4,391 research outputs found
Multiple functions of LIM domain-binding CLIM/NLI/Ldb cofactors during zebrafish development
No full textThe crucial involvement of CLIM/NLI/Ldb cofactors for the exertion of the biological activity of LIM homeodomain transcription factors (LIM-HD) has been demonstrated. In this paper we show that CLIM cofactors are widely expressed during zebrafish development with high protein levels in specific neuronal cell types where LIM-HD proteins of the Isl class are synthesized. The overexpression of a dominant-negative CLIM molecule (DN-CLIM) that contains the LIM interaction domain (LID) during early developmental stages of zebrafish embryos results in an impairment of eye and midbrain-hindbrain boundary (MHB) development and disturbances in the formation of the anterior midline. On a cellular level we show that the outgrowth of peripheral but not central axons from Rohon Beard (RB) and trigeminal sensory neurons is inhibited by DN-CLIM overexpression. We demonstrate a further critical role of CLIM cofactors for axonal outgrowth of motor neurons. Additionally, DN-CLIM overexpression causes an increase of Isl-protein expression levels in specific neuronal cell types, likely due to a protection of the DN-CLIM/LIM-HD complex from proteasomal degradation. Our results demonstrate multiple roles of the CLIM cofactor family for the development of entire organs, axonal outgrowth of specific neurons and protein expression levels
Yves-Heng Lim
No full textYves-Heng Lim est enseignant-chercheur au Département d’Etudes de Sécurité et de Criminologie de l’Université Macquarie, Sydney. Il est l’auteur de China’s Naval Power: An Offensive Realist Approach (Ashgate, 2014). Yves-Heng Lim is a lecturer at the Department of Security Studies and Criminology, Macquarie University. He is the author of China’s Naval Power: An Offensive Realist Approach (Ashgate, 2014)
The critical power function is dependent on the duration of the predictive exercise tests chosen
No full textThe linear relationship between work accomplished (W(lim)) and time to exhaustion (t(lim)) can be described by the equation: W(lim) = a + CP x t(lim). Critical power (CP) is the slope of this line and is thought to represent a maximum rate of ATP synthesis without exhaustion, presumably an inherent characteristic of the aerobic energy system. The present investigation determined whether the choice of predictive tests would elicit significant differences in the estimated CP. Ten female physical education students completed, in random order and on consecutive days, five all-out predictive tests at preselected constant-power outputs. Predictive tests were performed on an electrically-braked cycle ergometer and power loadings were individually chosen so as to induce fatigue within approximately 1-10 mins. CP was derived by fitting the linear W(lim)-t(lim) regression and calculated three ways: 1) using the first, third and fifth W(lim)-t(lim) coordinates (I135), 2) using coordinates from the three highest power outputs (I123; mean t(lim) = 68-193 s) and 3) using coordinates from the lowest power outputs (I345; mean t(lim) = 193-485 s). Repeated measures ANOVA revealed that CPI123 (201.0+/-37.9W) > CPI135 (176.1+/-27.6W) > CPI345 (164.0+/-22.8W) (P<0.05). When the three sets of data were used to fit the hyperbolic Power-t(lim) regression, statistically significant differences between each CP were also found (P<0.05). The shorter the predictive trials, the greater the slope of the W(lim)-t(lim) regression; possibly because of the greater influence of 'aerobic inertia' on these trials. This may explain why CP has failed to represent a maximal, sustainable work rate. The present findings suggest that if CP is to represent the highest power output that an individual can maintain "for a very long time without fatigue" then CP should be calculated over a range of predictive tests in which the influence of aerobic inertia is minimised
Professional attachment report [with] Chio Lim & Associates.
