7,444 research outputs found
TACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation
No full textThe interaction between TACC3 (transforming acidic coiled coil protein 3) and the microtubule polymerase ch-TOG (colonic, hepatic tumor overexpressed gene) is evolutionarily conserved. Loading of TACC3–ch-TOG onto spindle microtubules requires the phosphorylation of TACC3 by Aurora-A kinase and the subsequent interaction of TACC3 with clathrin to form a microtubule binding surface. Whether there is a pool of TACC3–ch-TOG that is independent of clathrin in human cells, and what is the function of this pool, are open questions. Here, we report that TACC3 is recruited to the plus-ends of microtubules by its association with ch-TOG and that this pool is independent of phosphorylation and binding to clathrin. The plus-end binding of TACC3–ch-TOG persists in interphase and we propose that one cellular function of TACC3–ch-TOG is to modulate cell migration. We also describe the distinct subcellular pools of TACC3, ch-TOG and clathrin. TACC3 is often described as a centrosomal protein, but we show that there is no significant population of TACC3 at centrosomes. The delineation of distinct protein pools reveals a simplified view of how these proteins are organized and controlled by post-translational modification
Ph Abadie, B. Bidaut, Ch. Delefîx, F. Fraizy, J.P. Lemonon, F. Martin, J. Massonnet, Ph. Mercier, Initiation à la lecture d'un texte biblique, Lyon, Profac, 1994
No full textGerber Daniel. Ph Abadie, B. Bidaut, Ch. Delefîx, F. Fraizy, J.P. Lemonon, F. Martin, J. Massonnet, Ph. Mercier, Initiation à la lecture d'un texte biblique, Lyon, Profac, 1994. In: Revue d'histoire et de philosophie religieuses, 76e année n°2, Avril-mai 1996. p. 224
Uncovering a new class of reactions in the atmosphere: SN2-type substitution reactions of nitrogen oxides and seawater
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Previous issue date: 2019-06-18Recent studies indicate that nitrogen oxide species in the atmosphere, including NO and ONONO, undergo a new class of S2-type substitution reactions when in contact with seawater and sea spray aerosols.\footnote{P. J. Kelleher, F. S. Menges, J. W. DePalma, J. K. Denton, M. A. Johnson, G. H. Weddle, B. Hirshberg, R. B. Gerber, \textit{J. Phys. Chem. Lett.} \textbf{8}, 4710 (2017).}\footnote{R. B. Gerber, L. McCaslin, N. V. Karimova, \textit{Faraday Discuss.} (Accepted).}\footnote{L. M. McCaslin, M. A. Johnson, R. B. Gerber, (In review)} The reactions of atmospheric nitrogen oxides with seawater play many integral roles in regulating levels of O, OH, NO, and CH, thus directly affecting radiative forcing and global climate. However, the effect of the number of water molecules on the mechanisms for this new group of S2-type reactions of nitrogen oxides and the competition of these processes with hydrolysis have not yet been characterized. Here we present the mechanisms and timescales of S2-type substitution and hydrolysis reactions of NO with seawater in the cluster series NO + Cl + nHO (n=1-5). Previous studies of the cluster NO + Cl + HO provide deep insights into the local behavior of these systems. The presented studies of this cluster with water molecules added one-by-one allows for a detailed understanding of the effects of a solvation shell as it is built, providing a connection between the behavior of these small clusters and atmospherically relevant systems. Vibrational spectroscopic signatures of key intermediates are discussed and compared to recent and ongoing experiments.$^a
Forces in Scanning Probe Methods
No full textGüntherodt HJ, Anselmetti D, Gerber C, Meyer E, eds. Forces in Scanning Probe Methods. NATO Advances Study Institute. Dordrecht, The Netherlands: Kluwer Academic Publishers; 1995
Delayed cerebral ischaemia: the pathological substrate
No full textIschaemic complications both at the level of the cortex and the hypothalamus are well recognised after an aneurysmal subarachnoid haemorrhage. We have studied histological changes in the cortex (53 patients) and hypothalamus (48 patients) in patients who died after an aneurysmal subarachnoid haemorrhage.Cortical ischaemic lesions were demonstrated in 41 of the 53 patients studied. These changes were more common in patients who had impaired control of systemic blood pressure (p=0.0004) and in patients who died gradually (p=0.0003). Hypothalamic lesions were found in 24 of 48 patients studied; 23 of these patients had widespread associated changes in the cerebral cortex. Patients with moderate/severe cortical changes tended to have hypothalamic lesions and it was uncommon for patients with no cortical lesions to have changes in the hypothalamus (p=0.0007).We believe that these histological changes are due to a diffuse microangiopathy which develops slowly after a subarachnoid haemorrhage and affects the cortex and hypothalamus. Because the cortical lesions are widespread we postulate that they may be implicated in the aetiology of the well described psychosocial or cognitive problems in patients who survive a subarachnoid haemorrhage
Energy balance and cancers
