13 research outputs found
Average Crack Spacing of Overlay-Strengthened RC Beams
No full textThe research is focused on evaluating the average crack spacing of overlay-strengthened reinforced concrete (RC) beams. The current equations in different structural codes for predicting the average crack spacing of RC beam with multilayered-reinforcing bars is proved to be inapplicable for overlay-strengthened RC beam, although these two kinds of beams are both with reinforcement in multi-layers. Therefore, a simple, practical and new analytical model that is capable of predicting the average crack spacing of overlay-strengthened beams is developed. An overlay-strengthened beam element is analyzed based on equilibrium and compatibility equations to formulate the average stresses of concrete, overlay and tension reinforcement. A series of verification comparisons with the experimental investigations, which involved various types of beam elements, are performed to verify the validity and reliability of the model. The proposed model performs satisfactorily with respect to measured response from the experimental database
Battery resistance and its effects on performance of laminate film-type Co-PBA/Ni-PBA tertiary battery
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Estimation of Collection Efficiency Change of Moving Granular Bed Filter by Dust Load
No full textFinal goal of this study is to theoretically describe effects of influencing factors on the performance of the coke bed filter as a material classifier and to predict its optimal operating condition. In this paper, the author focuses on the basic investigation on collection mechanism of a fixed coke bed filter on the basis of the depth filtration theory, in which an influence of dust load on the dust collection efficiency
HOXD3-overexpression increases integrin alphavbeta3 expression and deprives E-cadherin while it enhances cell motility in A549 cells.
No full textWe have previously shown that transduction of HOXD3, one of homeobox genes, into human lung cancer A549 cells enhances cell motility, invasion and metastasis. In the present study, we examined the roles of integrin β3 which was up-regulated by HOXD3-overexpression in the HOXD3-induced motility of A549 cells. We first established integrin β3-transfectants and compared their motile activity to those of the HOXD3-transfected, control-transfected and parental cells by three different assays. The integrin β3-transfectants as well as the HOXD3-transfectants formed heterodimer with integrin αv subunit, and showed highly motile activities assessed by haptotaxis or phagokinetic track assay compared to the control transfectants or parental cells. In vitro wound-healing assay revealed that migratory activities were graded as the HOXD3-transfectants > the integrin β3-transfectants > the control transfectants or parental cells. E-cadherin was expressed in the integrin β3-transfectants but not expressed in the HOXD3-transfectants. An addition of function-blocking antibody to E-cadherin into the wound-healing assay promoted the migratory activity of the integrin β3-transfectants, suggesting that E-cadherin prevented the cells from dissociating from the wound edges. These results indicate that increased expression of integrin αv β3 and loss of E-cadherin by HOXD3-overexpression are responsible for the enhanced motility and dissociation
Gelsolin: a candidate for suppressor of human bladder cancer
No full textHuman transitional cell carcinomas of the bladder frequently reveal chromosomal abnormalities that span a range between chromosome 9pl2 and 9qter, even at early stages of bladder carcinogenesis. Because the gene that encodes an actin-regulatory protein, gelsolin, is localized in chromosome 9q33, we examined the expression of gelsolin in a number of human bladder cancer cell lines and tissues. In all 6 cell lines and in 14 of the 18 tumor tissues (77.8%), gelsolin expression was undetectable or extremely low in comparison with its expression in normal bladder epithelial cells. Furthemore, upon the introduction of the exogenous human or mouse authentic gelsolin cDNA into a human bladder cancer cell line, UMUC-2, gelsolin transfectants of UMUC-2 greatly reduced the colony-forming ability and the tumorigenicity in vivo. These results suggest that gelsolin plays a key role as a tumor suppressor in human urinary bladder carcinogenesis
Health-related quality of life associates with clinical parameters in patients with NTM pulmonary disease
No full textNagasaki University (長崎大学)博士(医学)BACKGROUND: Previous studies have shown a reduction in health-related quality of life (HRQoL) in patients with non-tuberculous mycobacterial pulmonary disease (NTM-PD). However, the causes of this decline and the factors that contribute to it are unknown. This study was conducted to analyse the association between the St George´s Respiratory Questionnaire (SGRQ) and clinical parameters, including age, disease duration, body composition, pulmonary function, chest X-ray findings, blood data and physical function. METHODS: We performed a single-centre, cross-sectional, retrospective study of 101 patients with NTM-PD from December 2016 to October 2019. The relationship between the SGRQ scores and clinical parameters was evaluated. RESULTS: The median patient age was 67.0 years. Pulmonary function, radiological score, albumin levels, C-reactive protein levels and incremental shuttle walk test distance (ISWD) were significantly correlated with the total and component scores on the SGRQ. Multiple regression analysis showed that the SGRQ score was significantly associated with radiological score, pulmonary function and ISWD. CONCLUSION: This study was the first to assess the effect of clinical parameters on the SGRQ in patients with NTM-PD. HRQoL as determined using the SGRQ was associated with the radiological score, pulmonary function and ISWD in patients with NTM-PD.長崎大学学位論文 [学位記番号]博（医歯薬）甲第1574号 [学位授与年月日]令和6年3月19日Author: Kawahara, K.; Tabusadani, M.; Yamane, K.; Takao, S.; Kuroyama, Y.; Matsumura, Y.; Mori, K.; Ono, K.; Omatsu, S.; Furuuchi, K.; Fujiwara, K.; Morimoto, K.; Kimura, H.; Senjyu, H.Citation: The International Journal of Tuberculosis and Lung Disease, 25(4), pp.299-304; 2021Nagasaki University (長崎大学), 博士(医学) (2024-03-19)doctoral thesi
Prevalence of and risk factors for depressive symptoms in non-tuberculous mycobacterial pulmonary disease
No full textNagasaki University (長崎大学)博士(医学)BACKGROUND: The presence of depressive symptoms in patients with non-tuberculous mycobacterial pulmonary disease (NTM-PD) is an important research topic; however, the prevalence of depressive symptoms and the factors that influence their development are unclear.
