4,055 research outputs found
HO-1 is rapidly increased after haptoglobin and hemopexin infusion.
No full text(A and B) SS-mice (n = 3/group) were infused with vehicle or equimolar (1 μmol/kg) Hb, Hp, Hpx, Hb + Hp, or Hb + Hpx. Livers were removed and flash frozen 1 hour after infusion. Hepatic microsomes were used to assess heme oxygenase (HO) activity (A) via bilirubin production and protein expression (B) via immunoblot. Bars are means ± SD, **p (C and D) SS-mice (n = 3/group) were untreated or infused with Hp or Hpx (1 μmol/kg) at baseline (time 0). Livers were removed and flash frozen 24, 48 or 72 hours after infusion. Hepatic microsomes were used to assess (C) HO activity and (D) HO-1 protein expression via immunoblot. Bars are means ± SD, *p (E and F) SS-mice (n = 3/group) were infused with vehicle or increasing doses (0.0156, 0.0625, 0.25 or 1.0 μmols/kg) of Hp or Hpx at baseline. Livers and kidneys were removed and flash frozen 24 hours after infusion. Hepatic (E) and kidney (F) microsomes were used to assess HO activity. Bars are means ± SD.</p
The Impact of strategic implementation on the employees and contractors of the Hewlett-Packard Company.
No full textConducted within the global corporation of the Hewlett-Packard Company, (HP),this research examines employee and contractor responses to strategic implementation. The research environment is a relatively small UK group withinthe corporation that has continuously experienced significant change as new strategies were implemented. Employee and contractor responses to three separate cycles of change are analysed together with data drawn from the wider corporation by using a psychological contract framework, semi-structured interviewing, a research diary and secondary data from the literature and electronic sources. The research is essentially qualitative but draws on quantitative data where appropriate. A case study approach within an action research paradigm is the chosen methodology to allow consideration and triangulation of multiple sources of data relevant to the natural workplace setting. The research has confirmed a change from old or original psychological contracts to new contracts where employees and contractors are more insecure in, and more cynical of, their employment. This change is perceived as a
violation of their psychological contract and is causing employees and contractors to adopt a more mercenary approach to their employment with HP. The special bond between HP and its employees generated by the values and
egalitarian working environment created by its founders has been broken by new leadership. Acquisition of the Compaq Computer Company has further impacted employees and contractors resulting in declining morale and increasing scepticism about its proposed benefits. Indeed the very legitimacy of the acquisition and the leadership of HP are being questioned as HP changes to a new form. According to the founders, the values established that made HP successful in its first fifty years were expected to continue for at least another fifty years. This research shows how changing these values has collectively impacted
employees and contractors resulting in a major threat to the continuing existence of HP in its present form
High-pressure, High-temperature Synthesis, Magnetic Properties and X-ray Absorption Spectroscopy of Phases and hp- (RE=Tb, Ho, Er)
No full textInternational audienceRecent high-temperature high-pressure syntheses of binary phases RESn3-x (RE = Tb, Ho, Er) show good agreement with previously determined stability fields concerning pressure and temperature conditions. Phases hp-RESn3-x adopt a cubic Cu3Au arrangement (a = 466.52(6) pm for hp-TbSn3-x.; a = 465.44(8) pm for hp-HoSn3-x and a = 464.98(9) pm for hp-ErSn3-x). Superstructure reflections observed in experiments with synchrotron radiation indicate an ordered arrangement for HoSn3-x prepared at P = 8.4(8) GPa and T = 1270 K (space group Pm-3m). hp-HoSn3-x transforms exothermally at 460 K and ambient pressure into the low-pressure modification HoSn3-x indicating that the ordered Cu3Au-type arrangement is a metastable high-pressure phase. The hitherto unknown phases are identified as RE3Sn7 (RE = Ho, Er) being isotypic to the ambient pressure modifications Gd3Sn7 and Tb3Sn7 (space group Cmmm; lattice parameters a = 434.95(7) pm, b = 2615.6(6) pm, c = 442.09(7) pm for Ho3Sn7 and a = 434.29(5) pm, b = 2607.2(4) pm, c = 441.49(5) pm) for Er3Sn7. The crystal structure of RE3Sn7 is interpreted as an intergrowth of AlB2-, CaF2- and AuCu3-like segments. Analysis of the chemical bonding in Er3Sn7 by means of the electron localizability approach reveals two-centre Sn-Sn bonding in the AlB2-like segment and multi-centre bonding in the AuCu3- and CaF2-like motifs. The electrical resistivity of 31 µOhm cm at 300 K reveals that hp-HoSn3-x is a metallic conductor. The phases hp-RESn3-x and RE3Sn7 (RE = Ho, Er) show paramagnetic Curie-Weiss behaviour of the rare earth metal ions Ho3+ and Er3+, respectively. Ho3Sn7 and Er3Sn7 order antiferromagnetically at 10.0 K and 8.2 K, respectively. The high-pressure phases with 1 : 3 composition show no magnetic order above 2 K. XAS measurements on the compounds Tb3Sn7 and hp-TbSn3 indicate a 4f8 configuration evidencing the oxidation state +3 for terbium
ΔSRCR peripheral blood monocyte-derived macrophages (PMMs) still function as hemoglobin-haptoglobin (Hb-Hp) scavengers.
