1,720,967 research outputs found
Analisis Potensi Passive Ultrasonic Irrigant Terhadap Larutan Nano Kitosan Molekul Tinggi 0,2% Dikaitkan dengan Indek Virulensi Enterococcus Faecalis Sebagai Model Anti Bakteri pada Patogenesis Infeksi Saluran Akar
No full textE. faecalis is a microorganism that is most often associated with failure of endodontic treatment and in persistent infections. Its ability to form biofilms has long been recognized as one of the virulence factors that can enable these bacteria to be more resistant to phagocytosis, antibodies and antimicrobials. Irrigation measures have an important role in endodontic treatment. Chitosan in dentistry has biocompatibility, biodegradability, bioadhesi and non-toxic properties in human cells. Some studies suggest the use of ultrasound as a means to increase the action of irrigation solutions so that it can help in cleaning complex anatomic areas. The purpose of this study is to see whether the use of passive ultrasonic irrigation as an additional tool that can help high molecular chitosan nano solutions of 0.2% against the E.faecalis virulence index so that it can be used as an alternative to irrigation solutions in the treatment of tooth root canals. In this study the sample used 27 mandibular premolar teeth, which were divided into 3 groups. Furthermore, examining the quality of the effect of test material on the development of E. faecalis seen bacterial growth, biofilm formation, virulence index E. faecalis, root surface canal roughness, and dissolved oxygen. The results showed that there were significant differences in the formation of biofilms based on incubation time, no significant differences in terms of inhibition. E. faecalis, the roughness values in all treatment groups were not significantly different but the impact of irigan preparation with ultrasonic addition in each treatment group with incubation time was significant, the highest average dissolved oxygen yield at 24 hours and 48 hours was high molecular nano chitosan 0, 2% and distilled water, and 72 hours were seen in the NaOCl 2.5% group.E. faecalis merupakan mikroorganisme yang paling sering dihubungkan dengan kegagalan perawatan endodontik dan pada infeksi yang persisten. Kemampuannya dalam membentuk biofilm telah lama ditahui sebagai salah satu faktor virulensi yang dapat memungkinkan bakteri ini lebih resisten terhadap fagositosis, antibodi dan antimikroba. Tindakan irigasi memiliki peranan penting dalam perawatan endodontik. Kitosan di bidang kedokteran gigi memiliki sifat biokompatibilitas, biodegradabilitas, bioadhesi dan tidak toksik pada sel manusia. Beberapa penelitian menyarankan penggunaan ultrasonik sebagai sarana untuk meningkatkan aksi larutan irigasi sehingga dapat membantu dalam membersihkan area anatomi yang kompleks. Tujuan penelitian ini adalah untuk melihat apakah penggunaan passive ultrasonic irrigation sebagai alat tambahan yang dapat membantu larutan nano kitosan molekul tinggi 0,2% terhadap indeks virulensi E.faecalis sehingga dapat digunakan sebagai alternatif larutan irigasi pada perawatan saluran akar gigi. Pada penelitian ini sampel yang digunakan 27 gigi premolar mandibular, yang dibagi menjadi 3 kelompok. Selanjutnya pemeriksaan secara kualitas efek material uji terhadap perkembangan E. faecalis dilihat dari pertumbuhan bakteri, pembentukan biofilm, indek virulensi E. faecalis, kekasaran permukaan saluran akar gigi, dan oksigen terlarut. Hasil penelitian menunjukkan ada perbedaan yang bermakna pada pembentukan biofilm berdasarkan waktu inkubasi, tidak ada perbedaan bermakna dalam hal inhibisi E. faecalis, nilai kekasaran pada semua kelompok perlakukan tidak terdapat perbedaan bermakna namun dampak dari preparasi bahan irigan dengan penambahan ultrasonik pada setiap kelompok perlakuan dengan waktu inkubasi terdapat perbedaan bermakna, hasil rerata oksigen terlarut tertinggi pada 24 jam dan 48 jam adalah nano kitosan molekul tinggi 0,2% dan aquades, serta pada 72 jam terlihat pada kelompok NaOCl 2,5%.128 HalamanTesis Magiste
Analisis Bioaktivitas Bahan Irigasi Ekstrak Buah Lerak (Sapindus Rarak Dc) Terhadap Patogenesis Fusobacterium Nucleatum (Kajian Uji Hidrofobisitas, Uji Enzim Destruktif, dan Uji Ketahanan Fraktur) (Penelitian In Vitro)
No full textIrrigation has an important role in endodontic treatment. The optimal irrigation
material is usually a combination of two or several irrigation solutions to achieve the
purpose of irrigation but until now there has been no irrigation material that meets all
irrigation material requirements. The most frequently used irrigation solution in
endodontic treatment is NaOCl. However, NaOCl solution has the disadvantage of
being cytotoxic to vital tissues, having an unpleasant odor, and demineralization of
dentine which results in loss of mechanical strength of the dentine. Study about lerak
extract in dentistry as an alternative to root canal irrigation has been carried out. Lerak
fruit has been shown to have antibacterial and antifungal. The purpose of this study in
this study will be to test the hydrophobicity and expression of destructive enzymes to
see the potential effects of lerak extract in inhibiting the development of Fusobacterium
nucleatum and dentin strength test to see the clinical effect on the root canal.
