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Characterization of unique population of human pre-naïve B cells with regulatory functions
Get PDF학위논문 (석사)-- 서울대학교 대학원 : 의학과 해부학 전공, 2013. 2. 김항래.서론: 사람의 말초혈액 내에 순환 하고 있는 pre-naïve B 세포는 transitional B 세포와 naïve B 세포의 중간단계의 특징을 보이는 것으로 밝혀져 있다. 그러나 pre-naïve B 세포의 기능에 대해서 자세히 알려진 바가 없다. 다만, 생쥐에서 사람 pre-naïve B 세포와 발달단계가 유사한 T2-marginal zone precursor(MZP) B 세포가 interleukin(IL) -10을 생성하는 조절세포로서(regulatory B cells, Bregs) 면역억제기능을 하는 것으로 알려져 있다. 따라서 본 연구에서는 사람 pre-naïve B 세포가 IL-10을 생성함으로 T 세포 증식과 cytokine 생성을 억제하는 조절세포로의 기능이 있는지 알아보고자 하였다.
방법: 사람의 말초혈액에서 pre-naïve B 세포를 분리하고, CD154(CD40L) 발현 세포를 이용한 CD40의 자극 조건에서 IL-10 생성능을 flow cytometry와 효소면역분석법으로 분석하였다. 또한 pre-naïve B 세포를 CD40의 자극 조건에서 CD4+ T 세포를 같이 배양하여 T 세포의 증식과 cytokine 생성에 미치는 영향을 flow cytometry와 효소면역분석법으로 분석하였다.
결과: 건강한 사람으로부터 분리한 pre-naïve B 세포가 CD40 자극 하에 naïve B 세포, memory B 세포에 비해서 IL-10을 의미 있게 많이 생성함을 확인하였다. 또한 pre-naïve B 세포가 CD4+ T 세포의 증식과 cytokine 생성을 억제하였다. 반면, 자가 면역질환인 전신성홍반성루푸스(루푸스, SLE) 환자의 pre-naïve B 세포는 CD40 자극을 주어도 정상인의 pre-naïve B 세포만큼의 IL-10를 생성하지 못 하였다.
결론: 따라서 pre-naïve B 세포는 사람 말초혈액에 존재하는 독특한 B 세포군로서 정상인의 면역반응을 조절하는 세포로의 역할을 보이며, 면역학적 항상성과 자가 항원에 대한 관용 유지에 매우 중요한 역할을 할 것으로 기대된다.초록 1
목차 3
표 및 그림 목록 5
서론 6
실험재료 및 방법 13
1. 정상인과 루푸스 환자 모집 13
2. 시약 및 세포 준비 13
3. 세포 배양 14
4. Cytokine 생성능력 측정 15
5. 세포증식 측정 16
6. 통계분석 17
결과 18
1. Pre-naïve B세포는 CD40 자극을 주었을 때 IL-10을 생성함. 18
2. Pre-naïve B 세포가 CD4+ T 세포의 증식 및 cytokine 을 억제함. 23
3. 루푸스 환자의 pre-naïve B 세포는 IL-10을 적게 생성함. 26
고찰 29
참고문헌 33
초록 (영문) 37Maste
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
근로형태 다양화에 따른 인적자원개발 지원 방안
No full textThis study reveals that flexible work is becoming more a trend rather than just a slogan around the world. More and more companies are trying to adopt more flexible work schedules partly as means of compensation for workers but also to increase worker productivity. It is a matter of time flexible work will be adopted more widely by Korean companies. This study sheds light on how to promote this system by suggesting ways to develop the key competencies demanded by increased work flexibility.
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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