No full textA) Induction of HO-1 expression by Hb-Hp uptake. PMMs were incubated for 24 h in presence of 100 μg/ml Hb-Hp. RNA was isolated from cells and levels of heme oxygenase 1 (HO-1) mRNA determined by RT-qPCR (outlined bars uninduced, filled bars Hb-Hp uptake induced; red wild type, blue heterozygous, green ΔSRCR5). Expression levels were normalized using β-Actin expression levels and to the level of unstimulated HO-1 mRNA expression of each animal. Uninduced versus induced levels of HO-1 expression were analyzed by an unpaired t-test. Error bars represent SEM, n = 3. B) PMMs were incubated for 24 h in presence of 100 μg/mol Hb-Hp. PMMs were lysed with reducing SDS sample buffer and HO-1 protein expression analyzed by western blot. C) Quantification of Hb-Hp uptake. PMMs were incubated in presence of 10 μg/ml HbAF488-Hp for 30 min. Uptake of HbAF488-Hp was measured by FACS analysis (colored contour plots; red wild type, blue heterozygous, green ΔSRCR5) relative to isotype controls (grey). D) Visualization of Hb-Hp uptake. PMMs were incubated for 30 min with 10 μg/ml HbAF488-Hp. Cells were fixed, permeabilized and stained against CD163 and with DAPI. Representatively shown are pig 628 as wild type and 630 as ΔSRCR5 animals. Scale bar represents 40 μm.</p
Design, Deployment, and Evaluation of an Industrial AIoT System for Quality Control at HP Factories
No full textEnabled by the increasingly available embedded hardware accelerators, the capability of executing advanced machine learning models at the edge of the Internet of Things (IoT) triggers interest of applying Artificial Intelligence of Things (AIoT) systems for industrial applications. The in situ inference and decision made based on the sensor data allow the industrial system to address a variety of heterogeneous, local-area non-trivial problems in the last hop of the IoT networks. Such a scheme avoids the wireless bandwidth bottleneck and unreliability issues, as well as the cumbersome cloud. However, the literature still lacks presentations of industrial AIoT system developments that provide insights into the challenges and offer lessons for the relevant research and industry communities. In light of this, we present the design, deployment, and evaluation of an industrial AIoT system for improving the quality control of HP Inc.’s ink cartridge manufacturing lines. While our development has obtained promising results, we also discuss the lessons learned from the whole course of the work, which could be useful to the developments of other industrial AIoT systems for quality control in manufacturing.Nanyang Technological UniversitySubmitted/Accepted versionThis study is supported under the RIE2020 Industry Alignment Fund-Industry Collaboration Projects (IAF-ICP) Funding Initiative, as well as cash and in-kind contribution from the industry partner, HP Inc., through the HP-NTU Digital Manufacturing Corporate Lab
Persistent HP-specific Th17 responses in individuals with past HP infection.
No full text<p>(A) The IL-17A response of PBMCs to HP lysate. 2×10<sup>6</sup> PBMCs from the three groups of patients in this study were incubated for 72 hours in a 96-well plate, together with 10 µg/ml whole bacterial lysate prepared from HP strain 26695. Subsequently, culture supernatant was harvested and assayed for IL-17A production by ELISA. The fold increase in IL-17A production is depicted, i.e. (cytokine concentration in wells containing PBMCs stimulated with HP lysate) ÷ (cytokine concentration in control wells containing unstimulated PBMCs). Group A (n = 7), group P (n = 17), and group N (n = 10). NS: not significant, *p<0.05. (B) The HP-specific IL-17A response of CD4<sup>+</sup> T cells purified from PBMCs. PBMCs were pulsed with HP strain 26695 lysate for 24 hours, and then irradiated at 40 Gy. These cells were used as APCs, and co-cultured for 48 hours with purified autologous CD4<sup>+</sup> T cells at a ratio of 5 APC: 1 CD4<sup>+</sup> T cell. In wells where MHC class II blocking antibody (10 µg/ml) was used, antibody was incubated with APCs for 1 hour prior to addition of responder CD4<sup>+</sup> T cells. In control wells, APCs were incubated with an equal concentration of mouse IgG2a isotype control antibody for the same length of time. IL-17A levels in culture supernatant were measured using ELISA. The response from one representative group P patient has been depicted. (C) The HP-specific IL-17A response of gastric mucosal LPMCs. HP-pulsed PBMCs were prepared as in (B) for use as APCs. Autologous LPMCs expanded from gastric biopsy samples were used as responders, and IL-17A levels in culture supernatant were measured by ELISA after 48 hours of co-culture. The response from one representative group P patient has been depicted.</p
Evolution of the use, effectiveness and safety of bismuth-containing quadruple therapy for Helicobacter pylori infection between 2013 and 2021 : results from the European registry on H. pylori management (Hp-EuReg)
