27 research outputs found
sj-docx-1-eso-10.1177_23969873231223062 – Supplemental material for Certification of stroke centers at primary hospitals and the improvement of thrombolysis n South China during 2020–2022
No full textSupplemental material, sj-docx-1-eso-10.1177_23969873231223062 for Certification of stroke centers at primary hospitals and the improvement of thrombolysis n South China during 2020–2022 by Yicong Chen, Qingfeng Lei, Jiaoxing Li, Jianle Li, Yinxin He, Jingjing Li, Shihui Xing, Yuhua Fan and Jinsheng Zeng in European Stroke Journal</p
[[alternative]]A Study on the Buddhism Thought of Wang, Xiangliu
No full text[[abstract]] 本論文旨在研究印心宗第二代傳人王驤陸(相六)居士的佛學思想，以及探討其經典詮釋之特色。研究方法採用傅偉勳先生的「創造的詮釋學」研究法。論述了王驤陸之生平學術發展，及其與印心宗之關係。王驤陸居士的佛學思想，重圓融、重次第、重修證，解經不依文解義，並以此評論佛教各宗，如淨土宗、禪宗、密宗。王驤陸居士在弘法時注解了許多經典，唯部分著作於文革時散佚。其注經重視分品，頗有其特色。[[abstract]] The aim of this article is to investigate the Buddhist ideology of Householder Wang﹐Xiangliu, the second patriarch of the Yinxin sect of Chinese Esoteric Buddhism, and explore his interpretations of the characteristics of the Classics. This study uses professor Fu﹐ Weihsun's ＂Creative Hermeneutics＂ for research. Discussion is on Wang﹐Xiangliu's lifelong academic development and its relationship to the Yinxin sect. Householder Wang﹐ Xiangliu's Buddhist ideology attaches great importance to harmony, order, and correction, and a flexible interpretation of holy texts, in writing his commentaries on Buddhist sects such as Pure Land Buddhism, Zen Buddhism and Esoteric Buddhism. In his preaching, he explains many of the the Classics, though many of his writings were lost in the Cultural Revolution. A special feature of his teachings on the holy texts is the emphasis on grading and accommodating practitioners of all ability and aptitude
Isolation and characterization of a marine bacterium producing protease from Chukchi Sea, Arctic
No full textA gram negative bacterium Ar/W/b/75°25'N/1 producing extracellular alkaline protease was isolated from surface water of latitude 75°25'N, and longitude 162°25'W in Chukchi Sea, Arctic. The strain can grow at the temperature range from 7°C to 30°C, and grow better at 30°C. It can not grow at 40°C. Keeping certain salinity concentration in medium is necessary for cell growth. It grows well in medium containing salinity concentration from 0.5% to 10% sodium chloride. Glucose, sucrose and soluble starch can be utilized by the strain, among which glucose is the optimal carbon source. Peptone is the optimal organic nitrogen source for cell growth and protease producing, and ammonium nitrate is the optimal inorganic nitrogen source. About 75.7% of total protease of the strain are extracellular enzyme. Optimal temperature for proteolytic activity is at 40°C. Protease of the strain keeps stable below 40°C, and shows high proteolytic activity within the pH range from 7 - 11
Role of lethal giant larvae homolog 1 gene in drug resistance of pancreatic cancer cells.