The design of this study was an experimental post test only group. Irrigation
extracts of lerak with concentrations of 25%, 12.5% and 6.25%, NaOCl 2.5% + EDTA
17%, saline within 48 hours to be tested for bioactivity, namely hydropobicity activity
test and destructive enzyme expression. The dentin strength test was carried out within
24, 48, 72 hours using a universal Testing machine (UTM).
The results showed that the irrigation extract of lerak had the same properties
as other synthetic irigan solutions to suppress suppressing the destructive enzyme of F.
nucleatum. All concentrations of lerak extract irrigation have the ability to inhibit the
hydropobicity of Fusobacterium nucleatum. The results of the dentin strength test
showed that the concentration of the material and the incubation time did not affect the
value of dentin strength.Irigasi memiliki peran penting dalam perawatan endodontik. Bahan irigasi yang
optimal biasanya merupakan gabungan dari dua atau beberapa larutan irigasi untuk
mencapai tujuan irigasi tetapi hingga saat ini belum ada bahan irigasi yang memenuhi
semua syarat bahan irigasi. Larutan irigasi yang paling sering digunakan dalam
perawatan endodontik adalah NaOCl. Namun, larutan NaOCl memiliki kekurangan
yaitu sifatnya yang sitotoksik terhadap jaringan vital, mempunyai bau yang kurang
enak, dan terjadi demineralisasi pada dentin yang menyebabkan kehilangan kekuatan
mekanik dentin. Penelitian penggunaan buah lerak di bidang kedokteran gigi sebagai
alternatif bahan irigasi saluran akar telah dilakukan. Buah lerak telah terbukti memiliki
antibakteri dan antifungal. Tujuan dari penelitian ini pada penelitian ini akan dilakukan
uji hidrofobisitas dan ekspresi enzim destruktif untuk melihat potensi efek ekstrak buah
lerak dalam menghambat perkembangan bakteri Fusobacterium nucleatum serta uji
ketahanan fraktur untuk melihat pengaruh secara klinis terhadap saluran akar.
Desain penelitian ini adalah eksperimental post test only group. Bahan irigasi
ekstrak buah lerak dengan konsentrasi 25%, 12,5% dan 6,25%, NaOCl 2,5% + EDTA
17%, salin dalam waktu 48 jam untuk diuji bioaktivitas yaitu uji aktifitas hidropobisitas
dan ekspresi enzim destruktif. Uji ketahanan fraktur dilakukan setelah perlakuan
sampel dalam waktu 24, 48 , 72 jam menggunakan universal Testing machine (UTM).
Hasil penelitian menunjukkan bahwa bahan irigasi ekstrak buah lerak memiliki
sifat yang sama dengan larutan irigan sintetik lainnya untuk menekan menekan
ekspresi enzim destruktif F. nucleatum. Semua konsentrasi bahan irigasi ekstrak buah
lerak memiliki kemampuan dalam menghambat aktivitas hidropobisitas F. nucleatum.
Hasil uji ketahanan fraktur menunjukkan bahwa konsentrasi material uji dan waktu
inkubasi tidak mempengaruhi nilai kekuatan gigi.213 HalamanTesis Magiste
Potential of Ethanol Extract Solution of Secang Wood (Caesalpinia Sappan Linn) and Activated Carbon from Coconut Shell Charcoal as Bioactive Caries Detector Dye Material: Analysis of Phytochemical Test, Antioxidant Test, Antimicrobial Test and MMP-1 Inhibitor Test
No full textBackground : Dental caries is the most prevalent infectious disease of the hard dental tissues in Indonesia and worldwide. The management of deep dentin caries lesions requires selective excavation methods to preserve pulp vitality and the integrity of the dentin-pulp complex. Caries Detecting Dye (CDD) plays a crucial role in distinguishing between infected dentin and dentin that can still be preserved. It also serves as an essential diagnostic tool for identifying occlusal caries and crown fractures. However, commercial CDD materials have limitations, such as lacking bioactivity, antibacterial properties, and the potential to cause irritation.
