1,113 research outputs found
Expression, phosphorylation, and mRNA-binding of heterogeneous nuclear ribonucleoprotein K in Xenopus oocytes, eggs, and early embryos
No full textHere we show that heterogeneous nuclear ribonucleoprotein K (hnRNP K), a member of the K homology domain-containing proteins, is expressed in Xenopus immature oocytes, unfertilized eggs, and early embryos. Fertilization or egg activation treatment involving upregulation of the egg tyrosine kinase Src promotes a rapid and transient tyrosine phosphorylation of hnRNP K. HnRNP K is also phosphorylated on serine/threonine residues in unfertilized eggs, dephosphorylated after fertilization, and re-phosphorylated during the premitotic phase of early embryogenesis. In vitro, Src and mitogen-activated protein kinase (MAPK) were capable of phosphorylating hnRNP K on tyrosine and serine/threonine residues, respectively. In support of this, pretreatment of oocytes, eggs, or embryos with inhibitors for Src (PP2) and MAPK (U0126) blocked effectively the phosphorylation of hnRNP K. We also identify some maternal mRNAs that coimmuno-precipitate with hnRNP K in unfertilized eggs. Specific binding of these mRNAs to hnRNP K was verified by reverse transcriptase-polymerase chain reaction (RT-PCR). In addition, real-time PCR analyses revealed a subset of the mRNAs whose binding to hnRNP K might be up or downregulated in activated eggs. In vitro binding assay with the use of poly U monopolymeric RNA-coupled beads demonstrated that the RNA-binding property of hnRNP K is negatively regulated by tyrosine phosphorylation and positively or neutrally regulated by serine/threonine phosphorylation. Taken together, it is attractive to suggest that hnRNP K is in association with certain pools of maternal mRNAs whose translational activation are modulated by the Src/MAPK phosphorylation of hnRNP K during oocyte-egg-embryo transition
So Deep in the Mountains: Saigyo\u27s Yama fukami poems and Reclusion in Medieval Japanese Poetry
No full textExamining a set of poems exchanged by the monks Saigyō and Jakuzen, the author argues for their importance as records of a crucial moment in the development of religious reclusion imagery in waka. The author focuses on Saigyō, demonstrating how he created a new poetic space marked by a deepening of the tropes of sōan and yamazato, yielding a previously unarticulated realm of expression for his rigorous ideal of mountain seclusion. As “grass huts” and “mountain homes” became more commonly associated with hermits monks such as Saigyō, many of whom in fact spent the majority of their lives in the remote and indigent circumstances of mountain reclusion, the imagery relating to these spaces both shifted and expanded. Saigyō was a key figure in this development in Japanese poetics, and his yama fukami poems played an important role in the deepening and expansion of these topoi in the medieval period
反応性代謝物および免疫・炎症関連因子を考慮したフェニトイン誘導性肝障害発症機序に関する研究
No full text金沢大学博士（創薬科学）博士論文本文Full 以下に掲載：1.Toxicological Sciences 136(1) pp.250-263 2013. Oxford journal. 共著者：Sasaki E, Matsuo K, Iida A, Tsuneyama K, Fukami T, Nakajima M, Yokoi T 2.Toxicology Letters 232(1) pp.79-88 2015. Elsevier. 共著者：Sasaki E, Iwamura A, Tsuneyama K, Fukami T, Nakajima M, Kume T, Yokoi Tdoctoral thesi
反応性代謝物および免疫・炎症関連因子を考慮したフェニトイン誘導性肝障害発症機序に関する研究
No full text博士論文本文Full 以下に掲載：1.Toxicological Sciences 136(1) pp.250-263 2013. Oxford journal. 共著者：Sasaki E, Matsuo K, Iida A, Tsuneyama K, Fukami T, Nakajima M, Yokoi T 2.Toxicology Letters 232(1) pp.79-88 2015. Elsevier. 共著者：Sasaki E, Iwamura A, Tsuneyama K, Fukami T, Nakajima M, Kume T, Yokoi
Crystal Structure of KNa(SeO) at 340 K
No full textThe crystal structure of tripotassium sodium diselenate, KNa(SeO), at 340 K is studied by single-crystal X-ray diffraction. The space group symmetry (trigonal P3̅) and structure parameters are determined. It is found that there exists the difference of the direction and the magnitude of the atomic displacement at the two phase transitions of 346 K and 334 K
Studies on the chemical composition of a cyanophyte, Spirulina platensis
No full textThe chemical composition of Spirulina platensis NIES-46 and K-2 strains grown under varios condition of hight intensity and temperature were determined. The dry weight, chla, carotenoid, carbohydrate, protein, and lipid content in INES-46 strain were affected by various light intensities.
