14 research outputs found
Generation W: From the Young People’s Perspective
No full textTekst razmatra poziciju mlade generacije u postpetooktobarskoj Srbiji, onako kako se ona može iščitati iz empirijskog materijala prikupljenog studijom 'Politika i svakodnevni život : Srbija 1999-2002'. Autorka izdvaja četiri osnovna načina samopozicioniranja mladih, pripadnika generacije R, u odnosu na aktuelni društveni kontekst: 'B92 generacija', 'provincijalci', 'fundamentalisti' i 'gosti', pomoću kojih prati promene u stavovima mladih prema politici, sopstvenom socijalnom i političkom angažmanu, pogledu u budućnost, doživljaju samih sebe i socijalne promene kojoj su svedočili ili u njoj participirali.This paper discusses the position of young people in Serbia after October 5th 2000, as can be inferred from the evidence collected in the study 'Politics and everyday life: Serbia 1999-2002'. The author identifies amongst young people four basic modes of self positioning within the current social context, as described by the following labels: 'B92 generation', 'provincials', 'fundamentalists' and 'guests'. These views encompass politics, their own social and political engagement, views of the future, and their own selves
El rol del profesor de educación fÃsica en la convivencia escolar
No full textTrabajo (licenciado en educación) -- Universidad Mayor (Chile), 2011
Topological defects in a double-mirror quadrupole insulator displace diverging charge
No full textWe show that topological defects in quadrupole insulators do not host
quantized fractional charges, contrary to what their Wannier representation
indicates. In particular, we test the charge quantization hypothesis based on
the Wannier representation of a parametric defect and a disclination. Against
the expectations, we find that the local charge density decays as
with distance, leading to a diverging defect charge. We identify sublattice
symmetry and not higher order topology as the origin of the previously reported
charge quantization.Comment: 7 pages, 3 figure
Exploring mammalian preimplantation development and pluripotency
No full textOur current understanding of how stem cells arise and transition during embryonic development has been limited by analysis tools that have lacked single-cell, whole-genome resolution. This thesis emphasizes the use of novel techniques and cutting-edge technology to better evaluate the biological underpinning of stem cell dynamics in both mouse and human. Development relies on stem cells to establish different lineage potentials from the same starting material. Stem cell populations are produced during embryogenesis at multiple stages, existing in various cellular states. These cells have unique self-renewal properties that allow them to divide without differentiating. Stem cell plasticity becomes more restricted as development progresses. A totipotent stem cell state arises after fertilization once embryo cells can generate exact copies of themselves. Totipotent cells maintain the competency for specification into both embryonic (organism) and extraembryonic (placenta and yolk sack) lineages. Once the mammalian blastocyst is formed, the embryonic lineage is maintained exclusively in epiblast (EPI) cells. Both pre- and postimplantation EPI cells are considered pluripotent stem cells, which lack the capacity for generating extraembryonic tissues but maintain full competency to develop into all embryonic germ lineages. During embryogenesis EPI cells transition through several definable pluripotent states, several of which can be maintained in vitro. This thesis focuses on utilizing better methods for evaluating how well in vitro stem cell culture systems recapitulate endogenous developmental cell types.In Paper I we assessed pre- and postimplantation mouse embryonic stem cells and compared their allelic and transcriptional profiles with developing in vivo cell types. We were able to make unprecedented observations of X chromosome inactivation (XCI) dynamics, elucidating evidence that in vitro mouse XCI does not follow the perceived dogma that