169,776 research outputs found
Organic acid extraction from rhizosphere soil: effect of field-moist, dried and frozen samples
No full textThis study investigates the effect of soil
treatment and storage on organic acid extraction.
For this study one clayey-loamy (Typic Udochrept) and
one sandy-loamy (Aquic Ustifluvent) soil were selected
and used to grow Lupinus albus L. plants in a climate
chamber. After 4 weeks the rhizosphere soil was
sampled and divided into five portions: (a) field moist,
no storage; (b) air-dried; (c) oven-dried, (d) field-moist
at +4°C for 8 weeks; (e) field-moist at −20°C for
8 weeks. Organic acid extraction (1:4 w/v) was carried
out for each soil portion both in water and in 10 mM
NaH2PO4. Organic acid concentration was subsequently
determined by reversed-phase high performance
liquid chromatography (HPLC). Oxalic,
fumaric, malonic and α-ketoglutaric acid were identified
in the rhizosphere of both soils but the extractable
concentration was significantly higher in the sandyloamy
soil. For both soils NaH2PO4 extracted significantly
higher organic acid concentrations than water.
Oven drying increased the extractability of organic
acids in both soils. Field moist samples (i.e. where no
storage occurred) of the sandy-loamy soil showed a
similar behaviour than −20° stored samples whereas
the one of the sandy-loamy soil were more close to the
air-dried samples. These results indicate that organic
acid extraction strongly depends on soil storage as
well as on the soil type. Sample storage seems thus to
be a crucial issue for the determination of organic
acids in rhizosphere soil and needs to be considered
prior analysis
"In vitro" action of intact gamma-globulin on granulopoiesis: modulation of GM-CSA production
No full textScreening of humoral suppression of in vitro granulopoiesis by means of serum inhibitor assay for CFU-GM
No full text
COLECISTECTOMIA LAPAROSCOPICA: INDICAZIONI ALLA COLANGIOGRAFIA INTRA-OPERATORIA
No full textL'indicazione assoluta o relativa
alla colangiografia intra-operatoria in corso di
colecistectomia laparoscopica rimane un argomento
dibattuto, in virtù della possibile esecuzione
di ERCP pre-operatoria con finalità sia
diagnostiche che terapeutiche
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Clinical relevance of two in vitro tests based on CFU-GM assay in the study of granulopoietic failure
No full textMitomycin C in highly myopic eyes - Author reply
No full textOphthalmology. 2005 Feb;112(2):208-18; discussion 219.
Mitomycin C modulation of corneal wound healing after photorefractive keratectomy in highly myopic eyes.
Gambato C, Ghirlando A, Moretto E, Busato F, Midena E.
SourceRefractive Surgery Service and Antimetabolite Therapy Research Unit, Department of Ophthalmology, University of Padova, Padova, Italy.
Abstract
PURPOSE: To evaluate the role of topical mitomycin C in corneal wound healing (CWH) after photorefractive keratectomy (PRK) in highly myopic eyes.
DESIGN: Prospective, double-masked, randomized clinical trial.
PARTICIPANTS: Seventy-two eyes of 36 patients affected by high (>7 diopters) myopia.
METHODS: In each patient, one eye was randomly assigned to PRK with intraoperative topical 0.02% mitomycin C application, and the fellow eye was treated with a placebo. Postoperatively, mitomycin C-treated eyes received artificial tears (3 times daily, tapered in 3 months), whereas the fellow eye was treated with fluorometholone sodium 2% and artificial tears (3 times daily, tapered in 3 months).
MAIN OUTCOME MEASURES: Uncorrected visual acuity (UCVA) and best-corrected visual acuity (BCVA), contrast sensitivity, manifest refraction, and biomicroscopy. Contrast sensitivity was determined using the Pelli-Robson chart. Corneal confocal microscopy documented CWH.
RESULTS: Mean follow-up was 18 months (range, 12-36). No side effects or toxic effects were documented. At 12-month follow-up examination, UCVAs (logarithm of the minimum angle of resolution) were 0.4+/-0.48 and 0.5+/-0.53 (P = .03) in mitomycin C-treated eyes and corticosteroid-treated eyes, respectively. At 1 year, corneal haze developed in 20% of corticosteroid-treated eyes, versus 0% of mitomycin C-treated eyes. At 12, 24, and 36 months, corneal confocal microscopy showed activated keratocytes and extracellular matrix significantly more evident in untreated eyes (Ps = 0.004, 0.024, and 0.046, respectively).
CONCLUSION: Topical intraoperative application of 0.02% mitomycin C can reduce haze formation in highly myopic eyes undergoing PRK.
Comment in
Ophthalmology. 2006 Feb;113(2):357; author reply 357-8
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