Changes in carbohydrate, protein, and pigment content in INES-46 and K-2 strains occurred with different temperature condition, with the growth yield of NIES-46 strain increasing significantly with increased protein content.
S. platensis NIES-46 and K2 strains contained sufficient quantities of essential amino acid except for methionine and histidine. The increase in the content of 18:2 and 18:2n6 fatty acids with respect to changes in light intensity and temperature would be important in maximizing their in S. platensis
Effect of saccharide additives on dehydration–drying kinetics and quality properties of dried kiwi fruit products
No full text[EN] The effects of saccharide additives on the dehydration and drying properties as well as the quality properties of dried kiwi fruit products were investigated. Sliced kiwi fruits were soaked and dehydrated in citric acid, glucose, sucrose and the pH-adjusted sugar solutions, individually. Osmotic dehydration and drying kinetic parameters were calculated using exponential models. Drying rate constants and water activities of dried kiwi fruits with osmotic dehydration were superior to those without osmotic dehydration. Soaking solutions with a lower pH led to a decrease in lightness. However, soaking sokution pH had no significant effect on the water activity or drying kinetics.Ueno, S.; Iijima, R.; Harada, M.; Liu, H.; Shimada, R.; Fukami, K. (2018). Effect of saccharide additives on dehydration–drying kinetics and quality properties of dried kiwi fruit products. En IDS 2018. 21st International Drying Symposium Proceedings. Editorial Universitat Politècnica de València. 851-856. https://doi.org/10.4995/IDS2018.2018.7487OCS85185
Attenuation of propagating spin wave induced by layered nanostructures
No full textSpin wave attenuation in the layered left perpendicularFeNi/Ptright perpendicular(6)/FeNi thin films was investigated by the time-domain electrical measurement. The spin-wave waveform was detected with an asymmetric coplanar strip transmission line, as an induced voltage flowing into a fast oscilloscope. We report that the amplitude of a spin-wave packet was systematically changed by controlling the thickness of a platinum layer, up to a maximum change of 50%. The virtues of spin wave, ultrafast propagation velocity and non-reciprocal emission, are preserved in this manner. This means that the Pt layer can manipulate an arbitral power-level of spin-wave input signal (reliable attenuator)
Perirenal fat stranding is not a powerful diagnostic tool for acute pyelonephritis [Corrigendum]
