309 research outputs found
Satellite bands of absorption in alkali vapor–rare gas mixtures
No full textSubmission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2025-12-01The student, Stephen Messing, accepted the attached license on 2023-12-06 at 20:38.The student, Stephen Messing, submitted this Thesis for approval on 2023-12-06 at 20:52.This Thesis was approved for publication on 2023-12-07 at 10:50.DSpace SAF Submission Ingestion Package generated from Vireo submission #20164 on 2024-03-01 at 13:32:43Absorption spectra for alkali-rare gas spectral satellites and alkali dimers in potassium, rubidium, and cesium are presented. Previous investigations of alkali-rare gas spectral satellites are incomplete, causing many of these satellites to remain unidentified or ill-characterized in the literature. The data presented in this work were captured by illuminating heated alkali vapor-rare gas cells with a xenon lamp and recording the spectra using a Yokogawa 6370D Optical Spectrum Analyzer. To generate a sufficient alkali vapor number density to observe satellites, the cells were placed in an oven and heated to temperatures between 353 K and 513K. The knowledge of alkali-rare gas blue satellites has the potential to be significant for the creation of low-divergence, high-power lasers for application as directed energy weapons and in anti-missile defense measures
Frances Burney and her readers : the negotiated image
No full textIn Frances Burney and Her Readers, Anna Paluchowska-Messing traces the rugged trajectory marked by the literary career of Frances Burney, the English eighteenth-century novelist, diarist and playwright. The study highlights the techniques Burney employed in her texts for projecting a favourable self-image, and sets them against the changing conventions in culture consumption and appreciation. More broadly, the study addresses the concept of women’s literary celebrity, which in late eighteenth-century England remained at odds with contemporary ideals of feminine respectability and prescribed domesticicity. In Paluchowska-Messing’s representation, Burney’s story showcases the dilemmas an eighteenth-century author must face at different stages of her career from debutante to that of an acclaimed literary figure, and possible solutions she might choose in order to court celebrity without losing respectability
Treatise on Invertebrate Paleontology
No full textThis volume is edited by Paul Selden, authors are Hans Hess and Charles G. Messing, coordinating author is William I. Ausich. This is the first volume to be published in an extensive revision of the Class Crinoidea. The present volume deals with the Subclass Articulata that contains all post-Paleozoic and living crinoids. The descriptions are preceded by an introduction, a chapter on the morphology of articulate crinoids, a glossary of important terms, and an overview of classification. The reference list is comprehensive for this volume.https://nsuworks.nova.edu/occ_facbooks/1009/thumbnail.jp
Functional and evolutionary genomics studies of maize seed development
No full textThis dissertation is divided into two chapters with a common theme of investigating the role of several genes in seed development. Studies of the genetic basis of corn seed development provide not only answers to basic biological questions, but also have significant implications for nutritional and industrial uses. For example, the relative concentrations of different types of storage proteins in maize, called zeins, greatly affect the amount of essential amino acids lysine, methionine, and tryptophan, which are important for human and animal nutrition (Mertz, et al. 1964; Messing and Fisher 1991). The interaction of protein bodies with starch granules also affects kernel hardness and consequently the transportation and storage of corn grains (Wu, et al. 2010). The quantity and quality of another important household commodity – corn oil – also depends on the biosynthesis of triacylglycerols and their storage into lipid bodies which are mainly found in the embryo (White and Weber 2003). Clearly, understanding the many pathways involved in seed development is an important step towards improving its uses. In the first chapter, the conservation of regulatory factors controlling gene expression of zeins throughout the Poaceae were investigated by taking advantage of oat-maize addition lines or OMAs. Oats and maize belong to two different subfamilies of the Poaceae, but it was possible to cross pollinate the two and obtain seeds, where one maize chromosome at a time can be added to the whole set of oat chromosomes (Kynast, et al. 2001; Rines, et al. 2009). Therefore, one can examine whether oat has regulatory factors that can cause the expression of genes added by the single maize chromosomes, but regulated by a different maize chromosome. The results showed that recently diverged genes of the prolamin gene family, the α-, β-, and δ-zeins, were not expressed, whereas the older γ-zein genes were expressed. Further studies also showed