196,141 research outputs found
Dataset for Gidden, Brutschin et. al. 2023
No full textResults from MESSAGEix-GLOBIOM scenarios incorporating direct air capture and institutional governance as assessed in Gidden, Brutschin et. al. 2023 (https://iopscience.iop.org/article/10.1088/1748-9326/acd8d5/meta)
Tracing International Migration in Projections of Income and Inequality Across the Shared Socioeconomic Pathways
No full textThe Shared Socioeconomic Pathways (SSPs) represent five narratives of future development used for climate change research. They include quantified projections of socioeconomic variables such as population, income levels, inequalities, and emissions over the
twenty-first century. The SSP’s population projections embody explicit, pathway-specific
international migration assumptions, which are only implicit in the projections of other
variables. In this contribution, we explicitly quantify the effects of international migration
on income levels and income inequality across and within countries by comparing the
original SSP projections to scenarios of zero migration. Income projections without
migration are obtained by removing two effects of migration on income dynamics:
changes in population size and remittances sent to origin countries. We base our
remittance estimates on migrant stocks derived from bilateral migration flow estimates
obtained from a gravity model. We find that, on average, migration tends to make the
world richer in all SSP narratives. The nature of migration and remittance corridors is
shaped by the specific scenario of future development considered. Depending on the
particular SSP narrative and world region considered, the effects of migration on income
can be substantial, ranging from −5 to +21% at the continental level. We show that
migration tends to decrease income inequality across countries and within country in
most destination countries but does not affect within-country inequality in origin countries. This new set of projections is consistent with the interdisciplinary framework of the
SSPs, which makes it particularly useful for assessing global climate and sustainable
development policy options
Correspondence, Henry M. Stevenson and Culver Gidden, Spring Observations, June 4, 1977
No full texthttps://digitalcommons.usf.edu/fos_records/1905/thumbnail.jp
Supplemental Data for Gidden et al 2025: A prudent planetary limit for geologic carbon storage
No full textBelow are table captions used in the supplementary data of the manuscript:
Table S1. Global storage volumes considering each geospatial risk consideration layer sequentially, starting from the total global technical potential of onshore storage, offshore storage, and both combined as the global total. Summing all exclusions together results in a global planetary limit, provided at the bottom of the table. An overview of the rationale and quantifications of each limit is provided for every risk consideration. Sensitivity values are provided which estimate the difference in storage based on different assumptions compared to our main estimate for each exclusion layer relative to the previous layer in the main analysis. Exclusion layer sensitivities are described in Table S2.
Table S2. Key sensitivities applied to different exclusion layers. Each sensitivity is labeled based on its Risk Consideration, aligning with tabulated values in Table S1. Negative sensitivities result in lower estimates than the central estimates while positive sensitivities result in higher values. Where sensitivities can be binary (included or not), Y (yes) means they have been included and N (no) means they have not been included. Otherwise, numerical values are provided.
Table S3. A review of available literature which estimates either maximum injection depth, minimum injection depth, or estimates both values. Numerical values are harmonized across sources to provide consistent estimates in meters (m). The majority of the literature we assessed finds that maximal storage depth ranges from around 800-3000m. One study claimed storage depth possible in the gulf of Mexico up to 3500m, but noted that it was unclear about this range due to either pressure in the geopressure zone equilibrating with fracture pressure or loss of permeability. The maximum storage depth we could find was from the USGS which uses a boundary of 3962m based on compression requirements. Taken together, and given the large preponderance of the scientific literature, we maintain a central estimate for maximum storage depth of 2500m, but apply a range between 800m and 3500m in our primary analysis to acknowledge and show the uncertainty in this key parameter in our reported results.
Table S4. A review of countries that currently have explicit policies restricting CCS. Expert judgement is used to estimate whether such policies imply major or minor restrictions and to what degree those policies are subject to change.
Table S5. Country-resolved estimates of onshore, offshore, and total carbon storage are provided for: (1) total technical potential (i.e., without any exclusion layers applied), (2) applying all exclusion layers described in Table S1, and (3) prudent storage estimates including only basins with existing oil and gas infrastructure (i.e., in which storage properties of some part of the basin have already been assessed). We additionally provide the IPCC region in which each country is considered. Countries that do not map to IPCC region categories are included at the bottom of the list. The sum of storage potential across all countries results in the planetary limit.
Table S6. IPCC Scenario categories for the scenarios assessed in this analysis. The IPCC uses shorthand labels (e.g., C1, C2, etc.), while we use the temperature outcomes.
