675 research outputs found
Induction of pulmonary HLA-G expression by SARS-CoV-2 infection
No full textThe non-classical human leukocyte antigen (HLA)-G exerts immune-suppressive properties modulating both NK and T cell responses. While it is physiologically expressed at the maternal–fetal interface and in immune-privileged organs, HLA-G expression is found in tumors and in virus-infected cells. So far, there exists little information about the role of HLA-G and its interplay with immune cells in biopsies, surgical specimen or autopsy tissues of lung, kidney and/or heart muscle from SARS-CoV-2-infected patients compared to control tissues. Heterogeneous, but higher HLA-G protein expression levels were detected in lung alveolar epithelial cells of SARS-CoV-2-infected patients compared to lung epithelial cells from influenza-infected patients, but not in other organs or lung epithelia from non-viral-infected patients, which was not accompanied by high levels of SARS-CoV-2 nucleocapsid antigen and spike protein, but inversely correlated to the HLA-G-specific miRNA expression. High HLA-G expression levels not only in SARS-CoV-2-, but also in influenza-infected lung tissues were associated with a high frequency of tissue-infiltrating immune cells, but low numbers of CD8(+) cells and an altered expression of hyperactivation and exhaustion markers in the lung epithelia combined with changes in the spatial distribution of macrophages and T cells. Thus, our data provide evidence for an involvement of HLA-G and HLA-G-specific miRNAs in immune escape and as suitable therapeutic targets for the treatment of SARS-CoV-2 infections
Sleep spindle maturity promotes slow oscillation-spindle coupling across child and adolescent development
No full textThis project provides code and data to reproduce the results, figures, and supplementary material of the paper: Joechner, A. K., Hahn, M. A., Gruber, G., Hoedlmoser, K., & Werkle-Bergner, M. (2023). Sleep spindle maturity promotes slow oscillation-spindle coupling
across child and adolescent development. eLIFE DOI: https://doi.org/10.7554/eLife.8356
Sleep spindle maturity promotes slow oscillation-spindle coupling across child and adolescent development
No full textThis project provides code and data to reproduce the results, figures, and supplementary material of the paper: Joechner, A. K., Hahn, M. A., Gruber, G., Hoedlmoser, K., & Werkle-Bergner, M. (2023). Sleep spindle maturity promotes slow oscillation-spindle coupling
across child and adolescent development. eLIFE DOI: https://doi.org/10.7554/eLife.8356
Sleep spindle maturity promotes slow oscillation-spindle coupling across child and adolescent development
No full textThis project provides code and data to reproduce the results, figures, and supplementary material of the paper: Joechner, A. K., Hahn, M. A., Gruber, G., Hoedlmoser, K., & Werkle-Bergner, M. (2023). Sleep spindle maturity promotes slow oscillation-spindle coupling
across child and adolescent development. eLIFE DOI: https://doi.org/10.7554/eLife.8356
Leitfadenorientierte Interviews - eine geeignete Methode zur Ergründung der Handlungsrelevanz von Therapiestandards in der kardiologischen Versorgung?
No full textSiering U, Staender J, Bergner E. Leitfadenorientierte Interviews - eine geeignete Methode zur Ergründung der Handlungsrelevanz von Therapiestandards in der kardiologischen Versorgung? In: Schaeffer D, Müller-Mundt G, eds. Qualitative Gesundheits- und Pflegeforschung. 2002: 285-304
G-Proteine und Melanomzellen
No full textMit dieser Arbeit konnten die Pertussistoxin-sensitiven heterotrimeren G-Protein- Subtypen Galphai2 und Galphai3 sowie das Pertussistoxin-insensitive Galpha16 in Melanomzellen auf RNA- und auf Proteinebene nachgewiesen werden. Die Galphai-Proteine wurden durch Pertussistoxin ADP-ribosyliert. Mit Hilfe des MTT-Tests konnte dargestellt werden, dass dadurch die Proliferation der Melanomzellen unterdrückt wurde. Zum anderen konnten auch die kleinen, monomeren G-Proteine der Rho-Familie (RhoA, RhoB, RhoG, Rac1 und Cdc42) in Melanomzellen auf Proteinebene nachgewiesen werden. Durch Clostridium difficile Toxin B wurden diese G-Proteine der Rho-Familie modifiziert. Diese Modifizierungen bewirkten bei den Melanomzellen eine Abnahme der Proliferation, die mit dem Bromodesoxyuridineinbau-Test nachgewiesen wurde
Pseudoscalar-pole contributions to the muon at the physical point
No full textPseudoscalar-pole diagrams are an important component of estimates of the
hadronic light-by-light (HLbL) contribution to the muon g−2. We report on our computation of the transition form factors P→γ∗γ∗ for the neutral pseudoscalar mesons P=π0
and η . The calculation is performed using twisted-mass lattice QCD with physical quark masses. On the lattice, we have access to a broad range of (space-like) photon four-momenta and therefore produce form factor data complementary to the experimentally accessible single-virtual direction, which directly leads to an estimate of the pion- and η
-pole components of the muon g−2 . For the pion, our result for the g−2 contribution in the continuum is comparable with previous lattice and data-driven determinations, with combined relative uncertainties below 10% . For the η meson, we report on a preliminary determination from a single lattice spacing
Identifizierung und Charakterisierung HLA-G-regulierender microRNAs
No full textDas humane Leukozyten-Antigen G (HLA-G) ist ein nicht-klassisches HLA Klasse I-Molekül. Es wirkt immunmodulierend und vermittelt immunologische Toleranz. Bei Tumorerkrankungen, wie dem Nierenzellkarzinom (NZK), kann die Expression von HLA-G zur Immunevasion beitragen. In dieser Dissertation wird die klinische Relevanz der Expression von HLA-G und HLA-G-regulierender microRNAs (miRs) in NZK-Zelllinien und -Tumoren charakterisiert. Des Weiteren werden bisher unbeschriebene HLA-G-regulierende miRs identifiziert und validiert. Unter Anwendung 2D-Gel-basierender vergleichender Proteomanalysen kann mit 14-3-3β ein neues miR-152-Zielgen beschrieben werden. Die tumor-suppressive miR-152 nimmt eine duale Funktion ein, indem sie HLA-G und 14-3-3β reguliert. Daher eignet sich die miR-152 als mögliches anti-tumorales Agens analog zu den bereits verwendeten miR-34a mimics.The human leukocyte antigen G (HLA-G) is a non-classical HLA class I molecule. It reacts immune modulatory and mediates immunological tolerance. In tumor diseases like renal cell carcinoma (RCC) the expression of HLA-G contributes to the immune evasion of the tumor. In this dissertation the clinical relevance of the expression of HLA-G and HLA-G-regulatory microRNAs (miRs) in RCC cell lines and tumors is characterized. Furthermore, unknown novel HLA-G regulatory miRs are identified and validated. By application of 2D gel based comparative proteome analyses 14-3-3β a novel miR-152-target gene is identified. The tumor suppressive miR-152 exerts a dual function by regulation of HLA-G and 14-3-3β. