898 research outputs found
Number of texts per author vs. accuracy of MDA classifier.
No full text<p>This graph investigates accuracy versus the size of the training dataset for the MDA case, with a fixed set of 100 function words, for the benchmark English corpus of known texts given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0054998#pone-0054998-t001" target="_blank">Table 1</a>. The upper curve shows the LOO-CV accuracy of MDA, as a function of the number of author texts, by deliberately limiting the size of the training dataset. The lower curve shows the MDA accuracy that is obtained by inputting the hold-out texts to the classifier at each step.</p
Towards an MDA Oriented Methodology
No full textThe author describes the need to introduce a methodology named MDA or Model Driven Architecture based on the concept of Open Distributed Application Construction (ODAC) to be used as a guide in the development of complex distributed applications. The reasons offered for the need of MDA are: 1. The emerging and proliferation of middleware technology. 2. The interoperability of the different middleware. 3. High cost of migration. 4. Problems of portability from one platform to another. And the advantages of MDA are: 1. An architecture based on the MDA approach will always be ready to deal with tomorrow’s “next big thing” 2. MDA will allow easier integration of applications and facilities across middle ware boundaries. 3. Allows cross-platform interoperability. Finally, the principles of the MDA will also be conveyed
Altered immunolocalization of FGF23 in murine femora metastasized with human breast carcinoma MDA-MB-231 cells
No full textIntroduction After the onset of bone metastasis, tumor cells appear to modify surrounding microenvironments for their benefit, and particularly, the levels of circulating fibroblast growth factor (FGF) 23 in patients with tumors have been highlighted. Materials and methods We have attempted to verify if human breast carcinoma MDA-MB-231 cells metastasized in the long bone of nu/nu mice would synthesize FGF23. Serum concentrations of calcium, phosphate (Pi) and FGF23 were measured in control nu/nu mice, bone-metastasized mice, and mice with mammary gland injected with MDA-MB-231 cells mimicking primary mammary tumors. Results and conclusions MDA-MB-231 cells revealed intense FGF23 reactivity in metastasized lesions, whereas MDA-MB-231 cells cultured in vitro or when injected into the mammary glands (without bone metastasis) showed weak FGF23 immunoreactivity. Although the bone-metastasized MDA-MB-231 cells abundantly synthesized FGF23, osteocytes adjacent to the FGF23-immunopositive tumors, unlike intact osteocytes, showed no FGF23. Despite significantly elevated serum FGF23 levels in bone-metastasized mice, there was no significant decrease in the serum Pi concentration when compared with the intact mice and mice with a mass of MDA-MB-231 cells in mammary glands. The metastasized femora showed increased expression and FGFR1 immunoreactivity in fibroblastic stromal cells, whereas femora of control mice showed no obvious FGFR1 immunoreactivity. Taken together, it seems likely that MDA-MB-231 cells synthesize FGF23 when metastasized to a bone, and thus affect FGFR1-positive stromal cells in the metastasized tumor nest in a paracrine manner
Overexpression of hsa-miR-512-5p decreases proliferation in breast cancer line MDA-MB-453
No full textUna variedad de miRNAs cumplen un rol importante en la regulación del cáncer de mama, considerado como el tumor maligno más común en mujeres. hsa-miR-512-5p se ha documentado como oncomiR, y también como supresor tumoral en diversos tipos de cáncer, sin embargo, no está claro su rol en tumores mamarios. Debido a que, las células tumorales de mama no expresan naturalmente hsa-miR-512-5, esta investigación tuvo como objetivo evaluar el efecto de este miRNA en la proliferación de la línea de cáncer MDA-MB-453 y esclarecer su rol en la línea tumoral. A partir de esto, se transfectaron cultivos in-vitro MDA-MB-453 con el mimic para hsa-miR-512-5p, posteriormente fue evaluada la proliferación mediante el kit CyQuant® y los datos fueron leídos por fluorescencia. La validación de la transfección se hizo a través de qPCR usando el método 2^(-ΔΔCt). Los resultados indican que hsa-miR-512-5p disminuye significativamente la proliferación celular en la línea de cáncer de mama (p≤0,05). Investigaciones en otros modelos tumorales soportan estos resultados, demostrando que miR-512-5p regula vías de señalización que inducen apoptosis, favoreciendo la expresión de caspasas e inhibiendo hTERT-3’UTR telomerasa. Aunque son necesarios más estudios que evalúen el rol que tiene