37 research outputs found
First Indonesian Nasopharyngeal Cancer Whole Epigenome Sequencing Identify Tumour Suppressor CpG Methylation
Get PDFHandoko,1– 3 Marlinda Adham,2,4 Lisnawati Rachmadi,2,5 Demak Lumban Tobing,6 Asmarinah,7 Fadilah,8 Wei Dai,9,10 Anne Wing Mui Lee,10 Soehartati A Gondhowiardjo2,3 1Doctoral Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; 2Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; 3Department of Radiation Oncology, Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia; 4Department of Otorhinolaryngology - Head and Neck Surgery, Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia; 5Department of Anatomical Pathology, Cipto Mangunkusumo National General Hospital, Jakarta, Indonesia; 6Department of Clinical Pathology, Dharmais Cancer Hospital, Jakarta, Indonesia; 7Medical Biology Department, Faculty of Medicine Universitas Indonesia, Jakarta, Indonesia; 8Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia-Bioinformatics Core Facilities, Indonesian Medical Education and Research Institute (IMERI), Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; 9Department of Clinical Oncology, University of Hong Kong, Pokfulam, Hong Kong (SAR), People’s Republic of China; 10Clinical Oncology Center, University of Hong Kong – Shenzhen Hospital, Shenzhen, People’s Republic of ChinaCorrespondence: Soehartati A Gondhowiardjo; Handoko, Email [email protected]: Nasopharyngeal cancer (NPC) is a multifaceted disease characterized by genetic and epigenetic modifications. While Epstein–Barr virus (EBV) infection is a known risk factor, recent studies highlight the significant role of DNA methylation in NPC pathogenesis. Aberrant methylation, particularly at CpG sites, can silence tumour suppressor genes, promoting uncontrolled cell growth. This study aims to analyse the methylation patterns in Indonesian NPC patients through whole-epigenome sequencing.Methods: Seven clinical nasopharyngeal cancer samples were collected and confirmed histopathologically. DNA was extracted, sequenced using Oxford Nanopore technology, and aligned to the GRCh38 human reference genome. Methylation analysis was performed using modkit and statistical analysis with R software. Enriched pathways and processes were identified using ClusterProfiler in R, and gene overlap analysis was conducted.Results: The analysis identified both globally hypermethylated and hypomethylated NPC samples. Key tumour suppressor genes, such as PRKCB, PLCB3, ITGB3, EPHA2, PLCE1, PRKCD, CDKN2A, CDKN2B, RPS6KA2, ERBB4, LRRC4, AKT1, PPP2R5C, and STK11 were frequently hypermethylated and confirmed to have lower expression in an independent NPC transcriptome cohort, suggesting their role in NPC carcinogenesis. Enriched KEGG pathways included PI3K-Akt signalling, ECM–receptor interaction, and focal adhesion. The presence of EBV DNA was confirmed in all samples, implicating its role in influencing methylation patterns.Discussion: This study provides comprehensive insights into the epigenetic landscape of NPC, underscoring the role of CpG methylation in tumour suppressor gene silencing. These findings pave the way for targeted therapies and highlight the need for region-specific approaches in NPC management.Keywords: nasopharyngeal cancer, epigenome, methylation, Epstein–Barr virus, whole-genome sequencin
Principal/Madrasah Management in the Financial Management System
Get PDFManagement is one of the main elements in the success of an activity, there is no field in the field of Education. Student Operational Assistance (BOS) is one part of the school to be managed optimally to obtain maximum common goals. This paper aims to implement the application of financial management in education, especially in BOS. The type of research used includes descriptive qualitative research with an ethnographic approach. The results of the study illustrate that in the implementation of school financial management, especially the management of BOS funds, it is carried out in several stages of planning involving various stakeholders, implementation, monitoring and evaluation
Penerapan model pembelajaran learning cycle (siklus belajar) "5E" berbantuan lks terstruktur untuk meningkatkan penalaran matematika siswa kelas X2 SMA Shalahuddin Malang pada materi geometri dimensi tiga tahun ajaran 2009/2010
No full textMANAJEMEN KEPALA MADRASAH DALAM MENINGKATKAN KINERJA GURU DI MADRASAH IBTIDAIYAH KABUPATEN LAMPUNG TIMUR
Get PDFManajemen kepala madrasah merupakan salah satu faktor yang sangat penting
dalam meningkatkan kinerja guru. Hasil pra survey yang dilakukan di MIM Banarjoyo,
MII Sumberrejo, dan MI Ma’arif 5 Sekampung Kabupaten Lampung Timur dari segi
perencanaan, pengorganisasian, pergerakan, pengawasan dan evaluasi sudah baik.
