1,727,789 research outputs found
Jun-ho Choi
No full text학위논문(석사)--아주대학교 일반대학원 :심리학과,2017. 2알코올 사용장애는 심리학적 관점에서 만성적인 자기조절실패로 개념화되고 있는 행동장애로, 높은 유병률과 재발률을 보이는 심각한 질병이다. 이에 따라 본 연구는 미래목표지향성과 실행의도에 기반한 음주조절훈련 프로그램을 개발해 그 효과를 검증하고자 하였다. 프로그램의 주 내용으로 전반부에 미래목표지향성을 높임으로써 행동 변화의 동기를 증진시키기 위한 ‘가능한 자기’ 훈련 등을 진행했고, 후반부에는 의도와 실제 행동 간의 간극을 좁히고 바람직한 행동 유지를 돕는 실행의도를 형성 및 훈련했다. 6회기로 구성된 음주조절훈련 프로그램을 병동에 입원 중인 알코올 사용장애 집단(18명)에게 주 2회 실시한 후 종속 측정치의 변화를 시작 시점에 1회, 프로그램 중간에 1회(3회기), 종료 시점에 1회 측정하여 기존의 병동 프로그램만 참여한 통제집단(10명)과 비교하였다. 집단(처치, 통제)과 시기(사전, 중간, 사후) 혼합설계에 따른 반복측정 변량분석(Repeated ANOVA) 검증을 실시하였고, 그 결과 사후시점에서 통제집단에 비해 처치집단이 유의미하게 증가된 금주 자기효능감, 미래목표지향성, 목표중심 자기조절 점수를 보였다. 즉, 본 프로그램에서 목표했던 미래지향적 동기와 행동 훈련에 대한 처치가 유의미했고, 결론적으로 금주 자기효능감이 증진되었음을 알 수 있었다. 이는 본 연구에서 개발한 프로그램이 현행되고 있는 알코올 치료 프로그램들의 한계를 보완하는 추가적인 프로그램으로서 유용할 수 있음을 시사한다.
끝으로 본 연구에 대한 임상적 함의와 제한점을 논의하였으며, 후속 연구에 대한 방향을 제안하였다.국문초록 ...ⅰ
목차 ...ⅱ
표 목차 ...ⅳ
그림 목차 ...ⅴ
Ⅰ. 서 론 ... 1
Ⅱ. 이론적 배경 ... 5
1. 알코올 사용장애 ... 5
1) 알코올 사용장애의 정의 ... 5
2) 알코올 사용장애로 인한 폐해 ... 6
3) 알코올 사용장애 치료 프로그램의 현황 ... 7
2. 금주 자기효능감 ... 8
3. 미래목표지향성과 금주 자기효능감 ... 9
4. 실행의도와 금주 자기효능감 ...12
Ⅲ. 연구목적과 연구문제 ... 15
Ⅳ. 미래목표지향성 및 실행의도에 기반한 음주조절훈련의 개발 및 효과 검증 ... 16
1. 프로그램 개발과정... 16
2. 미래목표지향성 및 실행의도에 기반한 음주조절훈련의 치료 요인 ... 17
1) 미래목표지향성 훈련 ... 17
2) 목표추구 행동의 실천 및 유지를 위한 실행의도 ... 18
3. 미래목표지향성 및 실행의도에 기반한 음주조절훈련의 주요 내용 ... 18
4. 방법 ... 19
1) 대상 및 절차 ... 19
2) 측정도구 ... 20
3) 분석방법 ... 22
Ⅴ. 결과 ... 22
1. 연구 참가자의 사전 동질성 ... 22
1) 인구통계학적 특성 및 관련 변인의 집단 간 사전 동질성 ... 22
2) 종속측정치의 집단 간 사전 동질성 ... 24
2. 미래목표지향성 및 실행의도에 기반한 음주조절훈련의 효과 ... 24
1) 종속측정치의 변화 ... 24
2) 금주 자기효능감에 미치는 효과 ... 26
3) 미래목표지향성에 미치는 효과 ... 28
4) 목표중심 자기조절에 미치는 효과 ... 30
Ⅵ. 논의 ... 32
1. 미래목표지향성 및 실행의도에 기반한 음주조절훈련의 효과 ... 32
2. 임상적 의의 ... 35
3. 한계 및 제언 ... 37
참고문헌 ... 38
부록 ... 49
ABSTRACT ... 82Maste
MIN-HO CHOI
No full text학위논문(석사)--아주대학교 일반대학원 :의생명과학과,2019. 2Ⅰ. 서론 1
Ⅱ. 실험재료 및 방법 3
1. 실험재료 3
2. PVA 나노섬유의 전기방사 및 제조방법 3
3. RGD-PVA 나노섬유의 전기방사 및 제조방법 4
4. 플루오레세인 나트륨 (Sodium fluorescein) 나노섬유의 제조방법 및 전기방사 4
5. MLE-12 세포 배양방법 4
6. 초대 간세포 (Primary hepatocytes) 분리방법 4
7. 초대 간세포 (Primary hepatocytes) 배양방법 5
8. 면역 형광 염색법 (Immunofluorescence) 5