preimplantation stem cells express two fully active X chromosomes. By assessing the full length of each X chromosome with allelic resolution we found that XCI is initiated heterogeneously in preimplantation female stem cells with an observable elongated transition between stem cell states. In Paper II we screen two states of human pluripotent stem cells and preimplantation human embryos to define cell surface markers that attempts to effectively separate preimplantation from postimplantation epiblast. The markers provide a sorting method for state conversions. Paper III and IV complement one another in their intent to define the limits of mouse totipotency using transcriptomics and implementation of functional aggregation assays that effectively evaluate lineage specification and commitment. We determined when the first lineage segregation is defined and used an assortment of molecular tools to evaluate embryonic and extraembryonic contribution. This establishes a benchmark for defining totipotency.Together the findings presented in this thesis add significant contribution toward an improved understanding of mammalian embryonic development and stem cell biology.List of scientific papersI. Chen G, SCHELL JP**, Benitez JA, Petropoulos S,Yilmaz M, Reinius B, Alekseenko Z, Shi L, Hedlund E, Lanner F, Sandberg R, Deng Q. (2016). Single-cell analyses of X Chromosome inactivation dynamics and pluripotency during differentiation. Genome Research. 26(10):1342-1354. *First author, **Second author. https://doi.org/10.1101/gr.201954.115 II. Collier AJ*, Panula SP*, SCHELL JP**, Chovanec P, Plaza Reyes A, Petropoulos S, Corcoran AE, Walker R, Douagi I, Lanner F, Rugg-Gunn PJ. (2017). Comprehensive Cell Surface Protein Profiling Identifies Specific Markers of Human Naive and Primed Pluripotent States. Cell Stem Cell. 20(6):874-890.e7. *First author, **Second author. https://doi.org/10.1016/j.stem.2017.02.014 III. Posfai E, Petropoulos S, de Barros F, SCHELL JP, Jurisica I, Sandberg R, Lanner F, Rossant J. (2017). Position- and Hippo signalling-dependent plasticity during lineage segregation in the early mouse embryo. Elife. 6:e22906. https://doi.org/10.7554/eLife.22906 IV. Posfai E*, SCHELL JP*, Adrian Janiszewski*,Isidora R, Murray A, Bradshaw B, Pardon T, Bakkali M, Talon I, Geest N, Kumar P, To S, Petropoulos S, Jurisicova A, Pasque V, Lanner F, Rossant J. (2020). Defining Totipotency Using Criteria of Increasing Stringency. bioRxiv. *First author. [Manuscript] https://doi.org/10.1101/2020.03.02.972893</p
Modulation of N-methyl-D-aspartate receptors in isolated rat heart
No full textConsidering the limited data about the role of N-methyl-D-aspartate receptors (NMDA-R) in cardiovascular system and heart, the aim of the present study was to examine the effects of NMDA and DL-homocysteine thiolacotne (DL-Hcy TLHC), alone and in combinations with glycine, memantine and ifenprodil, in the isolated rat heart. The hearts of Wistar albino rats were retrogradely perfused according to the Langendorff technique at a constant perfusion pressure. The experimental protocol for all experimental groups included the stabilization period, application of estimated substance in duration of 5 minutes, followed by wash-out period in duration of 10 minutes. Using sensor in the left ventricle we registered the next parameters of myocardial function: dp/dt max, dp/dt min, systolic and diastolic left ventricular pressure, and heart rate, and coronary flow (CF) was measured flowmetrically. In the coronary venous effluent spectrophotometrically were estimated following oxidative stress biomarkers: TBARS, NO2-, O2-, and H2O2. NMDA alone did not induce any change in any observed parameter, while DL-Hcy TLHC alone, as well as combined application of NMDA and DL-Hcy TLHC with glycine, induced reduction of most cardiodynamic parameters. Memantine and ifenprodil induced reduction of cardiodynamic parameters and coronary flow, as well as some oxidative stress biomarkers.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author