Objective : This study aims to evaluate the potential of ethanol extract from sappan wood (Caesalpinia sappan Linn) combined with activated charcoal derived from coconut shell as a natural, bioactive alternative to conventional CDD. Sappan wood extract contains active compounds such as brazilin, flavonoids, and tannins, known for their antibacterial, anti-inflammatory, and antioxidant properties.
Methods : This was a laboratory experimental study using a post-test only control group design, conducted at the Laboratory of FMIPA Universitas Sumatera Utara and the Faculty of Dentistry, Syiah Kuala University. The study population consisted of permanent premolars and molars with dentin caries not reaching the pulp.
Results : The results showed that the combination of ethanol extract of sappan wood and activated charcoal could accurately differentiate infected and affected dentin. It also demonstrated antibacterial activity against Streptococcus mutans and inhibitory effects on MMP-1, particularly at a 2% concentration, compared to commercial CDD. Thus, this solution can potentially serve as a natural, bioactive, environmentally friendly, and affordable caries-detecting dye.
Conclusion : Ethanol extract of sappan wood contains active compounds (alkaloids, polyphenols, steroids, flavonoids, and brazilin) that provide antibacterial, anti-inflammatory, and antioxidant effects. It holds promise as a safe, bioactive, and natural alternative for use as caries-detecting dye in dentistry.170 PagesTesis Magiste
Efikasi Antibakterial Larutan Irigasi Sodium Hipoklorit dan Kitosan Oligosakarida terhadap Enterococcus Faecalis pada Perawatan Endodontik dengan Instrumentasi yang Berbeda
No full textThe main goal of root canal treatment is to clean and disinfect the root canal system by eliminating bacteria and removal of necrotic tissue. Mechanical instrumentation alone is not enough to clean the root canal, so it is necessary to use irrigating materials to reach the root canal anatomy that cannot be reached by preparation instruments This study aimed to determine the differences in root canal irrigation and instrumentation on the formation of E. faecalis bacterial biofilm and the formation of dentin residue. Twenty-four single canal mandibular premolars which had been extracted for orthodontic needs were divided into four groups. All groups underwent decoronation. Teeth were divided into four groups, namely A (sample irrigated with 2.5% NaOCl and 17% EDTA and prepared with F-One Blue), B (sample irrigated with 2.5% NaOCl and 2% Chitosan Oligosaccharide and prepared with F-One Blue ), C (sample irrigated with 2.5% NaOCl and 17% EDTA and prepared with One-Curve), D (sample irrigated with 2.5% NaOCl and 2% Chitosan Oligosaccharide and prepared with One-Curve). Then, an examination of E. faecalis biofilm and dentin residue was carried out. The data obtained was subjected to a One-way ANOVA statistical test and continued with the LSD Post Hoc test. The One-way ANOVA test results showed that there was a significant difference in the growth of E. faecalis biofilm between the treatment groups. From the research results it was found that Groups B and D had a very good ability to reduce the mass of E. faecalis biofilm Based on the scatter analysis, the group with root canal irrigation using 2.5% NaOCl and 2% COS and instrumentation with F-One Blue showed the ability to eliminate E. faecalis and inhibit the optimal formation of dentine residues.114 HalamanTesis Magiste
Analisis Bioaktivitas Bahan Irigasi Ekstrak Buah Lerak (Sapindus Rarak Dc) Terhadap Patogenesis Fusobacterium Nucleatum (Kajian Uji Hidrofobisitas, Uji Enzim Destruktif, dan Uji Ketahanan Fraktur) (Penelitian In Vitro)
No full textIrrigation has an important role in endodontic treatment. The optimal irrigation
material is usually a combination of two or several irrigation solutions to achieve the
purpose of irrigation but until now there has been no irrigation material that meets all
irrigation material requirements. The most frequently used irrigation solution in
endodontic treatment is NaOCl. However, NaOCl solution has the disadvantage of
being cytotoxic to vital tissues, having an unpleasant odor, and demineralization of
dentine which results in loss of mechanical strength of the dentine. Study about lerak
extract in dentistry as an alternative to root canal irrigation has been carried out. Lerak
fruit has been shown to have antibacterial and antifungal. The purpose of this study in
this study will be to test the hydrophobicity and expression of destructive enzymes to
see the potential effects of lerak extract in inhibiting the development of Fusobacterium
nucleatum and dentin strength test to see the clinical effect on the root canal.