No full textThis report serves as a summary of the professional attachment. Besides touching on author experiences working with Chio Lim & Associates (CLA), it wil also touch on other issues before, during and after the program
Lim-inf convergence and its compactness
No full textAbstract- We describe the Mizar formalization of the proof of compactness of lim-inf convergence given in [W33] according to [CCL]. Lim-inf convergence formalized in [W28] is a Moore-Smith convergence investigated in [Y6] and involves the concept of nets. The proof is based on the equivalence of two approaches to convergence in topological spaces: filter convergence and Moore-Smith (net) convergence. The equivalence is worked out in [Y19] and different characterizations of compactness are also given there. These efforts are a continuation of the international project of formalizing the theory of continuous lattices headed by the first author
“Survival – to keep writing”: An interview with Shirley Geok-lin Lim
No full textIn this e-mail interview conducted in 2016, author and scholar Shirley Geok-lin Lim addresses the changing social and political conditions in the United States. Lim discusses the affective relationship between aesthetics and politics in her work, the anxiety of multilingual stylistics, and the in-between nature of the transnation. She also reflects on the academic marginalization she has experienced as a result of her immigrant designation and subjectivity, as well as the indirect influence of China and Chineseness on her writing. Commenting on her memoir Among the White Moon Faces, Lim notes the difficulty of titling, and addresses the impact of anglophone literature upon her during her colonial Malaysian upbringing
Majelis Ta’lim Sebagai Alternatif Pusat Pendidikan Islam (Studi Kasus pada Majelis Ta’lim Se Kecamatan Natar Lampung Selatan)
No full textIn the public, living we see routine activities what is done. The routine activities are religious and non religious. Usually, religious activities are done in majelis ta’lim. Majelis ta’lim that grow in the community can to became alternative for central of islamic education if the managed well. Therefore, the author want to discuss about this issue with expectation to know of factors that cause the failure religious building in majelis ta’lim especially in Natar, so to know activities that required to done so as really became alternative for central of Islamic education. This research is quantitative research with collecting data from majelis ta’lim which became this research object. The collecting data is done with instruments: as observation, interview, and documentation. From this research, we can know that style and methode is done by the teacher is principal key to success in majelis ta’lim. Therefore, the inovation is required in majelis ta’lim activities so that can interest of more pilgrims and make him the alternative for central of Islamic education
Interactions between LHX3-And ISL1-family LIM-homeodomain transcription factors are conserved in Caenorhabditis elegans
No full text© 2017 The Author(s). LIM-Homeodomain (LIM-HD) transcription factors are highly conserved in animals where they are thought to act in a transcriptional 'LIM code' that specifies cell types, particularly in the central nervous system. In chick and mammals the interaction between two LIM-HD proteins, LHX3 and Islet1 (ISL1), is essential for the development of motor neurons. Using yeast two-hybrid analysis we showed that the Caenorhabditis elegans orthologs of LHX3 and ISL1, CEH-14 and LIM-7 can physically interact. Structural characterisation of a complex comprising the LIM domains from CEH-14 and a LIM-interaction domain from LIM-7 showed that these nematode proteins assemble to form a structure that closely resembles that of their vertebrate counterparts. However, mutagenic analysis across the interface indicates some differences in the mechanisms of binding. We also demonstrate, using fluorescent reporter constructs, that the two C. elegans proteins are co-expressed in a small subset of neurons. These data show that the propensity for LHX3 and Islet proteins to interact is conserved from C. elegans to mammals, raising the possibility that orthologous cell specific LIM-HD-containing transcription factor complexes play similar roles in the development of neuronal cells across diverse species
“Survival -- to keep writing”: an interview with Shirley Geok-lin Lim
No full textIn this e-mail interview conducted in 2016, author and scholar Shirley Geok-lin Lim addresses the changing social and political conditions in the United States. Lim discusses the affective relationship between aesthetics and politics in her work, the anxiety of multilingual stylistics, and the in-between nature of the transnation. She also reflects on the academic marginalization she has experienced as a result of her immigrant designation and subjectivity, as well as the indirect influence of China and Chineseness on her writing. Commenting on her memoir Among the White Moon Faces, Lim notes the difficulty of titling, and addresses the impact of anglophone literature upon her during her colonial Malaysian upbringing
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