170,039 research outputs found
P0.1, breathing pattern and pressure support ventilation
No full textComment on
P0.1 is a useful parameter in setting the level of pressure support ventilation. [Intensive Care Med. 1995
Design concepts in lumbar total disc arthroplasty
No full textThe implantation of lumbar disc prostheses based on different design concepts is widely accepted. This paper reviews currently available literature studies on the biomechanics of TDA in the lumbar spine, and is targeted at the evaluation of possible relationships between the aims of TDA and the geometrical, mechanical and material properties of the various available disc prostheses. Both theoretical and experimental studies were analyzed, by a PUBMED search (performed in February 2007, revised in January 2008), focusing on single level TDA. Both semi-constrained and unconstrained lumbar discs seem to be able to restore nearly physiological IAR locations and ROM values. However, both increased and decreased ROM was stated in some papers, unrelated to the clinical outcome. Segmental lordosis alterations after TDA were reported in most cases, for both constrained and unconstrained disc prostheses. An increase in the load through the facet joints was documented, for both semi-constrained and unconstrained artificial discs, but with some contrasting results. Semi-constrained devices may be able to share a greater part of the load, thus protecting the surrounding biological structure from overloading and possible early degeneration, but may be more susceptible to wear. The next level of development will be the biomechanical integration of compression across the motion segment. All these findings need to be supported by long-term clinical outcome studies
Image-based biomechanical models of the musculoskeletal system
No full textFinite element modeling is a precious tool for the investigation of the biomechanics of the musculoskeletal system. A key element for the development of anatomically accurate, state-of-the art finite element models is medical imaging. Indeed, the workflow for the generation of a finite element model includes steps which require the availability of medical images of the subject of interest: segmentation, which is the assignment of each voxel of the images to a specific material such as bone and cartilage, allowing for a three-dimensional reconstruction of the anatomy; meshing, which is the creation of the computational mesh necessary for the approximation of the equations describing the physics of the problem; assignment of the material properties to the various parts of the model, which can be estimated for example from quantitative computed tomography for the bone tissue and with other techniques (elastography, T1rho, and T2 mapping from magnetic resonance imaging) for soft tissues. This paper presents a brief overview of the techniques used for image segmentation, meshing, and assessing the mechanical properties of biological tissues, with focus on finite element models of the musculoskeletal system. Both consolidated methods and recent advances such as those based on artificial intelligence are described
Large-scale chromatin remodeling in germinal vesicle bovine oocytes: interplay with gap junction functionality and developmental competence
No full textIn mammals, oocyte acquires a series of competencies sequentially during folliculogenesis that play critical roles at fertilization and early stages of embryonic development. In mouse, chromatin in germinal vesicle (GV) undergoes dynamic changes during oocyte growth and its progressive condensation has been related to the achievement of developmental potential. Cumulus cells are essential for the acquisition of meiotic competence and play a role in chromatin remodeling during oocyte growth. This study is aimed to characterize the chromatin configuration of growing and fully grown bovine oocytes, the status of communications between oocyte and cumulus cells and oocyte developmental potential. Following nuclear staining, we identified four discrete stages of GV, characterized by an increase of chromatin condensation. GV0 stage represented 82% of growing oocytes and it was absent in fully grown oocytes. GV1, GV2, and GV3 represented, respectively, 24, 31, and 45% of fully grown oocytes. Our data indicated a moderate but significant increase in oocyte diameter between GV0 and GV3 stage. By dye coupling assay the 98% of GV0 oocytes showed fully open communications while the number of oocytes with functionally closed communications with cumulus cells was significantly higher in GV3 group than GV1 and GV2. However, GV0 oocytes were unable to progress through metaphase II while GV2 and GV3 showed the highest developmental capability. We conclude that in bovine, the progressive chromatin condensation is related to the sequential achievement of meiotic and embryonic developmental competencies during oocyte growth and differentiation. Moreover, gap-junction-mediated communications between oocyte and cumulus cells could be implicated in modulating the chromatin remodeling process
Promoting Earth Science topics in the primary schools: future teachers at work in the university lab
No full textGoing Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Compilation of a database, specific for the pesticide active substance and their metabolites, comprising the main genotoxicity endpoints
No full textThe terminal residues of pesticide active substances in food and feed commodities need to be duly
identified following the requirements of Commission Regulation (EU) No 283/2013 in accordance with
Regulation (EC) No 1107/2009. This information is necessary to derive the residue definition for the
dietary risk assessment. EFSA initiated in 2009 a work programme to support the preparation of
scientific guidance on the establishment of the residue definition for risk assessment. In 2012, the
Panel on Plant Protection Products and their Residues (PPR Panel) adopted a scientific opinion on the
toxicological relevance of pesticide metabolites for dietary risk assessment. In its opinion, the PPR
Panel also indicated that the application of integrated approaches including the combination of QSAR
models and read across for the genotoxicity assessment of pesticide residues would imply the
availability of a robust database specific for pesticide active substances and their metabolites.