No full text背景和目的：胰腺導管腺癌（簡稱胰腺癌）是世界範圍內惡性程度最高的癌癥之一，目前它的5 年生存率不到5%。大部分的病人在診斷初期就已經發展到了局部浸潤或遠處轉移的階段，因此失去了根治性手術切除的机会。輔助性化療對於胰腺癌病人來說是一個首選的治療方案，但是目前只有一小部分病人對化療藥物有良好的反應，而臨床化療失敗常與腫瘤細胞對化療藥物產生耐藥有關。吉西他濱是目前臨床上常用的一線抗癌藥物，但是它的耐藥現象在胰腺癌病人中廣泛存在，也是阻礙其臨床應用的主要原因之一。盡管已經有很多研究致力於揭示吉西他濱在胰腺癌細胞中的耐藥機理，目前臨床上仍然沒有有效的方法應對吉西他濱耐藥。我們的研究主要是為了探討一些以前沒有报道過的參與吉西他濱耐藥機理的基因，借此揭示胰腺癌細胞的吉西他濱耐藥的深層機制，為臨床上的治療提供理論依據。實驗方法：我們實驗室之前在胰腺癌細胞株Capan2 中用全基因組RNAi篩選的方法確定LLGL1 作為抑癌基因能增強吉西他濱在胰腺癌細胞中的細胞毒性。我們隨後用體外細胞毒性分析實驗和皮下腫瘤動物模型來驗證LLGL1 是否能增強吉西他濱的細胞毒性，用蘇木素-伊紅染色和原味末端轉移酶標記技術分析抑制LLGL1 的表達是否會影響吉西他濱誘導的細胞雕亡反應。我們還應用微陣列分析技術進一步探尋LLGL1 的下遊靶蛋白，用實時定量PCR(qRT-PCR) 、蛋白印跡法（western blotting）、熒光素酶檢測等技術來進一步證實LLGL1 與下遊靶蛋白的關系，用免疫組織化學方法探究LLGL1 下遊靶蛋白在胰腺癌組織中的表達情況，以及該蛋白與LLGL1 的表達相關性，還應用染色體免疫共沈澱的方法探討轉錄因子Sp1(pThr453) 和RNA 聚合酶 II 在LLGL1 下遊靶蛋白的啟動子上的富集情況。實驗結果：LLGL1 能增強吉西他濱在胰腺癌中的細胞毒性，抑制該基因的表達能誘導胰腺癌細胞對吉西他濱的耐藥，而上調該基因的表達則會增強胰腺癌細胞對吉西他濱的細胞毒性反應。OSMR 是LLGL1 的下遊靶蛋白, 其在胰腺癌組織中的表達與LLGL1 呈負性相關，抑制OSMR 的表達可以逆轉由LLGL1表達下調引起的吉西他濱耐藥現象。OSMR 表達上調可以增強腫瘤幹細胞標記物CD44 和CD24 的表達。另外，在胰腺癌細胞中，抑制LLGL1 的表達能激活ERK2/Sp1 信號通路，導致磷酸化Sp1(pThr453)的表達升高。OSMR 啟動子既沒有TATA 元件也沒有INR 元件，但是有Sp1 结合元件可供Sp1 結合。磷酸化Sp1(pThr453)可以結合到OSMR 啟動子的Sp1 结合元件上，從而促使RNA 轉錄酶II 結合到該啟動子上，啟動OSMR 基因的轉錄。結論：我們的研究發現：1，LLGL1 能增強吉西他濱在胰腺癌中的細胞毒性，抑制該基因在胰腺癌細胞中的表達能上調OSMR 的表達，並誘導吉西他濱耐藥；2，OSMR 的表達在胰腺癌組織中與LLGL1 呈負性相關；3，下調LLGL1的表達能激活ERK2/Sp1 信號通路，進一步導致磷酸化Sp1(pThr453)和RNA 轉錄酶II 在OSMR 啟動子上的聚集，最終促使OSMR 的高表達，而下調LLGL1的表達能抑制該調節通路，從而抑制OSMR 的轉錄。Background & Aims: Pancreatic ductal adenocarcinoma (PDAC) is one of the most malignant cancers worldwide. Its 5-year survival rate is less than 5%, because most patients have already developed to the advanced stage of local invasion or distant metastasis once diagnosed, and missed the chances of curable surgical resection. Adjuvant chemotherapy is an alternative therapeutic strategy against PDAC. Yet, only very small proportion of patients could benefit from chemotherapy due to the innate and easily-acquired chemo-resistance in PDAC cells, especially to the first-line chemotherapeutic drug, gemcitabine. Many studies have been conducted to exploring the mechanisms underlying gemcitabine resistance in PDAC cells, but gemcitabine resistance is still the major obstacle impeding PDAC patients benefits from chemotherapy. Our studies aimed to investigate novel genes involved in gemcitabine response and to explore the undefined mechanisms generating gemcitabine resistance in PDAC cells.Methods: Our colleagues previously performed genome-wide RNAi screening in gemcitabine-sensitive Capan2 cells. Lethal giant larvae homolog 1 (LLGL1) was identified as a potential gemcitabine-sensitizing gene which was then validated by our subsequent in-vitro drug cytotoxicity assay in LLGL1-inhibited Capan2 and SW1990 cells and in vivo subcutaneous xenograft mouse model. Hematoxylin & Eosin staining and terminal Deoxynucleotidyl Transferase dUTP Nick End Labeling were applied for the assessment of apoptotic effects induced by gemcitabine in subcutaneous xenografts. We did gene expression microarray analysis to explore the potential downstream targets of LLGL1. Western blotting, qRT-PCR, and luciferase assay were applied to validate the downstream target of LLGL1 that were figured out by microarray analysis. We also did immunohistochemical staining to investigate the expression levels and correlationship of LLGL1 and its downstream target in PDAC specimens. Chromatin immunoprecipitation was performed to explore the enrichment of the transcriptional factor Sp1(pThr453) and RNA polymerase II (Pol II) at the promoter of the downstream targets of LLGL1.Results: LLGL1 was identified as a gemcitabine-sensitizing gene, whose inhibition remarkably reduced gemcitabine response in gemcitabine-sensitive Capan2 and SW1990 