1,720,958 research outputs found
Distribution in Cuba and Molecular Characterization of the virus SCYLV, SCMV in Saccharum sp. and PRSV-P in Carica Papaya
No full textEntre las enfermedades más importantes en Saccharum sp., se destacan sugarcane yellow leaf virus (SCYLV) y sugarcane mosaic virus (SCMV). En Carica papaya L., papaya ringspot virus (PRSV-P) causa una de las enfermedades más destructivas. El objetivo de esta investigación fue determinar la distribución en Cuba de estos virus y su caracterización molecular. Se realizaron prospecciones y diagnóstico molecular mediante RT-PCR. En el caso de SCMV se realizó secuenciación directa. Se realizaron análisis filodinámicos con 34 secuencias de aislados cubanos de PRSV-P y 107 secuencias de aislados del continente americano e islas del Caribe. SCYLV y PRSV-P se encuentran ampliamente distribuidos en Cuba. De SCYLV se informan la existencia de las razas BRA-PER, CUB y REU, y de SCMV una sola raza. Los aislados cubanos de PRSVP y del continente americano e islas del Caribe mostraron un ancestro común. SCYLV y PRSV-P constituyen un peligro potencial para los agricultores.Fil: Aday Díaz, Osmany. Instituto de Investigaciones de la Caña de Azúcar; CubaFil: Puchades Izaguirre, Yaquelin. Instituto de Investigaciones de la Caña de Azúcar; CubaFil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola.; ArgentinaFil: González Ramírez, José Efraín. Instituto de Investigaciones de Viandas Tropicales; CubaFil: Portal, Orelvis. Universidad Central “Marta Abreu” de Las Villas; Cuba1er Simposio Internacional Protección de PlantasEl CarmenEcuadorUniversidad Técnica de Ambato. Facultad de Ciencias AgropecuariasCentro de Gestión Internacional de Capacitación y Postgrad
Distribution and molecular characterization of the viruses SCYLV, SCMV in Saccharum sp. and PRSV-P in Carica papaya in Cuba
Full text linkEntre las enfermedades más importantes en Saccharum sp., se destacan sugarcane yellow leaf virus (SCYLV) y sugarcane mosaic virus (SCMV). En Carica papaya L., papaya ringspot virus (PRSV-P) causa una de las enfermedades más destructivas. El objetivo de esta investigación fue determinar la distribución en Cuba de estos virus y su caracterización molecular. Se realizaron prospecciones y diagnóstico molecular mediante RT-PCR. En el caso de SCMV se realizó secuenciación directa. Se realizaron análisis filodinámicos con 34 secuencias de aislados cubanos de PRSV-P y 107 secuencias de aislados del continente americano e islas del Caribe. SCYLV y PRSV-P se encuentran ampliamente distribuidos en Cuba. De SCYLV se informa la existencia de las razas BRA-PER, CUB y REU, y de SCMV una sola raza. Los aislados cubanos de PRSV-P y del continente americano e islas del Caribe mostraron un ancestro común. SCYLV y PRSV-P constituyen un peligro potencial para los agricultores.Among the most important diseases in Saccharum spp., sugarcane yellow leaf virus (SCYLV) and sugarcane mosaic virus (SCMV) stand out. In Carica papaya L., papaya ringspot virus (PRSV-P) causes one of the most destructive diseases. The objective of this research was to determine the distribution in Cuba of these viruses and their molecular characterization. Prospecting and molecular diagnosis were carried out by RT-PCR. In the case of SCMV, direct sequencing was performed. Phylodynamic analyzes were performed with 34 sequences from Cuban PRSV-P isolates and 107 sequences from isolates from the American continent and Caribbean islands. SCYLV and PRSV-P are widely distributed in Cuba. From SCYLV the existence of the races BRA-PER, CUB and REU was reported, and from SCMV a single race. The Cuban isolates of PRSV-P and from the American continent and Caribbean islands showed a common ancestor. SCYLV and PRSV-P constitute a potential hazard to farmers.Instituto de Patología VegetalFil: Aday-Díaz, Osmany. Instituto de Investigaciones de la Caña de Azúcar; CubaFil: Puchades Izaguirre, Yaquelin. Instituto de Investigaciones de la Caña de Azúcar; CubaFil: Cabrera Mederos, Dariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: González Ramírez, José Efraín. Instituto de Investigaciones de Viandas Tropicales. Departamento de Virología de Plantas; CubaFil: Portal, Orelvis. Universidad Central “Marta Abreu” de Las Villas. Centro de Investigaciones Agropecuarias. Facultad de Ciencias Agropecuarias; CubaFil: Portal, Orelvis. Universidad Central “Marta Abreu” de Las Villas. Facultad de Ciencias Agropecuarias. Departamento de Biología; Cub
Caracterización de potyvirus en Dioscorea spp. en el Banco de Germoplasma de ñame del INIVIT
No full textEn Cuba
, la presencia de potyvirus en el cultivo del ñame (
Dioscorea
spp.) ha aumentado
debido a que este
se ha venido propagando de forma agámica.
