112,183 research outputs found
Identification of EBNA binding cellular proteins, using yeast two-hybrid system
No full textEpstein-Barr virus (EBV) is a common herpesvirus that establishes life-long persistence in the human host through the elaborate regulation of different latency types. Latent EBV infection of resting B cells converts them into transformed cells that can develop into tumors in immune-compromised hosts. As such, EBV infection is a unique, well-defined in vitro system for malignant transformation. The latency-associated EBV proteins are the key factors for virus-induced cell transformation. To fully understand the molecular mechanisms that lead to virusinduced transformation, the cellular targets of the transforming viral proteins have to be identified.During the work summarized in this thesis, the following EBV encoded nuclear antigen (EBNA) binding proteins were identified: epsilon-subunit of the human chaperonin TCP- 1 complex; XAP2, the minor subunit of the arylhydrocarbon receptor (AhR) complex; a novel human uridine kinase/uracil phosphoribosyltransferase (for EBNA-3); and p14ARF (for EBNA-5). Epsilon-subunit of the human chaperonin TCP-1 complex is part of a huge protein complex that helps to fold the newly synthesized polypeptides. We mapped the interacting region to the apical domain of the epsilon-subunit the most likely site for recognition of newly translated polypeptides. We concluded that nascent EBNA-3 might receive help for its folding from the TCP-1 complex. Another protein that binds to EBNA-3 is XAP-2, which also interacts with the transformation associated X-protein encoded by the hepatitis B virus. We showed that EBNA-3 induces translocation of the cytoplasmic XAP-2 to the nucleus. We also found a previously unknown EBNA-3 binding protein, which was designated F538.We have shown that the predominantly cytoplasmic F538 relocated to the nucleus in the presence of EBNA-3, where these two proteins showed high levels of co-localization. A natural splice variant with the deleted C-terminus of F538 did not translocate to the nucleus. A SWISSModel 3D structure of F538 was constructed and compared with other known proteins. This raised the possibility that F538 is a novel human uridine kinase/uracil phosphoribosyltransferase (UK/UPRT). We suggested that EBNA-3 by direct protein- protein interaction induced the nuclear accumulation of this enzyme that is most likely part of the ribonucleotide salvage pathway. Increased intra-nuclear levels of UK/UPRT might contribute to the metabolic build-up that is needed for blast transformation and rapid proliferation.We found that EBNA-5 binds to p14ARF, one of the upstream regulators of the p53 pathway. We showed that EBNA-5 prolonged the survival of the p14ARFtransfected cells. We observed the accumulation of the p14ARF in extra-nuclear inclusions where it co-localized with p53, HDM2 and Hsp70. Formation of the p14ARF inclusions induced the translocation of PML bodies and 20S proteasome subunits. Co-expression of p14ARF and EBNA-5 led to the complete relocation of EBNA-5 into the p14ARF inclusions. We suggested that EBNA-5 might play a role in regulating the degradation of p14ARF-p53-HDM2 complexes.In conclusion, using the yeast two-hybrid system we found new cellular targets for EBV-encoded transformation associated latent proteins. Some of these target molecules participate in signal transduction (Xap-2); growth associated metabolic pathways (F538); cell cycle regulation and protein degradation (p14ARF).List of scientific papersI. Kashuba E, Pokrovskaja K, Klein G, Szekely L (1999). Epstein-Barr virus-encoded nuclear protein EBNA-3 interacts with the epsilon-subunit of the T-complex protein 1 chaperonin complex. J Hum Virol. 2(1): 33-7. https://pubmed.ncbi.nlm.nih.gov/10200597II. Kashuba E, Kashuba V, Pokrovskaja K, Klein G, Szekely L (2000). Epstein-Barr virus encoded nuclear protein EBNA-3 binds XAP-2, a protein associated with Hepatitis B virus X antigen. Oncogene. 19(14): 1801-6. https://pubmed.ncbi.nlm.nih.gov/10777214III. Pokrovskaja K, Mattsson K, Kashuba E, Klein G, Szekely L (2001). Proteasome inhibitor induces nucleolar translocation of Epstein-Barr virus-encoded EBNA-5. J Gen Virol. 82(Pt 2): 345-58. https://pubmed.ncbi.nlm.nih.gov/11161273IV. Kashuba E, Kashuba V, Sandalova T, Klein G, Szekely L (2002). Epstein-Barr virus encoded nuclear protein EBNA-3 binds a novel human uridine kinase/uracil phosphoribosyltransferase. BMC Cell Biol. 3(1): 23. https://pubmed.ncbi.nlm.nih.gov/12199906V. Kashuba E, Matsson K, Pokrovskaja K, Kiss C, Protopopova M, Ehlin-Henriksson B, Klein G, Szekely L (2002). EBV-encoded EBNA-5 associates with p14ARF in extranucleolar inclusions and prolongs the survival of p14ARF-expressing cells. [Submitted]VI. Kashuba E, Matsson K, Klein G, Szekely L (2002). Accumulation of p14ARF protein in extranucleolar inclusions induces the re-distribution of PML bodies and proteasomes. [Submitted]</p