The design of this study was an experimental post test only group. Irrigation
extracts of lerak with concentrations of 25%, 12.5% and 6.25%, NaOCl 2.5% + EDTA
17%, saline within 48 hours to be tested for bioactivity, namely hydropobicity activity
test and destructive enzyme expression. The dentin strength test was carried out within
24, 48, 72 hours using a universal Testing machine (UTM).
The results showed that the irrigation extract of lerak had the same properties
as other synthetic irigan solutions to suppress suppressing the destructive enzyme of F.
nucleatum. All concentrations of lerak extract irrigation have the ability to inhibit the
hydropobicity of Fusobacterium nucleatum. The results of the dentin strength test
showed that the concentration of the material and the incubation time did not affect the
value of dentin strength.Irigasi memiliki peran penting dalam perawatan endodontik. Bahan irigasi yang
optimal biasanya merupakan gabungan dari dua atau beberapa larutan irigasi untuk
mencapai tujuan irigasi tetapi hingga saat ini belum ada bahan irigasi yang memenuhi
semua syarat bahan irigasi. Larutan irigasi yang paling sering digunakan dalam
perawatan endodontik adalah NaOCl. Namun, larutan NaOCl memiliki kekurangan
yaitu sifatnya yang sitotoksik terhadap jaringan vital, mempunyai bau yang kurang
enak, dan terjadi demineralisasi pada dentin yang menyebabkan kehilangan kekuatan
mekanik dentin. Penelitian penggunaan buah lerak di bidang kedokteran gigi sebagai
alternatif bahan irigasi saluran akar telah dilakukan. Buah lerak telah terbukti memiliki
antibakteri dan antifungal. Tujuan dari penelitian ini pada penelitian ini akan dilakukan
uji hidrofobisitas dan ekspresi enzim destruktif untuk melihat potensi efek ekstrak buah
lerak dalam menghambat perkembangan bakteri Fusobacterium nucleatum serta uji
ketahanan fraktur untuk melihat pengaruh secara klinis terhadap saluran akar.
Desain penelitian ini adalah eksperimental post test only group. Bahan irigasi
ekstrak buah lerak dengan konsentrasi 25%, 12,5% dan 6,25%, NaOCl 2,5% + EDTA
17%, salin dalam waktu 48 jam untuk diuji bioaktivitas yaitu uji aktifitas hidropobisitas
dan ekspresi enzim destruktif. Uji ketahanan fraktur dilakukan setelah perlakuan
sampel dalam waktu 24, 48 , 72 jam menggunakan universal Testing machine (UTM).
Hasil penelitian menunjukkan bahwa bahan irigasi ekstrak buah lerak memiliki
sifat yang sama dengan larutan irigan sintetik lainnya untuk menekan menekan
ekspresi enzim destruktif F. nucleatum. Semua konsentrasi bahan irigasi ekstrak buah
lerak memiliki kemampuan dalam menghambat aktivitas hidropobisitas F. nucleatum.
Hasil uji ketahanan fraktur menunjukkan bahwa konsentrasi material uji dan waktu
inkubasi tidak mempengaruhi nilai kekuatan gigi.213 HalamanTesis Magiste
Analisis Biorespon Larutan Nano Kitosan Molekul Tinggi 0,2% Terhadap Sifat Virulensi Enterococcus Faecalis Sebagai Model Antibakteri Patogenesis Infeksi Saluran Akar ( In Vitro )
No full textKeberhasilan perawatan saluran akar dapat dicapai melalui triad endodonsia
yaitu preparasi akses, pembersihan (cleaning and shaping), serta obturasi saluran
akar, yaitu fluid tight seal. Ketidakmampuan dalam melakukan salah satu tahap
tersebut dapat menyebabkan kegagalan perawatan saluran akar yaitu penyembuhan
(Torabinejad, 2002).
Dalam keadaan normal, pulpo dentinal kompleks steril dan terisolasi dari
mikroba rongga mulut karena dilindungi oleh lapisan enamel dan sementum. Bila
dentin terbuka, mikroba berbentuk planktonik dapat memasuki tubulus dentin,
sehingga beresiko terjadinya infeksi pulpa. Beberapa faktor seperti kompleksitas
saluran akar, invasi mikroorganisme kedalam tubulus dan pembentukan smear layer
yang melindungi bakteri mengakibatkan bakteri masih dapat dijumpai dalam tubulus
dentin walaupun sudah dilakukan pembersihan melalui preparasi biomekanis dan
irigasi. (Svensater dan Bergenholtz, 2004: Siquera dan Rocas, 2011).124 HalamanTesis Magiste
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
No full text“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
No full textWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
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