No full textFukami H, Takeuchi Y, Kagaya S, et al. Int J Gen Med. 2017;10:137–144.Page 137, Abstract, Results, the text “The frequency of PFS was 72% in the pyelonephritis group vs 39% in the control group” should read “The frequency of PFS was 72% in the pyelonephritis group vs 29% in the control group”.Page 140, Table 1, Control (PRB) column, last row, the data “93 (39%)” should read “93 (29%)”.Page 141, Discussion, line 5, the text “PFS was found in 39% of patients” should read “PFS was found in 72% of patients”. Read the original articl
Idiops pirassununguensis Fukami & Lucas 2005
No full textIdiops pirassununguensis Fukami & Lucas, 2005 Figs 2E–F, 4C, 6G, 27–28 Idiops pirassununguensis Fukami & Lucas, 2005: 2, figs 1–5. Diagnosis Males of Idiops pirassununguensis differ from those of other Neotropical species by having the tibial apophysis with a robust apical branch and a prominent spine in prolateral view (Figs 27H–I, 28C), metatarsus I long with a prolateral projection on the apical half (Fig. 27I) and the embolus with a prominent lamella that expands along the apical half, with a constriction near the sperm duct opening (Figs 27D–F, 28E–F). Females differ by having the spermathecae with long vertical ducts, and strong sclerotization on the transition between duct and receptacula, with the same diameter as ducts (Fig. 27L). Type material Holotype BRAZIL – São Paulo • ♂; Pirassununga; Nov. 1997; P. Valdujo leg.; IBSP 9565. Other material examined BRAZIL – Amapá • 7 ♂♂; Mazagão; 0°6′54″ S, 51°17′20″ W; 3 Dec. 2003; R.A. da Silva leg.; MCTP 16519. – Amazonas • 2 ♂♂; Manaus, PAE Lago Grande; 3°3′45″ S, 59°59′19″ W; 2–7 Jul. 2007; Projeto Geoma 2 leg.; INPA 6914, INPA 6916 • 1 ♀; Manaus, PAE Lago Grande; 5 May 2007; A.L. Tourinho and R. Saturnino leg.; INPA 6915. – Pará • 1 ♂; Parauapebas; 6°4′4″ S, 49°54′7″ W; Sep. 2008; C.A.R. Souza leg.; IBSP 225254. – Rondônia • 1 ♂; Pimenta Bueno; 11°40′21″ S, 61°11′37″ W; Jul. 2000; L. Carvalho leg.; IBSP 13335 • 2 ♂♂; same collection data as for preceding; IBSP 13337 • 4 ♂♂; Vilhena; 12°44′26″ S, 60°8′45″ W; Sep. 1999; L. Carvalho leg.; IBSP 14400 • 2 ♂♂; same collection data as for preceding; IBSP 14401, IBSP 14403 • 2 ♂♂; same collection data as for preceding; IBSP 14402. – Maranhão • 1 ♂; Caxias, Área de Proteção Ambiental Municipal do Inhamum; 04°53′30′′ S, 43°24′53″ W; 2–5 Oct. 2007; Lima-Lobato leg.; IBSP 130947 • 1 ♂; same collection data as for preceding; IBSP 129120. – Goiás • 2 ♂♂ (SEM); Caldas Novas, UHE Corumbá; 17°42′50″ S, 48°32′22″ W; 12– 23 Aug. 1996; M.T.I. Rodrigues et al. leg.; MZSP 15651. – Piauí • 5 ♂♂; Alvorada do Gurguéia, Fazenda Escola UFPI; 8°22′11.5″ S, 43°51′30.2″ W; 4–12 Sep. 2018; D.B.S. Barbosa et al. leg.; CHNUFPI 2715 • 5 ♂♂; same collection data as for preceding; CHNUFPI 2713 • 12 ♂♂; same collection data as for preceding; CHNUFPI 2714 • 15 ♂♂; same collection data as for preceding; CHNUFPI 2576 to 2590 • 2 ♂♂; Castelo do Piauí, Fazenda Bonito, ECB Rochas Ornamentais do Brasil LTDA; 