1,721,015 research outputs found

    Use of scanning ion conductance microscopy to guide and redirect neuronal growth cones

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    Scanning ion conductance microscopy has been applied to neuronal growth cones of the leech either to image or to stimulate them. Growth cone advance was recorded in non-contact mode using a 2% ion current decrease criterion for pipette-membrane distance control. We demonstrate effective growth cone remodelling using a 5% criterion (near-scanning). Recurrent line near-scanning aligned growth cone processes along the scan line. The new membrane protrusions, marked by DiI, started a few minutes after scanning onset and progressively grew in thickness. Using scanning patterns suitable for connecting distinct growth cones, new links were consistently developed. Although the underlying mechanism is still a matter for investigation, a mechanical perturbation produced by the moving probe appeared to induce the process formation. Thanks to its deterministic and interactive features, this novel approach to guiding growth cones is a promising way to develop networks of identified neurons as well as link them with artificial structures

    Commentary on "clinical characteristics and adequate treatment of familial adenomatous polyposis combined with desmoid tumors"

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    Despite being extremely rare in the general population, intra-abdominal DTs (also known as mesenteric fibromatosis) represent the most frequent cause of death in patients affected with FAP having undergone total proctocolectomy

    The first-time phenomenon: successful students' mathematical crisis in secondary-tertiary transition

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    The huge difficulties related to the transition from secondary to tertiary mathematics are documented by several official data. The analysis of these difficulties is a main issue in educational research at undergraduate level. It is of particular interest the case of the students who choose mathematics as a major. In fact, for the most part, they are students considered excellent in mathematics during secondary school, they seem to have the cognitive resources to succeed, but, in many cases, they encounter several difficulties during their university experience. Therefore, it appears particularly interesting to study also the affective sources and consequences of these difficulties. With this aim, we developed a qualitative and narrative study focused on students’ reflec- tions about their mathematical difficulties in the university experience

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
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