1,720,991 research outputs found
FIGURE 4 in Three new species of Culex (Melanoconion) (Diptera: Culicidae) from French Guiana based on morphological and molecular data
No full textFIGURE 4. Male genitalia of Culex (Melanoconion) lucackermanni n. sp. A, Gonocoxopodite, lateral aspect; B, gonocoxopodite, mesal aspect; C, paraproct, tergum X and basal plate, lateral views; D, paramere, lateral view; E, lateral plate and aedeagal sclerite, lateral views; F, tergum IX; G, tergum VIII. AeS, aedeagal sclerite; BP, basal plate; dSL, distal division of subapical lobe; Gc, gonocoxite; GC, gonostylar claw; Gs, gonostylus; LP, lateral plate; lsp, lateral setal patch; Ppr, paraproct; pSL, proximal division of subapical lobe; X-Te, tergum X.Published as part of Talaga, Stanislas & Gendrin, Mathilde, 2022, Three new species of Culex (Melanoconion) (Diptera: Culicidae) from French Guiana based on morphological and molecular data, pp. 177-189 in Zootaxa 5205 (2) on page 184, DOI: 10.11646/zootaxa.5205.2.5, http://zenodo.org/record/730585
FIGURE 3 in Three new species of Culex (Melanoconion) (Diptera: Culicidae) from French Guiana based on morphological and molecular data
No full textFIGURE 3. Male genitalia of Culex (Melanoconion) hutchingsae n. sp. A, Gonocoxopodite, lateral aspect; B, gonocoxopodite, mesal aspect; C, paraproct, tergum X and basal plate, lateral views; D, paramere, lateral view; E, lateral plate and aedeagal sclerite, lateral views; F, tergum IX; G, tergum VIII. AeS, aedeagal sclerite; BP, basal plate; dSL, distal division of subapical lobe; Gc, gonocoxite; GC, gonostylar claw; Gs, gonostylus; LP, lateral plate; lsp, lateral setal patch; Ppr, paraproct; pSL, proximal division of subapical lobe; X-Te, tergum X.Published as part of Talaga, Stanislas & Gendrin, Mathilde, 2022, Three new species of Culex (Melanoconion) (Diptera: Culicidae) from French Guiana based on morphological and molecular data, pp. 177-189 in Zootaxa 5205 (2) on page 182, DOI: 10.11646/zootaxa.5205.2.5, http://zenodo.org/record/730585
Culex (Melanoconion) sallumae Talaga & Gendrin 2022, n. sp.
No full textCulex (Melanoconion) sallumae Talaga, n. sp. Zoobank LSID: urn:lsid:zoobank.org:act: C727E9C3-C13F-40A1-9BF4-279DA8F104D3 BIN: BOLD:AER7739. Male. Habitus not examined. Genitalia (Fig. 2A‒G): Tergum VIII with a shallow emargination separating the lateral lobes. Tergum IX lobes small, somewhat triangular in outline, rounded apically, widely separated, bearing 18‒21 setae arranged in 2 irregular rows. Gonocoxite obovoid; ventrolateral setae strongly developed; ventromesal surface with small setae scattered from base to level of subapical lobe, setae stronger basally; lateral surface with a well-developed patch of long setae (lsp) at level of subapical lobe; proximal part of ventrolateral surface without scales. Subapical lobe clearly divided into 2 divisions. Proximal division short, columnar, bearing 2 robust, sinuous, apically hooked setae (setae a and b); seta a slightly shorter and thinner than seta b and inserted slightly basal to seta b; a tight group of 5 enlarged and abruptly pointed setae inserted lateral to proximal division. Distal division long, columnar, with 7 apical setae, 1 long hooked seta (h), 1 short saber-like seta (s) arising close to seta h, 1 relatively long saber-like seta (s), 1 broad, petiolate, striate foliform seta (l), 3 narrow, appressed flattened setae (f) arising from distal side. Gonostylus slender, curved, wider at base, tapering to apex, with a patch of short spicules on dorsal surface extending from basal 0.33 to apical snout, subapical crest weakly distinct before apical snout on ventral side, apical snout forming a small, upturned ridge; gonostylar claw short, leaf-like; 2 small setae near dorsal side before gonostylar claw. Phallosome with lateral plates and aedeagal sclerites equivalent in length; aedeagal sclerite broad, curved in lateral view and broadly connected to base of lateral plate; distal part of lateral plate without median process, sternal and tergal processes present; apical sternal process long and hooked at apex; apical