cells, and ectopic expression induced gemcitabine response in gemcitabine-resistant PANC1 cells. Oncostatin M receptor (OSMR) was identified as a downstream target of LLGL1, whose expression was negatively correlated with LLGL1, and knockdown of OSMR significantly reversed gemcitabine resistance induced by LLGL1 inhibition in Capan2 and SW1990 cells. Additionally, activation of OSMR signaling was associated with the elevated expression of cancer stem cell markers, CD44 and CD24, both of which had already been identified to contribute to gemcitabine resistance in PDAC cells. Moreover, OSMR up-regulation induced by LLGL1 inhibition in SW1990 cells depended on the activation of ERK2/Sp1 signaling and subsequent accumulation of Sp1(pThr453) and Pol II at the TATA-less, INR-less but Sp1-binding-site-rich promoter of OSMR, while ectopic expression of LLGL1 in PANC1 cells inactivated ERK2/Sp1 signaling and subsequently reduced the enrichment of Sp1(pThr453) and Pol II at OSMR promoter.CONCLUSIONS: Our studies revealed the novel tumor suppressive role of LLGL1 as a gemcitabine-sensitizing gene in PDAC cells. Loss of LLGL1 resulted in the activation of ERK2/Sp1 signaling and up-regulation of OSMR expression, and ultimately desensitized gemcitabine response in PDAC cells. More importantly, ectopic expression of LLGL1 disrupted such regulatory axis and improved gemcitabine response.Detailed summary in vernacular field only.Detailed summary in vernacular field only.Detailed summary in vernacular field only.Detailed summary in vernacular field only.Zhu, Yinxin.Thesis (Ph.D.) Chinese University of Hong Kong, 2014.Includes bibliographical references (leaves 154-183).Abstracts also in Chinese
Culture-independent and -dependent methods to investigate the diversity of planktonic bacteria in the northern Bering Sea
No full textNational Natural Science Foundation of China [40676002, 40930847, 31070442, 41076130, 41076131]; Emphasis Project Fund of National Scientific and Technical Support Plan [2006BAB18B07]; International Science and Technology Cooperation Program [2008DFA20420]; U.S. National Science Foundation [NSF-OPP-ARC-0454454]; Public Science and Technology Research Funds Projects of Ocean [201105022]Planktonic bacteria are abundant in the Bering Sea. However, very little is known about their diversity and the roles of various bacteria in the ocean. Bacterioplankton diversity in the northern Bering Sea was investigated using a combination of molecular and cultivation-based methods. Community fingerprint analysis using polymerase chain reaction-denaturing gradient gel electrophoresis revealed an apparent difference in the bacterioplankton community composition between sampling locations in the area. The bacterial communities were characterized by two 16S rRNA gene clone libraries for surface and bottom water at shallow station NEC5 (< 60 m in depth) on the continental shelf. Sequences fell into 21 major lineages of the domain Bacteria, including Proteobacteria (Alpha, Beta, Gamma, and Delta), Bacteroidetes, Actinobacteria, Firmicutes, Acidobacteria, Planctomycetes, Verrucomicrobia, Fusobacteria, Chlamydiae, Chloroflexi, Chlorobi, Spirochaetes, Cyanobacteria (or algal chloroplasts), and candidate divisions OP8, OP11, TM6, TM7, and WS3. Significant differences were found between the two clone libraries. Actinobacteria formed the dominant bacterial lineage in both surface and bottom water, and the Alphaproteobacteria was another dominant fraction in surface water. A total of 232 heterotrophic bacterial strains were isolated and 81% showed extracellular proteolytic activity. Phylogenetic analysis revealed that the isolates fell into three bacterial groups, including the Gammaproteobacteria, Actinobacteria, and Firmicutes. The most common genus in both the bacterial isolates and protease-producing bacteria was Pseudoalteromonas. Divergence of bacterial community composition in the northern Bering Sea was mainly characterized by the dominance of Actinobacteria and reflected a bacterial community different from that currently known for marine bacterioplankton communities in other polar regions