Sedangkan kinerja guru ketiga madrasah dalam kategori cukup artinya guru belum
maksimal menunjukkan kinerjanya dalam menjalankan tugas dan fungsinya. Adapun
rumusan masalah dalam penelitian ini adalah “Bagaimana perencanaan kepala madrasah
dalam meningkatkan kinerja guru MIM Banarjoyo, MII Sumberrejo, dan MI Ma’arif 5
Sekampung? Bagaimana pengorganisasian kepala madrasah dalam meningkatkan kinerja
guru di MIM Banarjoyo, MII Sumberrejo, dan MI Ma’arif 5 Sekampung? Bagaimana
pergerakan kepala madrasah dalam meningkatkan kinerja guru di MIM Muhammadiyah
Banarjoyo, MII Sumberrejo, dan MI Ma’arif 5 Sekampung? Bagaimana pengawasan
kepala madrasah dalam meningkatkan kinerja guru di MIM Banarjoyo, MII Sumberrejo,
dan MI Ma’arif 5 Sekampung? Bagaimana evaluasi kepala madrasah dalam meningkatkan
kinerja guru di MIM Banarjoyo, MII Sumberrejo, dan MI Ma’arif 5 Sekampung?
Jenis Penelitian ini penelitian kualitatif dengan menggunakan pendekatan
fenomenologi, yaitu mengungkap fenomena yang ada, berupaya mencari, dan menemukan
peristiwa-peristiwa yang mendalam, pendapat, serta isu yang ada, pada objek penelitian.
Alat pengumpul data yang digunakan yaitu observasi, interview/wawancara, dan
dokumentasi.
Hasil penelitian menunjukkan bahwa Manajemen Kepala Madrasah dalam
meningkatkan Kinerja Guru dari segi perencanaan yaitu merumuskan visi, misi dan tujuan
madrasah, merencanakan rapat tahunan di awal tahun dan merencanakan rapat kerja
bulanan, merencanakan pembinaan dan pelatihan guru, mencari para donatur. Dari segi
pengorganisasian yaitu membuat struktur organisasi, menyusun, menetapkan dan
memberikan rincian tugas, mengorganisasikan dan melibatkan personil madrasah dalam
mengambil keputusan. Dari segi pergerakan yaitu memberikan semangat, motivasi,
inspirasi melalui tindakan, pengarahan dan bimbingan secara berkala, memberikan saransaran,
masukan dan perintah dalam pelaksanaan tugas, memberikan fasilitas yang
memadai, memberikan penghargaan, mengembangkan profesi guru. Dari segi pengawasan
yaitu melakukan supervisi, mengawasi dan memonitoring, memberikan sanksi bagi yang
melanggar aturan, menciptakan dan menjaga suasana kondusif. Dari segi evaluasi yaitu
mengevaluasi proses dan hasil pendidikan, portofolio guru, melakukan problem solving,
menghindari dan menanggulangi konflik. Selanjutnya hasil penelitian kinerja guru di
ketiga madrasah tergolong baik. Kepala madrasah dalam meningkatkan kinerja guru dari
masing-masing ketiga madrasah terdapat perbedaan kelebihan, diantaranya; kepala MIM
Banarjoyo dalam meningkatkan kinerja guru ialah melakukan supervisi ke semua personil
madrasah serta adanya para donatur, kepala MII Sumberrejo dalam meningkatkan kinerja
guru ialah melakukan bimbingan dan arahan secara berkala, dan kepala MI Ma’arif 5
Sekampung dalam meningkatkan kinerja guru ialah memberikan penghargaan yang layak
bagi personil yang berprestasi.