el microRNA en la tumorogénesis, esta investigación propone un posible efecto del posible efecto que hsa-miR-512-5p podría estar jugando en la línea MDA-MB-453. Esto abre nuevas expectativas para ampliar la investigación de este miRNA en cáncer de seno.A variety of miRNAs play an important role in the regulation of breast cancer, considered the most common malignant tumor in women. hsa-miR-512-5p has been documented as an oncomiR, and as a tumor suppressor in various types of cancer, however, its role in mammary tumors is not clear. Since breast tumor cells do not naturally express hsa-miR-512-5, this research aimed to evaluate the effect of this miRNA on the proliferation of the MDA-MB-453 cancer line and to clarify its role in the tumor line. From this, in-vitro MDA-MB-453 cultures were transfected with the mimic for hsa-miR-512-5p, proliferation was subsequently evaluated using the CyQuant® kit and the data was read by fluorescence. The validation of the transfection was done through qPCR using the 2^(-ΔΔCt) method. The results indicates that hsa-miR-512-5p significantly decreases cell proliferation in breast cancer line (p≤0.05). Investigations in other tumor models support these results, demonstrating that miR-512-5p regulates signaling pathways that induce apoptosis, favoring caspase expression and inhibiting hTERT-3'UTR telomerase. Although more studies are needed to evaluate the role of microRNA in tumorigenesis, this research suggests a possible effect that hsa-miR-512-5p could be playing in the MDA-MB-453 line. This opens new expectations to expand the investigation of this miRNA in breast cancer.Biólogo (a)Pregradohttps://orcid.org/0000-0001-5146-650
Harvesting software systems for MDA-based reengineering
No full textIn this paper we report on a feasibility study in reengineering legacy systems towards a model-driven architecture (MDA). Steps in our approach consist of (1) parsing the source code of the legacy system according to a grammar; (2) mapping the abstract syntax trees thus obtained to a grammar model that is defined in the Meta-Object Facility (MOF); (3) using model to model (M2M) transformations to turn the grammar model into a generic meta-model, called Generic-AST, in which information about software systems can be stored in a language-independent way; (4) mapping the GenericAST models, again using M2M transformations, to UML models that can be either used for code generation or for documentation purposes. The steps have been implemented in a prototype model harvesting tool that is based on Arc-Styler, the MDA environment provided by Interactive Objects. Our paper presents this approach, and reports on our experiences in applying the method to a 178 KLOC production system from the insurance domain written in PL/SQL.Software TechnologyElectrical Engineering, Mathematics and Computer Scienc
Exploring the novels of Zakes Mda
No full textLecture series presented by Associate Professor Gail Fincham, Department of English, University of Cape Town. This lecture series, focusing on the novels of famous South African author Zakes Mda, will be of interest to students of African literature and lovers of literature more generally
Malondialdehyde (MDA) and 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels in canine serum: establishing reference intervals and influencing factors
No full textAuthor contributions:
Perez-Montero B.: Investigation, conceptualization, performing analytical methodology, data curation and writing original draft. Fermin-Rodriguez ML.: Conceptualization, supervision and reviewing original draft. Portero-Fuentes M.: Sample collection, reviewing original draft. Sarquis J.: IFAT methodology application, reviewing original draft. Caceres S.: Serum cortisol quantification, reviewing original drats. Illera del Portal J.C.: Serum cortisol quantification, providing resources. De Juan L.: Project administration, providing resources and reviewing original draft. Miro G.: Project administration, supervision and reviewing original draft. Cruz-Lopez F.: Supervision, data curation, validation of results, writing, reviewing and editing original draftBackground: Mounting evidence suggests that malondialdehyde (MDA) and 8-hydroxy-2'-deoxyguanosine (8-OHdG) are valuable biomarkers of lipid and nucleic acid oxidation in numerous canine diseases. However, their application in clinical settings is limited due to the absence of reference intervals (RI) and the analytical inconsistencies. Therefore, this study aimed to characterize serum MDA and 8-OHdG concentrations in dogs, to establish assay-specific RI, and to identify biological, haematological and biochemical factors influencing these markers.
