58,842 research outputs found
Do Han Jun
No full text학위논문(박사)아주대학교 일반대학원 :전자공학과,2012. 2This thesis presents a vlaveless thermopneumatic micropump using surface tension. This micropump uses the surface tension and capillary attraction to control the flow for simple structure without any moving part such as a membrane or valve. In this dissertation, the influences of geometries of the micro channel and micro pillar on the performance of the micropump are compared. Commercial finite volume method (FVM) simulation software is used to simulate micropump operations.
First, theories of microfluid mechanics are presented in chapter 2. And the valveless thermopneumatic micropump with simple structure is designed, fabricated, simulated and tested in this chapter. This micropump discharges the fluid using thermopneumatic pressure and fills the fluid using negative pressure and capillary attraction. And during the refill time the micro channel works like a valve using surface tension. Therefore the flow can be controlled without any additional components. This micropump operates at 3.5V for 4 seconds to discharge and its size is 11.7 x 8.8 x 0.7 cm3. And the height of micro structure is 80 ㎛.
Second, various micropump geometries are designed to improve the micropump performance especially to reduce the backward flow loss. In the chapter 3, the influences of the micropump geometries such as the channel direction angle and the expansion angle on the micropump performance are compared with experimental tests and FVM simulations. In this chapter, six different geometries of micropumps are designed with three different channel direction angles and two different expansion angles. For more accurate comparison, three geometries with the same effective pump chamber are additionally designed. In this chapter, micropump size is 11.7 x 8.8 x 1.7 cm3 and the height of the micro structure is 100 ㎛. And these pumps discharge 120 nL for 4 - 7seconds. The channel direction angle affects to the backward flow loss and the expansion angle affects to the refill time and backward flow loss.
Third, micro pillar structures are designed to improve the efficiency of the micropump operations especially to reduce the backward flow loss and discharge time. And the influences of geometries of the micro pillars on the micropump performance are compared with experimental tests and FVM simulations. Various micro pillar structures are designed with four different locations and five different sizes. Micro pillar structures increase the surface area and the surface tension. Therefore they reduce the backward flow loss. In this chapter, micropump size is 11.7 x 8.8 x 1.7 cm3 and the height of micro structure is 100 ㎛. And these pumps discharge 100 nL for 4 seconds. And locations and sizes of the micro pillar structures affect to the backward flow loss.ABSTRACT i
LIST OF FIGURES v
LIST OF TABLES xi
Chapter I. INTRODUCTION 1
1.1 Microfluidic devices 1
1.2 Review of micropumps 2
1.3 Research objectives 4
Chapter II. The VALVELESS THERMOPNEUMATIC MICROPUMP STRUCTURE 6
2.1 Characteristics of the microfluid 6
2.2 Theory to design the micro channel 7
2.1.1 Capillary force 8
2.1.2 Surface tension 9
2.1.3 Flow resistance 12
2.3 Valveless thermopneumatic micropump 13
2.3.1 Structure 13
2.3.2 Operation sequence 15
2.3.3 Simulation results 18
2.3.4 Fabrication process 20
2.3.5 Experimental results 24
2.4 Summary 28
Chapter III. THE INFLUENCE OF GEOMETRY ON THE MICROPUMP OPERATION 29
3.1 Design and operation 29
3.2 Structure 40
3.3 Simulation results 45
3.4 Fabrication 53
3.5 Experimental results 57
3.3 Summary 68
Chapter IV. THE INFLUENCE OF A MICROPILLAR ON THE MICROPUMP 69
4.1 Design 70
4.2.1 Design of principles 70
4.2.2 Design of micro pillar structure 74
4.2 Simulation results 78
4.3 Fabrication 86
4.4 Experimental results 89
4.5 Summary 101
Chapter V. Conclusions and Outlook 102
REFERENCES 104Maste
Synthesizing Service Composition Models on the Basis of Temporal Business Rules