Kesimpulan dalam penelitian ini manajemen kepala madrasah dalam
meningkatkan kinerja guru melalui segi perencanaan, pengorganisasian, pergerakan,
pengawasan dan evaluasi dengan hasil baik dan ditunjukkan dengan hasil kinerja guru
diketiga madrasah tergolong baik. Jadi semakin baik manajemen kepala madrasah,
semakin baik pula kinerja guru
Manajemen Kepala Madrasah Dalam Meningkatkan Kinerja Guru Di Era Revolusi Industri 6.0
No full textPenelitian ini meneliti peran manajemen kepala madrasah dalam meningkatkan kinerja guru di era Revolusi Industri 6.0, dengan studi kasus di MIN 1, MIN 2, dan MIN 3 OKU Timur. Latar belakangnya adalah transformasi global dalam pendidikan yang dipicu oleh kemajuan teknologi digital seperti AI, IoT, dan big data, yang menuntut perubahan paradigma dalam pengelolaan lembaga pendidikan, termasuk madrasah. Tujuan penelitian ini adalah untuk mengidentifikasi strategi manajemen yang efektif yang diterapkan oleh kepala madrasah untuk meningkatkan kinerja guru dalam menghadapi tantangan era digital. Metode penelitian menggunakan pendekatan kualitatif dengan desain deskriptif, melibatkan observasi, wawancara, dan dokumentasi untuk mengumpulkan data. Hasil penelitian menunjukkan bahwa kepala madrasah memainkan peran kunci dalam perencanaan, pelaksanaan, dan evaluasi manajemen yang berfokus pada integrasi teknologi dalam pembelajaran dan peningkatan profesionalisme guru. Kesimpulannya, manajemen yang efektif oleh kepala madrasah sangat penting untuk meningkatkan kinerja guru dan mempersiapkan peserta didik dalam menghadapi tantangan global di era Revolusi Industri 6.0. Penelitian ini berkontribusi pada pengembangan manajemen pendidikan Islam dan memberikan wawasan praktis bagi kepala madrasah dan pemangku kepentingan lainnya dalam merancang strategi yang inovatif dan adaptif
Problems with Officials Making Land Deeds in Collecting Fees for Acquisition of Land and Building Rights in Tegal City
No full textIn property sale and purchase transactions, especially for buyers, a tax called BPHTB is imposed. The transaction for the transfer of rights to land and buildings is shown in the deed of sale made by the PPAT and the PPAT is the one who collects the BPHTB, but it is not uncommon to find many transactions for the transfer of rights to land and buildings carried out without using the services of a PPAT, only carried out underhand to avoid costs arising from the transfer of rights, including BPHTB. The purpose of this research is to examine the problems of Land Deed Officials in collecting Land and Building Acquisition Tax, and what are the obstacles and solutions of Land Deed Officials in collecting Land and Building Acquisition Tax. The approach method used in this legal research is an empirical legal approach method or in other words, it is called an empirical normative approach. In relation to the research method used, the author did this by researching legislation, regulations, legal theories and the opinions of leading legal scholars which are secondary data which are then linked to the actual situation, as well as related to problems found in the field related to problems faced by Land Deed Officials in collecting Land and Building Acquisition Tax. The results of the research obtained by the author include the contradiction in the BPHTB tax collection system, which was originally based on a self-assessment system, becoming an official assessment system. The validation process to determine the selling price of land and buildings is not regulated in laws and regulations, so that the process has an impact on changing the BPHTB value for a land object, automatically requiring more time.Keywords: Collection; Land; Selling
Diagnostic and Treatment Problems of Primary Sclerosing Cholangitis
Get PDFPrimary sclerosing cholangitis (PSC) is a chronic cholestatic liver disease with high incidence reported in America (1/100.000 population), but there is still no data available in Indonesia. The best management of this disease is still not found. Thus, in this article author will discuss about the case of PSC in 49-years male patients hospitalized in Cipto Mangunkusumo hospital Jakarta and its treatment
TELEMEDICINE (ONLINE MEDICAL SERVICES) DALAM ERA NEW NORMAL DITINJAU BERDASARKAN HUKUM KESEHATAN (STUDI: PROGRAM TELEMEDICINE INDONESIA/TEMENIN DI RUMAH SAKIT DR. CIPTO MANGUNKUSUMO)
