235 research outputs found
Further evidence for amyloid deposition in clinical pancreatic islet grafts
No full textBackground. The reasons for the long-term complete or partial loss of islet graft function are unknown, but there are obviously other reasons than just pure allogeneic graft rejection. Earlier studies have shown that deposition of islet amyloid polypeptide amyloid in transplanted islets may indicate a mechanism for loss of β cells. Materials and Methods. Sections from liver material from four deceased islet-bearing recipients have been scrutinized for the presence of amyloid. Clinical data and certain aspects of the islet graft pathology of these patients have been published previously. RESULT.: With this extended histological analysis, we demonstrate the occurrence of amyloid deposits in islets transplanted into the liver in three of four patients with type 1 diabetes. Conclusion. The finding adds evidence to the assumption that aggregation of islet amyloid polypeptide might be an important cause of progressing β-cell dysfunction in clinically transplanted islets
Amyloid Deposition in Transplanted Human Pancreatic Islets : A Conceivable Cause of Their Long-Term Failure
No full textFollowing the encouraging report of the Edmonton group, there was a rejuvenation of the islet transplantation field. After that, more pessimistic views spread when long-term results of the clinical outcome were published. A progressive loss of the beta-cell function meant that almost all patients were back on insulin therapy after 5 years. More than 10 years ago, we demonstrated that amyloid deposits rapidly formed in human islets and in mouse islets transgenic for human IAPP when grafted into nude mice. It is, therefore, conceivable to consider amyloid formation as one potential candidate for the long-term failure. The present paper reviews attempts in our laboratories to elucidate the dynamics of and mechanisms behind the formation of amyloid in transplanted islets with special emphasis on the impact of long-term hyperglycemia.Original Publication:Arne Andersson, Sara Bohman, L A Hakan Borg, Johan Paulsson, Sebastian Schultz, Gunilla Westermark and Per Westermark, Amyloid Deposition in Transplanted Human Pancreatic Islets: A Conceivable Cause of Their Long-Term Failure, 2008, EXPERIMENTAL DIABETES RESEARCH, (2008), 562985.http://dx.doi.org/10.1155/2008/562985Copyright: Author
Washingtonia filifera seed extracts inhibit the islet amyloid polypeptide fibrils formations and α-amylase and α-glucosidase activity
No full textWashingtonia filifera seeds have revealed to possess antioxidant properties, butyrylcholinesterase and xanthine oxidase inhibition activities. The literature has indicated a relationship between Alzheimer’s disease (AD) and type-2 diabetes (T2D). Keeping this in mind, we have now evaluated the inhibitory properties of W. filifera seed extracts on α-amylase, α-glucosidase enzyme activity and the Islet Amyloid Polypeptide (IAPP) fibrils formation. Three extracts from seeds of W. filifera were evaluated for their enzyme inhibitory effect and IC50 values were calculated for all the extracts. The inhibition mode was investigated by Lineweaver-Burk plot analysis and the inhibition of IAPP aggregate formation was monitored. W. filifera methanol seed extract appears as the most potent inhibitor of α-amylase, α-glucosidase, and for the IAPP fibril formation. Current findings indicate new potential of this extract that could be used for the identification or development of novel potential agents for T2D and AD
Women and Femininity in the Literary Works of Wanda Malecka
No full textTwórczość literacka Wandy Maleckiej poświadcza jej stopniową emancypację intelektualną i uwrażliwienie na kwestie dotyczące pozycji kobiet. Przełom drugiej i trzeciej dekady XIX w. był dla niej okresem poszukiwań formalnych wśród gatunków oświeceniowych: dialogu, bajki, anegdoty. Prezentowany w nich obraz kobiecości pozostawał stereotypowo prześmiewczy. Drugi etap łączy się z powstaniem Wyjątku z historycznego