Creatine phosphate administration in cardiac ischemia-reperfusion injury in rats: focus on differences between preconditioning, perconditioning and postconditioning protocol
No full textThe aim of this study was to examine and compare the influence of preconditioning, perconditioning and postconditioning with creatine phosphate (PCr) on functional recovery and production of prooxidants of isolated rat heart subjected to ex vivo I-R injury on Langendorff apparatus. Wistar albino rats (male, n=40) were divided into 4 groups: control, and groups in which PCr (0.5mmol/l, 5 minutes) was perfused before (Pre group), after (Post group) or during (Per group) ex vivo induced ischemia. PCr application was associated with the great benefits on preserving cardiac contractility (in Pre group 100.96% for +(dP/dt max), 97.61.% for -(dP/dt max), in Per group 96.72% for +(dP/dt max), 95.60.% for -(dP/dt max) and in Post group 143.84% for +(dP/dt max), 104.36% for -(dP/dt max) in relation to the stabilization). In addition, PCr application prevented the rise of pro-oxidative markers during I-R injury in all therapeutic modalities. The most intensive benefits in the current investigation were observed when PCr was applied during the period of ischemia because the lowest fluctuations in the parameters of cardiac function and oxidative stress were observed. Overall, the results of this study highlight PCr-induced cardioprotection with promising prospects for future clinical use.The presentation of the authors' names and (or) special characters in the title of the pdf file of the accepted manuscript may differ slightly from what is displayed on the item page. The information in the pdf file of the accepted manuscript reflects the original submission by the author
Towards a better understanding of human behaviour for improved fisheries science and management
No full text No abstracts are to be cited without prior reference to the author. Conveners: Melania Borit (Norway), Bruce Edmonds (UK), Marloes Kraan (Netherlands).CM 2018/R:114. From fishery management to managing fishing operations using a co-management approach; a SECAS solution. : Larose M., Krug H , Silva H, Slof M. V., Pinho M. R.1CM 2018/R:421. Ecosystem based management; how to make it stick? Matilda ValmanCM 2018/R:543. Do quota markets allow individual fishing companies to adjust quota to catches over time in the Icelandic ITQ system? Maartje Oostdijk, Maria J. Santos, Sveinn Agnarsson, Pamela J. WoodsCM 2018/R:640. Introduction session: Social science aspects for the 21st Century. Melania BoritCM 2018/R:42. Exploring habitat credits to manage the benthic impact in a mixed fishery. J. Batsleer, P. Marchal, S. Vaz, Y. Vermard, A. D. Rijnsdorp, J. J. PoosCM 2018/R:555. Watched but unseen? consequences of technology use for fishery co-management. Hilde M. ToonenCM 2018/R:369. Classifying fishers’ behaviour in the Netherlands: accepting the invitation to fishing styles from Boonstra & Hentati-Sundberg (2016). Amanda Schadeberg, Marloes Kraan, Katell Hamon, Jurgen Batsleer, Eva van den BroekCM 2018/R:384. Introducing a voluntary assessment scheme enabling transition towards a more sustainable fishery. Ellen Pecceu, Lancelot Blondeel, Katrien Verlé, Hans PoletCM 2018/R:60. A field guide for interdisciplinary fisheries scientists. Charlotte Teresa WeberCM 2018/R:89. Modelling fishery behaviour by genetic algorithms. Sylvie GeisendorfCM 2018/R:623. Finding the right spot: recreational sea anglers location choice in the UK. Angela Muench, Kieran Hyder, Adam Brown, Gavin MellorCM 2018/R:183. The evolution of science-industry research cooperation in the Netherlands: crossing boundaries to co-produce knowledge. N.A. Steins, F.J Quirijns, W. van Broekhoven, M.L. KraanCM 2018/R:46. Cooperation and framing effects of economic incentives: the analysis of field experimental data from 240 small-scale fishers in Turkey. Pınar Ertör-AkyazıCM 2018/R:426. Classifying fishers’ behaviour; an invitation to fishing styles. Wiebren Boonstra, Jonas Hentati-SundbergCM 2018/R:85. Collective action as an aid to fisheries management: an example addressing bycatch in the northwest Atlantic mid-water trawl fishery. N.David Bethoney, Bradley P. SchondelmeierCM 2018/R:97. Using normative agents to model policy change scenarios for Icelandic fisheries. Cezara Păstrăv, Jónas Viðarsson, Kristinn Edvardsson, Frank DignumCM 2018/R:649. Data filtering to identify reference fleets to account for abundance of fish species: strengths and weaknesses through three applications: Striped red mullet (Mullus surmulletus), whiting (Merlangius merlangus) and Pollack (Pollachius pollachius). Caill-Milly N.; Bru N.; Lissardy M.; Dutertre M.