1,845,793 research outputs found
Analysis by high-resolution two-dimensional electrophoresis of differentiation-dependent alterations in cytosolic protein pattern of HL-60 leukemic cells
No full textHL-60 leukemic cells were differentiated along the neutrophilic pathway with retinoic acid (RA) or along the monocytic pathway with 1,25-dihydroxyvitamin D3 (1,25(OH)2D3). Using a high-resolution two-dimensional electrophoresis technique and subsequent silver staining, differentiation-dependent changes in cytosolic protein pattern of HL-60 cells were analysed and were compared with the cytosolic protein pattern of human neutrophils. The amount of 64 and 50 out of a total of 632 proteins studied was increased or decreased in RA- and 1,25(OH)2D3-differentiated HL-60 cells, respectively, in comparison to undifferentiated HL-60 cells. Thirty-three of these proteins were similarly altered in RA- and 1,25(OH)2D3-differentiated HL-60 cells. Twenty-two and 25 of the proteins altered in amount in RA- or 1,25(OH)2D3-differentiated HL-60 cells versus undifferentiated HL-60 cells were similarly altered in human neutrophils in comparison to undifferentiated HL-60 cells. Seven and 10 of the proteins altered in amount in RA- or 1,25(OH)2D3-differentiated HL-60 cells had specific equivalents in neutrophil cytosol. Our results show (i) that neutrophilic and monocytic differentiation is associated with decreases and increases in amount of cytosolic proteins; (ii) that both differentiation processes share a common set of alterations; and (iii) are associated with specific alterations in protein amount
HL-1 cells express an inwardly rectifying K+ current activated via muscarinic receptors comparable to that in mouse atrial myocytes
No full textAn inwardly rectifying K^+ current is present in atrial cardiac myocytes that is activated by acetylcholine (I_{KACh}). Physiologically, activation of the current in the SA node is important in slowing the heart rate with increased parasympathetic tone. It is a paradigm for the direct regulation of signaling effectors by the Gβγ G-protein subunit. Many questions have been addressed in heterologous expression systems with less focus on the behaviour in native myocytes partly because of the technical difficulties in undertaking comparable studies in native cells. In this study, we characterise a potassium current in the atrial-derived cell line HL-1. Using an electrophysiological approach, we compare the characteristics of the potassium current with those in native atrial cells and in a HEK cell line expressing the cloned Kir3.1/3.4 channel. The potassium current recorded in HL-1 is inwardly rectifying and activated by the muscarinic agonist carbachol. Carbachol-activated currents were inhibited by pertussis toxin and tertiapin-Q. The basal current was time-dependently increased when GTP was substituted in the patch-clamp pipette by the non-hydrolysable analogue GTPγS. We compared the kinetics of current modulation in HL-1 with those of freshly isolated atrial mouse cardiomyocytes. The current activation and deactivation kinetics in HL-1 cells are comparable to those measured in atrial cardiomyocytes. Using immunofluorescence, we found GIRK4 at the membrane in HL-1 cells. Real-time RT-PCR confirms the presence of mRNA for the main G-protein subunits, as well as for M2 muscarinic and A1 adenosine receptors. The data suggest HL-1 cells are a good model to study IKAch
CLargeron/ORC-HL-PEAT: Release of ORC-HL-PEAT
No full text<p>This version of the land surface model ORCHIDEE is based on the branch of high latitude ORC-HL (also called sometimes MICT with a more recent merge of the TRUNK version). The release ORC-HL-PEAT includes northern peatland which have been added as a new PFT (14th PFT) based on C3 grass with lower productivity, lower root depth and a lower Vcmax. A specific soil tile have been created for these soils. This enhances a calculation of the flux of water through these soils. Specific hydraulic conditions have been applied for these soils (ref. <a href="https://doi.org/10.5194/gmd-2017-141">https://doi.org/10.5194/gmd-2017-141</a>)</p>
<p>This has been used to study the future trends of the change of water table depth of peatlands as well as their methane emissions.</p>
CFD simulation of NASA HL-CRM with powered UHBPR nacelle
No full textembargoed_20260306The subject of the thesis is related to the development of CFD models for the simulation of UHBPR turbofans installed configurations at high-incidence. The High-Lift version of the NASA Common Research Model (CRM-HL) has been analyzed by generating unstructured meshes according to the 4th AIAA CFD High Lift Prediction Workshop (HLPW-4) guidelines. The results achieved using Ansys Fluent commercial software are analyzed. In particular, take-off conditions (ground level, M=0.26) of the High Lift version of the Common Research Model (CRM-HL) have been studied. The data generated at increasing angle of attack have been analysed in terms of flow field and propulsive forces to highlight
