111,888 research outputs found
Analisi comparata del controllo molecolare dell'induzione alla fioritura: ruolo dei geni OsMADS22/OsMADS47 e SVP/AGL24 rispettivamente in riso e Arabidopsis
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author-bios-SRD-19-0063.R1 – Supplemental material for The Network Structure of Police Misconduct
No full textSupplemental material, author-bios-SRD-19-0063.R1 for The Network Structure of Police Misconduct by George Wood, Daria Roithmayr and Andrew V. Papachristos in Socius</p
Characterization of rem genes involved in the reproductive development of arabidopsis thaliana
Get PDFThe REM transcriptional factor family belongs to the plant-specific B3 DNA binding domain superfamily, in Arabidopsis thaliana this family is composed of 45 genes preferentially expressed during flower and seed development among which only a few members have been associated to a function. We focused our attention on three homologous members of this family, REM34 , REM35 and REM36, which are expressed in the inflorescence meristem and in the earliest stages of flower development. To uncover their role RNA interference lines for the simultaneous silencing of these three genes were analysed. At the same time, a CRISPR/Cas9 approach was chosen in order to generate single and multiple mutants. The RNAi lines and the CRISPR/Cas9 mutants showed an aberrant phyllotaxis, a higher rate of flower production and, only in the RNAi lines, an arrest in the female gametophyte in the FG1/FG2 phase.
YUCCA1 , which is coexpressed with REM34 and its closest homologs YUC4 , and YUC6 were found to be downregulated in these RNAi lines. These genes code for flavin monooxygenase proteins involved in auxin biosynthesis and their mutation or misregulation phenocopies RNAi lines. All these evidence suggest that REM34 , REM35 and REM36 could be involved in the regulation of auxin biosynthesis at the level of the inflorescence and flower meristem
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Crop Reproductive Meristems in the Genomic Era: A Brief Overview
Get PDFModulation of traits beneficial for cultivation and yield is one of the main goals of crop improvement. One of the targets for enhancing productivity is changing the architecture of inflorescences since in many species it determines fruit and seed yield. Inflorescence shape and organization is genetically established during the early stages of reproductive development and depends on the number, arrangement, activities, and duration of meristems during the reproductive phase of the plant life cycle. Despite the variety of inflorescence architectures observable in nature, many key aspects of inflorescence development are conserved among different species. For instance, the genetic network in charge of specifying the identity of the different reproductive meristems, which can be indeterminate or determinate, seems to be similar among distantly related species. The availability of a large number of published transcriptomic datasets for plants with different inflorescence architectures, allowed us to identify transcription factor gene families that are differentially expressed in determinate and indeterminate reproductive meristems. The data that we review here for Arabidopsis, rice, barley, wheat, and maize, particularly deepens our knowledge of their involvement in meristem identity specification
Molecular mechanism of BASIC PENTACYSTEINE factors and MADS-box transcription factor SVP involved in reproductive development in the model species Arabidopsis thaliana
Get PDFThe development of flower meristems and fruits are two extremely important agronomical traits. MADS-domain transcription factors (TFs) are key regulators of these processes and we are focusing on how these TFs and their interacting partners control the regulatory network leading to reproductive meristem formation and fruit development in the model plant Arabidopsis thaliana.
An excellent starting point for this research project is the large amount of information and tools that we and other groups have already generated for one of the key players in both vegetative and reproductive meristem development: the MADS-domain TF SHORT VEGETATIVE PHASE (SVP). SVP acts as a repressor of the floral transition in the vegetative meristem and later as a key factor floral meristem identity factor (Gregis et al., 2013). Moreover we have recently characterized the function of the complex composed of SVP and BASIC PENTACYSTEINE factors (BPCs) (Simonini et al., 2012). BPCs are transcription factors with affinity to GA-repeated sequences, involved in transcription regulation through their interaction with other TFs and chromatin modifiers. We showed that BPC binding sites are important for BPC recruitment to the DNA but also for binding of SVP to nearby MADS-domain binding sites (CArG boxes).
We focus our attention on the molecular mechanisms by which SVP and its interaction partners control their targets. MADS-domain factors have been studied deeply from a genetic point of view and are recognised as key controllers of development in plants. However, little is known about the molecular aspects (DNA binding mechanisms, recruitment of complex members etc.).