9. 공초점 레이저 현미경 (confocal laser microscope) 5
10. 주사전자현미경 (scanning electron microscopy, SEM) 6
Ⅲ. 결과 7
1. 다양한 전기방사 조건 및 PAA와 GA 첨가에 따른 PVA 나노섬유 제작 7
2. RGD 펩타이드를 함유한 PVA 나노섬유 제작 10
3. RGD 펩타이드가 함유된 배양액이 MLE-12 세포 부착능에 미치는 영향 12
4. 농도별 RGD-PVA 나노섬유에서 MLE-12 세포의 부착능 실험 14
5. 플루오레세인 나트륨이 함유된 나노섬유 제작방법 및 방출되는 정도 16
6. PVA 나노섬유와 RGD 나노섬유에서 MLE-12 세포의 배양 및 형태 확인 18
7. PVA 나노섬유와 RGD-PVA 나노섬유에서 마우스 초대 간세포의 배양 및 형태 확인 20
8. PVA 나노섬유와 RGD-PVA 나노섬유에서 마우스 초대 간세포의 기능 테스트 23
Ⅳ. 고찰 25
Ⅴ. 결론 27
참고 문헌 28
영문 초록 31MasterRecently, the importance of nanofibers as a three-dimensional cell culture scaffold has been revealed. Research and development on nanofiber production using electrospinning has been actively conducted. Among various polymer polyvinyl alcohol (PVA), a water-soluble polyhydroxy polymer, is widely used due to its unique property of dissolving in a water, biocompatibility, biodegradability and permeability. However, PVA nanofiber is highly soluble in water which lead to low protein affinity and poor cell attachment. In this study, addition of PAA and GA to PVA solution resulted to the insolubility of the PVA nanofiber in water. RGD peptide, a major integrin binding domain, was mixed in PVA/PAA/GA solutions and fabricated using electrospinning. Cell adhesion and cell function were examined in RGD-PVA nanofiber membrane.. Surface morphology of nanofibers was analyzed by scanning electron microscope (SEM). MLE-12 cells and mouse hepatocytes were culture on PVA nanofibers and RGD-PVA nanofibers. The adherence, morphology, viability and proliferation of both cells were examined. As a result, cells cultured on RGD-PVA nanofibers showed better cell adhesion and growth stability then on PVA nanofibers alone. This study provides effective and simple way to confirm the possibility PVA nanofibers to be mixed with cell attaching peptides. Thus, RGD containing can be applied in three-dimensional cell culture
“Coastal Dynamics, Hazards, and Numerical Modelling” in Memory of Prof. Byung Ho Choi
No full textThis Special Issue is dedicated to Prof Byung Ho Choi at SungKyunKwan University, South Korea [...
Ion aggregation in high salt solutions. VI. Spectral graph analysis of chaotropic ion aggregates
No full textCarrying out molecular dynamics simulations and graph theoretical analyses of high salt solutions, and comparing numerically calculated vibrational spectroscopic properties of water with femtosecond IR pump-probe experimental data, we have recently found that ions in high salt solutions can form two morphologically different ion aggregate structures. In the cases of NaCl solutions, Na+ and Cl- tend to form compact cluster-like ion aggregate in high NaCl solutions. In contrast, K+ and SCN- form spatially extended network-like ion aggregates that also exhibit a percolating network behavior. Interestingly, a variety of graph theoretical properties of ion network in high KSCN solutions were found to be very similar to those of water H-bonding network. It was shown that spatially extended ion networks in high KSCN solutions are completely intertwined with water H-bonding networks, which might be the key to understand the high solubility of thiocyanate salts in water. Here, we further consider two salts that have been extensively studied experimentally by using femtosecond IR pump-probe technique, which are NaClO4 and NaBF4. Note that ClO 4 - and BF 4 - are well-known chaotropic ions that have been believed to behave as water structure breaker. To understand how such chaotropic ions affect water H-bonding structure, we carried out spectral graph analyses of molecular dynamics simulation data of these aqueous solutions. Graph spectra and degree distribution of ion aggregates formed in high NaBF4 and NaClO4 solutions show that these chaotropic anions also have a strong propensity to form ion networks. The fact that salts containing chaotropic ions like SCN-, BF 4 -, and ClO 4 - have very high solubility limits in water could then be related to our observation that these chaotropic anions with counter cations in high salt solutions are capable of forming intricate ion networks intertwined with water H-bonding networks. We anticipate that the present graph theoretical analysis method would be of use in further studying both various anomalous behaviors of interfacial water and fundamental physical chemistry of mixing and salt solubility in water. © 2016 Author(s)1771sciescopu
Compte rendu de : Le problème du temps chez Ferdinand de Saussure de Yong-Ho Choi
Get PDFeditorial reviewe
Polarized Raman study of large built-in strain in monolayer WS2 grown on Au/W substrate
No full text© 2022 Korean Physical Society. Strain plays a crucial role in the energy landscape of atomically thin two-dimensional materials. Here, we report polarized Raman results of WS2 monolayers having either rough or wrinkled surface morphologies. For rough WS2, the E′ phonon intensity exhibits polar behavior that deviates from the Raman polarization selection rules. Furthermore, with the formation of wrinkles on the WS2 surface, the E′ phonon splits into E′− and E′+ phonons. Polar plots of the E′− and E′+ phonon intensities as a function of polarization angle θ show that both phonons have strong polar dependence with orthogonal characteristics in their polarized intensity profiles: the E′− phonon intensity is maximum when the E′+ phonon intensity is diminished, and vice versa. Our result demonstrates that built-in strain in low-dimensional materials can be quantitatively identified by polarized Raman studies and further provides a useful strategy to achieve better device performance for which strain engineering is required.11Nsciescopuskc
Graph Theory and Ion and Molecular Aggregation in Aqueous Solutions
No full textIn molecular and cellular biology, dissolved ions and molecules have decisive
effects on chemical and biological reactions, conformational stabilities,
and functions of small to large biomolecules. Despite major efforts, the current
state of understanding of the effects of specific ions, osmolytes, and
bioprotecting sugars on the structure and dynamics of water H-bonding
networks and proteins is not yet satisfactory. Recently, to gain deeper insight
into this subject, we studied various aggregation processes of ions and
molecules in high-concentration salt, osmolyte, and sugar solutions with
time-resolved vibrational spectroscopy and molecular dynamics simulation
methods. It turns out that ions (or solute molecules) have a strong propensity
to self-assemble into large and polydisperse aggregates that affect both local
and long-range water H-bonding structures. In particular, we have shown
that graph-theoretical approaches can be used to elucidate morphological
characteristics of large aggregates in various aqueous salt, osmolyte, and
sugar solutions. When ion and molecular aggregates in such aqueous solutions
are treated as graphs, a variety of graph-theoretical properties, such as
graph spectrum, degree distribution, clustering coefficient, minimum path length, and graph entropy, can be directly calculated by considering an ensemble of configurations
taken from molecular dynamics trajectories. Here we show percolating behavior exhibited by ion
and molecular aggregates upon increase in solute concentration in high solute concentrations and
discuss compelling evidence of the isomorphic relation between percolation transitions of ion
and molecular aggregates and water H-bonding networks. We anticipate that the combination of
graph theory and molecular dynamics simulation methods will be of exceptional use in achieving
a deeper understanding of the fundamental physical chemistry of dissolution and in describing
the interplay between the self-aggregation of solute molecules and the structure and dynamics of
water
- …
