672 research outputs found
Dafnis y Cloe: Una novela griega de la Antigüedad tardía
No full textFil: Barranco, María Isabel. Universidad Nacional de La Plata
Diseño y Análisis del Boeing 747:Evolución, Dimensionado, Actuaciones y Rentabilidad.
No full textLópez-Barranco Pérez, Isabel; director de proyecto: Maroto Villar, Ignacio2024-2025Grado en Ingeniería AeroespacialEscuela de Ingeniería, Arquitectura y Diseñ
Diseño y Análisis del Boeing 747:Evolución, Dimensionado, Actuaciones y Rentabilidad.
No full textLópez-Barranco Pérez, Isabel; director de proyecto: Maroto Villar, Ignacio2024-2025Grado en Ingeniería AeroespacialEscuela de Ingeniería, Arquitectura y Diseñ
"Proyecto por el Barranco Badajoz"
No full textA research project developed by a cluster of the University of Udine coordinated by the author between 2005 and 2006 and presented in Tenerife at the Seminario Internacional de Arquitectura: Proyectar el paysaje. Territorios en Transformacion - Barranco Badajoz de Guimar, Tenerife. The seminar had gathered 15 European schools of Architecture and the proposed topics had developed the contrasting needs of opposed reasons and economies simultaneously acting in an extraordinary valuable landscape. The re-discovery of the inherent values of unearthing, of the “ungrounding”, of the interlace thought as a micro-climatic “overhaul” founded the “Weather Projects” of the proposal shown during the final symposium in June 2006
Comparative proteomics of extracellular vesicles subsets isolated from pig seminal plasma
No full textDetection and immunophenotypic analysis of seminal extracellular vesicles by flow cytometry
No full textPig seminal extracellular vesicles (sEVs) load cytokines showing quantitative differences between sEV subsets
No full textEl Tlacuache Núm. 612 (2014). 612 Año 13 (2014) marzo. El Tlacuache
No full textRitualidades perturbadas. Dos casos de injerencia externa dentro de la religiosidad nahua en torno al Culto a la Montaña por José Antonio Sampayo Barranco
SPARTEVs. Characterization of seminal extracellular vesicles subsets used for supplementing in vitro maturation medium of porcine oocytes
No full textResearch on extracellular vesicles (EVs), membrane-delimited nanoparticles (30-to-1000 nm) released by functional cells is currently a challenge for scientific community since they act as essential messengers in pathological and physiological processes, including those related to reproduction. Based on differences in size and biogenesis pathways, EVs released by healthy living cells are classically categorized into exosomes (small EVs) and microvesicles (large EVs). These EVs has been isolated from all reproductive fluids, including seminal plasma (SP), a complex and heterogeneous fluid composed mainly by secretions from accessory sexual glands that accompanies sperm during and after ejaculation. Bearing in mind that SP is beneficial for sperm-oocyte interaction and embryo development and that it contains EVs, which are involved in several sperm functions, the hypothesis of SPARTEVs project was that seminal EVs may play a key role in the in the successful development of the above-mentioned reproductive events. This data set contains the raw data of the characterization of EVs subsets (small (S-EVs) and large (L-EVs)) isolated from pig SP (by a combination of centrifugation, size-exclusion chromatography and ultrafiltration) and used for supplementing in vitro maturation medium of porcine oocytes. The characterization was conducted following MISEV2018 guidelines, and this data set included the raw data obtained from dynamic scattering, nano tracking particle and flow cytometry analyses
SPARTEVs. Gene expression data of cumulus cells in response to seminal extracellular vesicles subsets during in vitro maturation of porcine oocytes
No full textExtracellular vesicles (EVs), nano-sized (30-to-1000 nm) membrane vesicles secreted by all functional cells, are essential intercellular mediators, modulating several physiological and pathological processes. According to their size and biogenic pathway, EVs released by living cells are classically categorized into exosomes (small EVs) and microvesicles (large EVs). EVs has been isolated from all biofluids, including seminal plasma (SP), a fluid composed by secretions from accessory sex glands that accompanies sperm during and after ejaculation. Seminal EVs modulate several sperm physiological processes, and female reproductive tract immune environment, which is required for successful embryo development. Evidence supports that SP may also affect ovarian function. This study evaluated the potential effect of supplementing with two SP-EVs subsets (small (S-EVs) and large (L-EVs)) during in vitro maturation (IVM) of porcine oocytes on gene expression of cumulus cells. Four pathways were investigated using a total of nine genes: i) cell apoptosis using B-cell lymphoma 2 (BCL2) and BCL2 Associated X (BAX); ii) cell proliferation using Cyclin B1 (CCNB1); iii) oocyte maturation through Connexin 43 (CX43), Hyaluronan Synthase 2 (HAS2) and Stearoyl-CoA desaturase 1 (SCD1); and iv) steroidogenesis using Cytochrome P450 Family 11 Subfamily A Member 1 (CYP11A1), Hydroxy-Delta-5-Steroid Dehydrogenase 3 Beta (HSD3B1) and aromatase (CYP19A1). All genes, including housekeeping 60S Ribosomal Protein L18 (RPL19), were selected based on previous literature. The mRNA levels were assessed by quantitative real-time PCR
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