tergal process very long, nearly pointed, directed dorsolaterally; apical margin markedly concave; base of lateral plate with a markedly distinct tergal process. Aedeagal sclerite not connected by dorsal aedeagal bridge. Proctiger elongate; paraproct narrowed distally, expanded basally, crown a row of about 5 or 6 simple blades. Cercal sclerite long and narrow with 1 or 2 cercal setae. Basal plate and paramere as figured. Tergum X shape as shown in Fig. 2C. Etymology. This species is dedicated to Professor Maria Anice Mureb Sallum for her incommensurable work on mosquito taxonomy in South America, and particularly on the Spissipes Section of Culex (Melanoconion) (e.g. Forattini & Sallum 1985; Forattini & Sallum 1995; Sallum & Forattini 1996; Sallum et al. 1997). Bionomics. Nothing is known about the bionomics of Cx. sallumae. Adult males were collected using CDC and CDC UV-light traps placed at 1 m above ground and operated from 1800 to 0600 h along an oxbow section of a rainforest stream in deep shade. Distribution. Culex sallumae is only known from the type locality (Fig. 1A). Type material. Holotype: Adult male in 96% ethanol with dissected genitalia mounted on a microscope slide (specimen numbers ST1#01700, BOLD: FGMOS2885-22), FRENCH GUIANA: Montagnes de la Trinité, Crique Aya (53.41378° W, 4.60310° N, 115 m above sea level), 3-XI-2020, S. Talaga, IPG. Paratypes: Two adult males in alcohol with dissected genitalia mounted on separate microscope slides (specimen numbers ST1#01702, BOLD: FGMOS2887-22 and ST1#01704, BOLD: FGMOS2889-22), same collection data as the holotype, IPG.Published as part of Talaga, Stanislas & Gendrin, Mathilde, 2022, Three new species of Culex (Melanoconion) (Diptera: Culicidae) from French Guiana based on morphological and molecular data, pp. 177-189 in Zootaxa 5205 (2) on pages 179-181, DOI: 10.11646/zootaxa.5205.2.5, http://zenodo.org/record/730585
MosAIC: An annotated collection of mosquito-associated bacteria with high-quality genome assemblies
Full text linkMosquitoes transmit medically important human pathogens, including viruses like dengue virus and parasites such as Plasmodium spp., the causative agent of malaria. Mosquito microbiomes are critically important for the ability of mosquitoes to transmit disease-causing agents. However, while large collections of bacterial isolates and genomic data exist for vertebrate microbiomes, the vast majority of work in mosquitoes to date is based on 16S rRNA gene amplicon data that provides limited taxonomic resolution and no functional information. To address this gap and facilitate future studies using experimental microbiome manipulations, we generated a bacterial Mosquito-Associated Isolate Collection (MosAIC) consisting of 392 bacterial isolates with extensive metadata and high-quality draft genome assemblies that are publicly available, both isolates and sequence data, for use by the scientific community. MosAIC encompasses 142 species spanning 29 bacterial families, with members of the Enterobacteriaceae comprising 40% of the collection. Phylogenomic analysis of 3 genera, Enterobacter, Serratia, and Elizabethkingia, reveal lineages of mosquito-associated bacteria isolated from different mosquito species in multiple laboratories. Investigation into species’ pangenomes further reveals clusters of genes specific to these lineages, which are of interest for future work to test for functions connected to mosquito host association. Altogether, we describe the generation of a physical collection of mosquito-associated bacterial isolates, their genomic data, and analyses of selected groups in context of genome data from closely related isolates, providing a unique, highly valuable resource for research on bacterial colonisation and adaptation within mosquito hosts. Future efforts will expand the collection to include broader geographic and host species representation, especially from individuals collected from field populations, as well as other mosquito-associated microbes, including fungi, archaea, and protozoa
Culex (Melanoconion) hutchingsae Talaga & Gendrin 2022, n. sp.