Get PDFHealth is an important basis for the continuity of human life. Every human being has the right to have access to safe, quality, and affordable health services. However, in reality health services in Indonesia still have several obstacles, for examples the lack of health care for remote areas which is hindered by distance and time. Especially during the Corona Virus Disease 2019 (COVID-19) pandemic, a solution is needed to serve public health without violating the regulations that enforce Large-Scale Social Restrictions (PSBB) to prevent the spread of the virus. For this reason, one of the efforts that can be carried out is by “making friends” or utilizing information technology, namely in the form of Telemedicine. Telemedicine is the provision of remote medical services using information and communication technology, which will facilitate access to health services for the public. The purpose of this study was to analyze the application of Telemedicine services in the New Normal Era, especially in the Indonesian Telemedicine Program (TEMENIN) by the Ministry of Health of the Republic of Indonesia at Dr. Cipto Mangunkusumo Hospital. The research method used is normative juridical. The results of the study state that the implementation of Telemedicine services during the New Normal Era mostly refers to the regulations and policies of the Ministry of Health, and is supported by the existence of various Telemedicine applications. However, it does not yet have an official platform from the Government that can guarantee storage and data confidentiality. The author concludes that Telemedicine which already has many benefits, still has several obstacles in it. Furthermore, the author suggests that the Government immediately create a special platform for Telemedicine services, accompanied by laws and regulations or special regulations, which are supported by government agencies or authorities that can oversee its implementation prevent malpractice
Relative Expression of cMyc mRNA in Human Glioma Cells and its Relationship with the Degree of Malignancy
No full textRelative Expression of cMyc85Vol. 5, No. 2, Agustus 2017RESEARCH ARTICLERelative Expression of cMyc mRNA in Human Glioma Cells and its Relationship with the Degree of Malignancy Fidinny I. Hamid,1 Novi S. Hardiany,2* Febrial Hikmah,3 Syaiful Ichwan41Undergraduate Program FM Universitas Indonesia2Department of Biochemistry and Molecular Biology, FM Universitas Indonesia3Biomedical Sciences Program FM Universitas Indonesia4Department of Neurosurgery FM Universitas Indonesia -Dr Cipto Mangunkusumo General Hospital*Corresponding author: [email protected] 10 April 2016; Accepted 23 Agustus 2017DOI: 10.23886/ejki.5.7467AbstractPluripotency of cMyc genes may be one factor for the high glial cell differentiation in glioma thus it can become an alternative therapeutic target. The objective of the study is toanalyze the cMyc mRNA expression and its relationship with the degrees of glioma malignancy. This is a cross-sectional study from 20 glioma samples with different degree of malignancy from Dr. Cipto Mangunkusumo General Hospital, Jakarta during the period of October 2014 until February 2015. The mRNA obtained from glioma samples are converted to cDNA and then amplified. Relative quantification of cMyc mRNA expression is measured by calculating the cycle threshold values of Real Time RT PCR and normalized towards 18s rRNA to predict the relationship between the expression of cMyc and the degree of malignancy. The cMyc expression is increased in accordance with the tumor grade. The cMyc expressions in high grade glioma are 17424.23 folds higher when calibrated to the normal cell, whereas the genes in lower grade tumors are expressed with the rate of 6167.35. Although the values obtained are not statistically significant, this research has strengthened molecular diagnosis, specifically pluripotency as the factor that gives a greater prognostic relevance than the histopathologic diagnosis. As a conclusion, there is a clinical tendency where the relative mRNA expression was higher in in glioma cMyc high degrees compared with low-grade glioma, however it is not statistically significant
EVALUASI IMPLEMENTASI CLINICAL PATHWAY PADA PASIEN INFARK MIOKARD AKUT DI RUMAH SAKIT UMUM PUSAT NASIONAL DR. CIPTO MANGUNKUSUMO JAKARTA
No full textBackground:
Dr. Cipto Mangunkusumo National Center General Hospital (Rumah Sakit
Cipto Mangunkusumo/ RSCM) is a teaching hospital and national referral
hospital, committed to improve international standard of services. One of possible
solution to improve its service equity is by implementing clinical pathway (CP).
