153 research outputs found
PIST (GOPC) modulates the oncogenic voltage-gated potassium channel KV10.1
No full textAlthough crucial for their correct function, the mechanisms controlling surface expression of ion channels are poorly understood. In the case of the voltage-gated potassium channel KV10.1,this is determinant not only for its physiological function in brain, but also for its pathophysiology in tumors and possible use as a therapeutic target. The Golgi resident protein PIST binds several membrane proteins, thereby modulating their expression. Here we describe a PDZ domain-mediated interaction of KV10.1and PIST, which enhances surface levels ofKV10.1. The functional, but not the physical interaction of both proteins is dependent on the coiled-coil and PDZ domains of PIST; insertion of eight amino acids in the coiled-coil domain to render the neural form of PIST (nPIST) and the corresponding short isoform in an as-of-yet unknown form abolishes the effect. In addition, two new isoforms of PIST (sPIST and nsPIST) lacking nearly the complete PDZ domain were cloned and shown to be ubiquitously expressed. PIST and KV10.1 co-precipitate from native and expression systems. nPISTalso showed interaction, but did not alter the functional expression of the channel. We could not document physical interaction betweenKV10.1 and sPIST, but it reduced KV10.1 functional expression in a dominant-negative manner. nsPIST showed weak physical interaction and no functional effect on KV10.1. We propose these isoforms to work as modulators of PIST function via regulating the binding on interaction partners
”Babes in the wood?”: Intertekstuaalisuus ja subteksti Solveig von Schoultzin novellissa ”Även dina kameler”
No full text”Babes in the wood?” – Intertextuality and subtext in the short story ”Även dina kameler” by Solveig von Schoultz
In my article I examine three central intertexts in the short story “Även dina kameler” (Even your camels) written by the Finland-Swedish author Solveig von Schoultz in 1965. The short story includes several, “odd” intertextual fragments, which all seem to point at a secret of some kind, hidden from the reader. In my analysis I use the definition of the term “subtext”, put forward by the literary critic Michael Riffaterre in his book Fictional Truth, in order to show how the mysteriousness of the text is constructed, how the intertexts build up the “subtext” of the short story and what the secret is that the story both hides and signals of. This way one gets a picture of how the seemingly plain and realistic text is actually built up in an effective and elaborate way and characterised by high textual density.
The analyzed intertexts all relate to certain topics: a mother, who is distant or dead, a woman’s identity and changes in it, and how words get or loose their meaning. All in all, the short story shows both on its explicit and hidden level how the death of the protagonist’s mother – the hidden secret of the text – has lead to the creation of a language of one’s own. The significance of language is, then, connected to loss. The strange words and allusions the woman protagonist uses also put forward the metalinguistic and poetic message of the story; the importance of language, and how language can both carry meanings, and become empty of meaning. In this way the story is even connected to the author’s own enterprise
Aesops Fabler
No full textThis slim book of some 24 pages was apparently a Christmas gift of the Bergen Faktorforening. In fact, Wiig had done a 1951 translation of Aesop 106 pages in length, illustrated by Johan Berle Reidar and published by J.W. Eides. I have found a copy available and have ordered it. I am delighted to find this book from Bergen and from a Bergen bookseller because I did not have time to seek out booksellers during our short stay there this summer. Strong endpapers offer a forest with a lion attacking, a squirrel (?) fleeing, and a donkey prancing. The Aesop of the cover and title page look to me as though they were in India. That cover has a background of green and gold for its line-drawing of the seated fabulist. In the book's first illustration, miller and son both are bent in dejection. I need a good Norwegian to tell me what happened to their ass! There are two strong, highly interpretative illustrations for OR and three for FG. Similarly, the artist takes two moments to picture in AD. The second pictures quite dramatically and minutely the ant biting the big toe of the hairy hunter. Is that Androcles in the last picture? Is there a text to accompany this picture? It is a feather in the cap of this collection that a rare book like this becomes available here. Online I could find only four copies. Two are in Denmark, one is in China, and the last is at the University of Southern California. This copy has a slightly musty smell.This is a hardbound book (hard cover)Language note: NorwegianLimited to 300 copiesOversatt av Hanna Wii