No full textTransformational approaches to generating design and implementation models from requirements can bring effectiveness and quality to software development. In this paper we present a framework and associated techniques to generate the process model of a service composition from a set of temporal business rules. Dedicated techniques including path-finding, branching structure identification and parallel structure identification are used for semi-automatically synthesizing the process model from the semantics-equivalent Finite State Automata of the rules. These process models naturally satisfy the prescribed behavioral constraints of the rules. With the domain knowledge encoded in the temporal business rules, an executable service composition program, e.g., a BPEL program, can be further generated from the process models. A running example in the e-business domain is used for illustrating our approach throughout this paper. Electronic supplementary material The online version of this article (doi: 10.1007/s11390-008-9196-x) contains supplementary material, which is available to authorized users.Jian Yu, Yan-Bo Han, Jun Han, Yan Jin, Paolo Falcarin and Maurizio Morisi
Proto-oncogene c-jun expression is induced by AML1-ETO in a JNK dependent manner:possible role in the pathogenesis of acute myeloid leukemia
No full textOverexpression of proto-oncogene c-jun and constitutive activation of the Jun NH2-terminal kinase (JNK) signaling pathway have been implicated in the leukemic transformation process. However, c-jun expression has not been investigated in acute myeloid leukemia (AML) cells containing the most common chromosomal translocations. t(8;21) is one of the most common AML-associated translocation and results in the AML1-ETO fusion protein. Overexpression of AML1-ETO in NIH3T3 cells leads to increased phosphorylation of Ser63 in c-Jun, which is generally JNK dependent. The role of the JNK signaling pathway for the functional properties of AML1-ETO is, however, unknown.
In the present study we found high expression levels of c-jun mRNA in t(8;21), t(15;17) or inv(16) positive patient cells by microarray analysis. Within t(8;21) positive patient samples, there was a correlation between AML1-ETO and c-jun mRNA expression levels. In myeloid U937 cells, c-jun mRNA and c-Jun protein expression levels increased upon induction of AML1-ETO. AML1-ETO transactivated the human c-jun promoter through the proximal AP-1 site via activating the JNK signaling pathway. JNK targets c-Jun and ATF-2, which also bind to the proximal AP-1 site in U937 cells, were also phosphorylated upon AML1-ETO induction. Furthermore, AML1-ETO induction increased the DNA binding capacity of c-Jun and ATF-2 to the proximal AP-1 site of the c-jun promoter, which might result in their enhanced transactivation capacities.
Interference with JNK and c-Jun activation by using JIP-1 or a JNK inhibitor reduced the transactivation capacity of AML1-ETO on the c-jun promoter and the pro-apoptotic function of AML1-ETO in U937 cells. AML1-ETO seems to activate the JNK signaling pathway by inducing the expression of a cytoplasmic factor, possibly G-CSF, because supernatant of AML1-ETO expressing cells was sufficient to induce phosphorylation of JNK and c-Jun in wildtype U937 cells.
This data demonstrates a novel mechanism of how AML1-ETO might exert positive effects on target gene expression and identifies the proto-oncogene c-jun as a common target gene in AML patient cells.Überexpression des Proto-Onkogens c-jun und konstitutive Aktivierung des Jun NH2-terminalen Kinase (JNK)-Signaltransduktionsweges sind wichtig für die leukämische Transformation in der Chronischen Myeloischen Leukämie. Die Expression von c-jun bei Akuter Myeloischer Leukämie (AML) mit den häufigsten reziproken Translokationen ist jedoch unbekannt. Bei einer der häufigsten AML Translokation t(8;21) wurde in Fibroblastenzellen gezeigt, daß das AML1-ETO-Fusionsgen die Phosphorylierung des Serin 63 in c-Jun erhöht. Die Rolle des JNK-Signalweges, der c-Jun am Serin 63 phosphorylieren kann, für die Funktion von AML1-ETO wurde bisher jedoch nicht untersucht. Weiterhin kann aktiviertes c-Jun durch eine positive Rückkoppelungsschleife über den c-jun Promotor zur Erhöhung der c-Jun Expression führen.