Methods: A total of 190 clinically healthy dogs were recruited, including pet dogs, working dogs and shelter dogs. Serum MDA concentration was measured by the Thiobarbituric Acid Reactive Substances (TBARS) assay, while 8-OHdG levels were determined by using a competitive ELISA. RI were established by non-parametric methods. Potential associations between oxidative stress (OS) biomarkers and multiple biological, haematological and biochemical factors were assessed using multivariate regression models.
Results: RI for serum MDA (1.85-14.51 µM) and 8-OHdG (0.06-0.75 ng/mL) were established in the reference population (144 and 143 dogs, respectively). The multivariate regression model for MDA revealed a positive association with total cholesterol concentration, and a negative association with monocyte count. 8-OHdG level was positively associated with urea concentration. Notably, both models also revealed a significant association between MDA and 8-OHdG. Biological factors, including the age and size of the animals, did not exert a significant influence on the results.
Conclusions: This is the first study to establish serum RI for MDA and 8-OHdG in a large and diverse canine population. Additionally, the multivariate regression models identified relevant haematological and biochemical, but not biological factors that should be considered when interpreting the results. These findings could significantly enhance the application of MDA and 8-OHdG as biomarkers in clinical settings, and promote further exploration of their value in canine diseasesDepto. de Medicina y Cirugía AnimalDepto. de FisiologíaDepto. de Sanidad AnimalFac. de VeterinariaCentro de Vigilancia Sanitaria Veterinaria (VISAVET)TRUEpu
Model Transformation with ATL into MDA from CIM to PIM Structured through MVC
No full textAbstractModels transformation is the most important key in Model Driven Architecture (MDA). The first transformation in MDA is Computing Independent Model (CIM) to Platform Independent Model (PIM) transformation, the second is PIM to Platform Specific Model (PSM) transformation. This latter transformation deals the two less abstract levels, PIM and PSM, for that most researchers focused on this kind of transformation. Nevertheless, the top level of abstraction, CIM, and its transformation to PIM is rarely discussed in research topics. Our goal in this paper is to represent an approach that allows mastering transformation from CIM to PIM in accordance with MDA. Our method based on creating a good CIM level, using construction rules, to facilitate the transformation to PIM level. Next, our transformation rules, implemented with Atlas Transformation Language (ATL), ensure a semi-automatic transformation from CIM to PIM. Our approach conforms to MDA, because it allows considering the business dimension in the CIM level, and it allows modelling this latter level by using Business Process Model and Notation (BPMN), this latter is the OMG standard for modelling the business process. However, we founded on UML to model PIM level, because UML is advocated by MDA in PIM. Our proposal results a set of classes, organized according to the Model View Controller (MVC)
Semantic model-driven development of web service architectures.
No full textBuilding service-based architectures has become a major area of interest since the advent of Web services. Modelling these architectures is a central activity. Model-driven development is a recent approach to developing software systems based on the idea of making models the central artefacts for design representation, analysis, and code generation.
We propose an ontology-based engineering methodology for semantic model-driven composition and transformation of Web service architectures. Ontology technology as a logic-based knowledge representation and reasoning framework can provide answers to the needs of sharable and reusable semantic models and descriptions needed for service engineering. Based on modelling, composition and code generation techniques for service architectures, our approach provides a methodological framework for ontology-based semantic service architecture
Survey and mass drug administration (MDA) timelines.
No full textSurvey and mass drug administration (MDA) timelines.</p
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