En este trabajo
,
se
caracterizaron los síntomas por especies y se implementaron las técnicas ELISA
-
DASI,
leaf
-
dip
y
RT
-
PCR para el diagnóstico genérico de potyvirus
y
específico del
Y
am mosaic
virus
(YMV)
y
el
Yam mild mosaic virus
(
YMMV). Se realizó un análisis filogenético de los
potyvirus encontrados y finalmente se analizaron arvenses en las áreas de producción de
semilla del INIVIT y sus alrededores. Se formaron 25 grupos síntoma
-
especie, la clorosis
extendida y el mosaico ligero son los síntomas de mayor frecuencia de aparición. Según
la prueba ELISA resultaron positivos a potyvirus 14 grupos síntoma
-
especie, dond
e
Dioscorea trifida
resulta la especie más susceptible ante l
as enfermedades virales
present
es.
En las muestras se encontraron partículas flexuosas que coinciden con el
género de los potyvirus. El RT
-
PCR confirmó la presencia de potyvirus asociados a 12 de
los 14 grupos síntoma
-
especie evaluados.
El análisis Bayesiano sugirió que han existido
multiples introducciones del YMMV en Cuba, no siendo así para el
YMV
. Se identificaron
26 especies de arvenses pertenecientes a 15 familias, 10 de las cuales resultaro
n
positivos a potyvirus según la prueba ELISA.
Las familias
Asteraceae
y
Cyperaceae
resultaron las más susceptibles ante las enfermedades virales presentes
, por lo que
e
xiste gran diversidad de adverses que constituyen hospedantes alternativos de YMV y
YMM
V
.In Cuba, the presence of potyviruses in the cultivation of yams (
Dioscorea
spp.) h
as
increased due to the fact that it has been spreading aggressively. In
this work
,
the
symptoms were characterized by species and the ELISA
-
DASI, leaf
-
dip and RT
-
PCR
techniques were implemented for the
generic diagnosis of potyvirus and
specific
ally for
Yam mosaic virus
(YMV)
and
Yam mild mosaic virus
(YMMV).
A phylogenetic an
alysis of
the potyviruses was carried out and finally weeds were analyzed in the seed production
areas of INIVIT and its surroundings.
Twenty
-
five
symptom
-
species groups were formed,
the extended chlorosis and the light mosaic are the symptoms of higher fr
equency of
appearance. According to the ELISA test, 14 symptom
-
species groups were positive for
potyvirus, where
Dioscorea trifida
is the most susceptible species to present viral
diseases. In the samples, there were flexuous particles that coincide with t
he genus of the
potyviruses. RT
-
PCR confirmed the presence of potyviruses associated with 12 of the 14
symptom
-
species groups evaluated.
The Bayesian analysis suggested that it has been
multiples introduction of YMMV in Cuba, but not for YMV
. We identified
26 weed species
belonging to 15 families, 10 of which were positive for potyviruses according to the ELISA
test.
Asteraceae
and
Cyperaceae
were the most susceptible families to the viral diseases
present
, for which t
here is a great diversity of adversarie
s that constitute alternative hosts
of YMV and YMMV.Facultad de Ciencias Agropecuarias.Departamento de Biologíanon-publishe
Diagnóstico de enfermedades virales pertenecientes al género de los potivirus en los genotipos de ñame Pacala Duclos (Dioscorea alata L.) y Ñame de Guinea (Discorea rotundata Poir.). Aplicación de la corriente eléctrica al saneamiento.
No full textEl cultivo del ñame es una importante fuente de alimento para gran parte de la población en las regiones tropicales. Dentro del género Discorea las especies D. alata L. y D. rotundata Poir. constituyen dos de las más cultivadas en Cuba y en el mundo. En nuestro país se encuentra extendido en las regiones oriental y central. Este cultivo es afectado por varios virus que pertenecen, al menos, a tres familias conocidas, Potyviridae, Caulimoviridae y Bromoviridae.Instituto de Biotecnología de las Plantasnon-publishe
Effect of plantain barrier plants on potyvirus-associated diseases in yam cultivation
No full textYam belongs to a very diverse plant genus (Dioscorea L.) that includes more than 600 species, both wild and cultivated. In Cuba, different species are distributed in the central and eastern regions, where the most widespread cultivars belong to the species water yam (Dioscorea alata L.) and white yam (Dioscorea cayenensis subsp. rotundata (Poir.) J. Miège). The major constraints faced by yam production areas are those caused by viral diseases. In this sense, potyviruses have the greatest economic impact, since they can cause losses of more than 50% in agricultural yields. The movement of propagules between plantations without phytosanitary certification has facilitated the spread of viruses throughout the production areas. Understanding the relationships between the host, virus, vector, and ecosystem is fundamental for successful disease management and for increasing agricultural yields. The objective of the present work was to determine the effect of using virus-free planting material and plantain barriers on the production of two yam cultivars. For this purpose, potyvirus-free certified and non-certified planting material were evaluated inside and outside a live barrier based on plantain ‘Burro CEMSA’. The virus incidence and production of these plants were determined in each of the experiments conducted. It was possible to reduce the field incidence of viral diseases (average infection decreased from 60 to 15%). The barrier also prevented yield losses of 50–57% for both cultivars, compared to the farmer’s usual practices.CITMA, PN131LH001.63, José Efraín González RamírezPeer reviewe
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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