1,721,090 research outputs found
Thymosin fraction 5 re-evaluated after 35 years by high-resolution mass spectrometry.
No full textOBJECTIVES:
We reevaluated a lyophilized sample of thymosin fraction 5, stored for 37 years at room temperature, by high-resolution mass spectrometry in terms of stability and yet uncharacterized polypeptides that could be biological important substances.
METHODS:
A top-down proteomic platform based on high-performance liquid chromatography (HPLC) coupled to high-resolution LTQ-Orbitrap mass spectrometry (MS) was applied to molecular characterization of polypeptides present in thymosin fraction 5.
RESULTS:
We detected more than 100 monoisotopic masses corresponding to thymosin β4 and truncated forms of ubiquitin, prothymosin α, thymosin β4, and thymosin β9. Additionally, we discovered a new polypeptide present in thymosin fraction 5 and identified it as intact SH3 domain-binding glutamic acid-rich-like protein 3.
CONCLUSION:
In spite of the well-known proteolytic processes inherent to the preparation of thymosin fraction 5, still uncharacterized polypeptides as well as truncated forms of already well-known thymosins are present in fraction 5 after long-term storage. Therefore, continuing characterization of thymosin fraction 5 is even nowadays highly promising
Genitorialità migrante: un’esperienza di formazione interculturale con madri immigrate a Napoli
No full textOur contribution is as theoretical and methodological reflection on the specific phenomenon
of new immigrant, interfaith families. Some questions were asked about this matter:
what happens within these families? Are identity and origin of each parent important
predictors of children’s educational models? Which of the two lineages prevails: motherhood
or fatherhood? What paths of parenting? Are there differences of gender? These
questions will have only partial answers in this reflection; however, our aim is to develop
a pedagogical model of the phenomenon, through the description of an educational
practice. In terms of education quality, the first question that arises is to bring together these multiplicities and differences within an educational model, whose characteristics
of stability, consistency and functionality may help to avoid the risk of confusion and
disorientation in subjects who live in real-life complex situations
Shear bond between existing concrete and high-strength fiber reinforced overlay: Influence of substrate strength and interface roughness
No full textBonded concrete overlays are widely used for strengthening and repair of concrete members. The aim of a concrete repair is to restore the load-carrying capacity and stiffness of a concrete member, and to extend the service life of a structure. The bond between substrate and overlay is one of the main factors concerning the serviceability of a composite member consisting of concrete of different ages. This paper aims at better evaluating, through an extensive experimental program, the shear bond strength between existing concrete and a subsequently applied fiber reinforced overlay. The main purposes of this test program are to study the influence of the substrate strength and of the substrate surface roughness on the interface bond strength between a substrate and overlay concrete. A refinement of a novel bond test, proposed by some of the authors of this paper, is also implemented. Results indicate that there is an increase in the bond strength with an increase in the substrate compressive strength. On the other hand, the results suggest that, for the substrate strength classes investigated, the surface roughness does not influence the interface bond strength
Capillary electrophoresis-mass spectrometry for the analysis of amino acids
No full textIn this review, the recent contribution of CE-MS technology to the analysis of amino acids, as well as the advantages of the hyphenation and the technologies involved in the instrumental coupling are reported. Different sections are dedicated to the recent contributions of CE-MS to the analysis of protein amino acids and their post-translational modifications, such as phosphorylation and sulfation. CE-MS analysis of some amino acid derivatives, such as the free methylated-derivatives of arginine is also discussed. A section is specifically devoted to the CE-MS applications in the field of chiral separation of D- and L-amino acid enantiomers
Capillary electrophoresis-mass spectrometry: Recent trends in clinical proteomics
No full textThe increasing attention now paid to the elucidation of human proteome strengthened the development of analytical instruments able to provide reliable proteins and peptides quantitation and characterization in biological fluids and tissues. Emerging from proteomics, clinical proteomics exclusively considers its biomedical applications. It evaluates, often by high-throughput comparative platforms, the protein and peptide variations in body fluids, cells and tissues under different physiological and pathological conditions with the aim of discovering disease biomarkers. Among the available analytical methodologies, mass spectrometry in coupling with liquid chromatography or capillary electrophoresis demonstrated to be the eligible technique for protein detection and identification. This review summarizes the most recent applications of capillary electrophoresis-mass spectrometry to clinical proteomics, focusing on capillary zone electrophoresis separation mode and ESI and MALDI ionizations, which are the most frequently applied capillary electrophoresis-mass spectrometry hyphenated techniques
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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