OBJECTIVE: To analyse the association between CES-D (Center for Epidemiological Studies Depression Scale) scores and clinical parameters such as age, disease duration, pulmonary function, imaging findings, blood data, physical functions, sleep disturbances, respiratory symptoms and health-related quality of life (HRQOL).
METHODS: We conducted a cross-sectional retrospective study of 114 patients with NTM-PD at a single centre from March 2016 to January 2021 to evaluate the relationship between CES-D scores and clinical parameters.
RESULTS: Participants had a median age of 64 years; 32.5% of them had depressive symptoms. Disease duration, albumin, C-reactive protein, pulmonary function, dyspnoea, exercise capacity, respiratory symptoms, cough-related HRQOL and sleep disturbances were associated with depressive symptoms. Binomial logistic regression analyses indicated that the CES-D score was significantly associated with cough-related HRQOL and sleep disturbances.
CONCLUSION: A high percentage of NTM-PD patients in this study experienced depressive symptoms, and these patients had abnormalities of various clinical parameters. Cough-related HRQOL and sleep disturbance had a strong influence on the development of depressive symptoms.長崎大学学位論文 学位記番号:博(医歯薬)甲第1490号 学位授与年月日:令和5年3月20日Author: Matsumura, Y. ; Tabusadani, M. ; Yamane, K. ; Takao, S. ; Kuroyama, Y. ; Mori, K. ; Ono, K. ; Kawahara, K. ; Omatsu, S. ; Furuuchi, K. ; Fujiwara, K. ; Morimoto, K. ; Kimura, H. ; Senjyu, HCitation: The International Journal of Tuberculosis and Lung Disease, 26(4), pp. 310-316; 2022Nagasaki University (長崎大学), 博士(医学) (2023-03-20)doctoral thesi
Decreased incremental shuttle walk test distance characterized by fibrocavitary lesions in non-tuberculous mycobacterial pulmonary disease
No full textNagasaki University (長崎大学)博士(医学)Background: Patients with non-tuberculous mycobacterial pulmonary disease (NTM-PD) have impaired exercise capacity, but the underlying factors are unknown. We investigated the characteristics of patients with NTM-PD and impaired exercise capacity. Methods: In total, 149 patients with NTM-PD participated in this study. Patients completed the incremental shuttle walk test (ISWT) to assess exercise capacity. Peripheral muscle strength and pulmonary function were also assessed. Radiological findings were classified into three phenotypes: non-cavitary nodular bronchiectatic (NC-NB) form, cavitary nodular bronchiectatic form, and fibrocavitary (FC) form. Results: The median ISWT distance (ISWD) and %ISWD were 450 meters and 88%. Participants were classified into three groups according to %ISWD, with %ISWD 80% as the normal or mildly decreased group. In a comparison of %ISWD among phenotypes, FC form had significantly lower %ISWD than those with NC-NB form. In the severely decreased group, peripheral muscle strength and pulmonary function were significantly lower than the other two groups. From a radiological standpoint, significantly more patients had FC form in the group with severely decreased %ISWD. Conclusions: Decreased ISWD is characterized by a deterioration in physical function and the presence of FC lesions in NTM-PD.長崎大学学位論文 学位記番号:博(医歯薬)甲第1573号 学位授与年月日:令和6年3月19日Author: Kazuki Ono, Mitsuru Tabusadani, Kazumasa Yamane, Satoshi Takao, Kosuke Mori, Yusuke Matsumura, Yuku Kuroyama, Kazuma Kawahara, Shunya Omatsu, Koji Furuuchi, Keiji Fujiwara, Kozo Morimoto, Hiroshi Kimura & Hideaki SenjyuCitation: Expert Review of Respiratory Medicine, 16(4), pp.469–475; 2022Nagasaki University (長崎大学), 博士(医学) (2024-03-19)doctoral thesi
Effects of pain on depression, sleep, exercise tolerance, and quality of life in patients with nontuberculous mycobacterial pulmonary disease
No full textThe experience and causes of pain in patients with nontuberculous mycobacterial pulmonary disease (NTM-PD) have not been clarified.
