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VIRUS FREE PLANTLET PRODUCTION OF POTATO (Solanum tuberosum L.) THROUGH MERISTEM CULTURE
A Thesis
Submitted to the Department of Plant Pathology
Sher-e-Bangla Agricultural University, Dhaka
In partial fulfilment of the requirements for the degree of
MASTER OF SCIENCE (M.S.)
IN
PLANT PATHOLOGY
SEMESTER: JANUARY-JUNE 2015The experiment was undertaken with a view to establish a protocol for in vitro culture
and plant regeneration using potato meristem as explant collected from potato sprouts
of six popular potato varieties Diamant, Cardinal, L. Rosetta, Granola, Asterix and
BARI ALU 29. The experiment was setup at Tissue culture laboratory of Debiganj
farm, Panchagarh. from March, 2015 to February, 2016. In the present study four
levels of GA3 (100, 200, 300 and 400 ppm) were used to assess the influence of GA3
on sprouting abilities of six popular potato verities. The maximum sprouting
efficiency was observed in 400 ppm GA3 treatment. Seven levels of GA3, 2, 4-D and
Kinetin were used along with fresh MS media to inoculate meristems of potato
sprouts. Maximum size of callus (0.82 cm) was observed in Cardinal inoculated in T3
(0.5 mg/l GA3 +1.0 mg/l 2, 4-D+ 1.5 mg/l KIN). Granola meristem inoculated in
hormonal treatment T2 (0.5mg/l GA3 + 0.5mg/l 2, 4-D + 1.5mg/l KIN) showed the
best results regarding minimum days required to shoot initiation (5.13 days) and the
single shoot length (2.41 cm). Three levels of GA3 treatments (T1- fresh MS, T2-
MS+0.5mg/l GA3 and T3- MS+1.5mg/l) were used to study the subsequent shoot
elongation and plant regeneration of single shoot produced from meristem culture. On
DAS- ELISA test all genotypes showed negative result against virus. Among all
varieties Diamant was the least responsive whereas Granola showed overall better
performance from meristem culture to establishment of plants in field conditions
Going Beyond Counting First Authors in Author Co-citation Analysis
The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
We provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
HEALTHCARE WORKERS’ KNOWLEDGE AND PRACTICES REGARDING EXPANDED PROGRAM ON IMMUNIZATION IN KALASIN, THAILAND
2011-08Primary vaccine failures can occur after high immunization coverage has been achieved. Healthcare workers’ knowledge and practices are influential factors in preventing vaccine failures. Adequate knowledge and practices in the cold chain system are important to keep potency of vaccines and effectiveness of immunization. This cross-sectional study was performed to assess healthcare workers’ knowledge and practices regarding an expanded program on immunization and the cold chain system in Kalasin, Thailand. Data collection methods included interviews, observations and document audit. Ninety primary care units and 117 respondents were selected. Only 55.6% of respondents provided correct answers for questions regarding the immunization schedule of school children and 59.0% of respondents answered correctly for questions about Measles-Mumps-Rubella Vaccine. Healthcare workers in hospitals had better knowledge than healthcare workers in health centers (P<0.001). Healthcare workers who had sufficient training had better knowledge than healthcare workers who had no training (P<0.001). Only 61.1% of primary care units recorded the temperature in the vaccine refrigerator twice a day and 63.3% of primary care units had a flowchart regarding what to do when there is an electric power failure. About 13% of vaccine refrigerators had temperatures outside the recommended range of 2 to 8°C. Practices in hospitals were also better than those in health centers (P=0.001). Knowledge and practices were significantly different between healthcare workers in hospitals and in health centers. Coverage training and regular supervision on vaccine handling and the cold chain system are recommended, especially for health centers in remote areas.departmental bulletin pape
Effect of Foliar Application of Urea on Growth and Yield of Short Durative Lentil Variety (BARI Masur-9)
An experiment was conducted at Regional Agricultural Research Station, Jashore, Bangladesh during rabi season of (2019-2020) to evaluate the effect of foliar application of urea on growth and yield of short durative lentil variety (BARI Masur-9). The experiment was conducted in split plot design with three replications where time of urea spraying, P1= at branching stage, P2= at pod initiation stage were distributed in main plots and doses of urea spraying T1= application of 100% recommended fertilizers as basal except urea, T2= application of 100% recommended fertilizers as basal, T3= application of 75% of urea with 100% of other fertilizers as basal and rest of the 25% of urea by spraying, T4= application of 25% of