Table S7. A mapping table showing which countries are allocated to each IPCC macro region (so called R5 regions)
Super-resolution imaging of proteins in live cells using reversibly interacting peptide pairs
No full textSuper-resolution techniques have revolutionised our ability to observe cellular structures
with significantly higher resolution than traditional microscopy. Despite the
number of super-resolution microscopy techniques available, live cell super-resolution
imaging remains challenging. For example, while Photo-activated localisation microscopy
(PALM) can be used in vivo, it necessitates the direct fusion of a fluorophore
to the protein of interest. This approach can be problematic because a direct fusion
to a fluorescent protein can disrupt the normal function and localisation of the protein
being studied. Moreover, once the fluorescent protein is photobleached, no more data
can be collected from that molecule.
In this thesis, I describe the development and use of LIVE-PAINT, a novel live-cell
super-resolution microscopy technique. In LIVE-PAINT, a peptide-protein or peptidepeptide
pair, one fused to the protein of interest and the other to a fluorescent protein,
reversibly interact. When the peptide pair bind, a blink is observed, and the precise
location can be determined. In a few minutes, enough binding events occur to generate
an image of the protein of interest with a resolution of around 20 nanometres.
Initially, this work optimises and applies LIVE-PAINT for diffraction-limited and
super-resolution imaging of proteins within live budding yeast cells. I then demonstrate
that the small peptide tag used to label the protein of interest makes LIVE-PAINT a
valuable tool for imaging proteins that are sensitive to direct fusions to fluorescent
proteins. In addition, I validate that LIVE-PAINT enables replenishment of signal
throughout imaging. This is because the imaging peptide, the peptide-labelled fluorescent
protein, is expressed separately from the target protein, creating a pool of imaging
peptides within the cell that can replenish those that are photobleached during imaging.
I utilise this property of LIVE-PAINT to track moving proteins over long periods of
time.
Subsequently, I describe how I adapted the LIVE-PAINT system to apply this
technique to the more complex environment of live mammalian cells. I show that
LIVE-PAINT successfully yields diffraction-limited and super-resolution images of
proteins located in various organelles. This is the first time that interacting peptide pairs
have been used to facilitate point accumulation for imaging in nanoscale topography
(PAINT) based super-resolution imaging in live mammalian cells. These results are
obtained through both transient transfections of labelled proteins and stably integrated
versions. Through this work I generate several new cell lines which can be shared
with other researchers allowing them to use this technique to gain new insights into the
proteins they study.
Furthermore, this thesis explores improvements to the LIVE-PAINT method. I
demonstrate that peptides as small as 5 residues can be used for LIVE-PAINT imaging.
This will broaden the applicability of LIVE-PAINT to a wider range of proteins
that cannot tolerate modifications. To harness the increased brightness of synthetic
fluorescent dyes compared to fluorescent proteins, I developed mammalian cell lines
expressing a HaloTag fused to a LIVE-PAINT peptide. I show that the exogenous
addition of the binding partner to HaloTag, HaloLigand, labelled with a synthetic dye,
to these cells, enables LIVE-PAINT imaging with synthetic dyes. Lastly, I validate that
LIVE-PAINT can be multiplexed by using orthogonal peptide-protein pairs to image
two proteins concurrently in live cells.
In summary, this thesis presents the development and optimisation of LIVE-PAINT,
an innovative peptide-based super-resolution imaging technique tailored for live cell
imaging. While this work explores select applications of LIVE-PAINT, it is anticipated
that this novel technique will have a broad spectrum of applications
List, St. Marks Bird Observations, Culver Gidden to Henry M. Stevenson, April 1 to May 31, 1971
No full texthttps://digitalcommons.usf.edu/fos_records/1795/thumbnail.jp
Dr. Duane M. Jackson, Morehouse College, July 2011
No full textThis video is a conversation with Dr. Duane M. Jackson. Dr. Jackson talks about his paper, "Recall and the Serial Position Effect: The Role of Primacy and Recency on Accounting Students' Performance." Jackie Daniel, AUC Woodruff Library, is the interviewer
"Reflections on the subject of Emigration from Europe with a view to Settlement in the United States" By M. Carey.
No full text"Reflections on the subject of Emigration from Europe with a view to Settlement in the United States: containing bried sketches of the moral and political character of those states.
By M. Carey, member of the American philosophical, and of the American Antiquarian Society, and author of The Olive Branch, Cindiciae Hibernicae, essays on banking, on political economy, and on internal improvement.
To which are now added the English editor's comments on the subject; together with Important Advice to Emigrants, and Cautions Against Impositions Practiced in the Outports
Dispelling the Myths Behind First-author Citation Counts
No full textWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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