Relationships between Arctic and Antarctic Shewanella strains evaluated by a polyphasic taxonomic approach
No full textAs a result of the recent application of DNA-based technologies to investigate bacterial diversity in the polar marine environment, bacterial strains sharing significant 16S rRNA gene sequence similarities were isolated from both the Arctic and the Antarctic sea waters. Three selected marine bacterial isolates (BSw20248, BSw20661 and BSw10166) from two Arctic regions (the Greenland Sea and Canada Basin) and from Antarctica (Prydz Bay) were studied to determine their evolutionary relationships and phylogenetic position using a polyphasic taxonomic approach, including phenotypic characterization and genotypic analyses. These bacterial isolates proved to belong to the same species and were identified as Shewanella frigidimarina. Differences in phenotypic properties observed among them revealed a diversity of ecotypes for adaptation to the changing ecological or geographical conditions. It provided the evidence that Shewanella frigidimarina has a bipolar, or even global, distribution in the marine environment.National Natural Science Foundation of China [40576002, 40676002, 40876097]; High-Tech Research and Development Program of China [2008AA09Z408]; Program for Changjiang Scholars and Innovative Research Team in University [40821063]; Science and Technology Committee of Shanghai, China [052307053]; China's Action Plan for the International Polar Year (IPY
Culture-independent and -dependent methods to investigate the diversity of planktonic bacteria in the northern Bering Sea
No full textMethods for the Viscous Loss Calculation and Thermal Analysis of Oil-Filled Motors: A Review
No full textOil-filled motors (OFMs) are widely used in deep-sea exploration and oil well extraction. During motor operation, the rotor stirs the oil in the air gap, causing viscous loss. Viscous loss affects the temperature distribution inside the motor. Accurately calculating the viscous loss and temperature rise in OFMs can provide a basis for optimizing the motor’s structural design. Motor structural parameters, including the rotor’s outer diameter, air gap, and slot opening, have a significant impact on viscous loss. The working conditions of OFMs, such as rotor speed and environmental temperature, also affect viscous loss. The viscosity of hydraulic oil is highly influenced by temperature, and changes in viscosity can lead to changes in viscous loss. These changes in viscous loss, in turn, alter the temperature distribution. Therefore, the coupling relationship between viscous loss and temperature must be considered. Additionally, when Taylor vortices occur in the fluid, the surface roughness of the rotor also has a significant influence on viscous loss. Currently, both domestic and international research on viscous loss and thermal analysis struggle to simultaneously consider the coupling of viscous loss and the temperature field, rotor surface roughness, and the effect of motor structure. This paper summarizes the methods used in recent years for studying viscous loss and thermal analysis, and puts forward some suggestions for future research on the coupling of the OFM temperature field and viscous loss