5°13′59″ S, 41°41′14.5″ W; 11 Dec, 2005; F.M. Oliveira-Neto et al. leg.; MPEG 2336 • 2 ♂♂; same collection data as for preceding; MPEG 2339, MPEG 2401 • 1 ♂; Castelo do Piauí, Fazenda Bonito, ECB Rochas Ornamentais do Brasil LTDA; 5º13′47.7″ S, 41°41′36.6″ W; MPEG 2337 • 1 ♂; Castelo do Piauí, Fazenda Bonito, ECB Rochas Ornamentais do Brasil LTDA; 5º14′12.8″ S, 41°41′0.8″ W; MPEG 2338 • 2 ♂♂; Castelo do Piauí, Fazenda Bonito, ECB Rochas Ornamentais do Brasil LTDA; 5°19′19″ S, 41°33′10″; CHNUFPI CASTa0226 • 1 ♂; same collection data as for preceding; CHNUFPI CASTa0225 • 1 ♂; Castelo do Piauí, Fazenda Bonito, ECB Rochas Ornamentais do Brasil LTDA; 5°13′46.7″ S, 41°41′29.9″ W; 25 Oct. 2005; F.M. Oliveira-Neto et al. leg.; CHNUFPI 4024 • 2 ♂♂; Castelo do Piauí, Fazenda Bonito, ECB Rochas Ornamentais do Brasil LTDA; 5°13′59″ S, 41°41′14.5″ W; 8 May 2005; F.M. Oliveira-Neto et al. leg.; CHNUFPI 4028 • 2 ♂♂; same collection data as for preceding; CHNUFPI 2712 • 3 ♂♂; same collection data as for preceding; CHNUFPI 4025 to 4027 • 1 ♀; Guaribas, Parque Nacional da Serra das Confusões; 9°13′16″ S, 43°29′21″ W; Oct. 2006; P.R.R.Silva et. al. leg.; CHNUFPI 4029 • 1 ♂; same collection data as for preceding; CHNUFPI 4030 • 2 ♂♂; Gilbués, Olhos Dágua da Santa, PN Serra das Confusões; MZSP 19974 • 1 ♂; São Raimundo Nonato, Parque Nacional Serra da Capivara; 8°25′0″ S, 42°20′0″ W; 27 Oct.–9 Nov. 2012; R. Recoder and M. Teixeira Jr. leg.; IBSP 167608 • 1 ♂; Uruçuí, Vale do Rio Pratinha, 40 km from Uruçuí; 7°50′08″ S, 44°27′05″ W; M.P.D. Santos et al. leg.; CHNUFPI 2711 • 1 ♂; Uruçuí; Fazenda União, Topo da Chapada, 40 km from Uruçuí; 7°41′41″ S, 44°26′30″ W; 26 Oct. 2007; F.M. Oliveira-Neto leg.; CHNUFPI 2710. – Paraíba • 5 ♂♂; São José dos Cordeiros, RPPN Almas; 7°28′45″ S, 36°54′18″ W; 2008–2009; A. Vasconcelos leg.; IBSP 228197, IBSP 228200 to 228201, IBSP 228205, IBSP 228207 • 2 ♂♂; same collection data as for preceding; IBSP 228202 • 2 ♂♂; same collection data as for preceding; IBSP 228199 • 3 ♂♂; same collection data as for preceding; IBSP 228193. – Sergipe • 1 ♂; Canindé do São Francisco, Fazenda São José-Olhos Dʼágua, UHE de Xingó; 9°37′25″ S, 37°47′54″ W; 31 Oct. 2000; L. Ianuzzi leg.; IBSP 10183. – Mato Grosso • 2 ♂♂; Pontes e Lacerda; 15°19′10.38″ S, 59°17′33.70″ W; 5–10 Oct. 2013; R.A.K. Ribeiro leg.; UFMT • 2 ♂♂; Vila Bela da Santíssima Trindade, Córrego Areias; 14°50′29.9″ S, 69°39′01.2″ W; 2–4 Nov. 2013; R.A.K. Ribeiro leg.; UFMT • 1 ♂; Lucas do Rio Verde, PCH Canoa Quebrada; 12°47′ S, 56°00′ W; V.Azaias leg.; UFMT. – Minas Gerais • 1 ♂; Santana do Riacho, Parque Nacional Serra do Cipó; 19°22′1″ S, 43°32′17″ W; Oct. 2004; UFMG 1740 • 2 ♂♂; Santa Luzia; 19°46′12″ S, 43°51′3″ W; 9 Oct. 2004; UFMG 1730, UFMG 1732 • 1 ♂; Santa Luzia, 19º46′43.52″ S, 