that the oat homolog of a known regulator of zein gene expression called Prolamin box binding factor 1 (Pbf1) was able to trans-activate γ-zein expression in transient expression assays, indicating that it can substitute for the function of its maize homolog. The wheat Dx5 gene, presumably the founding member of the prolamin gene family (Xu and Messing 2009), is also expressed in a maize transgenic line, even when zein transcription factors, Pbf1 and O2, are knocked down. Overall, our data indicates that the regulation of gene expression of old copies of seed protein genes is conserved, whereas the regulation of younger copies seems to have diverged. Mutant collections are also great resources for identifying genes involved in seed development. Many researchers have used two mutant resources to study maize seed development – a collection of defective kernel (dek) mutants developed from EMS mutagenesis and mutants from Mutator insertion collections. Some of the genes that were identified using these two resources encode a heat shock binding protein (Fu, et al. 2002), a chloroplast DNA polymerase (Udy, et al. 2012), an RNA splicing factor (Fouquet, et al. 2011), and several enzymes (Lid, et al. 2002; Wang, et al. 2014). The second chapter uses a new insertion mutant resource that has been developed based on a Ds transposable element tagged with GFP (Dsg) (Li, et al. 2013). Selection of a seed with a defective kernel phenotype has led to the identification of dek38-Dsg, a recessive lethal mutant that encodes a co-chaperone protein called Tel2-interacting protein 2 (TTI2). TTI2 interacts with two other co-chaperones called Telomere maintenance 2 (Tel2) and Tel2-interacting protein 1 (TTI1) to form the TTT complex that is required to maintain steady-state levels of phosphatidylinositol 3-kinase-related kinases (PIKKs) which are essential for development (Hurov, et al. 2010; Takai, et al. 2010). As a co-chaperone for HSP90, the TTT complex can interact directly with PIKKs to aid in their proper protein folding and assembly into functional complexes. Reversion analysis and multiple Dsg excision footprint alleles established the linkage of the gene to the phenotype. Histological sections of developing seeds show that many aspects of development are affected in dek38-Dsg. The arrest of embryo development at the transition stage is similar to the PIKK Target of rapamycin (TOR) mutation in Arabidopsis and is consistent with reduced level of TOR protein in dek38-Dsg. Pollen transmission problems, as shown by the significantly lower number of GFP kernels when dek38-Dsg is used as a male, indicate that TTI2 is important for male reproductive cell development. Cloning of maize Tel2 and Tti1 homologs and yeast two-hybrid assays show that the interaction of TEL2 to TTI1 and TTI2 is conserved in maize. Overall, the results open up new lines of investigations into the roles of co-chaperones in seed development, and show the advantages of the Dsg insertion collection in maize for functional analysis of a gene. To sum up, my dissertation presented evidence of the conservation of gene expression in older copies of seed storage protein genes in maize, and showed the utility of a new mutant resource in maize by characterizing the effects of Tti2 mutation for the first time in plants. Our findings provide new avenues to the roles of gene expression and genome evolution, as well as the role of co-chaperones in seed development. For example, how do promoters or DNA binding proteins diverge after gene amplification? What are the direct targets of Tti2 during seed development? The approaches taken here have advanced our understanding of the genetic basis of seed development and allowed us to suggest further directions in our quest to further improve one of our most important crops through molecular breeding. References Fouquet R, et al. 2011. Maize Rough Endosperm3 Encodes an RNA Splicing Factor Required for Endosperm Cell Differentiation and Has a Nonautonomous Effect on Embryo Development. Plant Cell 23: 4280-4297. Fu S, Meeley R, Scanlon MJ 2002. empty pericarp2 Encodes a Negative Regulator of the Heat Shock Response and Is Required for Maize Embryogenesis. Plant Cell 14: 3119–3132. Hurov KE, Cotta-Ramusino C, Elledge SJ 2010. A genetic screen identifies the Triple T complex required for DNA damage signaling and ATM and ATR stability. Genes Dev 24: 1939-1950. Kynast RG, et al. 2001. A Complete Set of Maize Individual Chromosome Additions to the Oat Genome. Plant Physiology 125: 1216-1227. Li Y, Segal G, Wang Q, Dooner HK. 2013. Gene tagging with engineered Ds elements in maize. In: Peterson T, editor. Plant Transposable Elements: Methods and Protocols: Humana Press. p. 83-99. Lid SE, et al. 2002. The defective kernel 1 (dek1) gene required for aleurone cell development in the endosperm of maize grains encodes a membrane protein of the calpain gene superfamily. Proc Natl Acad Sci U S A 99: 5460-5465. Mertz ET, Bates LS, Nelson OE 1964. Mutant gene that changes protein composition and increases lysine