There are 8 clinical pathways which were implemented before in RSCM, one of
them is clinical pathway for acute myocardial infarct without complications. This
clinical pathway has been chosen because cardiovascular disease is the leading
cause of death with high risks and high cost. However, there are some obstacles
found in implementing clinical pathway. Therefore, the author want to evaluate
the implementation of clinical pathway in patient with acute myocardial infarct in
RSCM in order to find out the suitability of implementation that has been
performed and to improve the quality of services.
Aim:
The purpose of this research is to evaluate the implementation process of
clinical pathway for patient with acute myocardial infarct in RSCM, toassess the
outcome and obstacle in implementing clinical pathway of acute myocardial
infarct in RSCM.
Method:
This research used descriptive cross sectional study. The analytical unit in this
case is the evaluation of clinical pathway implementation for patient with acute
myocardial infarct that had been performed in RSCM. The data were collected
retrospectively with quantitative and qualitative method. The data were taken
from medical records, throughquestionnaire to the nurses and doctors who were
in charge in ICCU RSCM, and by conducting a Focus Group Discussion (FGD).
Result:
The obedience in implementing clinical pathway for patient with acute
myocardial infarct in RSCM wasn�t good enough. Although the mean was
95,4%, the obedience would be considered 0% if one of the CP wasn�t fully
completed as the stated policy. The lowest obedience was the service in
transferring the patient from Emergency Unit to ICCU, obedience of DPJP (doctor
in charge) and the obedience in performing Corangiography/PCI ranged about
58% to 72.5%.
Length of Stay (LOS) of respondents in this research wasn�t good, the mean
was 8.2 days. By 55% met the standard, but the other 45% had LOS more than 7
daysthat reflected 45% of the respondents did not meet the standard of clinical
pathway for acute myocardial infarct in RSCM. There was 4.3% respondents who
had complication of acute myocardial infarct in RSCM. This research did not find
any mortality but there was 11.6% of the respondents had readmission, and 7 of
them came back because of a schedule for medical procedure and 1 person had
recurrent acute myocardial infarct. The result of chi square test which was used to
evaluate the relationship between LOS with the duration of patient�s transfer to
ICCU, a long time waiting for a medical procedure, and DPJP existence, p value
obtained for each variable >0.05 which means none of them was related with
LOS. The relationship between LOS with the length of waiting time formedical
xiii
procedure resulted p value 1.000, LOS with duration of patient�s transfer to ICCU
resulted p value 0.619 and between LOS with DPJP existence resulted p value
1.000. The case proportion between LOS7days was almost the
same. There were no differencesin, the availability of ICCU, the rapidity e in
giving treatment, and DPJP existence, towards LOS. It was possible that DPJP
visited without signing the verification sheet on patient�s medical record.
There were 27 obstacles and six of them were mostly complained by the
medical staffs in the implementation of clinical pathway for acute myocardial
infarct in RSCM. They were the lack of socialization to all staffs about filling the
CP for