-A.; Saguet C.; Leaute J.-P.CM 2018/R:153. Social and ecological indicators of fishing community vulnerability and resilience in the United States: an assessment of changing conditions. Lisa L. Colburn, Michael Jepson, Changhua WengCM 2018/R:614. Large pelagic fish tagging programs: how to build synergies with fishermens knowledge backgrounds. Nicolas Goñi, Iñigo Onandia, Josu Santiago, Igor Arregui, MarinaSanturtunCM 2018/R:557. Human behaviour and marine protected areas in the southern ocean. Dr. Ricardo M. RouraCM 2018/R:443. Understanding fisher responses to data collection interventions. Mandy Doddema, Gert Spaargaren, Budy Wiryawan, Simon BushCM 2018/R:164. Triggering the tragedy: the simulated impacts of goal-oriented fisher behaviour on management interventions. Ashleigh Arton, Ernesto Carrella, Jens Koed Madsen, Richard BaileyCM 2018/R:406. How bioeconomic models can help break trade-offs and improve fisheries management evaluations: the North Sea mixed demersal fishery as a case study. Simons Sarah, Ulrich Clara, Döring RalfCM 2018/R:124. Empirical bio-economic modelling of small-scale artisanal fisheries under climate change: a new approach and application to the Senegalese purse-seine fishery. Kira Lancker, Lorena Fricke, Jörn O. SchmidtCM 2018/R:281. Incorporating fisher knowledge into an index of abundance for Atlantic halibut (Hippoglossus hippoglossus) in the Gulf of Maine. Alex Hansell, Greg DeCelles, Steve CadrinCM 2018/R:624. Why do fishermen comply with regulations? The role of preferences. Florian Diekert, Yuanhao Li, Linda Nøstbakken, Andries RichterCM 2018/R:548. Discussion session: Is ‘fishing style’ the way forward to tackle the ‘key source of uncertainty’ in fisheries science: human behaviour? Marloes KraanCM 2018/R:295. Ecosystem-based fisheries management objectives and social, economic, ecological and institutional indicators to measure them. Sara Hornborg, Camilla Novaglio, Jacob Rogers, Beth Fulton, Keith Sainsbury, Ingrid van PuttenCM 2018/R:648. Explicating values to resolve fisheries policy trade-offs and conflicts. Mimi E. LamCM 2018/R:590. Experts as ’honest brokers’? opportunities and limits of scientific advice for policy. Sebstian Linke, Kåre Nolde Nielsen, Petter HolmCM 2018/R:353. Science, participatory approaches and decision support processes in fisheries: sharing experiences and practices from an inter-disciplinary perspective. Claire Macher, Denis Bailly, Marta Ballesteros, Michel Bertignac, Francesco Colloca, Mike Fitzpatrick, Katia Frangoudes, Dorleta Garcia, Marloes Kraan, Rich Little, Simon Mardle, Arantza Murillas, Lionel Pawlowski, Raúl Prellezo, Evelina Sabatella, Nathalie Steins, Olivier Thébaud, Clara UlrichCM 2018/R:245. Public engagement through fisheries visualization. Luz K. MolinaCM 2018/R:169. MPAs and FRAs; acceptance and skepticism by stakeholders. Papadopoulou K. Nadia, Smith Chris J., Pantazi Maria, Karachle Paraskevi K.CM 2018/R:135. Modelling fishers as consumats: a comparison between Homo economicus and Homo psychologicus. Klaas Sys, Jef Van Meensel, Hans PoletCM 2018/R:626. Assessing the impact of the landing obligation at the fleet scale: fisher-scientist collaboration around a complex model. Sigrid Lehuta, Youen Vermard, Marie Savina-Rolland, Morgane Travers-TroletCM 2018/R:161. Social simulation games as vehicles for improved learning about fisheries as socio-ecological complex adaptive systems: the case of the Green Grouper Game. Jørn Weines, Melania Borit, Petter HolmCM 2018/R:629. WKIrish4 – or how I stopped worrying and learned to love the stakeholders. Dave Reid, Francis O’DonnellCM 2018/R:191. Preach water while drinking wine? time preferences of fishery managers and implications for management. Rudi Voss, Jörn O. Schmidt, Max T. Stöven, Martin F. QuaasCM 2018/R:39. The importance of choice architecture for the formulation of fisheries management rules. Paul J B Hart, Sarah B M KraakCM 2018/R:359. Cross-scale interactions between local and regional fisheries. Emilie Lindkvist, Andrew Frederick Johnson, Blanca Gonzalez García-Món, Alfredo Girón-Nava, Octavio Aburto-Oropeza, Maja SchlüteCM 2018/R:35. The “science dimension” of fisheries management. Lia ní Aodha, Bruce EdmondsCM 2018/R:225. When collaborative planning becomes dysfunctional. Gunnar SanderCM 2018/R:663. European public understanding of fisheries; its importance and impacts. Cristina Pita, Isidora Katara, Tavis PottsCM 2018/R:168. Improving understanding of the influence of Ireland’s monthly quota system on discarding behaviour and how such behaviour might change under the Landing Obligation. Julia Calderwood, Dave ReidCM 2018/R:413. Understanding adaptation strategies of small-scale fishermen in the face of abrupt environmental disturbances. Lotta C. Kluger, Sophia Kochalski, Philipp Gorris, Matthias WolffCM 2018/R:116. Narratives of ecological change: understanding artisanal fishers' responses to the governance of marine resources in a Natura site. C. Mouro, T. Santos e P. CastroCM 2018/R:562. Network analysis in social-ecological systems research. Gorris, P., Kluger, L.C., Kochalski, S., Mueller, M.S., Romagnoni, G.CM 2018/R:373. Coping with environmental challenges: archetypes and emergence of adaptive strategies. Kochalski, S., Harrison, H.L., Arlinghaus, R.CM 2018/R:143. A framework for building value-based agent models of fishers. Samaneh Heidari, Frank DignumCM 2018/R:182. A social network analysis of stakeholders involved in the management of western Baltic cod. Heike Schwermer, Christian MöllmannCM 2018/R:101. Co-authorship network analysis for fisheries indexed publications. Shaheen Syed, Lia ní AodhaCM2018/R:598. Scoping Small-Scale Fisheries: Opportunities of ICES’ Integrated Ecosystem Assessment approach for a comprehensive approach of Azores Artisanal Fisheries’ social components. Ana Fraga</p
EFFICENCY OF ATORVASTATIN AND SIMVASTATIN IN IMPROVING OF CARDIAC FUNCTION DURING THE DIFFERENT DEGREE OF HYPERHOMOCYSTEINEMIA
No full textThe aim of this study was to assess the impact of atorvastatin and simvastatin on myocardial contractility during the different degrees of hyperhomocysteinemia (HHcy) in rats. Study was conducted on adult male Wistar albino rats (n = 90; 4 weeks old; 100 ± 15 g body mass) in which HHcy was achieved by dietary manipulation. Animals were exposed to pharmacology treatment with atorvastatin in dose of 3 mg/kg per day i.p. or simvastatin in dose of 5 mg/kg per day i.p. at the same time every day, according to equivalent therapeutic doses of these statins (10 mg atorvastatin = 20 mg simvastatin). After the dietary manipulation and pharmacological treatment and confirmation of HHcy, all animals were sacrificed, hearts were isolated, and cardiac function was tested according to the Langendorff technique. Size of recovery of maximum rate of left ventricular development (dp/dtmax), minimum rate of left ventricular development (dp/dtmin), systolic left ventricular development, diastolic left ventricular development, heart rate, and coronary flow at the 40, 60, 80, 100, and 120 cmH2O coronary perfusion pressure were measured in state of physiological condition (homocysteine less than 15 μmol/L), mild HHcy, and moderate HHcy. Atorvastatin treatment significantly attenuated homocysteine-induced impairment of myocyte contractility and dominantly decreased dp/dtmax, dp/dtmin, and heart rate and induced greater changes in systolic left ventricular development compared with simvastatin. Treatment with atorvastatin seems able to revert systolic abnormalities and improve contractility during the different degrees of HHcy.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author
