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    (41(2):169-177)Mass Propagation of Oriental Lily “Casa Blanca” by Shoot Tip Culture

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    香水百合之鱗莖,剝去其外圍之鱗片,取約300~400μm含1對葉原體之莖頂,培養於含有0.2 ppm NAA AA、4 ppm BA、3%蔗糖及0.8%洋菜之Murashige and Skoog(1962)(MS)基本鹽類培養基中,經2個月的培養,可形成約1.5cm大小之原球體,此原球體培養於含有0.5ppm NAA、4 ppm BA、3%蔗糖及0.8%洋菜之MS基本鹽類培養基中可行原球體之增殖;而培養於含有0.2 ppm 2,4-D、0.5 ppm BA、3%蔗糖及0.8%洋菜之MS基本鹽類培養基中則可誘導植株形成。為了縮短幼苗生長時間,可將已長有0.2cm小芽之原球體,培養於含有0.1 ppm NAA,2 ppm BA、3%蔗糖之MS基本鹽類液體培養基中,7天內即可獲得鱗片葉6cm之幼苗,將此幼苗移殖於3%蔗糖及0.8%洋菜之MS基本鹽類之培養基中,經7天之生長即可誘導發根。此具有根系之幼苗移殖於泥炭苔等介質中,置於二層40─50%遮光網之簡易塑膠溫室中健化,健化期間以細霧式自動噴霧噴灑葉面,每30分鐘噴3─4秒,2週後去除一層遮光網;約經1個月後,即可將苗行2─5°C之低溫處理以打破休眠,再經1─1.5個月,即可移殖於田間行養球栽培。 Mass propagation of Oriental lily by shoot tip culture was developed for the purpose of accelerating the propagation rate and avoiding the transmission of virus. Bulb scales of “Casa Blanca” containing 1 pair of leaf primordia, measuring about 300-400 im in size, were excised from the bulb and cultrued. The composition of the medium was MS salts supplemented with 0.2 ppm NAA, 4 ppm BA, 3% sucrose and 0.8% phytagar. Protocorms about 1.5 cm in diameter could be produced after 2 months in culture. Significant proliferation of the protocorm could be observed by culturing in the same medium except the concentration of NAA was increased from 0.2 to 0.5 ppm. Plants regeneration could be induced if the protocorm were cultrued in medium contained 0.2 ppm 2, 4-D, 0.5 ppm BA, 3% sucrose, and 0.8% phytagar. To accelerate the growth rate of the regenerated seedlings, protocorms with buds about 0.2cm in length were transferred to liquid medium with 0.1 ppm NAA, 2 ppm BA, and 3% sucrose. The length of bulb scale leaves could reach 6 cm in 7 days under the culture conditions. Further culture of the seedlings in solid medium plus MS salts, 3% sucrose, and 0.8% phytagar promoted the differentiation of root system. The recommended lmonth hardening procedure of the seedlings included the use of peatmoss, 40-50% shading, and frequent mist-spray irrigation. The seedlings could then be treated with low temperature (2-5°C) for 1-1.5 months for breaking dormancy before transplanted to the field

    (38(3):346-352)Anther culture and somaclonal varivation of tuberose (Polianthes tuberosa L.)

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    晚香玉單瓣栽培種(Polianthes tuberosa L . cv “Single”)單核期的花藥,接種於含有l ppm 2,4-D、3%蔗糖及0.8%洋菜之1/2 MS基本鹽類(Na-Fe-EDTA全量)培養基中,可獲得具有胚發生能力之癒合組織(embryogenic callus)。將此癒合組織移植於植物生長調節劑為0.2 ppm NAA及2 ppm kinetin 之1/2 MS 基本鹽類培養基,可獲得擬胚體及再生植株。所獲得35株之再生值株,據調查已開花8株之園藝性狀,顯示有花序增長、花蕾數增加、花徑變大、花瓣數變少、外花被呈粉紅色及葉片大小的差異,此變異或可供為改良晚香玉單瓣種花序短、花蕾數少及切花品質低等缺點之選拔材料。 Anthers of tuberose variety with single-petal flowers was used in this experiment for callus induction and plant regeneration. Anthers with microspores at uninucleate stage were cultured on medium of ½ strength MS basic salts (with full strength of Na-Fe-EDTA) supplemented with lppm 2, 4-D, 3% sucrose and 0.8% phytagar. The produced embryogenic callus was able to differentiate into embryoids and plantlets after transfering to medium containing 0.2ppm NAA and 2ppm kinetin. A total of 35 plant-lets were produced by the procedures described above. Among them, 8 plants grew to bloom showing significant variaton in various floral traits. These included inflorescence length, number of flower buds, flower size and color. It is suggested that the plants produced from tissue culture can be used as a source for varietal improvement

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Variations on the Author

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    “Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship

    Appropriate Similarity Measures for Author Cocitation Analysis

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    We provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis

    Dispelling the Myths Behind First-author Citation Counts

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    We conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more sophisticated methods

    Author Index

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    koamabayili/VECTRON-author-checklist: VECTRON author checklist

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    We have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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