poematu Wanda, tekstu poetyckiego, wystylizowanego zgodnie z estetyką romantyczną i przynoszącego kreację silnej, niemal androginicznej postaci kobiecej. Wreszcie lata 30. zostały zdominowane przez sentymentalną nowelę, najodważniej dotykającą kwestii nierówności płci.Wanda Malecka’s literary work remains a proof of her gradual acknowledgment of woman role in society. In the 1810’s and 1820’s she was experimenting with different Enlightment era literary genres, such as dialogue, fable and anecdote. The representation of femininity portrayed in those works was consequently mocking. The romantic poem “Wyjątek z historycznego poematu Wanda” marks the second phase, as it brings heroic lead female character, attributed with male features and desires. In the 1830’s the author focused on short sentimental novels, where the issues concerning gender inequalities were most [email protected] Westermark – doktor nauk humanistycznych, pracuje w Instytucie Literatury Polskiej na Wydziale Polonistyki Uniwersytetu Warszawskiego. Zainteresowania badawcze koncentruje wokół historii literatury romantyzmu oraz problematyki komparatystycznego ujmowania prawa i literatury. Autorka monografii „Muszę się trzymać prawa”. Kultura prawna w twórczości Juliusza Słowackiego (Warszawa 2023).Uniwersytet Warszawski[Korespondencja Stanisława Jachowicza z lat 1846–1857], Biblioteka Narodowa, 1846–1857, sygn. 5775.Malecka, Wanda. „Braminka”, Wanda. Tygodnik Polski Płci Pięknej i Literaturze Poświęcony, t. 1, 1821.Malecka, Wanda. „Domownik” (tu: „Tłumaczenia w nowym guście, obrazek obyczajowy”, „Kobieta modna, powiastka z morałem”), Tygodnik Polski, t. 4, 1819.Malecka, Wanda. „Domownik” (tu: „Wieczór rozkoszny, Iwen i Rozamonda” „Dargo i Krymoina”), Wanda. Tygodnik Polski Płci Pięknej i Literaturze Poświęcony, t. 2, 1820.Malecka Wanda. „Ideały i rzeczywistość”, Wianek, 1837.Malecka, Wanda. „Miłość utajona. Powieść prawdziwa”, Tygodnik Polski i Zagraniczny, t. 3, 1818.Malecka, Wanda. „Opuszczona”, Niezapominajki, 1838.Malecka, Wanda. „Teraz dopiero kocham prawdziwie. Szkic z rzeczywistego życia”, Wianek, 1836.Malecka, Wanda. „Wyjątek z historycznego poematu Wanda” wraz ze wstępem prozą „Pieśni Wandy”, Rozmaitości Warszawskie, nr 3, 1826.Malecka, Wanda. „Wyjątek z historycznego poematu Wanda” wraz ze wstępem prozą „Pieśni Wandy”, Wanda. Tygodnik Nadwiślański, nr 4, 1828.Bachórz, Józef. Kowalczykowa, Alina (red.), Słownik literatury polskiej XIX wieku, (Wrocław: Zakład Narodowy im. Ossolińskich, 2009).Franke, Jerzy. Polska prasa kobieca w latach 1820–1918, (Warszawa: Wydawnictwo SBP, 1999).Gajkowska, Cecylia. „Malecka z Fryzów Wanda (1800–1860)”, Polski Słownik Biograficzny, t. 19, (Wrocław: Zakład Narodowy im. Ossolińskich, 1974).Giller, Agaton. Opisanie zabajkalskiej krainy w Syberii, (Lipsk: F.A. Brockhaus, 1867).Jędrzejewski, Tomasz. Literatura w warszawskiej prasie kulturalnej pogranicza oświecenia i romantyzmu, (Kraków: Universitas, 2016).Kowalska, Aniela. Warszawa literacka w okresie przełomu kulturalnego, (Warszawa: Państwowy Instytut Wydawniczy, 1961).Maślanka, Julian. Literatura a dzieje bajeczne (Warszawa: Państwowe Wydawnictwo Naukowe, 1990).Mortkowiczówna, Hanna. Podanie o Wandzie. Dzieje wątku literackiego (Warszawa: W Księgarni Towarzystwa Wydawniczego, 1927).The poems of Ossian, the son of Fingal. Translated by James Macpherson, Esq, t. 2, (Edinburgh: A. Constable and Co., 1805).Pol, Wincenty. Pamiętnik do literatury polskiej XIX wieku w dwudziestu prelekcjach mianych w radnej sali miasta Lwowa, (Lwów: nakładem autora, 1866).Pusz, Wiesław. „Nowy Parnas” przedromantycznej Warszawy. Bruno Kiciński i grono jego współpracowników, (Wrocław: Zakład Narodowy im. Ossolińskich, 1979).Rozmysł, Michał. „Mit założycielski Krakowa w twórczości autorek dziewiętnastowiecznych (Łubieńska – Malecka – Bośniacka – Deotyma)”, w: Sabina Brzozowska, Marek Dybizbański (red.),
Mity założycielskie w literaturze XIX wieku (Opole: Wydawnictwo Uniwersytetu Opolskiego, 2018), 33–44.Sinko, Zofia. Proza fabularna w czasopismach polskich 1801–1830, (Wrocław: Zakład Narodowy im. Ossolińskich, 1988).Sokół, Zofia. „Z badań nad polską prasą kobiecą w latach 1818–1939”, Kwartalnik Historii Prasy Polskiej, t. 22, 1983, 5–12.Sroczyńska, Bogumiła. „Działalność publicystyczna i literacka pierwszej polskiej kobiety redaktorki, Wandy Maleckiej (1800–1860)”, Kwartalnik Historii Prasy Polskiej, t. 24, 1985–1986, 5–26.Żyrek-Horodyska, Edyta. „Gazeciarki. O dziennikarskich karierach kobiet w I połowie XIX wieku”, Rocznik Historii Prasy Polskiej, t. 22, z. 3, 2019,19–40.1(14)537
Real-Time Monitoring of Apoptosis by Caspase-3-Like Protease Induced FRET Reduction Triggered by Amyloid Aggregation
