23,819 research outputs found
Depolarization and decreased surface expression of K+ channels contribute to NSAID-inhibition of intestinal restitution
No full textNon-steroidal anti-inflammatory drugs (NSAIDs) contribute to gastrointestinal ulcer formation by inhibiting epithelial cell migration and mucosal restitution; however, the drug-affected signaling pathways are poorly defined. We investigated whether NSAID inhibition of intestinal epithelial migration is associated with depletion of intracellular polyamines, depolarization of membrane potential (Em) and altered surface expression of K+ channels. Epithelial cell migration in response to the wounding of confluent IEC-6 and IEC-Cdx2 monolayers was reduced by indomethacin (100μM), phenylbutazone (100μM) and NS-398 (100μM) but not by SC-560 (1μM). NSAID-inhibition of intestinal cell migration was not associated with depletion of intracellular polyamines. Treatment of IEC-6 and IEC-Cdx2 cells with indomethacin, phenylbutazone and NS-398 induced significant depolarization of Em, whereas treatment with SC-560 had no effect on Em. The Em of IEC-Cdx2 cells was: −38.5±1.8mV under control conditions; −35.9±1.6mV after treatment with SC-560; −18.8±1.2mV after treatment with indomethacin; and −23.7±1.4mV after treatment with NS-398. Whereas SC-560 had no significant effects on the total cellular expression of Kv1.4 channel protein, indomethacin and NS-398 decreased not only the total cellular expression of Kv1.4, but also the cell surface expression of both Kv1.4 and Kv1.6 channel subunits in IEC-Cdx2. Both Kv1.4 and Kv1.6 channel proteins were immunoprecipitated by Kv1.4 antibody from IEC-Cdx2 lysates, indicating that these subunits co-assemble to form heteromeric Kv channels. These results suggest that NSAID inhibition of epithelial cell migration is independent of polyamine-depletion, and is associated with depolarization of Em and decreased surface expression of heteromeric Kv1 channels.ID: S0006295207001931; M3: Article; Accession Number: S0006295207001931; Author: L.C. Freeman (b); Author: D.F. Narvaez (a); Author: A. McCoy (a); Author: F.B. von Stein (c); Author: S. Young (b); Author: K. Silver (a); Author: S. Ganta (b); Author: D. Koch (b); Author: R. Hunter (b); Author: R.F. Gilmour (c); Author: J.D. Lillich (a, ⁎); Affiliation: Department of Clinical Sciences, Kansas State University, Manhattan, KS 66506, United States; Affiliation: Department of Anatomy and Physiology, Kansas State University, Manhattan, KS 66506, United States; Affiliation: Department of Biomedical Sciences, Cornell University, Ithaca, NY 14853, United States; Keyword: Non-steroidal anti-inflammatory drugs; Keyword: Intestinal epithelial cells; Keyword: Membrane potential; Keyword: Potassium channels; Number of Pages: 12; Language: English;Source type: Electronic(1)http://search.ebscohost.com/login.aspx?direct=true&db=edselp&AN=S0006295207001931&site=eds-live&scope=sit
Recommended from our members
Hamilton K. Redway Family papers, 1825-1916
No full textList of quartermaster's supplies transferred by John Freeman to Hamilton K. Redway at Camp Piatt, West Virginia
Hamilton K. Redway Family papers, 1825-1916
No full textList of quartermaster's supplies transferred by John Freeman to Hamilton K. Redway at Camp Piatt, West Virginia
Conformational rearrangements enable iterative backbone -methylation in RiPP biosynthesis
No full textR Code:
Supplementary Data 1. Code for running kinetics simulations
File name: Supplementary Data 1.R
Mass Spectrometry .raw files:
Supplementary Figure 4a-c. Mass spectrometric analysis of split borosin coexpressions.
File name: 20200110_FSM1167_SonA+SonM_24.5min.raw
Supplementary Figure 4d-j. Mass spectrometric analysis of split borosin coexpressions.