The overall objective of the project is the compilation of a database specific for pesticide active
substances and their metabolites, which is comprising the different genotoxicity endpoints i.e. point
mutations, structural and numerical chromosome aberrations. For each substances and metabolites
(and or impurities when available), data collection, data extraction and data entry has been performed
according to a methodology agreed by EFSA and the consortium. The database represents a practical
tool to complement the in-silico tools i.e. QSAR, grouping and read across for prediction and indication
of the genotoxicity hazard. Moreover, the database is expected to increase the specificity and
sensitivity of the in-silico tools and to enlarge the chemical domains for their applicability
Acute effects of inhaled nitric oxide in adult respiratory distress syndrome.
No full textThis study evaluated the dose-response effect of inhaled nitric oxide (NO) on gas exchange, haemodynamics, and respiratory mechanics in patients with adult respiratory distress syndrome (ARDS). Of 19 consecutive ARDS patients on mechanical ventilation, eight (42\%) responded to a test of 10 parts per million (ppm) NO inhalation with a 25\% increase in arterial oxygen tension (Pa,O2,) over the baseline value. The eight NO-responders were extensively studied during administration of seven inhaled NO doses: 0.5, 1, 5, 10, 20, 50 and 100 ppm. Pulmonary pressure and pulmonary vascular resistance exhibited a dose-dependent decrease at NO doses of 0.5-5 ppm, with a plateau at higher doses. At all doses, inhaled NO improved O2 exchange via a reduction in venous admixture. On average, the increase in Pa,O2, was maximal at 5 ppm NO. Some patients, however, exhibited maximal improvement in Pa,O2 at 100 ppm NO. In all patients, the increase in arterial O2 content was maximal at 5 ppm NO. The lack of further increase in arterial O2 content above 5 ppm partly depended on an NO-induced increase in methaemoglobin. Respiratory mechanics were not affected by NO inhalation. In conclusion, NO doses < or =5 ppm are effective for optimal treatment both of hypoxaemia and of pulmonary hypertension in adult respiratory distress syndrome. Although NO doses as high as 100 ppm may further increase arterial oxygen tension, this effect may not lead to an improvement in arterial O2 content, due to the NO-induced increase in methaemoglobin. It is important to consider the effect of NO not only on arterial oxygen tension, but also on arterial O2 content for correct management of inhaled nitric oxide therapy
Processability and cracking behaviour of novel high-alloyed tool steels processed by Laser Powder Bed Fusion
No full textConcerning tooling applications, Laser Powder Bed Fusion (LPBF) enables new features such as internal cooling channels that can be implemented in cutting or shaping tools. Thus, higher cutting speeds are feasible thanks to the more efficient cooling that could not be obtained by channels fabricated with conventional methods. However, the alloys exploited for the cutting tools production usually contain high levels of carbon, which makes their LPBF processability challenging due to their high crack-susceptibility. In this work, an approach based on the use of basic physical/empirical indicators has been employed to map the processability of six novel high-alloyed tool steel grades. A large experimental campaign with variable energy densities, single and double passes, as well as different focal points was designed. The results exhibit highly dense but cracked parts. In particular, the LPBF processability deteriorates with increasing carbon content, suggesting that mostly chemistry, rather than process parameters, plays a key role in the determination of the LPBF feasibility. The cooling rate, cooling time between 800 °C and 500 °C, equivalent carbon content, solidification interval, martensite start temperature and volumetric energy density were employed as indicators to provide a rapid classification of processability. The work demonstrates that the combined use of the indicators can better explain the cracking behaviour of carbon-containing tool steels. At a screening level, this approach based on complementar use of physical/empirical tools, may significantly shorten the experimental effort during the design of new compositions, especially when dealing with crack susceptible alloys like carbon-containing tool steels
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