content of maize endosperm. Science 145: 279–280. Messing J, Fisher H 1991. Maternal effect on high methionine levels in hybrid corn. Journal of Biotechnology 21: 229-237. Rines HW, et al. 2009. Addition of individual chromosomes of maize inbreds B73 and Mo17 to oat cultivars Starter and Sun II: maize chromosome retention, transmission, and plant phenotype. Theor Appl Genet 119: 1255-1264. Takai H, Xie Y, de Lange T, Pavletich NP 2010. Tel2 structure and function in the Hsp90-dependent maturation of mTOR and ATR complexes. Genes Dev 24: 2019-2030. doi: 10.1101/gad.1956410 Udy DB, Belcher S, Williams-Carrier S, Gualberto JM, Barkan A 2012. Effects of Reduced Chloroplast Gene Copy Number on Chloroplast Gene Expression in Maize. Plant Physiology 160: 1420-1431. Wang G, et al. 2014. Proline responding1 Plays a Critical Role in Regulating General Protein Synthesis and the Cell Cycle in Maize. Plant Cell 26: 2582-2600. White PJ, Weber EJ. 2003. Lipids of the kernel. In: White PJ, Johnson LA, editors. Corn: Chemistry and Technology. St. Paul, Minnesota: American Association of Cereal Chemists, Inc. Wu Y, Holding DR, Messing J 2010. Gamma-Zeins are essential for endosperm modification in quality protein maize. Proc Natl Acad Sci U S A 107: 12810–12815. Xu JH, Messing J 2009. Amplification of prolamin storage protein genes in different subfamilies of the Poaceae. Theor Appl Genet 119: 1397-1412.Ph.D.Includes bibliographical referencesby Nelson S. Garci
Antoni Messing- co-creator of the Sacred Virgin Mary's statue located in front of the Reformed Church and co-author of series of tombstones patterned after it and raised at the cemeteries of Warsaw
No full textAntoni Messing (ca. 1821-1867) the owner of the stone workshop located in Warsaw on 6 Powązkowska Street (mtge. 27C) is currently most famous for one monument- the Statue of the Virgin Mary of Immaculate Conception which was placed in front of the Church of St Antony of Padua on Senatorska Street (1851). What made this monument different from other independently standing monuments was the use of lanterns which at evening time illuminated the statue of the Virgin (1853). The innovative idea spread not only around Warsaw, but also outside the city boundaries.
References to the monument elevated by Messing were not limited to the way and form of illuminating the statue. The inventory research conducted on Warsaw cemeteries ena-ble the extraction of a group of tombstones imitating the shape and the decor of the plinth of the statue of the Virgin. The number of examples of this collection of tombstones numbers 19. Their execution dates back to the period 1853-1874 - with one exception only, all of them were elevated during the period of Antoni Messing’s ownership of the stone workshop. All of them represent the same commemoration in the form of a crucifix located on a plinth. Exam-ples can be separated into two groups. One, comprising 8 tombstones, the closest to the origi-nal, the other, comprising 11 examples preserves the architectural structure without the sculp-tural decor. The origin of the formal concept is to be traced in the project of Henryk Marconi’s garden vase designed for Wilanowski Park (ca. 1845-1851) as well as the finishing elements of the Stanisław and Antoni Potocki’s tombstones. Consequently, the contribution of Messing consists in the creation of the series of tombstones modeled on the statue of the Virgin Mary rather than the originality of the project
Waste Houses: Messing up The Netherlands
No full textThe Netherlands has the ambition to transition to a fully circular economy before 2050. Between this future and where we stand now, there is still a large gap. The Netherlands produces 60 million tonnes of waste per year. This fact contains two problems that this project aims to deal with: 1) the heigh of this number in the first place, and 2) that approximately 20% of the waste does not find its way back into the system. In 2020, 7.6 million tonnes of waste was incinerated and 32.7 million tonnes of waste was exported to non-EU countries, where waste often ends up in landfill or is send for incineration with adverse health effects.This project takes the radical stance to stop incineration and export, which means the Netherlands must take responsibility for the waste it produces. For much of the waste that currently follows one of these trajectories, there are no adequate solutions for reuse or recycling. Hence, we designed a system of waste collection, sorting, and storage where materials can be stored in waste houses until they find their way back into the system (problem 1). The piling up of the waste will create awareness of the consequences of unresponsible consumption, affecting the behaviour of people through confrontation (problem 2).The system we design aims to create a disruption of the existing linear system at different levels. The large-scale societal perception of production-consumption-waste generation will slowly change, while the waste houses will create a sense of urgency at the small scale. This will stimulate niche innovations to find innovative solutions to deal with waste that is stored. Our project is thus both an instigator of change and part of the change itself in the transition to the circular economy.If the project is successful, the waste houses will gradually become obsolete as consumption and waste production go down. In the far future, the former waste houses can house different functions, or they can be demolished in a circular way, returning the materials into the resource loop.AR2U086 R&D Studio – Spatial Strategies for the Global MetropolisArchitecture, Urbanism and Building Sciences | Urbanis