43º50′26.56″ W; 17 Sep. 2011; B.R.N. Leg keys; UFMG 8471 • 1 ♀; Morro do Pilar; 19º12′56″ S, 43º22′34″ W; 6 Aug. 2011; P.H. Martins leg.; UFMG 11296 • 1 ♀; Morro do Pilar; 19º12′56″ S, 43º22′34″ W; 29 Jul. 2011; P.H. Martins leg.; UFMG 11297 • 1 ♀; Santana do Riacho; 19º10′8″ S, 43º42′57″ W; 9 Jul. 2011; P.H. Martins leg.; UFMG 11264 • 2 ♂♂, 1 ♀; Santana do Riacho, Parque Nacional Serra do Cipó; 19°21′06.8″ S, 43°36′38.8″ W; 17–21 Oct. 2018; R.F. Ferreira, A. Galleti, P.H. Martins and V. Ghirotto leg.; CAD 833 • 1 ♀; Diamantina, Campus JK; 18°14′56″ S, 43°36′0″ W; 7 Mar. 2010; W.F. Silva leg.; CAD 27 • 1 ♂; São Gonçalo do Rio Preto, Parque Estadual do Rio Preto; 18°10′29.97″ S, 43°20′41.25″ W; 20–25 Oct. 2010; G. Monteiro, F. Sá, W.F. Silva and J.P.L. Guadanucci leg.; CAD 275 • 1 ♀; São Gonçalo do Rio Preto, Parque Estadual do Rio Preto; 14 Jan. 2010; J.P.L. Guadanucci, W.F. Silva, D. Moura, R. F. Ferreira and D. Weinmann leg.; CAD 14 • 1 ♂; Belo Horizonte, Campus UFMG; 19°52′19″ S, 43°57′58″ W; 27 Nov. 1999; E.S.S. Álvares leg.; UFMG 606 • 2 ♂♂; Belo Horizonte, Campus UFMG; 10–25 Sep. 2000; E.S.S. Álvares leg.; UFMG 607 • 1 ♂; Belo Horizonte, Campus UFMG; 30 Aug. 2000; E.S.S. Álvares leg.; UFMG 608 • 2 ♂♂; Belo Horizonte, Campus UFMG; 26 Sep. 2009; I.L.F. Magalhães leg.; UFMG 3211 • 1 ♂; Belo Horizonte, Reserva Biológica UFMG; IBSP 14488 • 1 ♂; Belo Horizonte, Campus UFMG, Centro Pedagógico UFMG; Jul. 2002; C. Torres leg.; IBSP 13659. – São Paulo • 1 ♂; Araraquara; 21°47′38″ S, 48°10′33″ W; 30 Sep. 1988; IBSP 9562 • 1 ♂; Ribeirão Preto; 21°10′40″ S, 47°48′36″ W; 24 Nov. 2004; IBSP 12337 • 1 ♂; Santo Antônio do Aracanguá; 20°56′13″ S, 50°29′45″ W; IBSP 14314 • 1 ♂; São Paulo, Parque dos Príncipes; 23°25′ S, 46°37′ W; IBSP 13324 • 1 ♂; Rio Pequeno; 23°33′ S, 46°43′ W; IBSP 3346 • 1 ♂; São Roque; 23°31′44″ S, 47°08′06″ W; 2 Aug. 2011; A.B. Canute leg.; IBSP 9133. Emended description Male and female recently described by Fukami & Lucas (2005). New data on the male and female are included here: Male (UFMG 607) HABITUS. See Fig. 27A. COLOR. Carapace with dark brown spots on anterior half, mainly on cephalic area near eyes (Fig. 27A). PROSOMA. Carapace and ocular arrangement as shown in Fig. 27A. Eye tubercle: 0.6 long; 1 wide. AME-ALE distance 0.8. Basal segment of chelicerae with a prolateral row of 6 large teeth and 4 small retrolateral teeth, grouped in basal half (Fig. 28A); rastellum presenting 12 spines of same size (Fig. 28B). LEGS. Tibia, metatarsus and tarsus I as shown in Fig. 27G. Pseudoscopula: on tarsus I divided into rows of strong setae; tarsus II–IV totally covered. PALP. Tibia with expansion of basal half of retrolateral depression (Figs 27C, 28D). Palpal bulb with short embolus with a strongly inclined basal portion and incomplete subapical