4,174 research outputs found
Geneva's Use of Lies, Deceit, and Subterfuge, 1536-1563 Telling the Old, Old Story in Reformation France
No full textTelling the Old, Old Story in Reformation France Jon Balserak. it caused a bit of a stir among Calvin's circle of friends. Although his language is open to different interpretations, Calvin's identifying the new king's query as a threat ...</p
Rubin, Jon: Moscone being forgotten
No full textJon Rubin: I think that George has been forgotten. There are some stories that are easier to tell. Sometimes you tell the easy story, and the more nuanced story is lost. And George was an important and notable figure, and notable and wonderful guy, and he needs to be remembered for who he was. The tragedy of the end of his life should not define his life; that’s just the end of his life. It’s a tragedy, and there’s no question about it, but I think the lessons of his life are more important than the lessons of his death. And so whatever part I can take in telling that story and making sure that it’s out there to whatever extent it is out there… It’s a privilege to do it, it’s an honor, it’s a labor of love, and I’m delighted to be doin’ it
Digital story telling using iPods
No full textThis chapter describes the experience of using iPods with preservice early childhood educators in an introductory ICT course. The approach taken was to use the mobile devices, not as the object of study in themselves, but as cognitive tools to be used to complete a complex and authentic task. Students used the iPods in groups to create digital stories appropriate for very young children, in the style of a children’s picture book. The research explored the students’ responses to the task and the pedagogical affordances of the devices in the early childhood setting
Practical Tools for Telling Your Fulbright Story
No full textThis presentation was given to Fulbright participants during their pre-departure orientationThis session provide a practical overview of the tools used for online storytelling and how Fulbright participatns can maximize the impact of their story online. Bob Tryanski, professional storyteller, introduces the art of storytelling by discussing “what’s in a name,” using the example of his own name, and getting the audience to share something about their own names. These conversations demonstrate how sources for story material are all around us. Storytelling is a relational versus transactional activity; the angel is in the details; and the art is in the listening. Jon Giullian, Librarian for Slavic and Eurasian Studies at the University of Kansas, asks audience to consider the origin of their Fulbright story and how they can tell their story forward to inspire future Fulbright participants. He describes various categories of platforms for telling a story, including social media, blogs, vlogs, website, storytelling apps, and public forums. He also share safety tips and security guidelines recommended for digital content creation
The Greenland Ice Sheet as a hotspot of phosphorus weathering and export in the Arctic
No full textThe contribution of ice sheets to the global biogeochemical cycle of phosphorus is largely unknown, due to the lack of field data. Here we present the first comprehensive study of phosphorus export from two Greenland Ice Sheet glaciers. Our results indicate that the ice sheet is a hot spot of phosphorus export in the Arctic. Soluble reactive phosphorus (SRP) concentrations, up to 0.35?µM, are similar to those observed in Arctic rivers. Yields of SRP are among the highest in the literature, with denudation rates of 17–27?kg?P?km?2?yr?1. Particulate phases, as with nonglaciated catchments, dominate phosphorus export (>97% of total phosphorus flux). The labile particulate fraction differs between the two glaciers studied, with significantly higher yields found at the larger glacier (57.3 versus 8.3?kg?P?km?2?yr?1). Total phosphorus yields are an order of magnitude higher than riverine values reported in the literature. We estimate that the ice sheet contributes ~15% of total bioavailable phosphorus input to the Arctic oceans (~11?Gg?yr?1) and dominates total phosphorus input (408?Gg?yr?1), which is more than 3 times that estimated from Arctic rivers (126?Gg?yr?1). We predict that these fluxes will rise with increasing ice sheet freshwater discharge in the future
Preparation and Evaluation of Polymer Coated Magnetic Nanoparticles for Applications in Gene Delivery