author-bios-SRD-19-0063.R1 – Supplemental material for The Network Structure of Police Misconduct
No full textSupplemental material, author-bios-SRD-19-0063.R1 for The Network Structure of Police Misconduct by George Wood, Daria Roithmayr and Andrew V. Papachristos in Socius</p
Impact of Aquafitness Training on Physical Condition of Early Adulthood Women
No full textKashuba, V. Impact of Aquafitness Training on Physical Condition of Early Adulthood Women / V. Kashuba, O. Andrieieva, A. Hakman, I. Grygus, O. Smolenska, M. Ostrowska, M. Napierala, M. Hagner-Derengowska, R. Muszkieta, W. Zukow // Теорія та методика фізичного виховання. ─ 2021. ─ Том 21, № 2. ─ С. 152-157.The physical benefits of fitness for middle-aged women are well-known. Unlike the usual training sessions, aquafitness occupies a special place among the types of health-related exercise training. Its health-enhancing effect results from the activation of the body's functional systems, the gravitational unloading of the musculoskeletal system, high energy expenditures, and overall body hardening effect
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
No full text“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Practical implementation of the concept of health-forming technologies into the process of physical education of primary school age children
No full textKashuba V. Practical Implementation of the Concept of Health-Forming Technologies into the Process of Physical Education of Primary School Age Children / V. Kashuba, N. Goncharova, H. Butenko // Education, Health and Sport formerly Journal of Health Sciences. - Bydgoszcz, Poland. - 2018. - № 8(6). - P. 469-477. - Доступно: http://www.ojs.ukw.edu.pl/index.php/johs/article/view/6579.Summary
The article considers the burning problem of positive influence of active tourism means on morbidity indices and degree of body resistance of primary school age children against unfavorable factors of the environment. The objective is to give a description of the morbidity of 1-4 grades students, to determine the degree of resistance of their organism to unfavorable factors of the environment and to determine the nature of the impact of active tourism means on these indices at primary school students in the process of physical education. The study consisted of ascertaining and educational experiments, which lasted one academic year each. 265 students of 1-4 grades took part in the ascertaining experiment. 91 students of 1, 3, 4 grades were involved in educational experiment and were divided into experimental and control groups.
As a result of the ascertaining experiment, 55.09% of children with certain disorders in their health were identified, among which the diseases of the bone and muscular system predominate. It was established that the indices of the morbidity and degree of body resistance of children to unfavorable factors of the environment tend to deteriorate in the age dynamics. Children were characterized by low level of physical health. In order to determine the impact on these indices, the concept of health-forming technologies on the basis of active tourism means was developed. As a result of the educational experiment, it was established that children from experimental groups in comparison with children from control groups experienced a statistically significant improvement in the level of physical health, the degree of body resistance to unfavorable factors of the environment
Biomechanical analysis of skilled female gymnasts’ technique in “round-off, flic-flac” type on the vault table
No full textKashuba V. Biomechanical analysis of skilled female gymnasts’ technique in “round-off, flic-flac” type on the vault table / V. Kashuba, I. Khmelnitska, S. Krupenya // Journal of Physical Education and Sport (JPES). – 2012. - № 12(4). - Р. 431- 435
Induced Modules for Affine Lie Algebras