In der vorliegenden Arbeit konnten wir zeigen, daß AML Patientenzellen mit den häufigen Translokationen: t(8;21), t(15;17) oder inv(16) mehr c-jun mRNA besitzen im Vergleich zu Knochenmarkszellen gesunder Probanden. Weiterhin fanden wir eine hohe Korrelation zwischen der AML1-ETO und der c-jun mRNA bei t(8;21) positiven Patientenzellen. Induktion von AML1-ETO in der myeloischen U937 Zellinie erhöhte sowohl c-jun mRNA als auch c-Jun Proteinexpression. Damit konnten wir zeigen, daß AML1-ETO die Erhöhung der c-jun Expression bewirkt. Wir untersuchten den molekularen Mechanismus in U937 Zellen mittels transienter Transfektionen und fanden, daß AML1-ETO den c-jun Promotor durch die proximale AP-1 Seite transaktiviert. Diese Transaktivierung erfolgte indirekt über Aktivierung des JNK-Signaltransduktionsweges durch AML1-ETO. AML1-ETO-Induktion führte auch zur Phosphorylierung der JNK-Zielproteine c-Jun und ATF-2. Diese konnten im Gelretardierungsassay an die proximale AP-1 Seite des c-jun Promotors binden und wurden durch AML1-ETO-Induktion in ihrer Bindungsfähigkeit verstärkt. Deshalb nehmen wir an, daß die Transaktivierungskapazität des c-jun Promotors durch AML1-ETO über die Aktivierung des JNK-Signalweges läuft
무증상성인에서 컴퓨터단층촬영을 이용한 고관절 cam변형의 빈도
No full textPURPOSE: Femoroacetabular impingement (FAI) which restricts range of hip motion is regarded as an important cause leading to early development of degenerative arthritis. Although three-dimensional imaging such as CT and MRI are regarded as precise imaging modalities for three dimensional morphology of FAI, the modalities have several limitations for screening tool in out-patient clinic. The paucity of morphologic data of FAI in Korean makes it difficult to choose which method of radiograph is the most useful to screen out general orthopedic problems. We postulated the distribution of cam deformity have individual variation in Korean population. Thus we investigated the distribution of cam deformity in asymptomatic Korean population.
MATERIALS AND METHODS: From Jan 2011 to Dec 2015, hip CT images of 120 subjects without any history around hip joint were evaluated. A computer program which reconstructs three dimensional model from CT scan was used to provide sectional images which cross the central axis of femoral head and neck. Alpha angle was measured in each sectional images. Alpha angle above 55 degrees were regarded as cam deformity.
RESULTS: Mean alpha angle was 43.5°(34.7-56.1°) in 3 o’clock, 51.24°(39.5-58.8°) in 2 o’clock, 52.45° (43.3-65.5°) in 1 o’clock, 44.09°(36.8-49.8°) in 12 o’clock, 40.71°(33.5-45.8°) in 11 o’clock, 39.21°(34.1-44.6°) in 10 o’clock. Alpha angle in 1 and 2 o’clock was significantly larger than any locations (P<0.01). The prevalence of cam deformity was 15% and 15.8% in 1 and 2 o’clock, respectively.
CONCLUSION: Cam deformity of FAI was observed on 25.8% of asymptomatic hip. The most common region of cam deformity was antero-superior area of femoral head-neck junction (1 and 2 o’clock). The present study first reported the prevalence and the distribution of cam deformity in asymptomatic Korean population. The results would provide anatomical comprehension of cam deformity for diagnosis and surgical treatments.목적: 대퇴비구 충돌(Femoroacetabular impingement: FAI)은 고관절의 운동 범위를 감소시키는 것은 물론 초기 관절염의 초래하는 중요한 원인으로 추정되고 있다. 단순 방사선 촬영 단독보다 CT 나 MRI 와 같은 단층 촬영이 더욱 정확한 진단적 방법으로 간주되지만 외래에서 매번 적용하기에는 제한이 따른다. 우리나라 성인의 FAI에 대한 단층 촬영의 데이터가 부족하여 저자들은 한국인에서 cam 형 충돌의 위치가 개인마다 다를 것으로 가설을 수립하였다. 이에 컴퓨터 단층촬영을 이용하여 무증상성인의 cam 형 변형의 빈도를 측정하고자 한다.