This study aimed to determine the prevalence and severity of bodily pain (BP) in patients with NTM-PD. We also investigated the clinical indicators that contribute to pain.
We used a retrospective cross-sectional study design. The participants were 114 NTM-PD patients (109 women) with a mean age of 65 years. The prevalence and severity of pain were measured using 2 items from the 36-Item Short Form Survey version 2 (SF-36), and the BP score was calculated. Functional limitation due to dyspnea was quantified using the Modified Medical Research Council Dyspnea Scale (mMRC), depression was assessed using the Center for Epidemiological Studies Depression Scale (CES-D), sleep quality was assessed using the Pittsburgh Sleep Quality Index (PSQI); health-related quality of life was assessed using the Leicester Cough Questionnaire (LCQ), and exercise tolerance was measured using the Incremental Shuttle Walk Test (ISWT).
Pain was reported by 70.2% of the patients (n = 80), and of these, 35.7% (n = 25) reported moderate to very severe pain. NTM-PD patients with high levels of pain had significantly higher scores on the mMRC, CES-D, and PSQI scores, and significantly lower performance on the ISWT and LCQ. Multiple regression analysis identified ISWT, CES-D, and PSQI as independent factors that affected BP scores.
Our findings suggest that pain significantly impacts daily life associated with reduced exercise tolerance, the presence of depressive symptoms, and poor sleep quality in patients with NTM-PD.長崎大学学位論文 学位記番号:博(医歯薬)甲第1542号 学位授与年月日:令和5年9月20日Author: Mori, Kosuke; Tabusadani, Mitsuru; Yamane, Kazumasa; Takao, Satoshi; Kuroyama, Yuki; Matsumura, Yusuke; Ono, Kazuki; Kawahara, Kazuma; Omatsu, Shunya; Fujiwara, Keiji; Furuuchi, Koji; Morimoto, Kozo; Kimura, Hiroshi; Senjyu, HideakiCitation: Medicine, 100(23), art. no. e26249; 2021Nagasaki University (長崎大学), 博士(医学) (2023-09-20
Novel Screening System of Virulent Strains for the Establishment of a Mycobacterium avium Complex Lung Disease Mouse Model Using Whole-Genome Sequencing
No full textNagasaki University (長崎大学)博士(医学)The establishment of animal models reflecting human Mycobacterium avium complex (MAC) lung disease (LD) pathology has the potential to expand our understanding of the disease pathophysiology. However, inducing sustained infection in immunocompetent mice is difficult since MAC generally shows less virulence and higher genetic variability than M. tuberculosis. To overcome this hurdle, we developed a screening system for identifying virulent MAC strains using whole-genome sequencing (WGS). We obtained nine clinical strains from Mycobacterium avium complex lung disease (MAC-LD) patients and divided them into two groups to make the mixed strain inocula for infection. Intranasal infection with the strain mixture of both groups in BALB/c mice resulted in progressive infection and extensive granuloma formation in the lungs, suggesting the existence of highly pathogenic strains in each group. We hypothesized that the change in the abundance of strain-specific single-nucleotide variants (SNVs) reflects the change in bacterial number of each strain in infected lungs. Based on this hypothesis, we quantified individual strain-specific SNVs in bacterial DNA from infected lungs. Specific SNVs for four strains were detected, suggesting the pathogenicity of these four strains. Consistent with these results, individual infection with these four strains induced a high lung bacterial burden, forming extensive peribronchial granuloma, while the other strains showed a decreased lung bacterial burden. The current method combining mixed infection and WGS accurately identified virulent strains that induced sustained infection in mice. This method will contribute to the establishment of mouse models that reflect human MAC-LD and lead to antimycobacterial drug testing.長崎大学学位論文 学位記番号:博(医歯薬)甲第1572号 学位授与年月日:令和6年3月19日Author: Koji Furuuchi, Shintaro Seto, Hajime Nakamura, Haruka Hikichi, Akiko Miyabayashi, Keiko Wakabayashi, Kazue Mizuno, Teruaki Oka, Kozo Morimoto, Minako Hijikata, Naoto KeichoCitation: Microbiology Spectrum, 10(3), art. no. e00451-22; 2022Nagasaki University (長崎大学), 博士(医学) (2024-03-19)doctoral thesi
