3,389 research outputs found
Detection of dengue virus replication in peripheral blood mononuclear cells from dengue virus type 2-infected patients by a reverse transcription-real-time PCR assay
No full textImaging of skin dermal thermal damage by multiphoton autofluroescence and second harmonic generation (SHG) microscopy
No full textDifferentiation of ovarian mucinous carcinoma and metastatic colorectal adenocarcinoma by immunostaining with beta-catenin.
No full textEvaluation of dermal thermal damage by multiphoton autofluorescence and second-harmonic-generation microscopy
No full textTangle-bearing neurons survive despite disruption of membrane integrity in a mouse model of tauopathy
Full text linkNeurofibrillary tangles (NFTs) are associated with neuronal loss and correlate with cognitive impairment in Alzheimer disease, but how NFTs relate to neuronal death is not clear. We studied cell death in Tg4510 mice that reversibly express P301L mutant human tau and accumulate NFTs using in vivo multiphoton imaging of neurofibrillary pathology, propidium iodide (PI) incorporation into cells, caspase activation, and DNA labeling. We first observed that in live mice, a minority of neurons were labeled with the caspase probe or with PI fluorescence. These markers of cell stress were localized in the same cells and appeared specifically within NFT-bearing neurons. Contrary to expectations, the PI-stained neurons did not die during a day of observation; the presence of Hoechst-positive nuclei in them on the subsequent day indicated that the NFT-associated membrane disruption, as suggested by PI staining, and caspase activation do not lead to immediate death of neurons in this tauopathy model. This unique combination of in vivo multiphoton imaging with markers of cell death and pathological alteration is a powerful tool for investigating neuronal damage associated with neurofibrillary pathology
ANALIZA TV-DNEVNIKA KAO PORUKE
Full text linkUvodeći dva pojma (proceduralna efikasnost i proceduralna efektivnost) analiziramo TV-dnevnik kao poruku s komunikativnog aspekta. Paralelna analiza triju nacionalnih Tl'-dnevnika (BBC,ARD i TVZagreb) prikazuje različite načine na koje svaki od 3 analizirana nacionalna TV-dnevnika teži ili prema proceduralnoj efikasnosti ili proceduralnoj efektivnosti i upućuje na određene razlike u načinu realizacije TV-dnevnika
Upper rim allyl- and arylazo-coupled calix[4]arenes as highly sensitive chromogenic sensors for Hg2+ ion
No full textThe charge transfer and ion formation in liquid Li-Tl alloys
Full text linkThe 7Li NMR Knight shift, K, and the spin-lattice relaxation time, T1, were measured for liquid Li–Tl alloys. The K decreases rapidly with the addition of Tl up to 20 at.% Tl. In the concentration from 20 to 50 at.% Tl, the K decreases only slightly and the K of 50 at.% Tl is 60% of K for the pure liquid Li. Such a decrease of K is considered as an indication for the strong charge transfer from Li to Tl. These tendencies are similar to those from previous studies for liquid Li–Ga and Li–In alloys. However, beyond 50 at.% Tl, the K increases and reaches to an almost constant value (70% of K for the pure liquid Li). Such a back donation of charge is absent for liquid Li–Ga and Li–In alloys. It is considered that the tendency of the formation of ionic structural unit for liquid Li–Tl alloys is slightly weaker compared with the cases of liquid Li–Ga and Li–In alloys. The T1 is also discussed with the relation to the Knight shift and the electronic properties
THE VISIBLE EMISSION SPECTRA OF Zn , Cd , Zn Tl, AND Cd Tl MOLECULES
No full textAuthor Institution: Department of Physics, Indian Institute of Technology; Department of Physics, State University of New York at BuffaloNew Spectra have been obtained for Zn-Tl and Cd-Tl mixtures excited by a high frequency oscillator and studied under a dispersion of 5\AA/mm. Both the mixtures show one system in the red, one in the blue and continuous bands attached to the Tl lines 3775 and 5350 \AA. The red system of Zn-Tl mixture exhibits an isotope effect, which shows Zn as the emitter. The emitter for the red system of Cd-Tl mixture is Cd . The blue systems and continuous bands are due to diatomic ZnTl and CdTl molecules. A vibrational analysis of all these systems, wherever possible has been carried out and the constants determined
Dužina telomera kao biomarker starenja i bolesti
Full text linkAs research related to healthspan and lifespan has become a hot topic, the necessity for a
reliable and practical biomarker of aging (BoA), which can provide information about mortality
and morbidity risk, along with remaining life expectancy, has increased. The chromosome
terminus non-coding protective structure that prevents genomic instability is called a telomere.
The continual shortening of telomeres, which affects their structure as well as function, is a
hallmark of agedness. The aforementioned process is a potential cause of age-related diseases
(ARDs), leading to a bad prognosis and a low survival rate, which compromise health and
longevity. Hence, studies scrutinizing the BoAs often include telomere length (TL) as a
prospective candidate. The results of these studies suggest that TL measurement can only provide
an approximate appraisal of the aging rate, and its implementation into clinical practice and
routine use as a BoA has many limitations and challenges. Nevertheless, measuring TL while
determining other biomarkers can be used to assess biological age. This review focuses on the
importance of telomeres in health, senescence, and diseases, as well as on summarizing the results
and conclusions of previous studies evaluating TL as a potential BoA.Kako su istraživanja na temu starenja postala sve popularnija, javila se potreba za pouzdanim i praktičnim biomarkerom starenja, koji može pružiti informacije o riziku od mortaliteta i morbiditeta. Telomere su nekodirajući krajevi linearnih hromozoma koji održavaju njihovu stabilnost i integritet. Ćelijsko starenje i starenje organizma karakteriše progresivno skraćivanje telomera, što ugrožava njihovu strukturu i funkciju. Skraćivanje telomera je u vezi sa povećanom incidencom bolesti povezanih sa starenjem i lošom stopom preživljavanja, što ugrožava zdravlje i skraćuje životni vek. Stoga je dužina telomera dugo vremena prepoznata kao jedan od najboljih biomarkera starenja. Međutim, nedavna istraživanja ukazuju na to da dužina telomera može da pruži samo približnu procenu brzine starenja, pa je implementacija ovog biomarkera u kliničku praksu i rutinsku primenu praćena mnogim ograničenjima i izazovima. Uprkos tome, merenje dužine telomera, uz istovremeno određivanje drugih biomarkera, može poslužiti za procenu biološke starosti. Fokus ovog rada je na značaju telomera u ljudskom zdravlju, starenju i bolestima, kao i na sumiranju rezultata i zaključaka dosadašnjih studija koje su se bavile ispitivanjem dužine telomera kao potencijalnog kandidata za biomarker starenja
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