No full textWith the advent of powerful gene editing tools such as CRISPR/Cas9, advances in gene therapy have gained a second wind. Despite this, disease therapy still has not progressed beyond clinical trials due to limitations in current delivery methods. The work presented in this thesis studies the development of a non-viral gene delivery method which is the nanomagnetic transfection method, which is the delivery of genes to cells using magnetic nanoparticles (MNPs) with a cationic surface charge and an external magnetic field. The advantage of nanomagnetic transfection over other non-viral chemical methods is the low dosage required to transfect cells coupled with a short transfection time. The presence of an external magnet provides targeting functionality, whereby the MNPs carrying the gene of interest are pulled towards the cells, thus increasing the efficiency of cell to MNP contact.The research looks at the synthesis of MNPs using thermal decomposition to obtain particles with a narrow size distribution and exhibiting a combination of Brownian and Neel relaxation. The MNPs were coated with polyethyleneimine (PEI), which binds and condenses DNA to deliver into cells for protein expression. PEI is known to be toxic to cells at high concentrations, hence PEI not bound to MNPs were removed using dialysis. A unique study observing the gradual loading of PEI coating on MNPs using AC susceptometry (ACS) is described. ACS provided information on the MNP coating and aggregation process that was not accessible through dynamic light scattering (DLS) due to the additional presence of non-magnetic polymer particulates in the suspensions. In combination with complementary structural characterization techniques, a simple method was derived to obtain dense, uniform PEI coatings affording high-stability suspensions without excessive quantities of unbound PEI to reduce cytotoxic effects. This method can be used for improving coating and functionalization therefore advancing MNP-drug/gene delivery studies.The PEI-coated MNPs were subsequently studied for their transfection capabilities in HeLa cells and compared to commercial MNP transfection agents. It was found that nanomagnetic transfection had higher GFP reporter expression compared to Lipofectamine and PEI. The parameters affecting transfection activity were determined in order to improve transfection rates of synthesized MNPs. A trade-o_ between transfection efficiency and cytotoxicity was observed, where the presence of unbound PEI improved transfection but affected cell viability. To overcome this, polymers and block-copolymers with a lower charge density should be developed.The proton-sponge effect, which is the mechanism of MNP-PEI escape from the endolysosome was studied by measuring the AC susceptibility of MNP-PEI in live cells. However, the low transfection efficiency of MNP-PEI and low sensitivity of the AC susceptometer made it difficult to obtain conclusive evidence. A novel study using Raman spectroscopy to obtain fingerprint spectra of the MNP-PEI complexes and to determine their localization in cells is reported. Individual spectra of MNP and PEI were obtained, as well as the area map of the cell, however the localization of MNPs within the cell was not possible due to the limited sensitivity of the Raman spectrometer. Finally, the effect of the MNP-PEI transfection agents on cells were identified. It was observed that MG-63 and HeLa cells expressed increased cell stress with the formation of actin stress fibres and increased cell adhesion. Between the two transfection components, PEI antagonized the cell adhesion effect compared to MNPs. In addition, the genes associated with actin fibres and cell adhesion were identified, which were ACTA2, ACTN1, MVCL, VCL, P4HA2, PCDHB12, SVIL, and TGFBI, which showed increased expression to MNP-PEI treatment. Collectively, the study conducted reports the development of an MNP transfection agent, from synthesis to application
Story-telling for upper grade teachers,
No full textBibliography: p. 265-269; "Stories for telling": p. 270-296.Mode of access: Internet
Generation of long-chain fatty acids by hydrogen-driven bicarbonate reduction in a low-temperature alkaline hydrothermal vent simulator
No full textRaw analytical Data from the publication Generation of long-chain fatty acids by hydrogen-driven bicarbonate reduction in a low-temperature alkaline hydrothermal vent simulator by Graham Purvis, Lidija Siller, Archie Crosskey, Jupiter Vincent, Corrine Wills, Jake Sheriff, Cijo Xavier and Jon Telling. Data from the following instruments: X-ray photoelectron spectroscopy, FTIR, pyrolysis-gas chromatography/mass spectrometry, 1HNMR, Thermogravimetric analysis, total organic combustion and ion chromatography
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