6 research outputs found
Synthesis and Characterisation of Novel Ruthenium Arene Complexes bearing Trichlorostannyl and Trichlorogermyl Groups as Potential Anti-Cancer Agents
No full textThe impact of physiotherapy on the balance of patients with vestibular neuritis: an extended literature review.
No full textVilnius University Faculty of Medicine Health Science Institute Department of Rehabilitation, Physical and Sports Medicine Bachelor’s Degree of Physiotherapy THE IMPACT OF PHYSIOTHERAPY ON THE BALANCE OF PATIENTS WITH VESTIBULAR NEURITIS: AN EXTENDED LITERATURE REVIEW Physiotherapy Bachelor’s Thesis The Author: Monika Jurgaityte. Academic supervisor: lecturer, doctor of physical medicine and sports rehabilitation, Teresė Palšytė. Keywords: vestibular neuritis, vestibular dysfunction, vestibular neuritis treatment, balance disorder, vestibular physiotherapy, vestibular rehabilitation, balance physiotherapy. The aim of research work: to analyse the effect of physiotherapy on the imbalance in patients with vestibular neuritis Tasks of work: 1. To analyze the impact of vestibular neuritis symptoms on people. 2. To analyze what physiotherapy methods are applied to people suffering from vestibular neuritis. 3. To analyze the effect of physiotherapy methods: adaptation, virtual reality, Tai Chi and water physiotherapy on balance in people with vestibular neuritis. Materials and methods: The extended literature review was carried out between October 2022 and March 2023. Scientific publications were searched in PubMed; Google Scholar; Springer; PTJ Physical Therapy& Rehabilitation Journal; Frontiers; Wiley Online Library; JNPT Journal of Neurologic Physical Therapy; BMC Neurology; The Cochrane Library and Research Gate database. Scientific articles were selected according to inclusion and exclusion criteria. About 122 articles, which met the study selection criteria, were selected for further evaluation. Conclusions: 1. People suffering from vestibular neuritis have the most impaired balance. Due to that, the quality of life is negatively affected. 2. In the case of vestibular neuritis, such physiotherapy methods as adaptations, aquatic physiotherapy, virtual reality and Tai Chi are applied. 3. Physiotherapy methods such as gaze stabilization, aquatic physiotherapy, virtual reality and Tai Chi improve balance and quality of life and reduce the symptoms caused by the disease
Modulation of the solubility properties of arene ruthenium complexes bearing stannyl ligands as potential anti-cancer agents
No full textCleavage of the known ruthenium dimer [RuCl2(η6-C6H5OCH2CH2OH)]2(1), bearing a hydrophilic substituent on the η6 coordinated aromatic ring, with the phosphine ligands: triphenyl phosphine, triphenyl phosphite, trimethyl phosphite, and 1,3,5-triaza-7-phosphaadamantane (PTA) afforded the known complexes [RuCl2(η6-C6H5OCH2CH2OH)(PPh3)] (2a), [RuCl2(η6-C6H5OCH2CH2OH){P(OPh)3}] (2b), [RuCl2(η6-C6H5OCH2CH2OH){P(OMe3)}] (2c), and [RuCl2 (η6-C6H5OCH2CH2OH)(PTA)] (4). The reaction of the known complex 2a with SnCl2afforded, by facile insertion of the SnCl2moiety into the Ru-Cl bond, the novel complex [RuCl(η6-C6H5OCH2CH2OH)(PPh3)(SnCl3)] (3a). Similarly, the reaction of complex 2b with SnCl2afforded the novel complex [RuCl(η6-C6H5OCH2CH2OH){P(OPh)3}(SnCl3)] (3b). Complexes 3a and 3b were fully characterized by spectroscopy (Infrared (IR) -spectroscopy, 1H, 31P and 119Sn Nuclear Magnetic Resonance (NMR) spectroscopy, UV-Vis spectroscopy and high resolution ESI-MS) and their thermal behaviour elucidated by Thermogravimetric Analysis (TGA). Density Functional Theory (DFT) calculations (Level of theory B3LYP, basis set for H, C, P, O, N and Cl is 6-31+G(d,p) and for Ru and Sn is DGDZVP) for complex 3a, 3b and 4 were also carried out, in particular to elucidate the bonding situation between Ru and Sn in complexes. The hitherto unprecedented anti-cancer activity of the complexes 2a – 2c as well as the novel stannyl complexes 3a and 3b were evaluated against MCF-7 (oestrogen receptor positive) human breast adenocarcinoma cell lines. All complexes show activity active against MCF-7 cell lines, indicating potential application as an anti-tumor agent
