1,721,090 research outputs found
Supplemental material for The START nomogram for individualized prediction of the probability of unfavorable outcome after intravenous thrombolysis for stroke
No full textSupplemental material for The START nomogram for individualized prediction of the probability of unfavorable outcome after intravenous thrombolysis for stroke by Manuel Cappellari, Gianni Turcato, Stefano Forlivesi, Fabio Bagante, Gianfranco Cervellin, Giuseppe Lippi, Bruno Bonetti, Paolo Bovi and Danilo Toni in International Journal of Stroke</p
Reduction of burden of hemolyzed specimens in a large urban emergency department: A real-world, five years’ experience
Full text linkIn vitro hemolysis may jeopardize patient care because tests results generated using unsuitable specimens may lead to inappropriate patient management. The prevalence of hemolyzed specimens is high in the emergency department (ED). We previously showed that collecting blood by means of a closed system entailing manual aspiration of blood instead of using conventional evacuated systems was effective to cut-down by nearly half the rate of hemolysis. Aim of this real world study was to verify whether longterm replacement of standard evacuated blood collection systems may be really effective to reduce the burden of spurious hemolysis. Starting from May 2014 in the ED of our Hospital vacuum tubes were replaced with S-Monovette serum tubes. We compared data about hemolyzed specimens entered in the two years before the implementation of the new device (i.e., 2012 and 2013) and the two years after introducing SMonovette in manual aspiration mode (i.e. 2015 and 2016). The year 2014 was not considered due to mixed data. The rate of hemolyzed specimens decreased from 4.36% to 3.07% with the use of S-Monovette in manual aspiration mode (Chi squared, 183.8; P<0.001). The likelihood of obtaining hemolyzed specimens was hence reduced by approximately 30% (relative risk, 0.707), with an expected economic saving of approximately 510€/year. The results of this real-world study demonstrate that the use of an alternative closed device encompassing manual aspiration for drawing blood from intravenous catheters may reduce hemolyzed samples by approximately 30%, so representing a valuable perspective for safeguarding patient safety and improving ED efficiency
Interference from heterophilic antibodies in troponin testing. Case report and systematic review of the literature
No full textHeterophilic antibodies, comprising both "true" heterophilic antibodies and human anti-mouse antibodies (HAMA), represent an important source of interference in laboratory medicine, thus including cardiospecific troponin(s) testing. We describe the case of a 76-years-old woman with implausible and persistent elevation of cardiospecific troponin I, which was finally attributed to interference from heterophilic antibodies. According to literature data, the frequency of this interference ranges between 0.1 and 3.1%, is almost unpredictable and unsuspected, may involve both cardiospecific troponins I and T, and may virtually affect any type of immunoassay, either one- or two-step. The presence of interfering antibodies should always be suspected when test results do not go hand in hand with the clinics, or with the results of additional radiological and laboratory investigations. Once other causes of interference have been ruled out, test repetition with an alternative assay and removal of interfering antibodies with heterophilic antibodies blocking reagent, normal mouse serum, immobilized protein A column or polyethylene glycol may be advisable. As a simple alternative, measurement of serial dilutions of suspected samples usually shows nonlinearity of test results in the presence of heterophilic antibodies
Hemolyzed specimens: a major challenge for emergency departments and clinical laboratories
No full textThe term hemolysis designates the pathological process of breakdown of red blood cells in blood, which is typically accompanied by varying degrees of red tinge in serum or plasma once the whole blood specimen has been centrifuged. Hemolyzed specimens are a rather frequent occurrence in laboratory practice, and the rate of hemolysis is remarkably higher in specimens obtained in the Emergency Department (ED) as compared with other wards or outpatient phlebotomy services. Although hemolyzed specimens may reflect the presence of hemolytic anemia, in most cases they are due to preanalytical sources related to incorrect procedures or failure to follow procedures for collection, handling and storage of the samples; some of these are typical of the ED. Since hemolyzed specimens are often an important cause of relationship, economic, organizational and clinical problems between the ED and the clinical laboratory, it is essential to develop effective processes for systematically identifying unsuitable specimens (e. g. by using the hemolysis index), differentiating in vitro from in vivo hemolysis, troubleshooting the potential causes, and maintaining good relations between the clinical laboratory and the ED