Solveig to Dear friend - James Meredith (Undated)
No full textSigned by Solveighttps://egrove.olemiss.edu/mercorr_pro/1956/thumbnail.jp
Literacy Events in Writing Play Workshops with Children Aged Three to Five: A Study of Agential Cuts with the Artographic Triple Dimensions as a Lens
No full textThe aim of this article is to explore how the multiple perspectives offered by an artographer’s lens contribute to three literacy events generated by writing play activities for children three to five years old. These events are part of a more comprehensive study of emergent literacy in writing play workshops, focusing on writing in different displays and with different writing tools. The artographer in the comprehensive study is Solveig Åsgard Bendiksen, also the first author in this article. The two other co-authors contribute with artographic methodology and with concepts from agential realism in the analysis of three literacy events. The intra-actions between the artographer, the children, the affects, the affordance of rich materials, and the context as performative agents in diffractive reading produced a number of findings concerning emergent writing literacy, especially concerning emergent cultural literacy
Functional characterization of genes involved in the regulation of EMT and somitogenesis in the mouse embryo
No full textDie Embryogenese beruht auf einer Vielzahl von räumlich und zeitlich
kontrollierten Prozessen, die aufeinander aufbauen. Ein wesentlicher Prozess
bei der Bildung der Körperanlage und bei der Organogenese ist die
Mesodermbildung. Sie beginnt mit der epithelialen mesenchymalen Transition
(EMT), bei der epitheliale Zellen in mesenchymale Zellen umgestaltet werden,
die dann in verschiedene Richtungen weiter differenzieren können. Darunter
befindet sich das sogenannte präsomitische Mesoderm (PSM), aus dem die Somiten
hervorgehen. Somiten sind u.a. die Vorläufer der Wirbelkörper und der
Skelettmuskulatur. Embryonale Prozesse wie die Mesodermbildung werden durch
noch nicht vollkommen aufgeklärte Netzwerke von Regulatorgenen gesteuert. Eine
Charakterisierung des Zusammenspiels aller beteiligten Regulatorgene ist daher
elementar für die Aufklärung von Vorgängen in der frühen Embryogenese, wie EMT
und Mesodermbildung und würde zudem zum Verständnis von Vorgängen in der
Tumorprogression und Metastasierung beitragen, da die EMT auch hierbei einen
zentralen Vorgang darstellt. P19 Embryonale Carcinoma (P19 EC) Zellen dienten
als Zellkulturmodell pluripotenter Stammzellen in vitro. Sie können in vitro
zur Mesodermbildung induziert werden und damit zur Identifizierung von
Regulatorgenen der Mesodermbildung verwendet werden. Die Charakterisierung der
P19 EC Zellen mittels einer Antikörpermarkierung wies auf einen epithelialen
Zelltyp mit Stammzelleigenschaften hin. Sie exprimierten die Markergene Cdh1
und Oct4, die epitheliale Zellcharakteristik und Pluripotenz anzeigen; eine
Expression des mesenchymalen Markers Vim wurde im Gegensatz dazu nicht
detektiert. Mittels Aktivierung der Wnt-Signalkaskade, die ein bei der EMT
wichtiges Regelnetzwerk darstellt, sollte eine gezielte Induktion der P19 EC
Zellen in Richtung Mesoderm ermöglicht werden. Alternativ sollte eine
Differenzierung der P19 EC Zellen zu Mesoderm bzw. PSM mittels der Zugabe von
DMSO oder einer Tbx6-Überexpression in den P19 EC Zellen untersucht werden.
Mithilfe einer Microarray-Analyse sollte ein Gesamtüberblick über die sich
dabei in ihrem Expressionsniveau verändernden Gene möglich werden. Durch
Funktionsanalysen einzelner sich darin signifikant verändernder Gene wäre eine
Eingliederung dieser Gene mit ihrer Funktion bei EMT und Somitogenese sowie im
Embryo möglich. Eine gezielte Aktivierung der Wnt-Signalkaskade durch die
Induktion mit Wnt3a oder SB216763, als Inhibitor von Gsk3β, einem wichtigen
Enzym in der Wnt-Signalkaskade, war nicht möglich. P19 EC Zellen ließen sich
demnach nicht mittels der Wnt-Signalkaskade zu einer Differenzierung en bloc
in Richtung Rumpfmesoderm induzieren. Eine unspezifische Mesoderm-Induktion
der P19 EC Zellen mit DMSO war dagegen möglich. Mittels
Expressionsprofilierung auf einem Microarray konnten Mesoderm-spezifische Gene
identifiziert werden. Der gleiche Effekt konnte allein durch Überexpression
von Tbx6, einem essentiellen Regulator im PSM, erzielt werden. 24 Gene wurden
bereits durch die alleinige Tbx6-Überexpression in den P19 EC Zellen
beeinflusst. Aus diesen Genen wurden 12 auch durch die DMSO-Induktion allein
reguliert. Die Überexpression des Transkriptionsfaktors Tbx6 in Kombination
mit DMSO-Induktion begünstigte die Differenzierung der P19 EC Zellen in
Richtung PSM. Tbx6 führte unter anderem zur Aktivierung der Regulatorgene
