7,065 research outputs found

    TACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation

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    The interaction between TACC3 (transforming acidic coiled coil protein 3) and the microtubule polymerase ch-TOG (colonic, hepatic tumor overexpressed gene) is evolutionarily conserved. Loading of TACC3–ch-TOG onto spindle microtubules requires the phosphorylation of TACC3 by Aurora-A kinase and the subsequent interaction of TACC3 with clathrin to form a microtubule binding surface. Whether there is a pool of TACC3–ch-TOG that is independent of clathrin in human cells, and what is the function of this pool, are open questions. Here, we report that TACC3 is recruited to the plus-ends of microtubules by its association with ch-TOG and that this pool is independent of phosphorylation and binding to clathrin. The plus-end binding of TACC3–ch-TOG persists in interphase and we propose that one cellular function of TACC3–ch-TOG is to modulate cell migration. We also describe the distinct subcellular pools of TACC3, ch-TOG and clathrin. TACC3 is often described as a centrosomal protein, but we show that there is no significant population of TACC3 at centrosomes. The delineation of distinct protein pools reveals a simplified view of how these proteins are organized and controlled by post-translational modification

    J. V. G. Mills (translated by) : Hsing-ch 'a sheng-lan : The Overall Survey of the Star Raft by Fei Hsin

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    Manguin Pierre-Yves. J. V. G. Mills (translated by) : Hsing-ch 'a sheng-lan : The Overall Survey of the Star Raft by Fei Hsin. In: Bulletin de l'Ecole française d'Extrême-Orient. Tome 84, 1997. pp. 441-442

    A Note on the Poem "Shu Chiang-hsi Tsao-k'ou Pi" Written by Hsin Ch'i-chi to the Melody P'u-sa-man

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    The most detailed and reliable study of the tz'u style poetry of Hsin Ch'i-chi (1140-1207) is the Chronological Study of Chia-hsilan's Tz'u 稼軒詞編年箋注 by Teng Kuang-ming 鄧廣銘, Shanghai, 1957. The author disagrees with Professor Teng's interpretation of the poem mentioned in the title of this article, particularly the first part of the poem. The author believes that the poet, when he composed this poem, had in mind the fact that Empress Dowager Meng 孟, the consort of Emperor Che-tsung 哲宗, was pursued by the Khitan armies in 1129 in the region of Tsao-k'ou in Kiangsi and only barely escaped with her life, basing his assumption upon the account of this incident contained in the Hao-lin yü-lu 鶴林玉露 (preface dated 1248). Professor Teng rejects this account, however, because no mention is made of the incident in the dynastic histories. The author, for further support of his contention, cites a record of the incident found in ch. 35 of the San-eh'ao pei-meng huipien 三朝北盟會編 compiled by Hsü Meng-hsin 徐夢莘

    Les diminutifs basques avec ch

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    Se presentan formaciones similares a los diminutivos vascos con "ch" en España y América latina. Se dan ejemplosThe author introduces similar formations to the Basque diminutive "ch" in Spain and Latin America. Examples are provide

    COUPLING OF THE C-H STRETCH TO LARGE-AMPLITUDE TORSION AND INVERSION MOTIONS: COMPARISON OF CH3{_3}CH2{_2}.^{.}, CH3{_3}OH2{_2}+^{+} AND CH3{_3}NH2{_2}

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    Author Institution: Department of Polymer Science and Department of Chemistry, The University of Akron; Department of Chemistry, The University of Akron, OH 44325In each of the title molecules, torsional and inversion tunneling occurs between six equivalent minima. Coupling of these degrees of freedom to the CH stretch occurs via variation of the C-H stretching force constants as a function of the torsional (α\alpha) and inversion (τ\tau) angles. Maps of the couplings have been computed at the MP2/6-311++G(3df,2p) level. Both the single bond CH stretch force constants and the bilinear couplings between CH bonds are presented as a function of α\alpha and τ\tau. Although the torsional barriers differ by more than a factor of 20, the torsion-inversion-vibration coupling patterns are very similar for CH3{_3}NH2{_2} and CH3{_3}CH2{_2}.^{.}. On the other hand, the torsion-inversion-vibration coupling in the charged species CH3{_3}OH2{_2}+^{+} is much weaker

    Reply to the Ch. Lagrange’s note

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    The author answers to a Ch. Lagrange’s note who refutes his theory on the diurnal nutation.L’auteur répond à une note de Ch. Lagrange qui réfute sa théorie sur la nutation diurne

    NATURE OF TORSION-INVERSION COUPLING IN CH3_3NH2_2, CH3_3OH2+_2^+ AND CH3_3CH2_2\cdot

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    Author Institution: Department of Chemistry, The University of Akron, OH; 44325-3601Two-dimensional torsion-inversion surfaces for methylamine, protonated methanol and ethyl radical were calculated and fit to a function containing a polynomial in the inversion angle(τ\tau) and trigonometric functions of the torsional angle(α\alpha). Calculations were done at the B3LYP, MP2, and CCSD(T) levels with the 6-311++G(d,p) and 6-311++G(3df, 2p) basis sets and partial optimization. CH3_3NH2_2, CH3_3OH2+_2^+ and CH3_3CH2_2\cdot have G12_{12} symmetry with 6-equivalent minima which are located by the various calculations at inversion angles 6.5 to 11; 42 to 45.5 and 52.5 to 55 degrees respectively on either side of planar. The three molecules have very different barriers to inversion ranging from no barrier for CH3_3CH2_2\cdot to 838 cm1^{-1} for CH3_3OH2+_2^+ to 1837 cm1^{-1} for CH3_3NH2_2. The dominant torsion-inversion coupling term in all cases has the form τcos3α\tau{cos3}\alpha

    Note about Mr. Ch. Lagrange’s recent communication

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    The author establishes a note about Mr. Ch. Lagrange’s communication on the Eulerian nutation period.L’auteur établit une note au sujet d’une communication de Ch. Lagrande sur la période de la nutation eulérienne

    Convenient two-dimensional model for design of fuel channels for proton exchange membrane fuel cells

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    A theoretical, two-dimensional, along-the-channel model has been developed to design fuel channels for proton exchange membrane (PEM) fuel cells. This has been implemented by solving the resultant ordinary differential equation with a straightforward shooting computational scheme. With such a design tool, an analysis can be made of the effects due to some operation and design parameters, such as inlet velocity, inlet pressure, catalyst activity, height of channel, and porosity of gas-diffusion layer to obtain a fuel cell with high performance. Present results indicate that there is always a trade-off between higher power density and higher efficiency of the fuel cell. Namely, a design for higher power density (a better performance) is always accompanied with a higher fuel efficiency (or a larger fuel consumption rate and a higher fuel cost), and vice versa. When some relevant physical parameters are determined experimentally and applied in the present model, a quantitative design for a fuel cell of high efficiency or performance is feasible. uncorrected proof. journal of power sources xxx (2003) xxx–xxx. 3. convenient two-dimensional model for design of fuel channels. 4. for proton exchange membrane fuel cells. 5. falin chen. a.?. . ying-zhi wen. a. . hsin-sen ch
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