대상 및 방법: 2011년 1월부터 2015년 12월까지 본원에서 120개 고관절에 특이병력 없는 무증상 성인 120명의 고관절 CT영상을 이용하였다. CT영상을 INFINITT Xelis 프로그램을 이용하여 골 두-경부 중심점을 잇는 선을 중심으로 근위 대퇴부를 시계 시스템을 이용하여 3차원적으로 분할하여 각 구간에서 알파각도를 측정하였다. 알파각도는 55도 보다 큰 값이면 고관절 Cam형 충돌로 판정하였다.
결과: 3시부터 10시(대퇴골 전방에서 후방)방향으로의 평균 알파각은 3시에서 43.5°(34.7-56.1°), 2시에서 51.24°(39.5-58.8°), 1시에서 52.45° (43.3-65.5°), 12시에서 44.09°(36.8-49.8°), 11시에서 40.71°(33.5-45.8°)였고 10시에서는 39.21°(34.1-44.6°)이었다. 전상방부(1시 및 2시)의 평균 알파각이 제일 컸고(P<0.01) 고관절Cam형 충돌과 관련된 영상의학적 소견의 빈도는 1시 및 2시에서 각각 15% 및 15.8%였다.
결론: 무증상 성인에서 cam형 충돌은 25.8%에서 발견 되었고 가장 호발하는 위치는 전상방부(1시 및 2시)였다. 한국에서 처음 시계 시스템으로 알파값을 측정한 결과를 보여주어 FAI에대한 관절경적 치료나 돌출부 제거술(bumpectomy)을 하는 수술의사들에게 도움이 될 것으로 생각된다.Ⅰ. INTRODUCTION 1
Ⅱ. MATERIALS AND METHODS 3
A. MATERIALS 3
B. METHODS 3
C. STATISTICAL ANALYSIS 4
Ⅲ. RESULTS 8
Ⅳ. DISCUSSION 11
Ⅴ. CONCLUSION 16
REFERENCES 17
국문요약 21Docto
The origin and diversification of MADS-box transcriptive factors in green plants
No full textThis Dataverse contains the predicted 3D structure of Type II proteins in Viridiplantae and MEF2s in other eukaryotes presented in "The origin and diversification of MADS-box transcriptive factors in green plants" by Jia-Peng Han, Jun-Nan Wan, Zhi-Lin Guan, Qing-Feng Wang, Tao Wan
A Ratiometric Two-Photon Fluorescent Probe for Tracking the Lysosomal ATP Level: Direct in cellulo Observation of Lysosomal Membrane Fusion Processes
No full text2
Cabardites unicornuta Zhao & Wu & Han 2023, sp. nov.
No full textCabardites unicornuta sp. nov. (Figs 6–8, 13, 14, 15, 20, 21) urn:lsid:zoobank.org:act: 2E593110-5ECA-4D9A-B14C-25D5D0DACBF4 Type-material. Holotype: 1♂, China, Hunan Province, Chenzhou, Mangshan Nature Reseve, 30.VII–7.VIII.2021, leg. J. Wu & Q. Lin, genit. prep. no. ztt-484-1. Paratypes: 1♂, Jiangxi Province, Yifeng, Mt. Guanshan, 21– 27.VII.2017, leg. H.L. Han, genit. prep. no. ztt-693-1; 4♂♂ Guangdong Province, Shaoguan City, Chebaling Natural Reserve, 28–30.V.2021, leg. M. R. Li & G. Fu, genit. prep. no. ztt-511-1, ztt-678-1, ztt-679-1, ztt-680-1.Published as part of Zhao, Ting-Ting, Wu, Jun & Han, Hui-Lin, 2023, Two new species and a new record of the genus Cabardites Volynkin & Černý (Lepidoptera, Erebidae, Arctiinae) from China, pp. 369-376 in Zootaxa 5339 (4) on page 372, DOI: 10.11646/zootaxa.5339.4.3, http://zenodo.org/record/830914
Garudinia ovata Zhao & Wu & Han 2023, sp. nov.
