a form of narrative quite popular nowadays. The hybridity of this type of self-writing invites the reader to a constant questioning about what belongs to the author’s life and what is fictional creation. The theoretical support for the discussion on the nature of the autofictional text will be taken from the work of Leyla Perrone-Moisés (2016), Karl Erik Schollhammer (2009), Serge Doubrovsky (2014), Luciene Azevedo (2008), Diana Klinger (2006), and Anna Faedrich (2015). The author creates an ambiguous and ironic story in which autobiographical and fictional elements intertwine, promoting a deep reflection on affective conflicts, social declines, historical scars, and on the turbulent Brazilian political situation from 2016 to 2019.
KEYWORDS: Essa gente; Chico Buarque; autofiction
USO DA PLATAFORMA ONLINE KHAN ACADEMY NO PROCESSO DE ENSINO DA MATEMÁTICA BÁSICA: Percepções sobre as possibilidades de aprendizagem dos estudantes do EJA no contexto do IFC, Campus Camboriú
No full textO objetivo deste estudo é avaliar como a plataforma Khan Academy pode contribuir para o aprendizado de matemática entre os estudantes da Educação de Jovens e Adultos (EJA), servindo como um recurso complementar. O foco da pesquisa é investigar tanto a influência do uso da Khan Academy no desempenho acadêmico quanto na percepção dos estudantes da EJA em relação a essa ferramenta educacional. Espera-se que os resultados demonstrem a efetividade da Khan Academy em promover um ambiente de aprendizado mais dinâmico e acessível, além de fornecer insights sobre as necessidades específicas dos alunos da EJA
Evaluation of the Khan Academy platform as an auxiliary tool to learning process of Mathematics in elementary school.
No full textEste trabalho tem por objetivo estudar os benefícios que a plataforma digital de ensino Khan Academy pode proporcionar na aprendizagem de conceitos matemáticos aos alunos do ensino fundamental anos finais, com o propósito de resolver a seguinte questão: Existe diferença de aprendizagem entre os alunos que usam a plataforma Khan Academy como ferramenta auxiliar no estudo de conceitos aprendidos em sala de aula em comparação aos alunos que não a utilizam? Essa pesquisa foi aplicada em uma experiência de ensino online numa escola da rede particular na cidade de São Paulo com 64 alunos de 6° e 61 alunos de 7° anos. Antes e após a realização das atividades recomendadas na plataforma de ensino Khan Academy, os alunos foram submetidos a uma avaliação, gerando assim cerca de 1300 avaliações que foram analisadas seguindo conceitos estatísticos. Este trabalho teve uma abordagem qualitativa e quantitativa que, juntas, forneceram resultados de todo processo.The objective of this dissertation is to study the benefits that the digital teaching platform Khan Academy can provide for the learning of Mathematical concepts to students from elementary school to the final years with the purpose of solving the following question: Is there a difference in learning between the elementary years students who use the Khan Academy platform as an auxiliary tool in the study of concepts taught in the classroom and to students who do not use it? This research was applied in a private school online teaching experience in São Paulo City with 125 students whose age range is 10-12 years old. Before and after carrying out recommended activities on the Khan Academy teaching platform, the students were evaluated, which generated 1300 assessments that were analyzed following statistical criteria. This project had a qualitative and quantitative approach that, together, provided results of the entire process
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