File name: 20200909_KC1063_StrA+StrM_15min_redalk_18pm4.raw
Supplementary Figure 16. SonM in vitro reactions analyzed by LC-MS/MS and
compared to kinetic model simulations.
File names:
20200220_FSM1178_kineticmodeling_t=2.raw
20200220_FSM1179_kineticmodeling_t=2.raw
20200220_FSM1180_kineticmodeling_t=8.raw
20200220_FSM1181_kineticmodeling_t=8.raw
20200220_FSM1182_kineticmodeling_t=20.raw
20200220_FSM1183_kineticmodeling_t=20.raw
20200220_FSM1184_kineticmodeling_t=40.raw
20200220_FSM1185_kineticmodeling_t=40.raw
20200220_FSM1186_kineticmodeling_t=60.raw
20200220_FSM1187_kineticmodeling_t=60.raw
Supplementary Figure 25a. Mass spectrometric analysis of SonM mutant in vitro
reactions.
File name: 20190701_kc1007_His-SonA_Y93F.raw
Supplementary Figure 25b. Mass spectrometric analysis of SonM mutant in vitro
reactions.
File name: 20190515_fsm1155_his-SonA-sonMT-R67K.raw
Supplementary Figure 25c. Mass spectrometric analysis of SonM mutant in vitro
reactions.
File name: 20190515_fsm1154_his-sonA_sonMTR67A.raw
Supplementary Figure 25d. Mass spectrometric analysis of SonM mutant in vitro
reactions.
File name: 20190701_kc1008_His-SonA_Y58F.raw
Supplementary Figure 25e. Mass spectrometric analysis of SonM mutant in vitro
reactions.
File name: 20190515_fsm1156_his-sonA-SonMT-Y71F.raw
Supplementary Figure 25f. Mass spectrometric analysis of SonM mutant in vitro
reactions.
File name: 20190701_kc1009_His-SonA_DBLMUT.rawThe data deposited here are raw files, R code and mass spec, associated with the results presented in the paper, "Conformational rearrangements enable iterative backbone N-methylation in RiPP biosynthesis." These data have been made publicly available in keeping with the journal's data availability policy.National Institutes of Health (R35 GM133475 to M.F.F.) and the University of Minnesota along with the BioTechnology Institute (M.F.F., M.H.E., W.A.H.)Miller, Fredarla S.; Crone, Kathryn K.; Jensen, Matthew R.; Shaw, Sudipta; Harcombe, William R.; Elias, Mikael H.; Freeman, Michael F.. (2021). Conformational rearrangements enable iterative backbone -methylation in RiPP biosynthesis. Retrieved from the University Digital Conservancy, https://doi.org/10.13020/y8ry-gm18
The management of spondylolysis and spondylolisthesis
No full textAbstract not availableBrian J. C. Freeman and Ujjwal K. Debnat
Regarding Constance Freeman interview
No full textGuest: Constance FreemanHost: Ken RockburnConstance J. Freeman, Regional Director of IDRC's East and Southern Africa Regional Office, discusses NEPA
Introducing Visual C# 2010
No full textIf you're new to C# programming, this book is the ideal way to get started. Respected author Adam Freeman guides you through the C# language by carefully building up your knowledge from fundamental concepts to advanced features. The book gradually builds up your knowledge, using the concepts you have already grasped to support those that come next. You will explore all the core areas of the C# language and the .NET Framework on which it runs. Particular attention is paid to the creation of Web and Windows applications and data access - danger zones where novice programmers often go awry in th
Hamilton K. Redway Family papers, 1825-1916
No full textList of quartermaster's stores transferred from John Freeman to Hamilton Redway at Camp Piatt, West Virginia on December 1, 1864
Recommended from our members
Hamilton K. Redway Family papers, 1825-1916
No full textList of quartermaster's stores transferred from John Freeman to Hamilton Redway at Camp Piatt, West Virginia on December 1, 1864
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