211 research outputs found
Fisetin inhibits human melanoma cell growth through direct binding to p70S6K and mTOR: findings from 3-D melanoma skin equivalents and computational modeling.
No full textTrichoderma erinaceum Bissett, Kubicek & Szackacs, Can. J. Bot.
No full text7. Trichoderma erinaceum Bissett, Kubicek & Szackacs, Can. J. Bot. 8: 8, 00. Figure 9 Section— Trichoderma Description: Colony: 7.0– 8.5 cm growth was observed in 4 days on PDA medium. White mycelium producing yellow pustules turning to dark green color with the age. The pustules uniformly spread throughout the plate leaving a concentric green ring at the center. The reverse of the plate is colorless. Conidiophores: The main axis terminates in a septate elongation with a single phialide at its tip and fertile branches arising near the base. Conidiophore branches arising at angles of 90° or less concerning the main axis, paired or not, rebranching sparingly to produce phialides directly or at the tips of short secondary branches. Phialides: Phialides arising from branches are solitary or in whorls of 2 or 3, straight, cylindrical, flask-shaped and swollen in the middle. Conidia: Ellipsoidal to broadly ellipsoidal, 4.00–5.50 × 3.5–4.5 µm, green. Chlamydospores: Chlamydospores terminal to intercalary, globose to subglobose, 8.00–13.00 µm. Cultures examined: ITCC 7287 (Withania somnifera rhizosphere, Delhi); ITCC 7288 (Groundnut rhizosphere, Tirupati, AP); ITCC 7289 (Soil, SUKAST, Jammu, J&K). Diagnostic features: Conidiophores arising at 90° and ends with a single phialide with the fertile branches at the base and produce solitary and flask-shaped phialides directly on secondary branches. Ecology and habitats: Soil, endophyte of woody tissue of Theobroma cacao in Peru.Published as part of Thokala, Prameeladevi, Narayanasamy, Prabhakaran, Kamil, Deeba & Choudhary, Shiv Pratap, 2021, Polyphasic taxonomy of Indian Trichoderma species, pp. 1-27 in Phytotaxa 502 (1) on page 11, DOI: 10.11646/phytotaxa.502.1.1, http://zenodo.org/record/542489
Trichoderma ghanense Doi, Abe & J. Sugiyama, Bull. Natl. Sci. Mus. Ser. B (Bot.
No full text8. Trichoderma ghanense Doi, Abe & J. Sugiyama, Bull. Natl. Sci. Mus. Ser. B (Bot.):, 987. Figure 0 Section—Longibrachiatum Description: Colony: 7 – 8.5 cm in four days. Aerial hyphae cottony grown throughout the plate. Artemisia green white cushionlike small pustules formed throughout the plate but concentrating more than 2 cm away from the edge of the plate. The white pustules turned to green after conidiation. Reverse of the plate was colorless. Conidiophores: Conidiophores typically consisting of a strongly developed central axis from which arise, toward the tip, solitary phialides and further from the tip progressively longer, often paired, secondary branches. Phialides arising directly from secondary branches, typically not in whorls. Phialides: Phialides cylindrical or straight or hooked. Conidia: Conidia green, ellipsoidal, 6.0–7.5× 4.0–4.5 µm and smooth. FIGURE 0. Trichoderma ghanense ( A ) Growth on PDA, ( B ) Pustules with ooze, ( C ) Reverse of the plate, ( D ) Conidiophore branching, ( E,F,G ) Phialide disposition, ( H ) Spores, ( I ) Chlamydospores. Chlamydospores: Chlamydospores abundant, globose, 7.0–12.0 µm in dia. and smooth. Culture Examined: ITCC 7279 (Soil, Arunachal Pradesh). Diagnostic features: Greenish white cushion-like small pustules, Conidiophore bearing cylindrical or straight or looked solitary phialides. Chlamydospores are abundant and globose. Ecology and habitats: Soil. FIGURE. Trichoderma harzianum ( A ) Growth on PDA, ( B ) Pustules, ( C ) Reverse of the plate; ( D, E ) Conidiophore branching, ( F,G ) ampulliform phialides, ( H ) Spores, ( I ) Spores under SEM, ( J ) Chlamydospores. FIGURE. Trichoderma hamatum ( A ) Growth on PDA, ( B ) Pustules, ( C ) Reverse of the plate, ( D ) pustule, ( E.F ) Conidiophore branching, ( G,H,I ) Phialide disposition, ( J ) Spores, ( K ) Chlamydospores.Published as part of Thokala, Prameeladevi, Narayanasamy, Prabhakaran, Kamil, Deeba & Choudhary, Shiv Pratap, 2021, Polyphasic taxonomy of Indian Trichoderma species, pp. 1-27 in Phytotaxa 502 (1) on pages 11-13, DOI: 10.11646/phytotaxa.502.1.1, http://zenodo.org/record/542489
Role of YB-1, a regulator of epithelial to mesenchymal transition, in prostate cancer: Inhibition by a dietary flavonoid fisetin
No full textFisetin targets YB-1/RSK axis independent of its effect on ERK signaling: insights from in vitro and in vivo melanoma models