No full text<i>Garudinia ovata</i> sp. nov. <p>(Figures 1, 2, 13, 18, 22)</p> <p> <b>Type-material.</b> <b>Holotype:</b> 1♂, China, Yunnan Province, Xishuangbanna Prefecture, Yexianggu scenic spot, 4– 5.VIII.2018, leg. H.L. Han, J. Wu & M.R. Li, genit. prep. no. ztt-225-1, in NEFU. <b>Paratypes</b>: 1♀, China, Yunnan Province, Pu’er City, Jiangcheng County, 15–17.IX.2008, leg. H.L. Han & E. Liu, genit. prep. no. ztt-178-1; 1♂, China, Yunnan Province, Pu’er City, Jiangcheng County, 15.II.2014, leg. H.L. Han & G.H. Zu, genit. prep. no. ztt-181-1; 1♂, China, Yunnan Province, Pu’er City, Jiangcheng County, 1–2.VIII.2018, leg. H.L. Han, J. Wu & M.R. Li, genit. prep. no. ztt-232-1; 1♂, same data as holotype, genit. prep. no. ztt-233-1; in NEFU.</p>Published as part of <i>Zhao, Ting-Ting, Wu, Jun & Han, Hui-Lin, 2023, One new species and two new records of the genus Garudinia Moore, 1882 (Lepidoptera, Erebidae, Arctiinae, Lithosiini) from China, pp. 188-194 in Zootaxa 5256 (2)</i> on page 189, DOI: 10.11646/zootaxa.5256.2.6, <a href="http://zenodo.org/record/7745553">http://zenodo.org/record/7745553</a>
C-Jun N-terminal kinase 2 promotes liver injury via the mitochondrial permeability transition after hemorrhage and resuscitation
No full textHemorrhagic shock leads to hepatic hypoperfusion and activation of mitogen-activated stress kinases (MAPK) like c-Jun N-terminal kinase (JNK) 1 and 2. Our aim was to determine whether mitochondrial dysfunction leading to hepatic necrosis and apoptosis after hemorrhage/resuscitation (H/R) was dependent on JNK2. Under pentobarbital anesthesia, wildtype (WT) and JNK2 deficient (KO) mice were hemorrhaged to 30 mm Hg for 3 h and then resuscitated with shed blood plus half the volume of lactated Ringer's solution. Serum alanine aminotransferase (ALT), necrosis, apoptosis and oxidative stress were assessed 6 h after resuscitation. Mitochondrial polarization was assessed by intravital microscopy. After H/R, ALT in WT-mice increased from 130 U/L to 4800 U/L. In KO-mice, ALT after H/R was blunted to 1800 U/l (P < 0.05). Necrosis, caspase-3 activity and ROS were all substantially decreased in KO compared to WT mice after H/R. After sham operation, intravital microscopy revealed punctate mitochondrial staining by rhodamine 123 (Rh123), indicating normal mitochondrial polarization. At 4 h after H/R, Rh123 staining became dim and diffuse in 58% of hepatocytes, indicating depolarization and onset of the mitochondrial permeability transition (MPT). By contrast, KO mice displayed less depolarization after H/R (23%, P < 0.05). In conclusion, JNK2 contributes to MPT-mediated liver injury after H/R
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