The influences of chokeberry extract supplementation on redox status and body composition in handball players during competition phase
No full textThe aim of our study was to investigate the influence of 12 weeks of consumption of chokeberry extract on redox status, body composition, lipid profile, and biochemical parameters in active handball players. The study included 16 handball players aged 16–24 years (20.26 ± 2.86 years). Every morning before training, players received 30 mL of liquid chokeberry extract for 12 weeks during the regular competition season. The research consisted of morphofunctional and biochemical testing, which was performed at three points (at the beginning of the study and at 6 and 12 weeks after extract consumption). After the chokeberry extract treatment, we observed significant changes in three main aspects. The 12 week supplementation with chokeberry extract decreased the levels of prooxidants (TBARS and nitrites) and increased catalase activity. Analyzing the dynamic of body composition showed a decrease in body fat (9.4 ± 0.5 vs. 7.3 ± 0.6 kg) as well as its percent in a body (11.4 ± 0.4% vs. 8.8 ± 0.4%). On the other hand, the analysis showed an increase of high-density lipoprotein (1.3 ± 0.3 vs. 1.6 ± 0.2 mmol/L) and hemoglobin (144.4 ± 11.7 vs. 151.7 ± 9.9 g/L) after 6 weeks of treatment. At the same time, a decrease in leukocytes (7.2 × 109 ± 2.8 vs. 6.5 ± 1.2 × 109/L) and an increase in red blood cells count (4.9 ± 0.4 × 109 vs. 5.5 ± 0.5 × 109/L) were observed. Overall, these results emphatically show that the use of chokeberry extract dietary supplement induced a wide range of beneficial effects in the examined group of athletes.The presentation of the authors' names and (or) special characters in the title of the pdf file of the accepted manuscript may differ slightly from what is displayed on the item page. The information in the pdf file of the accepted manuscript reflects the original submission by the author
OPA1 and disease-causing mutants perturb mitochondrial nucleoid distribution
No full textThe copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.Optic atrophy protein 1 (OPA1) mediates inner mitochondrial membrane (IMM) fusion and cristae organization. Mutations in OPA1 cause autosomal dominant optic atrophy (ADOA), a leading cause of blindness. Cells from ADOA patients show impaired mitochondrial fusion, cristae structure, bioenergetic function, and mitochondrial DNA (mtDNA) integrity. The mtDNA encodes electron transport chain subunits and is packaged into nucleoids spread within the mitochondrial population. Nucleoids interact with the IMM, and their distribution is tightly linked to mitochondrial fusion and cristae shaping. Yet, little is known about the physio-pathological relevance of nucleoid distribution. We studied the effect of OPA1 and ADOA-associated mutants on nucleoid distribution using high-resolution confocal microscopy. We applied a novel model incorporating the mitochondrial context, separating nucleoid distribution into the array in the mitochondrial population and intramitochondrial longitudinal distribution. Opa1-null cells showed decreased mtDNA levels and nucleoid abundance. Also, loss of Opa1 lead to an altered distribution of nucleoids in the mitochondrial population, loss of cristae periodicity, and altered nucleoids to cristae proximity partly rescued by OPA1 isoform 1. Overexpression of WT OPA1 or ADOA-causing mutants c.870+5G>A or c.2713C>T in WT cells, showed perturbed nucleoid array in the mitochondria population associated with cristae disorganization. Opa1-null and cells overexpressing ADOA mutants accumulated mitochondria without nucleoids. Interestingly, intramitochondrial nucleoid distribution was only altered in Opa1-null cells. Altogether, our results highlight the relevance of OPA1 in nucleoid distribution in the mitochondrial landscape and at a single-organelle level and shed light on new components of ADOA etiology. The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission
