13 research outputs found
Geochemical Study on the Annual Variation of Oxygen Isotope and Chemical Composition of Groundwater in the Sho River Alluvium Fan, Toyama, Japan, as an Investigation of Selected Qualitative Aspects of Efficient Utilization of Groundwater Heat
No full textSeasonal variations of water temperature, electric conductivity, and oxygen isotope and chemical composition of shallow groundwaters and river waters were determined in the Sho River alluvial fan, western Toyama Prefecture, Japan, to examine groundwater heat utilization for indoor climate control. Samples were collected at 31 sites every 2 months for 1 year and at 11 representative sites monthly. In addition, the results of monthly precipitation amount and oxygen isotope composition of precipitation collected within the region during the same period were also taken into account. The sources of the shallow groundwaters are a mixture of river water and precipitation. The contribution of precipitation to groundwater is generally small along the Sho River but reaches as much as 80% along the Oyabe River and in the south and west of the alluvial fan. Though the origin of the groundwater differs regionally, water temperature is fixed at around 15 °C throughout the year in the northern part of the alluvial fan, and open-type ground source heat pump systems can be used for cooling and heating there, if adequate quantitative aquifer properties (exploitable groundwater amounts) are present
Geochemical Study of Groundwater in the Sho River Fan, Toyama Prefecture for Heat Usage by a Geothermal Heat Pump
No full textAbstractChemical and isotopic (D, O) compositions of 56 water samples from the Sho River fan, Toyama, northern part of central Japan, were analyzed to examine their water quality, origins, and water flow for geothermal heat extraction used for air-conditioning and melting of road snow by a geothermal heat pump (Geo-HP). Groundwaters are a mixture of two big river waters (Sho and Oyabe) and precipitation. Deep groundwaters from observation wells are characterized to be high in pH and enriched in HCO3 compared to the shallow groundwaters. These features may indicate that the shallow groundwater originated from a mixture of river water and precipitation moving to the north and becoming confined due to the presence of an impermeable layer. Groundwaters attain high pH due to ion exchange reactions with rocks containing clay minerals, where HCO3 concentration also increases
脂肪滴はヒト肝細胞株におけるPorphyromonas gingivalisのオートファジー・リソソームシステムを介した排除機構に影響する
No full textRecent studies have shown that infection with Porphyromonas gingivalis, a major periodontal pathogen, hastens the progression of non-alcoholic fatty liver disease (NAFLD). However, the intracellular fate of P. gingivalis in hepatocytes remains unknown. Here, using oleic-acid-induced HepG2 cells as an in vitro model for NAFLD, we found that lipid droplets increased the existence of P. gingivalis in the cells at an early phase of infection. Confocal microscopic analysis revealed that lipid droplets affected the formation of autolysosomes in infected cells. Thus, lipid droplets affect the elimination of P. gingivalis in HepG2 cells by altering the autophagy-lysosome system.長崎大学学位論文 学位記番号:博(医歯薬)甲第916号 学位授与年月日:平成29年3月21日Author: Yumi Zaitsu, Mayumi Iwatake, Keiko Sato, Takayuki TsukubaCitation: Microbes and Infection, 18(9), pp.565-571;201
Actin binding LIM 1 (abLIM1)は破骨細胞における細胞遊走と融合を制御することで破骨細胞分化を負に制御する
