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PENGARUH PEMBERIAN ZONA PELUSIDA 3 ( ZP3) KAMBING PADA SPERMATOZOA SAPI TERHADAP ANGKA CLEAVAGE EMBRIO DALAM FERTILISASIIN VITRO
No full textPenelitian ini dilakukan dengan tujuan untuk mengetahui apakah zona pelusida 3 ( ZP3 ) kambing dapat dipakai untuk mempengaruhi kemampuan spermatozoa dalam fertilisasi in vitro ditinjau dari terbentuknya embrio stadium cleavage. Oosit sapi dibagi dalam tiga kelompok yaitu : kontrol ( PO ): Medium fertilisasi + oosit sapi + spermatozoa sapi; perlakuan pemberian serum (PI) : Medium fertilisasi + Foetal Calf Serum ( FCS ) 10 % + oosit sapi + spermatozoa sapi; perlakuan pemberian Zona Pelusida ( ZP3 ) kambing (P2) : Medium fertilisasi + oosit sapi + spermatozoa sapi yang telah diberi ZP3 kambing 10 %. Media fertilisasi in vitro menggunakan Earle's Balanced Salt Solution ( EBSS ) yang diletakkan dalam drop fertilisasi bentuk rosette. Spermatozoa yang telah dilakukan swimp up, diambil sepertiga atasnya dengan mikropipet. Selanjutnya dimasukkan ke dalam tetes pusat roselle yang berisi 50 111 Earle's Balanced Salt Solution ( EBSS ) lalu kembali diinkubasikan ke inkubator C02 dengan kelembaban 95 % dan suhu 36-38°C selama 1 jam untuk kapasitasi. Oosit matang yang mempunyai cumulus onphorus kompak dimasukkan ke dalam masing-masing tetes radial rosette yang herisi 25 111 Earle 's Balanced Salt Solution ( EBSS ). Media fertilisasi yang sudah berisi spermatozoa ditambah oosit matang dimasukkan kembali dalam inkubator C02 selama 48 jam dan tiap 24 jam digoyang. Pada hari ke-2 dan ke-3 tiap-tiap perlakuan diamati serta pada hari ke-4 dijumlah embrio yang mengalami cleavage. Hasil penelitian menunjukkan bahwa angka cleavage pada perlakuan dengan pemberian Zona Pelusida ( ZP3 ) kambing ( P2 ) lebih kecil dibandingkan dengan kontrol ( PO ) dan perlakuan pemberian serum ( PI ) ( P 0,05 ). Kesimpulan dari penelitian ini adalah pemberian Zona Pelusida ( ZP3 ) kambing pada spermatozoa sapi dapat menurunkan angka cleavage embrio pada fertilisasi m vitro dari oosit sapi
PENGARUH PEMBERlAN WNA PELVSIDA 3 (ZP3 ) KAMBING PABA SPERMATOWA SAPI TERHADAP ANGKA CLEAVAGE EMBRIO DALAM FERTILISASIIN VITRO
Full text linkPene!ltian in! d11akukan dengan tUJuan untuk mengetahui apakah Lon3 pclusida :) ( IP3 ) kambing dapat dipakai untuk mernpengaruhi kcmarnpuan spennatozoa dalam tertilisa<;,j m vilm ditinjau dan terbentuknya embrio stadium cleavage.
Oosit sapi dlbagl dalam tlga keiompok Y31tU komrol ( PO ): Medwm fcrtjlisllSj + oosit sapi -,-spennatozoa sapl, perlakuan pcmbenan serum (Pl) -Medium f,;t1.ilisasi + i'tje(o/ Cofl Serum ( FCS ) 10 % -'-OOSII sapl + spermaloLoa sapi: pcrlakuan pcmberian Zona Pe!us-lda ( ZP3 ) karnbmg (P2)· Medium fcnihsasi ~ om,it sap] -,.-spermatozoa sapi yang telah diberi ZP3 kambmg 10 % Media ferti\!sasl m Vitro rncnggunakan I:'ar/e's Bu!anccd Salt So/ullon ( EBSS ) yang dilerakkan dalam drop fertihsasi bentuk rtJw!tle_ Spermatozoa yang telah dilakukan SH'!mp up, diambi! sepeniga atasnya dengan mikropipet Se1anjutnya dlmasukkan ke d!Jam tetes pusat rmetfe yang berisl 50 I.d Far/e '8 Rulanf'r:J ,'lilt Sv!ulwn ( EBSS ) la1u kembali diinkubasrkan ke mkubator CO: dengan kelembaban 95 % dan suhu 36-38-'C selama
I jam untu!.: kapashasl nosh matang yang meJllpunyai cumulus {)ophuru., kompak dimasukkan ke «alam masing-masing it'te~ radial rlHefle yang bcrisi 25 j.tl 1~-arle \Ha/mu:cd Sail .)'o/urum ( FBSS )_ Media fertlilsasi yang sudah bens! spennalOzoa dltambah O()sit matang dimasukkan kembah dalam mkubator CO'] selama 48 Jam dan ti41p 24 jam uigoyaog.
Pada hari ke~2 dan ke-3 t1ap~tiap perlakuan diamatl serta pada han kCM 4 dijumlah embno yang mengalami de(Jva~e.
Hasi! pCf!c1itian mcnuoJukkan bahwa angka deavaf!,c pada perlakuan dengan pcnlberian Zona Pelusida ( 21'3 ) kambing ( P2 ,1eblh kecil dibandlngkan dengan l.:J.lntrol ( PO) dan ptrlakuau pembenan serum ( PI ) ( P < 0.05 ). Sedai1gkttn angka c/euvuj!.e antara perlakuan pcmbcnan serum ( PI) dan ke1ompok kontroi I PO j tldak berbeda nyata ( 17 ;. 0,05 )
Kc~impuian dan peneHtian mi adaJah pemberian Zona Pelusida ( ZP3 ) kaffibing pada spermatozoa sapi dapat menurunkan angka ,."ieavage embrio pada fertibsas! m vitro dad oosit sap
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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