1,721,206 research outputs found
The secreted BMP antagonist ERFE is required for the development of a functional circulatory system in Xenopus
No full textThree-dimensional visualization of an unusual pulmonary lymphoproliferation after COVID-19
No full textThe following case report details the case of a 40-year-old Caucasian patient who presented with dyspnea following a serologically confirmed mild-to-severe pulmonary infection with SARS-CoV-2. Chest computer tomography revealed a solitary ground-glass pulmonary nodule in the lower right lobe, measuring 2.1 cm in diameter. Video-assisted thoracoscopic surgery wedge resection revealed well-circumscribed lymphoid aggregates adjacent to the round, smaller airways, bronchioles, and blood vessels. IgKappa B exhibited a monoclonal polyclonal pattern, in contrast to the behavior exhibited by IgKappa A and IgLambda. In the following discussion, the lymphoid lesion was considered in the context of lymphoid hyperplasia, accompanied by an early infiltration of low-grade extranodal B cell lymphoma of the bronchus-associated lymphoid tissue (BALToma)
Untersuchungen zu Langzeitergebnissen nach totaler Laryngektomie beim fortgeschrittenen Larynxkarzinom im Vergleich zu alternativen Therapieansätzen
No full textBedeutung von CD44-Varianten für die Epithelial-Mesenchymale Transition in Krebszellen
No full textBreast cancer is one of the leading tumor types and is associated with high mortality. Research aims to develop new targets for early detection, prognosis assessment and also targets for potential new targeted therapies. The CD44 is used for the identification of cancer stem cells (CD44+/CD24-) and by alternative splicing isoforms with contained variable exons (CD44v2-10) exist besides the standard form of the CD44 (CD44s). By qRT-PCR the expression levels of the CD44 isoforms on the mRNA level and by Western blot on the protein level are determined for different cancer stem cell lines and the influence of the EMT inducers TNF-α and TGF-β is observed. Positive CD44 expression is found in all cell lines used, on the mRNA and protein levels. The total CD44 expression levels cannot be clearly assigned to any phenotype, possibly there is an association with tumor prognosis. The epithelial cell lines MDA-MB-468 and MCF-7 have low proportional CD44s expressions but increased variable isoforms. In contrast, the mesenchymal-like cell lines MDA-MB-231 and Panc-1 show high proportional CD44s expressions. A correlation of CD44s to the phenotype of the cell lines can be shown. BCSC1-5 are characterized by co-expression of epithelial and mesenchymal markers. These show low CD44s expressions and resemble epithelial MDA-MB-468 in the pattern of CD44 expression. Due to the influence of the EMT inducer TNF-α, an increase in total CD44 expression occurs without many significant changes in the proportions of individual CD44 isoforms. Due to the influence of the EMT inducer TGF-β, the total CD44 expression is reduced. Only isolated high CD44v6 expression occurs, like the CD44 expression pattern of the epithelial cell line MCF-7. By using EMT inducers, increasing expression of mesenchymal markers cannot be associated with increasing CD44s expression. The results raise the question of whether CD44v6 or total CD44 expression levels in addition to CD44s might be significant in the processes of EMT
Untersuchung von miRNA-Expressionsprofilen in Urinproben von Patientinnen mit Verdacht auf Ovarialkarzinom
No full textVergleichende Analyse des fibrotischen Lungenumbaues nach Lungen- und Stammzelltransplantation
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Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Inflammation and vascular remodeling in COVID-19 hearts
No full textA wide range of cardiac symptoms have been observed in COVID-19 patients, often significantly influencing the clinical outcome. While the pathophysiology of pulmonary COVID-19 manifestation has been substantially unraveled, the underlying pathomechanisms of cardiac involvement in COVID-19 are largely unknown. In this multicentre study, we performed a comprehensive analysis of heart samples from 24 autopsies with confirmed SARS-CoV-2 infection and compared them to samples of age-matched Influenza H1N1 A (n= 16), lymphocytic non-influenza myocarditis cases (n= 8), and non-inflamed heart tissue (n= 9). We employed conventional histopathology, multiplexed immunohistochemistry (MPX), microvascular corrosion casting, scanning electron microscopy, X-ray phase-contrast tomography using synchrotron radiation, and direct multiplexed measurements of gene expression, to assess morphological and molecular changes holistically. Based on histopathology, none of the COVID-19 samples fulfilled the established diagnostic criteria of viral myocarditis. However, quantification via MPX showed a significant increase in perivascular CD11b/TIE2 + —macrophages in COVID-19 over time, which was not observed in influenza or non-SARS-CoV-2 viral myocarditis patients. Ultrastructurally, a significant increase in intussusceptive angiogenesis as well as multifocal thrombi, inapparent in conventional morphological analysis, could be demonstrated. In line with this, on a molecular level, COVID-19 hearts displayed a distinct expression pattern of genes primarily coding for factors involved in angiogenesis and epithelial-mesenchymal transition (EMT), changes not seen in any of the other patient groups. We conclude that cardiac involvement in COVID-19 is an angiocentric macrophage-driven inflammatory process, distinct from classical anti-viral inflammatory responses, and substantially underappreciated by conventional histopathologic analysis. For the first time, we have observed intussusceptive angiogenesis in cardiac tissue, which we previously identified as the linchpin of vascular remodeling in COVID-19 pneumonia, as a pathognomic sign in affected hearts. Moreover, we identified CD11b + /TIE2 + macrophages as the drivers of intussusceptive angiogenesis and set forward a putative model for the molecular regulation of vascular alterations.National Institutes of Health http://dx.doi.org/10.13039/100000002European Research CouncilDeutsche Forschungsgemeinschaft 501100001659BRCCH 501100022242DeRegCOVIDDEFEATPANDEMicsBundesministerium für Bildung und Forschung http://dx.doi.org/10.13039/501100002347Medizinische Hochschule Hannover (MHH
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