No full textActin binding LIM 1 (abLIM1) is a cytoskeletal actin-binding protein that has been implicated in interactions between actin filaments and cytoplasmic targets. Previous biochemical and cytochemical studies have shown that abLIM1 interacts and co-localizes with F-actin in the retina and muscle. However, whether abLIM1 regulates osteoclast differentiation has not yet been elucidated. In this study, we examined the role of abLIM1 in osteoclast differentiation and function. We found that abLIM1 expression was upregulated during receptor activator of nuclear factor kappa-B ligand (RANKL)-induced osteoclast differentiation, and that a novel transcript of abLIM1 was exclusively expressed in osteoclasts. Overexpression of abLIM1 in the murine monocytic cell line, RAW-D suppressed osteoclast differentiation and decreased expression of several osteoclast-marker genes. By contrast, small interfering RNA-induced knockdown of abLIM1 enhanced the formation of multinucleated osteoclasts and markedly increased the expression of the osteoclast-marker genes. Mechanistically, abLIM1 regulated the localization of tubulin, migration, and fusion in osteoclasts. Thus, these results indicate that abLIM1 negatively controls osteoclast differentiation by regulating cell migration and fusion mediated via actin formation.長崎大学学位論文 学位記番号:博(医歯薬)甲第1079号 学位授与年月日:平成30年6月6日Author: Haruna Narahara, Eiko Sakai, Yu Yamaguchi, Shun Narahara, Mayumi Iwatake, Kuniaki Okamoto, Noriaki Yoshida, Takayuki TsukubaCitation: Journal of Cellular Physiology, 234(1), pp.486-499; 201
Spheroid morphology of lung cancer cell lines correlates with oncological profiles
No full textNagasaki University (長崎大学)博士(医学)We assessed the correlation between Multicellular tumor spheroids (MCTS) morphology and the oncological profile of lung cancer cells. MCTS were generated in five lung cancer cell lines and classified into Type–A MCTS, which showed strong aggregation, and Type–B MCTS, which showed weak aggregation. Drug resistance was higher in Type–A MCTS, and invasive ability was higher in Type–B MCTS. The oncologic profile of lung cancer cell lines correlated with MCTS morphology. MCTS morphology could thus be used in basic oncology research and as a clinical prognostic tool.長崎大学学位論文 学位記番号:博(医歯薬)甲第1708号 学位授与年月日:令和7年6月4日Author: Kaido Oishi, Keitaro Matsumoto, Shintaro Hashimoto, Fumitake Uchida, Ryosuke Hara, Masato Nishimuta, Takamune Matsumoto, Mayumi Iwatake, Koichi Tomoshige, Ryoichiro Doi, Ryusuke Machino, Tomohiro Obata, Takeshi NagayasuCitation: Discover Oncology, 16, art. no. 627: 2025Nagasaki University (長崎大学), 博士(医学) (2025-06-04)doctoral thesi
Chemical composition of fog water at Mt. Tateyama near the coast of the Japan Sea in central Japan
No full textAdipose-Derived Mesenchymal Stem Cells Attenuate Immune Reactions Against Pig Decellularized Bronchi Engrafted into Rat Tracheal Defects
No full textNagasaki University (長崎大学)博士(医学)Decellularized scaffolds are promising biomaterials for tissue and organ reconstruction; however, strategies to effectively suppress the host immune responses toward these implants, particularly those without chemical crosslinking, remain warranted. Administration of mesenchymal stem cells is effective against immune-mediated inflammatory disorders. Herein, we investigated the effect of isogeneic abdominal adipose-derived mesenchymal stem/stromal cells (ADMSCs) on xenogeneic biomaterial-induced immunoreactions. Peripheral bronchi from pigs, decellularized using a detergent enzymatic method, were engrafted onto tracheal defects of Brown Norway (BN) rats. BN rats were implanted with native pig bronchi (Xenograft group), decellularized pig bronchi (Decellularized Xenograft), or Decellularized Xenograft and ADMSCs (Decellularized Xenograft +ADMSC group). In the latter group, ADMSCs were injected intravenously immediately post implantation. Harvested graft implants were assessed histologically and immunohistochemically. We found that acute rejections were milder in the Decellularized Xenograft and Decellularized Xenograft+ADMSC groups than in the Xenograft group. Mild inflammatory cell infiltration and reduced collagen deposition were observed in the Decellularized Xenograft+ADMSC group. Additionally, ADMSC administration decreased CD8+ lymphocyte counts but increased CD163+ cell counts. In the Decellularized Xenograft+ADMSC group, serum levels of vascular endothelial growth factor and IL-10 were elevated and tissue deposition of IgM and IgG was low. The significant immunosuppressive effects of ADMSCs illustrate their potential use as immunosuppressive agents for xenogeneic biomaterial-based implants.長崎大学学位論文 学位記番号:博(医歯薬)甲第1559号 学位授与年月日:令和5年12月6日Author: Makoto Hisanaga, Tomoshi Tsuchiya, Hironosuke Watanabe, Koichiro Shimoyama, Mayumi Iwatake, Yukinori Tanoue, Keizaburo Maruyama, Hiroshi Yukawa, Kazuhide Sato, Yoshimi Kato, Keitaro Matsumoto, Takuro Miyazaki, Ryoichiro Doi, Koichi Tomoshige & Takeshi NagayasuCitation: Organogenesis, 19(1), art. no. 2212582; 2023Nagasaki University (長崎大学), 博士(医学) (2023-12-06)doctoral thesi
