148 research outputs found
Biomolecular layer thickness evaluation using White Light Reflectance Spectroscopy
No full textA White Light Reflectance Spectroscopy (WLRS) methodology is applied in the evaluation of the effectiveness of biomolecules immobilization onto solid surfaces as well as their subsequent reaction with counterpart biomolecules via measuring the respective layers thickness. In particular we investigated the adsorption of rabbit and mouse gamma-globulins as well as their reaction with complementary antibodies. The results obtained with the proposed methodology were compared with those received by atomic force microscopy analysis of the same samples. It was found that the developed method provides a simple, very fast and accurate approach for thin biomolecular layers thickness determination
Surgical Infections
No full textSurgical infections are caused by the breakdown of the equilibrium existing between organisms and the host. This may occur after a breach in a protective surface, as occurs after surgical trauma, changes in host resistance, or particular characteristics of the organism. The possible outcomes are abscess formation, local spread with/without tissue death, distant spread or resolution. A surgical infection is an infection requiring operative treatment (excision or drainage), and occupies an unvascularized space in tissue, or may occur in an operated site. Common examples of the former group are furuncles and carbuncles, hollow viscus inflammations, such as appendicitis, cholecystitis, and most abscesses. The latter group comprises all surgical site infections. This Research Topic provides comprehensive information on the biology, mechanisms, prevention and treatment of surgery-related infections
Chemocapacitance response simulation through polymer swelling and capacitor modeling
No full textAbstractA methodology for the prediction of chemocapacitor response due to the sorption of analyte without the need for fabrication of the actual devices is introduced. The methodology is based on experimental measurement of volume fraction due to sorption of the analyte through reflectance measurements and electromagnetic modeling for the InterDigitated Electrode (IDE) capacitor. The volume fraction and the capacitance of the device are coupled through the use of Clausius-Mossotti equation for the calculation of dielectric constant of sorbed polymeric film resulting in the prediction of the capacitance change due to the sorption of the analyte
Fully integrated monolithic opoelectronic transducer for real.time protein and DNA detection:The NEMOSLAB appraoch
No full textThe development and testing of a portable bioanalytical device which was capable for real-time monitoring of binding assays was demonstrated. The device was based on arrays of nine optoelectronic transducers monolithically integrated on silicon chips. The optocouplers consisted of nine silicon avalanche diodes self-aligned to nine silicon nitride waveguides all converging to a single silicon detector. The waveguides were biofunctionalized by appropriate recognition molecules. Integrated thick polymer microchannels provided the necessary fluidic functions to the chip. A single sided direct contact scheme through a board-to-board receptacle was developed and combined with a portable customized readout and control instrument. Real-time detection of deleterious mutations in BRCA1 gene related to predisposition to hereditary breast/ovarian cancer was performed with the instrument developed using PCR products. Detection was based on waveguided photons elimination through interaction with fluorescently labeled PCR products. Detection of single biomolecular binding events was also demonstrated using nanoparticles as labels. In addition, label-free monitoring of bioreactions in real time was achieved by exploiting wavelength filtering on photonic crystal engineered waveguides. The proposed miniaturized sensing device with proper packaging and accompanied by a portable instrument can find wide application as a platform for reliable and cost effective point-of-care diagnosis
Three-dimensional simulation of carrier transport effects in the base of rear point contact silicon solar cells
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Computer simulation of transient experiments for determining the transport parameters in amorphous silicon solar cells
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