250 research outputs found
Prolonged darkness reduces liver fibrosis in a mouse model of primary sclerosing cholangitis by miR-200b down-regulation
No full textMelatonin therapy or prolonged exposure to complete darkness reduces biliary hyperplasia and liver fibrosis in bile-duct-ligated (BDL) rats; however, no information exists in primary sclerosing cholangitis (PSC). Thus, we aimed to determine the therapeutic effects of prolonged dark therapy or melatonin administration on hepatic fibrosis in the Mdr2(-/-) mouse model of PSC. Melatonin levels, biliary mass, liver fibrosis, angiogenesis and miR-200b expression were evaluated in wild-type and Mdr2(-/-) mice exposed to darkness or melatonin treatment or in male PSC patient samples and healthy controls. Mdr2(-/-) mice were also treated with miR-200b inhibitor or control before evaluating biliary mass, liver fibrosis, and angiogenesis. After overexpression of arylalkylamine N-acetyltransferase (AANAT; the enzyme regulating melatonin synthesis) or inhibition of miR-200b in cholangiocytes and hepatic stellate cells in vitro, we evaluated angiogenesis and fibrosis gene expression. After exposure to darkness or administration of melatonin, Mdr2(-/-) mice show elevated serum melatonin levels and inhibition of biliary mass, along with reduction of liver fibrosis and angiogenesis. miRNA PCR analysis demonstrated that miR-200b expression increased in Mdr2(-/-) mice and PSC patient samples compared with controls and decreased in Mdr2(-/-) mice subjected to dark exposure or melatonin treatment. Inhibition of miR-200b in Mdr2(-/-) ablates biliary proliferation, liver fibrosis, and angiogenesis. In vitro, overexpression of AANAT or inhibition of miR-200b in cholangiocytes and hepatic stellate cells decreased the expression of miR-200b, angiogenesis, and fibrosis genes. Dark therapy or targeting melatonin/miR-200b axis may be important in the management of biliary damage and liver fibrosis in cholangiopathies including PSC.-Wu, N., Meng, F., Zhou, T., Han, Y., Kennedy, L., Venter, J., Francis, H., DeMorrow, S., Onori, P., Invernizzi, P., Bernuzzi, F., Mancinelli, R., Gaudio, E., Franchitto, A., Glaser, S., Alpini G. Prolonged darkness reduces liver fibrosis in a mouse model of primary sclerosing cholangitis by miR-200b down-regulation
An Energy Management System of a Fuel Cell/Battery Hybrid Boat
No full textAll-electric ships are now a standard offering for energy/propulsion systems in boats. In this context, integrating fuel cells (FCs) as power sources in hybrid energy systems can be an interesting solution because of their high efficiency and low emission. The energy management strategy for different power sources has a great influence on the fuel consumption, dynamic performance and service life of these power sources. This paper presents a hybrid FC/battery power system for a low power boat. The hybrid system consists of the association of a proton exchange membrane fuel cell (PEMFC) and battery bank. The mathematical models for the components of the hybrid system are presented. These models are implemented in Matlab/Simulink environment. Simulations allow analyzing the dynamic performance and power allocation according to a typical driving cycle. In this system, an efficient energy management system (EMS) based on operation states is proposed. This EMS strategy determines the operating point of each component of the system in order to maximize the system efficiency. Simulation results validate the adequacy of the hybrid power system and the proposed EMS for real ship driving cycles
Factors influencing Canadian oncology nurses discussing cannabis use with patients experiencing chemotherapy-induced nausea
No full textA descriptive, cross-sectional study was conducted to determine factors influencing Canadian oncology nurses discussing cannabis use with patients experiencing chemotherapy-induced nausea (CIN). A survey invitation and three reminders were sent to 678 members of the Canadian Association of Nurses in Oncology (CANO) between February 8 and April 10, 2022. An educator sent an extra invitation to 131 oncology nurses in Eastern Ontario. The survey was based on the Ottawa Model of Research Use. Twenty-seven opened the link to the survey and 25 responded. Of 25 nurses, 11 (47.8%) correctly answered the knowledge question about the effectiveness of cannabis for CIN. The top three barriers to discussing cannabis use were social stigma, nurses’ lack of knowledge, and lack of guidance within the workplace. All participants identified needing continuing education and written guidance about use of cannabis for CIN. Although few oncology nurses responded to the survey, most indicated feeling inadequately prepared to discuss cannabis use with patients experiencing CIN
Transcription factor Oct-4 expression in bovine nuclear transfer embryos
No full textThis thesis was scanned from the print manuscript for digital preservation and is copyright the author.
Researchers can access this thesis by asking their local university, institution or public library to
make a request on their behalf. Monash staff and postgraduate students can use the link in the References field
Transcription factor Oct-4 expression in bovine nuclear transfer embryos
No full textThis thesis was scanned from the print manuscript for digital preservation and is copyright the author.
Researchers can access this thesis by asking their local university, institution or public library to
make a request on their behalf. Monash staff and postgraduate students can use the link in the References field
State of the Art of Fuel Cells for Ship Applications
No full textFuel cells promise to be far more efficient, produce lower or zero emissions, and operate cleaner than conventional internal-combustion engine and gas turbine. They are already used for transportation application (buses, cars and tramways). Fuel cells can also be an interesting solution for ships power. However the developments of fuel cell systems for ship are in infancy. The only exception is the PEMFC in the submarines. This solution allows obtaining an air-independent propulsion (AIP) system, which has been adopted in several countries. This paper presents a comprehensive review of different fuel cells and their application on ships. The pro and the cons of the use of fuel cell in ship application are discussed particularly in terms of lifetime and cos
Sinusoidal Vibration Test Control of a Switching Mode Power Amplifier -Fed Electrodynamic Shaker
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