No full textCulex (Melanoconion) hutchingsae Talaga, n. sp. Zoobank LSID: urn:lsid:zoobank.org:act: 359A3B9D-819C-4BA0-86C7-AC3E46513ECC BIN: BOLD:AEE6759. Culex (Melanoconion) coppenamensis Form 2 (in part) of Sallum & Hutchings (2003) (illustration of gonostylus, species distribution). Male. Habitus not examined. Genitalia (Fig. 3A‒G): Tergum VIII with a shallow V-shaped emargination separating the 2 lateral lobes, with longer setae forming a lateral concentrated setal group. Tergum IX with 2 distinct lobes, shape as shown in Fig. 3F, bearing 33‒35 setae, outer basal setae clearly longer than the others. Gonocoxite globose, outer margin convex, inner margin nearly straight; ventrolateral setae strongly developed; ventromesal surface with small, scattered setae from base to level of distal division of subapical lobe; lateral surface with a well-developed patch of long setae (lsp) at level of subapical lobe, setae longer ventrally; proximal part of ventrolateral surface with numerous scales. Subapical lobe clearly divided into 2 divisions. Proximal division moderately long, columnar, not clearly divided into 2 arms, proximal arm conspicuously shorter than distal arm, each arm bearing 1 long, robust, sinuous, apically hooked seta (setae a and b), both setae equivalent in length and width; a patch of short setae inserted mesally at base of distal surface. Distal division subdivided into inner and outer arms; inner arm with 2 apical setae, 1 long hooked seta (h) and 1 shorter, narrow, saber-like seta (s) inserted in a small tubercle at base of seta h, both h and s arise from separate tubercles at proximal side, 3 or 4 subapical setae, 1 long, wide, apically curved saberlike seta (s) and 3 indistinct, narrow, appressed flattened setae (f) inserted in small tubercles at base of seta s; outer arm long, nearly straight with 1 foliform seta (l) at apex, seta l strongly enlarged, almost symmetrical, striate at base with a well-developed basal expansion. Gonostylus short, strong, with a patch of long spicules at midlength on dorsal surface, distal 0.5 widened and abruptly tapering to apex in lateral view, bearing a conspicuous subapical crest on ventral side restricted to widest part; gonostylar claw long, leaf-like, 2 setae on dorsal side before gonostylar claw, distal seta slightly larger and longer than proximal seta. Phallosome with lateral plates and aedeagal sclerites equivalent in length; aedeagal sclerite narrow, curved in lateral view with anterior margin thickened and sclerotized, narrowly fused to base of lateral plate; distal part of lateral plate with median, sternal and tergal processes; apical median process conical with apex produced into a point tergad, tergal margin of apical process concave; apical sternal process short, somewhat hook-like, pointed, curved laterally; apical tergal process elongate, shorter than apical median process, pointed and directed dorsolaterally; base of lateral plate with short tergal process; aedeagal sclerite not connected by dorsal aedeagal bridge. Proctiger elongate; paraproct narrowed distally, expanded basally, crown a row of about 14 or 15 short simple blades. Cercal sclerite long and narrow with 2 or 3 cercal setae. Basal plate and paramere as figured. Tergum X somewhat rectangular in outline, rounded at apex. Etymology. This species is dedicated to Rosa Sá Gomes Hutchings for her valuable work on the diversity of mosquitoes in the Brazilian Amazon (e.g. Hutchings et al. 2005, 2010, 2013, 2018, 2020), and particularly on species allied to Culex coppenamensis Bonne-Wepster & Bonne, 1920, published in Sallum & Hutchings (2003) and Hutchings & Sallum (2008). Bionomics. Very little is known about the bionomics of Cx. hutchingsae. Immature stages were collected among roots and dead leaves at the edge of a large ground pool in rainforest (Fig. 5A). Water was highly brown-coloured with dissolved plant substances, acidic (pH = 5.9), moderately warm (26.4°C) and with a conductivity of 390 µS/ cm. Immature stages of Cx. hutchingsae were collected together with Anopheles (Anopheles) punctimacula Dyar & Knab, 1906a, Cx. (Mel.) rabelloi Forattini & Sallum, 1987 and Cx. (Mel.) serratimarge Root, 1927b. Distribution. Culex hutchingsae is known from the type locality and Gare Tigre, French Guiana (Fig. 1A). The latter is located at 45 km north-northeast from the type locality, but this area has been flooded since 1994 following the construction of the Petit Saut dam. Specimens of Cx. hutchingsae collected at Gare Tigre on 30 October 1945 were initially identified as Cx. coppenamensis by Floch (1946) and Floch & Abonnenc (1947). More recently, they were regarded as Cx. coppenamensis Form 2 by Sallum & Hutchings (2003) and as Cx. coppenamensis by Talaga et al. (2021). Type material. Holotype: Adult male in 96% ethanol with dissected genitalia mounted on a microscope slide and associated pupal and larval exuviae in 70% ethanol (specimen numbers ST1#01727, BOLD: FGMOS2912- 22), FRENCH GUIANA: Montagnes de la Trinité, Mare Aya (53.41445° W, 4.60289° N, 120 m above sea level), 5-XI-2020, S. Talaga, IPG. Paratypes: Two individualized adult males in 96% ethanol with dissected genitalia mounted on separate microscope slides and individualized associated pupal exuviae in 70% ethanol (specimen numbers ST1#01729, BOLD: FGMOS2914-22 and ST1#01734, BOLD: FGMOS2919-22), same collection data as the holotype, IPG. Other material examined. One male genitalia mounted on a microscope slide (IPG1#00639) and associated pupal and larval exuviae mounted on a separate microscope slide (IPG1#00603), original specimen number N°817 (17A), FRENCH GUIANA: Gare Tigre, 30-X-45, E. Abonnenc, IPG.Published as part of Talaga, Stanislas & Gendrin, Mathilde, 2022, Three new species of Culex (Melanoconion) (Diptera: Culicidae) from French Guiana based on morphological and molecular data, pp. 177-189 in Zootaxa 5205 (2) on pages 181-183, DOI: 10.11646/zootaxa.5205.2.5, http://zenodo.org/record/730585
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Articulations entre réponses locale et systémique dans les défenses antibactériennes de la Drosophile
No full textAn immune system protects organisms against infections. During my PhD,I have focused on the local immune responses of Drosophila and on their links with the systemic immune response. The systemic response is induced in the fat body by the presence of bacteria in the body cavity while the local response is initiated when bacteria accumulate within an epithelial tissue. Some local gut infections however have been shown to also induce systemic responses in the absence of bacteria in the body cavity. Thus, a signal from the gut is detected by the fat body. It has been proposed that bacterial peptidoglycan, passing across the gut epithelium, could be this signal. In the first project of my PhD, I characterized a new, genital, mode of infection that induces both a local response of the genital tract and a systemic fat body response. Experiments based on genital deposition of peptidoglycan reveal that this molecule is the signal that passes from the genital tract to the fat body. Preliminary results suggest that its passage requires transcytosis. In my second project, I investigated the function of PGRP-LA. PGRPs are regulators and effectors of the immune response in animals. A transcriptome analysis in larval tracheae suggests that PGRP-LA is involved in the maintenance of a basal level of immune response in healthy conditions. Preliminary results suggest that PGRP-LA is also required for a localised response of the fat body surrounding the salivary glands. These studies provide new insights into the immune responses of the tracheae and genital tract and into the communication between barrier epithelia, specifically the salivary glands and the genital tract, and the fat bodyLe système immunitaire assure le maintien de l'intégrité de l'organisme, luttant notamment contre les infections. Durant ma thèse, j'ai étudié la réponse immunitaire locale et ses liens avec la réponse systémique chez la Drosophile. La réponse systémique, à l'échelle de l'organisme, est induite dans le corps gras en présence de bactéries dans la cavité générale et la réponse locale a lieu en cas d'accumulation de bactéries au contact d'un épithélium. Certaines infections locales, par voie orale, induisent à la fois une réponse locale et une réponse systémique, en absence de bactéries dans la cavité générale : cela implique l'envoi d'un signal au corps gras par l'intestin. Il a été proposé que ce signal serait le peptidoglycane bactérien diffusant à travers l'intestin. Ma thèse est constituée de deux projets. D'une part, j'ai caractérisé un mode d'infection locale, par voie génitale, induisant une réponse locale et systémique. Par dépôt génital de peptidoglycane, j'ai mis en évidence que cette molécule est le signal induisant la réponse systémique. Selon des données préliminaires, elle diffuserait par transcytose. D'autre part, j'ai étudié la fonction de PGRP-LA. Les PGRP sont des régulateurs et des effecteurs de la réponse immunitaire chez les animaux. L'analyse du transcriptome de trachées larvaires suggère que PGRP-LA participe au maintien d'un niveau basal d'immunité locale. Selon des résultats préliminaires, il serait impliqué dans la réponse du corps gras attenant aux glandes salivaires. Ma thèse apporte donc des informations sur la réponse immunitaire dans les trachées et l'appareil génital et sur la communication entre épithélium génital ou salivaire et corps gra
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