urea with 100% of other fertilizers as basal and rest of the 75% of urea by spraying, T5= application of 50% of urea with 100% of other fertilizers as basal and rest of the 50% of urea by spraying were distributed in sub plots. The highest plant height, number of pods per plant were obtained from application of 75% of urea with 100% of other fertilizers as basal and rest of the 25% of urea spraying at pod initiation stage. The highest seed yield was also found from application of 75% of urea with 100% of other fertilizers as basal and rest of the 25% of urea spraying at pod initiation stage followed by application of 100% recommended fertilizers as basal and application of 50% of urea with 100% of other fertilizers as basal and rest of the 50% of urea by spraying at branching stage. Plant height, pods per plant and days to maturity showed positive correlation with seed yield. On the other hand days to flower and plants per m2 has no linear relationship with seed yield. The highest marginal benefit cost ratio (3.29) was recorded from application of 75% of urea with 100% of other fertilizers as basal and rest of the 25% of urea by spraying at pod initiation stage. So based on findings to increase the yield potential of BARI Masur-9 foliar application of urea may be a tool and hence application of 75% of urea with 100% of other fertilizers as basal and rest of the 25% of urea by spraying at pod initiation stage may be consider as the best treatment
ELDERLY HEALTH AND ITS CORRELATIONS AMONG UZBEK POPULATION
This study was conducted from November, 2007 to May, 2008 to evaluate the health status of the elderly and correlated factors affecting their health. We collected data from 682 individuals 65 years or older (214 male) from greater Tashkent City in Uzbekistan. The study revealed that 75.4% of the respondents were aged <75 years and that 16.8% of them were not educated. About three-quarters of the respondents rated themselves as ‘healthy.’ The odds ratios (ORs) and 95% confidence intervals (CIs) were estimated through a logistic regression model to determine correlations of elderly health, and adjusted for age and sex. The elderly who had additional income were 2.6 times (95% CI=1.8–4.0) more likely to be healthy. Similarly, those <75 years old (OR=1.5, 95% CI=1.0–2.2), were able to do everyday duties (OR=6.0, 95% CI=3.8–9.3), and those who were married (OR=4.1, 95% CI=1.7–9.7) were also healthy. Conversely, males (OR=0.6, 95% CI=0.4–0.9) and the elderly who were supported by sources other than their own income from work were not healthy. We concluded that having a strong family relationship and adhering to a traditional lifestyle are important for protecting elderly health in Uzbekistan. Substantial financial support and personal care are necessary for the elderly. Creating a healthy atmosphere for them at an individual and family level could ensure a better quality life for the elderly in Uzbekistan
Purification and Characterization of the Antioxidative Substance Produced by Aspergillus oryzae
MEDICINAL PLANTS USED TO TREAT CATTLE DISEASES IN NETRAKONA DISTRICT, BANGLADESH
Most of the rural population of Bangladesh owns one or two cows or goats, which are used either for agricultural purposes (cows) or for selling to generate extra income (goats). For treatment of cattle diseases, they rely on traditional veterinary medicinal practitioners, who use medicinal plants to treat cattle ailments. We conducted an ethnomedicinal survey amongst the traditional veterinary medicinal practitioners in Netrakona district, Bangladesh to identify plants and cattle ailments for which the plants are used. Information on plants were obtained from the traditional cattle healers and individual specimens identified at the Bangladesh National Herbarium. A total of 46 plants distributed into 33 families were identified. The families included Acanthaceae, Alliaceae, Amaryllidaceae, Anacardiaceae, Araceae, Bromeliaceae, Cannabaceae, Convolvulaceae, Cyperaceae, Euphorbiaceae, Fabaceae, Gentianaceae, Lamiaceae, Leguminosae, Menispermaceae, Moraceae, Musaceae, Myrtaceae, Olaceae, Palmae, Piperaceae, Poaceae, Ranunculaceae, Rhamnaceae, Rubiaceae, Rutaceae, Solanaceae, Ulmaceae, Umbelliferae, Urticaceae, Verbenaceae and Zingiberaceae families. Of the families, the highest number of plants (three plants each) belonged to the Anacardiaceae, Euphorbiaceae, Myrtaceae, and Piperaceae families. The various cattle diseases that were claimed to be cured by the medicinal plants included fever, rigor, leg fractures, wound infections, diarrhea, meningitis, helminthiasis, common cold, enlargement of liver, body ache, asthma, retention of urine, mumps, piles, cataract, loss of hair, glossitis, and lack of milk production
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