the interaction between the engine and the airframe
HL-60 and dHL60 Giemsa Staining.png
No full textRepresentative images showing no contamination in HL-60 cells, nor in dHL60 cells after 4 days under PMA conditions.<br
Regulation of 92-kD gelatinase release in HL-60 leukemia cells
Full text linkMatrix metalloproteinase 9 (MMP-9), also known as 92-kD type IV collagenase/gelatinase, is believed to play a critical role in tumor invasion and metastasis. Here, we report that MMP-9 was constitutively released from the human promyelocytic cell line HL-60 as determined by zymographic analysis. Tumor necrosis factor-alpha (TNF-alpha) enhanced the enzyme release threefold to fourfold and the protein kinase C (PKC) activator and differentiation inducer 12-O-tetradecanoylphorbol-13- acetate (TPA) eightfold to ninefold. Gelatinase induction by TNF-alpha and TPA was inhibited by actinomycin D or cycloheximide, indicating that de novo protein synthesis was required. Neutralizing monoclonal antibodies to TNF-alpha (anti-TNF-alpha) decreased the basal MMP-9 release of these cells. In addition, these antibodies also significantly interfered with the TPA-induced enzyme release. Agents that inhibit TNF-alpha expression in HL-60 cells, such as pentoxifylline and dexamethasone, completely abrogated both the constitutive and TPA-evoked MMP-9 release. Diethyldithiocarbamate, which is known to stimulate TNF-alpha production in HL-60 cells, exerted a positive effect on MMP-9 release in untreated cells but was inhibitory in TPA-treated HL-60 cells. The PKC inhibitor staurosporine at low concentrations (100 ng/mL) caused a significant augmentation of MMP-9 release in untreated cultures that was blocked by the addition of anti-TNF-alpha. High concentrations (2 mumol/L) of staurosporine completely abolished the extracellular enzyme activity both in untreated and TPA-stimulated cells. These results suggest, that TNF- alpha is required for basal and PKC-mediated MMP-9 release in HL-60 leukemia cells. Thus, MMP-9 secretion may be regulated by TNF-alpha not only in a paracrine but also in an autocrine fashion. This may potentiate the matrix degradative capacity of immature leukemic cells in the processes of bone marrow egress and the evasion of these cells into peripheral tissue
Experimental Check of Deceleration of Neutral Beam-Injected Energetic Ions in the HL-2A Tokamak
Full text linkShort pulses of a deuterium neutral beam (NB) with a duration of less than ? 5 ms were co-injected into magnetohydrodynamic (MHD)-quiescent ohmic deuterium plasmas of the HL-2A tokamak to study the variation of the slowing-down time in two different electron temperature environments. Analyses were made for the decay rate of D-D neutrons produced by beam-plasma interaction following NB turn-off, i.e., experimentally observed neutron decay rates were compared with those predicted by a classical slowing-down model. The results suggest the beam ions decelerate without significant loss in the HL-2A tokamak in round terms. When the critical energy for beam ions is higher than the beam injection energy, it seems that a small fraction of the beam ions is lost.journal articl
Performances of the HL (Hyperloop) transport system
No full textThis paper deals with an analysis of performances of the HL (Hyperloop) transport system considered as an advanced transport alternative to the existing APT (Air Passenger Transport) and HSR (High Speed Rail) systems. The considered performances are operational, financial, social and environmental. The operational performance include capacity and quality of service provided to the system’s users-passengers with attributes such as door-to-door travel time consisting of the access and egress time, schedule delay, in-vehicle time, and interchange time. The economic performances embrace the costs and revenues of operating the system. The costs include that for infrastructure, vehicles, traffic management facilities and equipment, and employees. The revenues embrace earnings from pricing users/passengers. The environmental performances include energy consumption and related emissions of GHGs (Green House Gases), and land use. The social performances are considered to be noise and safety. The analytical models of indicators of these performances are developed and applied to the scenario of operating the HL system on the short- to medium-haul travel distances/routes. These are then compared to the corresponding performances of the HSR and APT. This comparison has shown that the HL system may possess some advantages but also disadvantages regarding particular performances.Transport and PlanningOLD Urban and Regional Developmen
- …
