1,724,237 research outputs found
Phototactic Behavior of Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae)
No full textChen, Zhen, Li, Hong-Mei, Zhou, Cheng-Li (2020): Phototactic Behavior of Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae). The Coleopterists Bulletin 74 (3): 513-522, DOI: 10.1649/0010-065X-74.3.513, URL: http://dx.doi.org/10.1649/0010-065x-74.3.51
The Influenza M2 Ectodomain Regulates the Conformational Equilibria of the Transmembrane Proton Channel: Insights from Solid-State Nuclear Magnetic Resonance
No full textThe influenza M2 protein is the target of the amantadine family of antiviral drugs, and its transmembrane (TM) domain structure and dynamics have been extensively studied. However, little is known about the structure of the highly conserved N-terminal ectodomain, which contains epitopes targeted by influenza vaccines. In this study, we synthesized an M2 construct containing the N-terminal ectodomain and the TM domain, to understand the site-specific conformation and dynamics of the ectodomain and to investigate the effect of the ectodomain on the TM structure. We incorporated ¹³C- and ¹⁵N-labeled residues into both domains and measured their chemical shifts and line widths using solid-state nuclear magnetic resonance. The data indicate that the entire ectodomain is unstructured and dynamic, but the motion is slower for residues closer to the TM domain. ¹³C line shapes indicate that this ecto-TM construct undergoes fast uniaxial rotational diffusion, like the isolated TM peptide, but drug binding increases the motional rates of the TM helix while slowing the local motion of the ectodomain residues that are close to the TM domain. Moreover, ¹³C and ¹⁵N chemical shifts indicate that the ectodomain shifts the conformational equilibria of the TM residues toward the drug-bound state even in the absence of amantadine, thus providing a molecular structural basis for the lower inhibitory concentration of full-length M2 compared to that of the ectodomain-truncated M2. We propose that this conformational selection may result from electrostatic repulsion between negatively charged ectodomain residues in the tetrameric protein. Together with the recent study of the M2 cytoplasmic domain, these results show that intrinsically disordered extramembrane domains in membrane proteins can regulate the functionally relevant conformation and dynamics of the structurally ordered TM domains.National Institutes of Health (U.S.) (Grant GM088204
Tercera/cuarta lengua mundial/extranjera: Chino A1
No full textDoble Grado en Psicología y Criminología, Doble Grado en Relaciones Internacionales y en Traducción e Interpretación, Grado en Traducción e Interpretación, Grado en Administración y Dirección de Empresas (E-2), Grado en Administración y Dirección de Empresas Mención Internacional (E-4), Grado en Ingeniería de Tecnologías de Telecomunicación, Grado en Ingeniería de Tecnologías Industriales, Grado en Relaciones Internacionales y Grado en Comun. Internal. - Bachelor in Global Communicatio
Solid-state NMR investigations of cellulose structure and interactions with matrix polysaccharides in plant primary cell walls
Full text linkUntil recently, the 3D architecture of plant cell walls was poorly understood due to the lack of high-resolution techniques for characterizing the molecular structure, dynamics, and intermolecular interactions of the wall polysaccharides in these insoluble biomolecular mixtures. We introduced multidimensional solid-state NMR (SSNMR) spectroscopy, coupled with [superscript 13]C labelling of whole plants, to determine the spatial arrangements of macromolecules in near-native plant cell walls. Here we review key evidence from 2D and 3D correlation NMR spectra that show relatively few cellulose–hemicellulose cross peaks but many cellulose–pectin cross peaks, indicating that cellulose microfibrils are not extensively coated by hemicellulose and all three major polysaccharides exist in a single network rather than two separate networks as previously proposed. The number of glucan chains in the primary-wall cellulose microfibrils has been under active debate recently. We show detailed analysis of quantitative [superscript 13]C SSNMR spectra of cellulose in various wild-type (WT) and mutant Arabidopsis and Brachypodium primary cell walls, which consistently indicate that primary-wall cellulose microfibrils contain at least 24 glucan chains.United States. Dept. of Energy. Office of Basic Energy Sciences (Center for Lignocellulose Structure and Formation Award # DE-SC0001090
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Student Project: Replication of Cheng-Ming Jiang, Hong-Mei Sun, Long-Fei Zhu, Lei Zhao, Hong-Zhi Liu, Hong-Yue Sun (2017, JDM, Study 2)
No full textThis project is an independent replication of Cheng-Ming Jiang., Hong-Mei Sun., Long-Fei Zhu., Lei Zhao., Hong-Zhi Liu., Hong-Yue Sun. (2017). Better is worse, worse is better: Reexamination of violations of dominance in intertemporal choice. Judgment and Decision Making ,12(3), 253-259., conducted as part of the Hagen Cumulative Science Project
Student Project: Replication of Cheng-Ming Jiang, Hong-Mei Sun, Long-Fei Zhu, Lei Zhao, Hong-Zhi Liu, Hong-Yue Sun (2017, JDM, Study 2)
No full textThis project is an independent replication of Cheng-Ming Jiang., Hong-Mei Sun., Long-Fei Zhu., Lei Zhao., Hong-Zhi Liu., Hong-Yue Sun. (2017). Better is worse, worse is better: Reexamination of violations of dominance in intertemporal choice. Judgment and Decision Making ,12(3), 253-259., conducted as part of the Hagen Cumulative Science Project
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