No full textAmyloid formation is cytotoxic and can activate the caspase cascade. Here, we monitor caspase-3-like activity as reduction of fluorescence resonance energy transfer (FRET) using the contstruct pFRET2-DEVD containing enhanced cyan fluorescent protin (EYFP) linked by the caspase-3 specific cleavage site residues DEVD. Beta-TC-6 cells were transfected, and the fluoorescence was measured at 440 nm excitation and 535 nm (EYFP) and 480 nm (ECFP) emission wavelength. Cells were incubated with recombinant pro lset Amyloid Polypeptide (rec prolAPP) or the processing metabolites of prolAPP; the N-terminal flanking peptide withIAPP (recN+IAPP); IAPP with the C-terminal flanking peptied (recIAPP+C) and lslet Amyloid Polypeptide (recIAPP). Peptides were added in solubilized from (50 mu M) or as performed amyloid-like fibrils, or as a combination of these. FRET was measured and incubation with a mixture of solubilized peptide and performed fibrils resulted in loss of FRET and apoptosis was determined to occurein cells incubated with recproIAPP (49%), recN+IAPP (46%), recIAPP (72%) and recIAPP+C (59%). These results show that proIAPP and the processing intermediates reside the same cell toxic capacity as IAPP, and they can all have a central role in the reduction of beta-cell number in type 2 diabetes.Original Publication:Johan F Paulsson, Sebastian Schultz, Martin Kohler, Ingo Leibiger, Per-Olof Berggren and Gunilla Westermark, Real-Time Monitoring of Apoptosis by Caspase-3-Like Protease Induced FRET Reduction Triggered by Amyloid Aggregation, 2008, EXPERIMENTAL DIABETES RESEARCH, (2008), 865850.http://dx.doi.org/10.1155/2008/865850Copyright: Author
Fibronectin and basement membrane components in renal amyloid deposits in patients with primary and secondary amyloidosis
No full textSUMMARY
Kidney biopsies from one patieni with primary (AL) and three with secondary (AA) amyloidosis were used for an ultrastructural study of the collocalization of basement membrane proteins and the extracellular matrix protein fibronectin within amyloid deposits. Antibodies against amyloid P component, laminin. and heparan sulphate proteoglycan core protein all reacted with the basement membranes and the amyloid depositions in AA and AL amyloidosis. Monoclonal and polyclonal antibodies against collagen type IV reacted only with the basement membranes. Anti-fibronectin reaction was found in association with the basement membranes in all four cases, while labelling of amyloid depositions was found only in one of the AA amyloid cases and in the AL amyloid depositions. It is concluded that basement membrane components may be of importance for the formation of amyloid fibrils.</jats:p
Massive Vascular AA‐Amyloidosis: A Histologically and Biochemically Distinctive Subtype of Reactive Systemic Amyloidosis
No full text
Real and Nominal Wage Adjustment in Open Economies
No full textHow are wages set in an open economy? What role is played by demand pressure, international competition, and structural factors in the labour market? How important is nominal wage rigidity and exchange rate policy for the evolution of real wages and competitiveness? To answer these questions, we formulate a theoretical model of wage bargaining in an open economy and use it to derive a simple wage equation where all parameters have clear economic interpretations. We estimate the wage equation on data for aggregate manufacturing wages in Denmark, Finland, Norway, and Sweden from the mid 1960s to the mid 1990s.wage formation, efficiency wage, turnover, bargaining, rent sharing, nominal wage rigidity, exchange rate policy, competitiveness
Alkali-Degradation of Amyloid: An Ancient Method Useful for Making Monoclonal Antibodies Against Amyloid Fibril Proteins
No full textThe systemic amyloidoses constitute a group of life-threatening disorders at which one out of about 15 different proteins have polymerized into fibrils. Prognosis and treatment varies widely and depends on the biochemical type. Determination of this has usually to be performed by immunohistochemistry which is a challenge because of lack of monospecific antibodies that can be used on formaldehyde-fixed tissue sections. We have here used an old method to create immunogenic fragments of AL-amyloid fibrils by partial degradation and solubilization with sodium hydroxide. The mouse monoclonal antibody pwlam raised against this material, labelled AL-amyloid deposits of lambda origin strongly and specifically in sections of formaldehyde-fixed and paraffin-embedded tissues.</p
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