E-Cigarettes and Cardiovascular Risk: Beyond Science and Mysticism
No full textCigarette smoking is the most important cause of premature death, and it is currently listed as a major independent risk factor for cardiovascular disease. Because of restrictive measures and widespread control policies, tobacco companies are now using aggressive marketing strategies in favor of smokeless tobacco, including electronic nicotine delivery systems, which are also known as electronic cigarettes or e-cigarettes. Although the regular use of these devices appears less hazardous than traditional cigarettes or other forms of smokeless tobacco, recent studies have shown that various potentially harmful substances, especially nicotine, ultraparticles, and volatile organic compounds, may be effectively inhaled or liberated in exhaled air during repeated e-cigarette puffing. This would enhance the risk of cardiac arrhythmias and hypertension, which may predispose some users to increased risk of cardiovascular events, which may be further magnified by other potential adverse effects such as arrhythmias, increased respiratory, and flow respiratory resistance. Some cases of intoxication have also been described, wherein large amounts of nicotine and other harmful compounds may be effectively absorbed. As the use of e-cigarettes is continuously rising, and it is also considered a potentially effective method for smoking cessation, more focused research is urgently needed to definitely establish the cardiovascular safeness of these devices
The emerging role of biomarkers and bio-impedance in evaluating hydration status in patients with acute heart failure.
No full textThe quantitative and qualitative estimation of total body fl uid
content has proven to be crucial for both diagnosis and prognosis
assessment in patients with heart failure. The aim of
this review is to summarize the current techniques for assessing
body hydration status as well as the principal biomarkers
associated with acute heart failure (AHF). Although clinical
history, physical examination and classical imaging techniques
(e.g., standard radiography and echocardiography)
still represent the cornerstones, novel and promising tools,
such as biomarkers and bio-electrical impedance are achieving
an emerging role in clinical practice for the assessment
of total body fluid content. In the acute setting, the leading
advantages of these innovative methods over device are representedby the much lower invasiveness and the reasonable
costs, coupled with an easier and faster application. This article
is mainly focused on AHF patients, not only because the
overall prevalence of this disease is dramatically increasing
worldwide, but also because it is well-known that their fluid
overload has a remarkable diagnostic and prognostic significance. It is thereby conceivable that the bio-electrical vector
analysis (BIVA) coupled with laboratory biomarkers might
achieve much success in AHF patient management in the
future, especially for assisting diagnosis, risk stratifi cation,
and therapeutic decision-making
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Homocysteine and migraine. A narrative review.
No full textRecent evidence suggests that migraine is associated with an increased risk of cardiovascular disorders, so that it is increasingly hypothesized that this primary form of headache my be linked to thrombotic diseases by some biological pathways and risk factors. Homocysteine, a sulfur-containing molecule, is now recognized as an independent risk factor for a variety of thrombotic disorders, especially ischemic heart disease and stroke. This article is hence aimed to provide an overview of epidemiological evidence about the association between homocysteine and migraine published in cross-sectional, prospective or interventional studies. Overall, the evidence gathered from cross-sectional studies that measured plasma homocysteine levels suggests that the epidemiological link between the plasma concentration of this biomarker and migraine is very weak, at best. Contradictory evidence emerged from interventional studies, in which treatment of hyperhomocysteinemia with folic acid or vitamin B supplementation was effective to lower plasma homocysteine and decrease frequency and/or severity of migraine. The association remains largely speculative, however, since it could not be clearly demonstrated that these two biological effects were directly linked. The only study that has assessed homocysteine in cerebrospinal fluid reported that the concentration of this biomarker in migraine patients was significantly increased compared to controls. Although this evidence must be obviously confirmed in larger trials, some putative mechanisms may support a causal link between increased generation of homocysteine in the brain environment and migraine
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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