No full textAbstract The anti-proliferative activity of dietary flavonoid fisetin has been validated in various cancer models. Establishing its precise mechanism of action has proved somewhat challenging given the multiplicity of its targets. We demonstrated that YB-1 promotes epithelial-to-mesenchymal transition and its inhibition suppressed tumor cell proliferation and invasion. The p90 ribosomal S6 kinase (RSK), an important ERK effector, activates YB-1 to drive melanoma growth. We found that fisetin treatment of monolayer/3-D melanoma cultures resulted in YB-1 dephosphorylation and reduced transcript levels. In parallel, fisetin suppressed mesenchymal markers and matrix-metalloproteinases in melanoma cells. Data from cell-free/cell-based systems indicated that fisetin inhibited RSK activity through binding to the kinase. Affinity studies for RSK isoforms evaluated stronger interaction for RSK2 than RSK1. Competition assays performed to monitor binding responses revealed that YB-1 and RSK2 do not compete, rather binding of fisetin to RSK2 promotes its binding to YB-1. Fisetin suppressed YB-1/RSK signaling independent of its effect on ERK, and reduced MDR1 levels. Comparable efficacy of fisetin and vemurafenib for inhibiting melanoma growth was noted albeit through divergent modulation of ERK. Our studies provide insight into additional modes of regulation through which fisetin interferes with melanoma growth underscoring its potential therapeutic efficacy in disease progression
Pomegranate juice consumption reduces simulated ischemic stroke damage and increases brain antioxidant status in rats
No full textPomegranate phytochemicals / Navindra P. Seeram ... [et al.] -- Antioxidative properties of pomegranate : in vitro studies / Mira Rosenblat and Michael Aviram -- Bioavailability of pomegranate polyphenols / Francisco A. Tom?s-Barber?n, Navindra P. Seeram, and Juan Carlos Esp?n -- Protection against cardiovascular disease / Bianca Fuhrman and Michael Aviram -- Protection against stroke / Marva I. Sweeney-Nixon -- Anticancer potential of pomegranate / Shishir Shishodia ... [et al.] -- Molecular mechanisms of chemoprevention of cancer by pomegranate / Deeba Syed ... [et al.] -- Pomegranate and prostate cancer chemoprevention / John T. Leppert and Allan J. Pantuck -- Assessment of estrogenicity of pomegranate in an in vitro bioassay / Diane M. Harris, Emily Besselink, and Navindra P. Seeram -- Absence of significant estrogenic effects in the postmenopausal population / Michelle P. Warren ... [et al.] -- Antimicrobial activities of pomegranate / G.K. Jayaprakasha, P.S. Negi, B.S. Jena -- Commercialization of pomegranates : fresh fruit, beverages, and botanical extracts / Navindra P. Seeram, Yanjun Zhang, and David Heber -- Pomegranates: a botanical perspective / David W. Still -- Postharvest biology and technology of pomegranates / Adel A. Kader
Abstract 1179: Unique metabolic profile of Vemurafenib-resistant melanoma cells: a quantitative proteomics approach
No full textAbstract:
Malignant melanoma is noted for its aggressive clinical behavior and propensity for lethal metastasis. Despite the remarkable success of BRAF inhibitor Vemurafenib in clinics, emergence of resistance remains a limiting factor in increasing overall survival. Thus, comprehensive studies are needed to increase the biological understanding of BRAF-resistant tumors in order to develop effective therapeutic regimens. To study the molecular mechanism(s) involved in acquired resistance, we generated a Vemurafenib-resistant cell line in the BRAFV600E mutated SK-Mel28 melanoma cell background. Incubation of SK-Mel28 cells with Vemurafenib (0.1 μM) resulted in an initial loss of cell viability after which majority of cells resumed proliferation. Thereafter, cells were exposed to gradually increasing Vemurafenib concentrations and allowed to proliferate in the presence of 5 μM Vemurafenib, to generate the A2-1b resistant cell line. Viability assays established the IC50 of parental SK-Mel28 cells as 0.2 μM, while IC50 > than 12 μM was noted for the Vemurafenib-resistant A2-1b population. We utilized a quantitative proteomics strategy, employing label-free nano-LC-MS/MS technology on a Q-exactive, to compare the proteome of A2-1b with SK-Mel28 cells. The data searched against the human proteome using the Sequest search engine and further analyzed with SIEVE software resulted in the positive identification of 1720 proteins (≤1% FDR). This data set containing proteins with only uniquely identified peptides with a high level of confidence (p<0.05) was uploaded into Ingenuity Pathway Analysis and Panther softwares, with number of peptides and corresponding ratio between the two groups, set as observational parameters. Notably, the largest fraction (49%) of identified proteins belonged to cellular metabolic processes. Glycolysis, gluconeogenesis and NADH repair were the major pathways modulated in Vemurafenib-resistant A2-1b cells. In addition, TCA cycle and unfolded protein response was significantly affected. A subset of 13 proteins, linked to these pathways and not previously associated with acquired resistance was identified. The upregulated proteins included CALD1, OR4A15, PTPRK, CALU, IDH3A, RNH1, CLIC4, SPAG17, SND1 and VCP while ENO2, TPI1 and GAPDH were significantly downregulated. In summary, the proteomic approach applied for the first time to study acquired resistance to BRAF inhibition identified novel targets which upon further validation in appropriate models has the potential to provide valuable therapeutic strategies against Vemurafenib-resistant melanomas.