Antoni Messing- co-creator of the Blessed Virgin Mary\u27s statue located in front of the Reformed Church and co-author of series of tombstones patterned after it and raised at the cemeteries of Warsaw
No full textAntoni Messing (ca. 1821-1867) the owner of the stone workshop located in Warsaw on 6 Powązkowska Street (mtge. 27C) is currently most famous for one monument- the Statue of the Virgin Mary of Immaculate Conception which was placed in front of the Church of St Antony of Padua on Senatorska Street (1851). What made this monument different from other independently standing monuments was the use of lanterns which at evening time illuminated the statue of the Virgin (1853). The innovative idea spread not only around Warsaw, but also outside the city boundaries.
References to the monument elevated by Messing were not limited to the way and form of illuminating the statue. The inventory research conducted on Warsaw cemeteries enable the extraction of a group of tombstones imitating the shape and the decor of the plinth of the statue of the Virgin. The number of examples of this collection of tombstones numbers 19. Their execution dates back to the period 1853-1874 - with one exception only, all of them were elevated during the period of Antoni Messing’s ownership of the stone workshop. All of them represent the same commemoration in the form of a crucifix located on a plinth. Examples can be separated into two groups. One, comprising 8 tombstones, the closest to the original, the other, comprising 11 examples preserves the architectural structure without the sculptural decor. The origin of the formal concept is to be traced in the project of Henryk Marconi’s garden vase designed for Wilanowski Park (ca. 1845-1851) as well as the finishing elements of the Stanisław and Antoni Potocki’s tombstones. Consequently, the contribution of Messing consists in the creation of the series of tombstones modelled on the statue of the Virgin Mary rather than the originality of the project.
 
Policing Immigrants: Fear of Deportations and Perceptions of Law Enforcement and Criminal Justice
No full textabstract: This study examined the relationship between the fear of deportation and perceptions of law enforcement, the criminal justice system, and the willingness to report crimes among Latinos in the US. Understanding the relationship between increased immigration enforcement and fear of deportation may promote public safety by improving the relationship between the police and Latino communities.
Multivariate ordinal logistic regression analyses of the data found that participants who had a greater fear of deportation reported: (1) less confidence that police would not use excessive force (p<.01); (2) less confidence that police would treat Latinos fairly (p<.05); (3) a lower likelihood of reporting crimes (p<.05); and (4) less confidence that the courts would treat Latinos fairly (p<.01)
Studies on antibiotic resistance in producing microorganisms
No full textThe aminoglycoside phosphotransferase (APH) gene of B. circulans has been cloned in E. coli (CC1105) using the vector pBR322 and has been found to confer antibiotic resistance. The 2.7 kb Sal I fragment containing the APH gene was ligated into the plasmid SLP1.2 and transformed into S. lividans 66 (CL1023), where it was also able to confer antibiotic resistance. When the phosphotransferase activity was assayed in crude extracts of B. circulans, E. coli CC1105 and S. lividans CL1023, using a variety of aminoglycosides as phosphate acceptors, a similar spectrum of activity was found in each strain. Using the methods of Sanger and Messing, the sequence of the 2.7 kb Sal I fragment was determined. An examination of the DNA sequence allowed the protein sequence to be deduced. When the deduced protein sequence was compared with those from S. fradiae, TN5 and TN903, significant homology was found, indicating that all the phosphotransferases may have a common origin. An examination of the sequence either side of the coding region allowed the identification of possible promotors, a ribosome binding site and a terminator. One of the promotors was similar to the σ37 promotors of B. subtilis, showing that the B. circulans APH gene may be under developmental control.</p
Stop messing with old-growth forests
No full textBook review of Nature’s Temples: The Complex World of Old-Growth Forests, by Joan MaloofBook ReviewPublishe
- …