torsion (Fig. 27D–F). SPINATION. Palp: Ti r24, Ta d0-0-4. Leg I: Fe d1-1-2, Ti v1r-1r-2r, Mt 1r-2r-1, Ta r2-1-0. Leg II: Fe d1- 1-2, Ti v0-2r-1r-1, Mt v1r-1r-1r-2, Ta p0-0-1, r2-2-0. Leg III: Fe d1-1-1, Pa d1-1-0, p3-5-8, r0-0-2, Ti v0-1r-0-2, p1-1-4, r1-1-2, Mt v3-4-0-4, p3- 2-2, r0-1-2, Ta p0-4-8, r0-4-8. Leg IV: Fe d1-1-2, Pa p16-3-0, Ti v1-2-2, Mt v1-2 -1-3, Ta p2-3-6, r0-0-2. Female (UFMG 11264) HABITUS. See Fig. 27J. MEASUREMENTS. TBL 19.1, CL 9.8, CW 8.8, LL 1.5, LW 1.8, SL 5.4, SW 4.8. COLOR. Similar to that of male, except sternum and coxae brownish and dark gray abdomen (Fig. 27J–K). PROSOMA. Carapace and ocular arrangement as shown in Fig. 27J. Eye tubercle: 2.2 long; 1.9 wide. AME-ALE distance 1.4. Eye diameters: AME 0.4, ALE 0.5, PME 0.3, PLE 0.6. Thoracic fovea procurved (Fig. 27J). Labium with 6 cuspules (Fig. 27K). Maxilla with 92 cuspules, distributed over anterior ventral half, with 15 large cuspules at anterior prolateral end and 18 large cuspules at anterior retrolateral end (Fig. 27K). Basal segment of chelicerae with a prolateral row of 6 large teeth and a retrolateral row with 3 small teeth, grouped in basal third, rastellum presenting 22–24 spines of same size (Fig. 27K). PALP AND LEG MEASUREMENTS. Palp = 14.4 (4.8, 3.1, 3.2, 3.3), I = 16.4 (5.3, 3.6, 3.6, 2.6, 1.3), II = 14.7 (4.8, 3.4, 2.9, 2.4, 1.2), III = 15.1 (4.1, 3.5, 2.4, 3.1, 2), IV = 20.3 (5.4, 4.1, 4.4, 4.2, 2.2). SPINATION. Palp: Ti p5-12-13, r7-12-14, Ta v0-0-3, p12-10-8, r12-14-8. Leg I: Ti p5-7-8, r7-8-12, Mt p12-9-11, r11-10-9, Ta v0-0-6, p5-4-2, r5-4-2. Leg II: Ti p3-6-6, r1-3-3, Mt p12-9-12, r5-5-6, Ta v0-0-5, p7-4-3, r6-3-1. Leg III: Pa p3-4-8, r0-0-3, Ti v1-1-1, p2-3-6, r0-2-4, Mt v2-2-2, p6-2-2, r8-1-2, Ta v0-6- 15. Leg IV: Pa p37-4-0, Ti v1-1-2, Mt v2-2-2, Ta v2-4-13. SPERMATHECAE. Ducts with non-sclerotized basal region, thicker than apical portion; sclerotized apical portion; spherical and non-sclerotized receptacula, with evident granules (Fig. 27L). Distribution Brazil. Widely distributed, with areas in the phytophysiognomies of the Amazon, Caatinga and Cerrado. Records for Central-West region (Distrito Federal and Goiás), North region (Amazonas, Amapá, Pará and Rondônia), Northeast region (Bahia, Maranhão, Paraíba, Piauí and Sergipe) and Southeast region (Minas Gerais and São Paulo) (Fig. 4C).Published as part of Fonseca-Ferreira, Rafael, Guadanucci, José Paulo Leite, Yamamoto, Flávio Uemori & Brescovit, Antonio Domingos, 2021, Taxonomic revision of the Neotropical spiders of the genus Idiops Perty, 1833 (Araneae, Idiopidae), with description of four new species, pp. 1-71 in European Journal of Taxonomy 780 (1) on pages 50-54, DOI: 10.5852/ejt.2021.780.1581, http://zenodo.org/record/576128
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