Biomimetic Bacterial Identification Platform Based on Thermal Transport Analysis Through Surface Imprinted Polymers: From Proof of Principle to Proof of Application
No full textAccurate and sensitive detection of bacteria is crucial in medicine for diagnosis and effective treatment of infectious diseases. Current state-of-the art methods consist of either traditional time consuming microbiological analysis or rapid, sensitive molecular techniques that require expensive readout equipment. In previous work, the authors of this paper combined synthetic bacteria receptors, so-called surface imprinted polymers (SIPs), with a novel thermal biosensor readout methodology for the detection of bacteria in urine. In this follow-up study, the potential of the method for application in urinary tract infection (UTI) diagnosis is further studied. The reproducibility of the method is assessed by expanding the study and analyzing the sensor's performance in urine samples obtained from four healthy adults. The samples are spiked with increasing concentrations of Escherichia coli to obtain different dose-response curves. The results of this study show that the method is reproducible over the studied population and variables such as age, gender and osmolality do not seem to influence the test. All results fall within the previously established dynamic range of 10(4)-10(5) bacteria mL(-1) which fits well within the diagnostic window of classical microbiological UTI tests. Further tests conducted on a urine sample 24 h after spiking illustrate the problem with traditional microbiology tests as the sensor response has significantly decreased due to the presence of a significant amount of dead bacteria in the day-old sample. These results confirm that fast, point-of-care analysis of fresh urine samples is advantageous over classic laborious techniques in terms of accurate diagnosis
High-resolution cryo-EM of the human CDK-activating kinase for structure-based drug design
No full textInternational audienceRational design of next-generation therapeutics can be facilitated by high-resolution structures of drug targets bound to small-molecule inhibitors. However, application of structure-based methods to macromolecules refractory to crystallization has been hampered by the often-limiting resolution and throughput of cryogenic electron microscopy (cryo-EM). Here, we use high-resolution cryo-EM to determine structures of the CDK-activating kinase, a master regulator of cell growth and division, in its free and nucleotide-bound states and in complex with 15 inhibitors at up to 1.8 Å resolution. Our structures provide detailed insight into inhibitor interactions and networks of water molecules in the active site of cyclin-dependent kinase 7 and provide insights into the mechanisms contributing to inhibitor selectivity, thereby providing the basis for rational design of next-generation therapeutics. These results establish a methodological framework for the use of high-resolution cryo-EM in structure-based drug design
High-resolution cryo-EM of the human CDK-activating kinase for structure-based drug design
No full textAbstract Rational design of next-generation therapeutics can be facilitated by high-resolution structures of drug targets bound to small-molecule inhibitors. However, application of structure-based methods to macromolecules refractory to crystallization has been hampered by the often-limiting resolution and throughput of cryogenic electron microscopy (cryo-EM). Here, we use high-resolution cryo-EM to determine structures of the CDK-activating kinase, a master regulator of cell growth and division, in its free and nucleotide-bound states and in complex with 15 inhibitors at up to 1.8 Å resolution. Our structures provide detailed insight into inhibitor interactions and networks of water molecules in the active site of cyclin-dependent kinase 7 and provide insights into the mechanisms contributing to inhibitor selectivity, thereby providing the basis for rational design of next-generation therapeutics. These results establish a methodological framework for the use of high-resolution cryo-EM in structure-based drug design