Mixl1, Nrarp und Tnfrsf19, die für die weitere Funktionsanalyse mittels RNA-
Interferenz ausgewählt wurden. Ein „knockdown“ der Expression in P19 EC- und
in ES Zellen war aber mit den dafür hergestellten Konstrukten nur mit Tnfrsf19
erzielbar. In vivo im Embryo erzielte jedoch auch dieses knockdown-Konstrukt
keinen Phänotyp. Weitere Funktionsanalysen mittels Überexpression von Tnfrsf19
und einer vermutlichen dominant negativen Variante dieses Gens in vivo zeigten
ebenfalls keinen Phänotyp. Tnfrsf19 wurde sowohl als Zielgen des Wnt-
Signalwegs als auch, in den eigenen Analysen aufgrund der Microarray-Daten,
als Zielgen von Tbx6 identifiziert und scheint somit eine Rolle bei der
Bildung von PSM zu spielen. Die vorliegende Arbeit erbrachte einen Hinweis auf
eine Rolle von Tnfrsf19 bei der Bildung von PSM sowie der Somitogenese und
identifizierte weitere Kontrollgene der EMT und Mesodermbildung im Mausembryo.Embryogenesis is based on many locally and temporally controlled processes
which are coordinated to each other. The formation of mesoderm is an essential
process while forming the anlage of the bodyplan and during organogenesis. It
starts with the epithelial mesenchymal transition (EMT) in which epithelial
cells are transfigured to mesenchymal cells which are, then, able to
differentiate into distinct directions. Among these mesenchymal cells the so
called presomitic mesoderm (PSM) is situated, from which the somites emerge.
Somites are the precursors of the vertebrae and the skeletal muscles.
Embryonic processes such as mesoderm formation are controlled by signalling
cascades of regulatory genes, which are yet not fully analyzed. The
characterization of the interaction of all regulatory genes is therefore
fundamental for the understanding of processes of early embryogenesis such as
EMT and mesoderm formation. It also would help to understand processes during
tumor progression and metastasis as EMT is also a pivotal event during these
processes. P19 Embryonic Carcinoma (P19 EC) cells served as a cell culture
model for pluripotent stem cells in vitro. They can be induced to formate
mesoderm in vitro. They are, therefore, predestined to identify regulatory
genes in mesoderm formation. The characterization of P19 EC cells using
antibody staining furthermore indicates an epithelial cell type with stem cell
features. The cells expressed the genes Cdh1 and Oct4 which are essential to
identify epithelial cell characteristics and pluripotency. In contrast the
cells didn`t express Vim, which is an essential gene to identify mesenchymal
characteristics. Directed induction of P19 EC cells to differentiate to
mesoderm should be possible by activation of the Wnt signalling cascade, which
is an essential regulatory network of EMT. Alternatively differentiation of
P19 EC cells to mesoderm or presomitic mesoderm should be analyzed while using
DMSO or overexpression of Tbx6 in P19 EC cells. An overview of all genes
changing their expression level should be analyzed via expression profiling on
microarrays. Functional characterization of individual genes, which are
significantly modified in their expression level, could help to integrate
these genes and their function in EMT, somitogenesis as well as in the embryo.
It was not impossible to induce directly the Wnt signalling cascade in P19 EC
cells using Wnt3a or SB216763, an inhibitor of Gsk3β, an important enzyme of
the Wnt signalling cascade. According to that there was no measured induction
of the Wnt signalling cascade in P19 EC cells to differentiate en bloc into
trunk mesoderm. It was possible to induce a non-specific mesoderm induction of
P19 EC cells with DMSO. Mesoderm-specific genes were identified via expression
profiling on microarrays. The same effect could be attained with exclusive
overexpression of Tbx6, an essential regulatory gene within the PSM. 24 genes
were influenced by the exclusive Tbx6-overexpression in P19 EC cells. 12 of
those 24 genes were also regulated exclusively by DMSO-induction.
Overexpressing the transcription factor Tbx6 in combination with DMSO was
promoting the differentiation of P19 EC cells towards PSM. Among other things
Tbx6 were activating the regulatory genes Mixl1, Nrarp and Tnfrsf19, whose
functions were to be analyzed via RNA-interference. The only significant
“knockdown” of the expression in P19 EC and ES cells with the established
constructs was attained with Tnfrsf19. However, in vivo no embryonic phenotype
was observed using this construct. Further functional analysis of
overexpressing Tnfrsf19 and a probably dominant-negative isoform of this gene
did likewise not show any phenotype. Tnfrsf19 as target gene of the Wnt-
signaling cascade was also identified as target gene of Tbx6 in our analysis
of the microarray data. Thus it seems to be relevant in the formation of PSM.