Citation Format: Deeba N. Syed, Rahul K. Lall, Iram Majeed, Feng Liu, Frank L. Meyskens, Hasan Mukhtar. Unique metabolic profile of Vemurafenib-resistant melanoma cells: a quantitative proteomics approach. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 1179. doi:10.1158/1538-7445.AM2015-117
Trichoderma tomentosum Bissett, Can. J. Bot.
No full text9. <i>Trichoderma tomentosum</i> Bissett, Can. J. Bot. 69:, 99. Figure <p>Section— Trichoderma</p> <p>Description:</p> <p>Colony: 7–7.5 cm growth was observed in four days. White mycelium growing mostly at the edge of the petriplate with white small pustules. Reverse of the plate was colourless. Pustules scattered around the periphery of the Petri dish, yellow-green or gray-green, uniformly cottony.pustules pulvinate and conspicuously hairy, very dense. Fertile extensions of conidiophores not formed. Sterile hairs spiraled, branched, thin-walled, septate, and acute at the tip.</p> <p>Conidiophores: Conidiophores comprising an branched and unbranched sterile hair from the base of which arise at right angles short, broad lateral branches, each comprising a 3–4 cells, rebranching at right angles to produce secondary fertile branches of 1–2 cells with phialides arising from the primary and secondary branches in clusters.</p> <p>Phialides: Phialides are ampulliform, short and broad, almost ovoidal with a distinct neck, forming in dense, grape-like clusters at the tips of fertile branches.</p> <p> <b>Figure.</b> <i>Trichoderma tomentosum</i> <b>(</b> A <b>)</b> Growth on PDA, <b>(</b> B <b>)</b> Pustules, <b>(</b> C <b>)</b> Reverse of the plate, <b>(</b> D,E <b>)</b> Sterile hairs extending beyond pustules, <b>(</b> F,G,H <b>)</b> Conidiophores consisting of a sterile elongation with phialides clustered at the base, <b>(</b> I <b>)</b> Spores.</p> <p> <b>FIGURE.</b> <i>Trichoderma virens</i> <b>(</b> A,B <b>)</b> Growth on PDA, <b>(</b> C <b>)</b> Pustules, <b>(</b> D <b>)</b> Reverse of the plate, <b>(</b> E,F <b>)</b> Conidiophore branching, <b>(</b> G,H, I,J <b>)</b> Phialide disposition, <b>(</b> K <b>)</b> Spores, <b>(</b> L <b>)</b> Spores under SEM, <b>(</b> M <b>)</b> Chlamydospores.</p> <p>Conidia: Conidia green, broadly ellipsoidal, 3.0–5.0× 2.0–3.5 µm, smooth.</p> <p>Culture Examined: ITCC 7269 (Soil, Kochi, Kerala).</p> <p>Diagnostic features: Trichoderma tomentosum can be distinguished by the small conidia, short phialides and conidiophores apical elongations that are sterile, long and flexuous.</p> <p>Ecology and habitats: Soil.</p>Published as part of <i>Thokala, Prameeladevi, Narayanasamy, Prabhakaran, Kamil, Deeba & Choudhary, Shiv Pratap, 2021, Polyphasic taxonomy of Indian Trichoderma species, pp. 1-27 in Phytotaxa 502 (1)</i> on pages 21-23, DOI: 10.11646/phytotaxa.502.1.1, <a href="http://zenodo.org/record/5424892">http://zenodo.org/record/5424892</a>
Study of some connectivity index of subdivided mk graphs of ladder and triangular ladder graph
No full textAn m(k)-graph of a graph G is defined by taking m >= 2 copies G(v), ..., G(m) of a graph G in which every vertex u(h) of copy G(h) is adjacent to a corresponding vertex v(m) of copy G(m). An m(k)-graph is represented by m(k)(G). In this paper we work on some degree based connectivity indices of subdivided m(k)-graph generated by ladder and triangular ladder graph. Also closed formulas for computing various degree based topological indices of said families of graphs have been presented
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