No full textWe study induced modules of nonzero central charge with arbitrary multiplicities over affine Lie algebras. For a given pseudo parabolic subalgebra P of an affine Lie algebra G, our main result establishes the equivalence between a certain category of P-induced G-modules and the category of weight P-modules with injective action of the central element of G. In particular, the induction functor preserves irreducible modules. If P is a parabolic subalgebra with a finite-dimensional Levi factor then it defines a unique pseudo parabolic subalgebra Pps, P Ì Pps. The structure of P-induced modules in this case is fully determined by the structure of Pps-induced modules. These results generalize similar reductions in particular cases previously considered by V. Futorny, S. König, V. Mazorchuk [Forum Math. 13 (2001), 641-661], B. Cox [Pacific J. Math. 165 (1994), 269-294] and I. Dimitrov, V. Futorny, I. Penkov [Comm. Math. Phys. 250 (2004), 47-63].This paper is a contribution to the Special Issue on Kac–Moody Algebras and Applications. The first author is supported in part by the CNPq grant (301743/2007-0) and by the Fapesp grant (2005/60337-2). The second author is supported by the Fapesp grant (2007/025861)
JETP Letters V. 76, I. 11
No full textJETP Letters -- December 10, 2002
Volume 76, Issue 11, pp. 647-692
FIELDS, PARTICLES, AND NUCLEI
Confining String Formed by P vortices in the Indirect Z(2) Projection of SU(2) Lattice Gauge Theory
V. G. Bornyakov, A. V. Kovalenko, M. I. Polikarpov, and D. A. Sigaev
pp. 647-650 Full Text: PDF (67 kB)
CONDENSED MATTER
Possible Universal Cause of High-Tc Superconductivity in Different Metals
M. Ya. Amusia and V. R. Shaginyan
pp. 651-655 Full Text: PDF (57 kB)
Spin-Wave Resonance in Three-Layer NiFe/DyxCo1 – x/NiFe Films as a Method for Detecting Structural Inhomogeneities in Amorphous DyxCo1 – x Layers
R. S. Iskhakov, V. A. Seredkin, S. V. Stolyar, L. A. Chekanova, and V. Yu. Yakovchuk
pp. 656-659 Full Text: PDF (68 kB)
Electron-Filling Kinetics of 2D States on a Helium Film
V. B. Shikin
pp. 660-663 Full Text: PDF (59 kB)
Magnetic Collapse and the Change of Electronic Structure of FeBO3 Antiferromagnet under High Pressure
V. A. Sarkisyan, I. A. Troyan, I. S. Lyubutin, A. G. Gavrilyuk, and A. F. Kashuba
pp. 664-669 Full Text: PDF (118 kB)
Observation of Subgap Resistive Oscillations in Doubly Connected SNS Systems with the Suppressed Proximity Effect
Yu. N. Chiang and O. G. Shevchenko
pp. 670-674 Full Text: PDF (83 kB)
MISCELLANEOUS
How Behavior of Systems with Sparse Spectrum Can Be Predicted on a Quantum Computer
Y. Ozhigov
pp. 675-680 Full Text: PDF (66 kB)
SCIENTIFIC SUMMARIES
Interplay between the Structure and Properties of New Metastable Carbon Phases Obtained under High Pressures from Fullerite C60 and Carbyne
V. V. Brazhkin, A. G. Lyapin, S. V. Popova, S. C. Bayliss, T. D. Varfolomeeva, R. N. Voloshin, A. G. Gavrilyuk, M. V. Kondrin, V. V. Mukhamad'yarov, I. A. Troyan, S. V. Demishev, A. A. Pronin, and N. E. Sluchanko
pp. 681-692 Full Text: PDF (171 kB)Archived web conten
Measures to Prevent Functional Muscular Disorders in Sports Training of 7-9- Year-Old Football Players
No full textKashuba, V. Measures to Prevent Functional Muscular Disorders in Sports Training of 7-9- Year-Old Football Players / V. Kashuba, O. Andrieieva, L. Yarmolinsky, I. Karp, V. Kyrychenko, Y. Goncharenko, T. Rychok, N. Nosova // Journal of Physical Education and Sport. ─ 2020. ─ Vol. 20. ─ P. 366 – 371.Дослідження було спрямоване на оцінку ефективності заходів щодо запобігання постуральним розладам юних футболістів на початкових етапах спортивної підготовки. У цьому дослідженні були використані такі методи: педагогічний експеримент; педагогічне тестування; антропометрика, відеозапис та біомеханічний аналіз біогеометричного профілю постави індивіда (за допомогою програмного забезпечення Torso), методи математичної статистики. У педагогічному експерименті брали участь юні спортсмени віком від 8 років з діагностованим розладом "кругла поза". Результати: Було встановлено, що у віковій групі 9 років відсоток спортсменів із постуральними порушеннями становить вдвічі більше, ніж у віковій групі 8 років і майже втричі більше, ніж у групі 7 років спортсменів. Була розроблена навчальна програма, яка включала коригувальні вправи, розділені на блоки: корекційна, профілактична, динамічна постава, вертикальна стійкість тіла, фізичні ігри та естафети та розтягування. Тривалість експерименту становила 1 рік. Проведений педагогічний експеримент підтвердив ефективність програми корекції постуральних розладів у молодих футболістів. Були відзначені позитивні зміни показників біогеометричного профілю постави та фізичної підготовленості юних футболістів
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