No full textPublisher Copyright: © Author(s) (or their employer(s)) 2025.background Bismuth quadruple therapies (BQTs) including bismuth, a proton pump inhibitor (PPI) and two antibiotics have been shown to be highly effective for treating Helicobacter pylori infection even in areas of high bacterial antibiotic resistance. Objective To describe the time trends of use, effectiveness and safety of BQT in Europe using the European Registry on Helicobacter pylori Management (Hp-EuReg). Design Patients registered in the Hp-EuReg from 2013 to 2021 who had received BQT were included. The regimens prescribed, the number of eradication attempts, effectiveness, adherence and safety were analysed. The effectiveness was assessed by modified intention to treat (mITT). Time-trend and multivariate analyses were performed to determine variables that predicted treatment success. results Of the 49 690 patients included in the Hp-EuReg, 15 582 (31%) had received BQT. BQT use increased from 8.6% of all treatments in 2013 to 39% in 2021. Single-capsule BQT—containing bismuth, metronidazole and tetracycline—plus a PPI (single-capsule BQT, ScBQT) was the most frequent treatment mode (43%). Schemes that obtained an effectiveness above 90% were the 10-day ScBQT and 14-day BQT using tetracycline plus metronidazole, or amoxicillin plus either clarithromycin or metronidazole. Only ScBQT achieved above 90% cure rates in all the geographical areas studied. Using the ScBQT scheme, adherence, the use of standard or high-dose PPIs, 14-day prescriptions and the use of BQT as first-line treatment were significantly associated with higher mITT effectiveness. Conclusion The use of BQT increased notably in Europe over the study period. A 10-day ScBQT was the scheme that most consistently achieved optimal effectiveness.Peer reviewe
Direct numerical simulation of the turbulent flow around an airfoil using spectral/HP method
No full textSince the accuracy of the widely used Reynolds Averaged Navier Stokes (RANS) approach is limited simulating turbulent flow separation on airfoils, we applied a spectral/hp element method to predict the turbulent flow separation at an airfoil for an angle of attack of 12°. The spectral/hp approach allows to solve the incompressible Navier-Stokes equation with spectral accuracy on unstructured grids by means of Direct Numerical simulation (DNS). Here we present first results we obtained from DNS of the flow around a fx79-w151a airfoil section for a low Reynolds Number = 5000
Omzetting van HP-plotter commando's naar EF 9365 commando's: Taakverslag
No full textHet reeds bestaande programma TVPLOT verzorgt de omzetting van commando's voor de HP 7221 C kleurenplotter naar commando's voor de kleurenmonitor. Het tekenen gebeurt via het programma PLOT. Dit programma PLOT is nu vervangen door de Grafische Display Processor EF 9365 die meer mogelijkheden biedt Om deze vervanging mogelijk te maken zijn in het programma TVPLOT de noodzakelijke wijzigingen aangebracht Dit programma en de wijzigingen zijn geschreven in assemblertaal voor de M6800 microprocessor.Applied SciencesElectrotechniekAutomatische Verkeerssysteme
Functional characterization of haptoglobin (Hp) in breast cancer
No full textBackground: Altered energy metabolism is one of the hallmarks in cancer which is characterized by preferential dependence on glycolysis. HP is a secreted glycoprotein which binds to free hemoglobin and prevent the loss of iron. Elevated HP was detected in patients with inflammatory diseases and a variety of cancers. In this study, we aim to investigate the functional roles of HP in breast cancer especially the relationship with glycolysis pathway.
Methods: MTT, cell cycle, apoptosis, aldehyde dehydrogenase (ALDH) activity and glucose uptake assay were performed in triple negative breast cancer cells (MDA-MB-231 and MDAMB-468). Gene expression was analyzed by qRT-PCR and serum HP concentration was evaluated by enzyme-linked immunosorbent assay (ELISA). Lactate dehydrogenase (LDH) activity kit was used for enzyme activity quantification.
Result: HP was overexpressed in cancer tissues and serum of breast cancer patients. Knockdown
by siRNA led to decreased cell proliferation which was associated with G1 phase cell cycle arrest and increased early apoptotic cells. ALDH activity was inhibited upon HP knockdown. Glucose uptake and LDH activity was significantly impeded in breast cancer cells and cell culture supernatant, respectively. Also, qRT-PCR analysis revealed that glycolysis-related genes were altered upon HP silencing.
Conclusions: Our data suggested that HP is overexpressed in breast cancer with oncogenic role and it is involved in glycolysis. These findings may provide novel diagnostic and therapeutic strategies towards this malignant disease
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