Immunomodulatory Macrophages Enable E-MNC Therapy for Radiation-Induced Salivary Gland Hypofunction
No full textNagasaki University (長崎大学)博士(歯学)A newly developed therapy using effective-mononuclear cells (E-MNCs) is reportedly effective against radiation-damaged salivary glands (SGs) due to anti-inflammatory and revascularization effects. However, the cellular working mechanism of E-MNC therapy in SGs remains to be elucidated. In this study, E-MNCs were induced from peripheral blood mononuclear cells (PBMNCs) by culture for 5–7 days in medium supplemented with five specific recombinant proteins (5G-culture). We analyzed the anti-inflammatory characteristics of macrophage fraction of E-MNCs using a co-culture model with CD3/CD28-stimulated PBMNCs. To test therapeutic efficacy in vivo, either E-MNCs or E-MNCs depleted of CD11b-positive cells were transplanted intraglandularly into mice with radiation-damaged SGs. Following transplantation, SG function recovery and immunohistochemical analyses of harvested SGs were assessed to determine if CD11b-positive macrophages contributed to tissue regeneration. The results indicated that CD11b/CD206-positive (M2-like) macrophages were specifically induced in E-MNCs during 5G-culture, and Msr1- and galectin3-positive cells (immunomodulatory macrophages) were predominant. CD11b-positive fraction of E-MNCs significantly inhibited the expression of inflammation-related genes in CD3/CD28-stimulated PBMNCs. Transplanted E-MNCs exhibited a therapeutic effect on saliva secretion and reduced tissue fibrosis in radiation-damaged SGs, whereas E-MNCs depleted of CD11b-positive cells and radiated controls did not. Immunohistochemical analyses revealed HMGB1 phagocytosis and IGF1 secretion by CD11b/Msr1-positive macrophages from both transplanted E-MNCs and host M2-macrophages. Thus, the anti-inflammatory and tissue-regenerative effects observed in E-MNC therapy against radiation-damaged SGs can be partly explained by the immunomodulatory effect of M2-dominant macrophage fraction.長崎大学学位論文 学位記番号:博(医歯薬)甲第1549号 学位授与年月日:令和5年9月6日Author: Ryo Honma, Takashi I, Makoto Seki, Mayumi Iwatake, Takunori Ogaeri, Kayo Hasegawa, Seigo Ohba, Simon D. Tran, Izumi Asahina and Yoshinori SumitaCitation: Cells, 12(10), art. no. 1417; 2023Nagasaki University (長崎大学), 博士(歯学) (2023-09-06)doctoral thesi
A novel ex vivo lung cancer model based on bioengineered rat lungs
No full textNagasaki University (長崎大学)博士(医学)Introduction: Two-dimensional cell cultures have contributed substantially to lung cancer research, but 3D cultures are gaining attention as a new, more efficient, and effective research model. A model reproducing the 3D characteristics and tumor microenvironment of the lungs in vivo, including the co-existence of healthy alveolar cells with lung cancer cells, is ideal. Here, we describe the creation of a successful ex vivo lung cancer model based on bioengineered lungs formed by decellularization and recellularization. Methods: Human cancer cells were directly implanted into a bioengineered rat lung, which was created with a decellularized rat lung scaffold reseeded with epithelial cells, endothelial cells and adipose-derived stem cells. Four human lung cancer cell lines (A549, PC-9, H1299, and PC-6) were applied to demonstrate forming cancer nodules on recellularized lungs and histopathological assessment were made among these models. MUC-1 expression analysis, RNA-seq analysis and drug response test