The present thesis supplied indication on Tnfrsf19 being relevant in the
formation of PSM and in somitogenesis and identified controlling genes of EMT
and mesoderm formation in the mouse embryo
Risk factors for lameness in cubicle housed Austrian Simmental dairy cows
No full textAustrian dairy farming is characterised by predominant use of Simmental cows on small-scale farms. Our aim was to identify lameness risk factors related to housing and management in cubicle housed Austrian dairy cows. Furthermore, we used animal-based parameters (ABP) as integrated measures of cubicle quality and feeding management. The first author visited 30 farms in eastern Austria with 24-54 cows (mean = 35) in the milking herd during winter housing period, and collected data on housing, management, behaviour, and lameness via direct observations and an interview (part of Welfare Quality(R) project). Mean lameness prevalence was 31% (range 6-70%). Data were analysed using logistic regression with generalised estimating equations (GEE). The final model was based on 832 cows and included six risk variables, five ABP, and the significant confounders 'county' and 'lactation number'. Odds for lameness increased with decreasing lying comfort, except for cubicle width. The following lying-related factors were significant in the final model (odds ratios (OR) in brackets): mats/mattresses as opposed to deep bedded cubicle base (1.61), length of lying area (OR 186-191 vs. 3.5 had at least 0.39 lower odds of being lame, while cows with suboptimal milk protein content (3.8%) had 1.37 times higher odds. Odds for lameness clearly increased with age (OR lactation >4 vs. 1 = 3.38). In sum, lying comfort and nutrition are key areas for lameness prevention on modern dairy farms in Austria with herd sizes above 30 cows. (C) 2009 Elsevier B.V. All rights reserved.European Commission [FOOD-CT-2004-506508
One-year trial of 12-hour shifts in a non-intensive care unit and an intensive care unit in a public hospital: a qualitative study of 24 nurses’ experiences
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Qualitative research
Research
One-year trial of 12-hour shifts in a non-intensive care unit and an intensive care unit in a public hospital: a qualitative study of 24 nurses’ experiences
Solveig Osborg Ose, Maria Suong Tjønnås, Silje Lill Kaspersen, Hilde Færevik
Author affiliations
Abstract
Objectives The aim of this study was to provide recommendations to hospital owners and employee unions about developing efficient, sustainable and safe work-hour agreements. Employees at two clinics of a hospital, one a non-intensive care and the other a newborn intensive care unit (ICU), trialled 12-hour shifts on weekends for 1 year.
Methods We systematically recorded the experiences of 24 nurses’ working 12-hour shifts, 16 in the medical unit and 8 in the ICU for 1 year. All were interviewed before, during and at the end of the trial period. The interview material was recorded, transcribed to text and coded systematically.
Results The experiences of working 12-hour shifts differed considerably between participants, especially those in the ICU. Their individual experiences differed in terms of health consequences, effects on their family, appreciation of extra weekends off, perceived effects on patients and perceived work task flexibility.
Conclusions The results indicate that individual preference for working 12-hour shifts is a function of own health situation, family situation, work load tolerance, degree of sleep problems, personality and other factors. If the goal is to recruit and retain nurses, nurses should be free to choose to work 12-hour shifts
Hazard characterization of Alternaria toxins to identify data gaps and improve risk assessment for human health (Archives of Toxicology, (2024), 98, 2, (425-469), 10.1007/s00204-023-03636-8)
No full textPublisher Copyright: © The Author(s) 2024.In this article the affiliation details for Authors Anne Straumfors and Solveig Krapf were incorrectly given as ‘Norwegian Veterinary Institute, PO Box 64, 1431 Ås, Norway’ but should have been ‘National Institute of Occupational Health, Gydas Vei 8, 0363, Oslo, Norway’. Furthermore, the affiliation details for Authors Eszter Borsos and Francesco Crudo were incorrectly given as “Food Hygiene and Technology, University of Veterinary Medicine, Vienna, Veterinärplatz 1, 1210, Vienna, Austria”, but should have been “Department of Food Chemistry and Toxicology, Faculty of Chemistry, University of Vienna, Austria”.publishersversionpublishe
Lennart Nilsson's Fish-Eyes : A Photographic and Cultural History of Views from Below
No full textThis article examines the Swedish photo-grapher Lennart Nilsson’s wide-angle imagery in its production and circulation contexts from the mid-1960s to the 1980s. It focuses in particular on a sample of photographs, labelled “fish-eye” because of the specific nature of their distorted perspective, which he produced for magazines, books and films during this period. The aim is to shed light on how these pictures contributed to stimulating an alternative mode of spectatorship, the view from below, challenging the viewing position created by the traditional linear perspective of painting and photography. The author shows how an immersive and transparent experience of the images could exist side by side with its apparent opposite: the hypermediated experience of the same type of image. It is also suggested that Nilsson’s fish- eye photographs have been important in naturalizing a specific way of seeing foetal life in contemporary visual and media culture
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