No full textEnergy balance results from the exact equilibrium between caloric intake and caloric expenditure. A caloric intake larger than caloric expenditure results in overweight, even obesity, but other determinants, like hormonal dysfunction and/or genetic traits may play a part in obesity syndrome. Obesity, and even overweight, have been recognized as risk factors for the development of cancers. Human epidemiological studies, which have tended to establish the nature of the relationship between energy balance and cancer, are summarized first, with the influence of the various factors which act both on obesity and on cancer risk. Among these factors are the macronutrients responsible for the caloric intake, and some lifestyle factors (physical activity, drinking habits and tobacco use). Second, the animal studies help to distinguish between different relevant factors, and to understand some of the underlying mechanisms. However, the insulin-resistance syndrome, which appears to underlie the relationship between obesity and hormone-dependent cancers, and possibly colon cancer, is only relevant to human physiology because hormonal alterations are part of it. Prevention of hyperinsulinemia, insulin resistance and the accompanying visceral obesity appears to be a major public health task for the prevention of cancers
Nanomechanical sensing in liquid
No full textThis thesis describes advances in the field of nanomechanical sensors operating in liquid. Firstly, a
novel method for measuring nanoscale displacements is presented. Secondly, microscale Chladnifigures
are demonstrated on oscillating cantilevers by means of boundary streaming in the aqueous
environment. Thirdly, the physics of boundary streaming is clarified for the first time. The three
topics are summarized below.
A novel displacement sensor based on a squeezable molecular multilayer
A novel displacement sensor for nano- and micro-electromechanical devices (NEMS and MEMS)
is introduced. The technique is based on a squeezable molecular multilayer, combined with electron
tunneling or with capacitive readout. The main advantage is the predefined alignment of
the electrodes, allowing miniaturization of traditional tunneling and capacitive sensors. Furthermore,
the device can be operated in aqueous solutions. The multilayers consisted of stacked selfassembled
monolayers (SAMs) of mercaptohexadecanoic acid. Capacitive measurements revealed
the dielectric constant of the multilayers, which was ²r = 1:5. Squeezing of a bilayer lead to an
exponential change in a tunnel current, resulting in nanometer displacement sensitivity.
Nanomechanical resonators generating Chladni figures and boundary
streaming
Chladni figures based on nanomechanics in the microfluidic environment are presented. In contrast
to the macroscopic observations in the gaseous environment, nanoparticles were found to move to
the nodes, whereas micron-sized particles moved to the anti-nodes of the vibrating interface. This
opens the door to size-based sorting of particles in microfluidic systems, and to highly parallel and
controlled assembly of biosensors and nanoelectronic circuits.
The physics of boundary streaming
The physics of boundary streaming is revealed for the first time. This vortex flow phenomenon occurs
near all oscillating surfaces in fluid media, therefore affecting operation of cantilever sensors
and other nanomechanical devices with oscillating components. Here, a solution to the Navier-
Stokes equation is obtained by using a series of physical analogies, giving full insight into the
physics of boundary streaming
Les diminutifs basques avec ch
No full textSe presentan formaciones similares a los diminutivos vascos con "ch" en España y América latina. Se dan ejemplosThe author introduces similar formations to the Basque diminutive "ch" in Spain and Latin America. Examples are provide
COUPLING OF THE C-H STRETCH TO LARGE-AMPLITUDE TORSION AND INVERSION MOTIONS: COMPARISON OF CHCH, CHOH AND CHNH
No full textAuthor Institution: Department of Polymer Science and Department of Chemistry, The University of Akron; Department of Chemistry, The University of Akron, OH 44325In each of the title molecules, torsional and inversion tunneling occurs between six equivalent minima. Coupling of these degrees of freedom to the CH stretch occurs via variation of the C-H stretching force constants as a function of the torsional () and inversion () angles. Maps of the couplings have been computed at the MP2/6-311++G(3df,2p) level. Both the single bond CH stretch force constants and the bilinear couplings between CH bonds are presented as a function of and . Although the torsional barriers differ by more than a factor of 20, the torsion-inversion-vibration coupling patterns are very similar for CHNH and CHCH. On the other hand, the torsion-inversion-vibration coupling in the charged species CHOH is much weaker
Reply to the Ch. Lagrange’s note
No full textThe author answers to a Ch. Lagrange’s note who refutes his theory on the diurnal nutation.L’auteur répond à une note de Ch. Lagrange qui réfute sa théorie sur la nutation diurne
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