were performed to demonstrate the superiority of this cancer model. Results: The morphology and MUC-1 expression of the model were like those of lung cancer in vivo. RNA sequencing revealed an elevated expression of genes related to epithelial-mesenchymal transition, hypoxia, and TNF-α signaling via NF-κB; but suppression of cell cycle-related genes including E2F. Drug response assays showed that gefitinib suppressed PC-9 cell proliferation equally well in the 3D lung cancer model as in 2D culture dishes, albeit over a smaller volume of cells, suggesting that fluctuations in gefitinib resistance genes such as JUN may affect drug sensitivity. Conclusions: A novel ex vivo lung cancer model was closely reproduced the 3D structure and microenvironment of the actual lungs, highlighting its possible use as a platform for lung cancer research and pathophysiological studies.長崎大学学位論文 学位記番号:博(医歯薬)甲第1543号 学位授与年月日:令和5年9月6日Author: Satoshi Mizoguchi, Tomoshi Tsuchiya, Ryoichiro Doi, Tomohiro Obata, Mayumi Iwatake, Shintaro Hashimoto, Hirotaka Matsumoto, Hiroshi Yukawa, Hiroko Hayashi, Tao-Sheng Li, Kazuko Yamamoto, Keitaro Matsumoto, Takuro Miyazaki, Koichi Tomoshige and Takeshi NagayasuCitation: Frontiers in Bioengineering and Biotechnology, 11, art. no. 1179830; 2023Nagasaki University (長崎大学), 博士(医学) (2023-09-06)doctoral thesi
Effective-mononuclear cell (E-MNC) therapy alleviates salivary gland damage by suppressing lymphocyte infiltration in Sjögren-like disease
No full textNagasaki University (長崎大学)博士(歯学)Introduction: Sjögren syndrome (SS) is an autoimmune disease characterized by salivary gland (SG) destruction leading to loss of secretory function. A hallmark of the disease is the presence of focal lymphocyte infiltration in SGs, which is predominantly composed of T cells. Currently, there are no effective therapies for SS. Recently, we demonstrated that a newly developed therapy using effective-mononuclear cells (E-MNCs) improved the function of radiation-injured SGs due to anti-inflammatory and regenerative effects. In this study, we investigated whether E-MNCs could ameliorate disease development in non-obese diabetic (NOD) mice as a model for primary SS. Methods: E-MNCs were obtained from peripheral blood mononuclear cells (PBMNCs) cultured for 7 days in serum-free medium supplemented with five specific recombinant proteins (5G culture). The anti-inflammatory characteristics of E-MNCs were then analyzed using a co-culture system with CD3/CD28-stimulated PBMNCs. To evaluate the therapeutic efficacy of E-MNCs against SS onset, E-MNCs were transplanted into SGs of NOD mice. Subsequently, saliva secretion, histological, and gene expression analyses of harvested SG were performed to investigate if E-MNCs therapy delays disease development. Results: First, we characterized that both human and mouse E-MNCs exhibited induction of CD11b/CD206-positive cells (M2 macrophages) and that human E-MNCs could inhibit inflammatory gene expressions in CD3/CD28- stimulated PBMNCs. Further analyses revealed that Msr1-and galectin3-positive macrophages (immunomodulatory M2c phenotype) were specifically induced in E-MNCs of both NOD and MHC class I-matched mice. Transplanted E-MNCs induced M2 macrophages and reduced the expression of T cell-derived chemokine-related and inflammatory genes in SG tissue of NOD mice at SS-onset. Then, E-MNCs suppressed the infiltration of CD4-positive T cells and facilitated the maintenance of saliva secretion for up to 12 weeks after E-MNC administration. Discussion: Thus, the immunomodulatory actions of E-MNCs could be part of a therapeutic strategy targeting the early stage of primary SS.長崎大学学位論文 学位記番号:博(医歯薬)甲第1554号 学位授与年月日:令和5年9月20日Author: Kayo Hasegawa, Jorge Luis Montenegro Raudales, Takashi I, Takako Yoshida, Ryo Honma, Mayumi Iwatake, Simon D. Tran, Makoto Seki, Izumi Asahina and Yoshinori SumitaCitation: Frontiers in Bioengineering and Biotechnology, 11, 1144624; 2023Nagasaki University (長崎大学), 博士(歯学) (2023-09-20)doctoral thesi