We are studying two important interconnected research lines that are related to the regulatory mechanisms that underlay reproductive development: (i) the mechanistic molecular aspects of target gene regulation by SVP and its interacting partners BPCs and (ii) the analysis of bpc multiple mutants combinations phenotype and the characterization of their role in fruit development
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Causes of death and risk factors among HIV-infected persons in the HAART era: analysis of a large urban cohort
No full textWe aimed to examine the clinical outcome in HIV-1-infected patients after more than 10 years of highly active antiretroviral therapy (HAART)
Assessing the role of REM13, REM34 and REM46 during the transition to the reproductive phase in Arabidopsis thaliana
Get PDFREM (reproductive meristem) transcription factors have been proposed as regulators of plant reproductive development mainly based on their specific expression patterns in reproductive structures, but their roles are still largely unknown probably because of their redundancy. We selected three REM genes (REM13, REM34 and REM46) for functional analysis, based on their genome position and/or co-expression data. Our results suggest that these genes have a role in flowering time regulation and may modulate cell cycle progression. In addition, protein interaction experiments revealed that REM34 and REM46 interact with each other, suggesting that they might work cooperatively to regulate cell division during inflorescence meristem commitment. Previous attempts of using co-expression data as a guide for functional analysis of REMs were limited by the transcriptomic data available at the time. Our results uncover previously unknown functions of three members of the REM family of Arabidopsis thaliana and open the door to more comprehensive studies of the REM family, where the combination of co-expression analysis followed by functional studies might contribute to uncovering the biological roles of these proteins and the relationship among them
Využití sociálních médií v B2B prodeji
No full textTato diplomová práce se zabývá tím, jak mohou B2B obchodníci využívat sociální média v prodeji. Na základě systematické rešerše literatury, autor zjistil, že akademici, zkoumající danou problematiku, navrhují další výzkum, a to: v kterých konkrétních krocích se dají využít sociální média v prodeji (Salo, 2017). Autor se na základě toho rozhodl zjistit, jaké sociální sítě, různé technologie a pluginy se dají využít v B2B prodeji - tzv. social sellingu. Social selling se v této práci týká primárně procesu akvizice a okrajově péčí o stávající zákazníky. Autor si vybral kvalitativní průzkum pomocí 10 hloubkových polo-strukturovaných rozhovorů, aby odhalil jak, která sociální média to jsou, tak i motivaci prodejců, proč tato média používat/nepoužívat. Aby autor dodržel správnost vyhodnocení výsledků, data byla analyzována pomocí Tématické analýzy, která v této studii vykrystalizovala 2 hlavní strategické přístupy v social sellingu. Tyto přístupy (tzv. Push a Pull strategie) obsahují praktické příklady a konkrétní aktivity, které mohou prodejci využívat v každodenní praxi. Tyto výsledky jsou prezentovány s důrazem na praktičnost a jednoduchost implementace. Tvoří proto hlavní přínos autorovo výzkumu. V poslední části autor zmiňuje výzvy a manažerská doporučení, které mohou obchodníci využít v každodenním pracovním životě.This diploma thesis focuses on social media usage in B2B sales. Based on the systematic literature review conducted by the author, he has found out that recent researchers (Salo, 2017) suggest further research in the area of how and in which sales phase should various social networking sites, technologies and plugins used. To further fill this research gap, author decided to identify these social media and their usage among B2B salespeople in the so-called social selling process. The social selling process in this thesis applies mainly to acquiring new prospects and tangentially to taking care of existing clients (follow-up step). Author has chosen a qualitative research method via conducting 10 in-depth semi-structured interviews to reveal these instruments as well as motivation of a sales person on why to use social media in the selling process. The collected data was analyzed using Thematic analysis to ensure the right procedure and to identify main themes which crystalized into 2 main strategic approaches in social selling. These approaches (Push and Pull) include practical examples of concrete activities which sales people can use in their daily jobs and are presented with focus on practicality and ease of implementation. These also form the main contribution of author`s research. In the last part, author mentions challenges in social selling and recommended managerial implications for salesforce
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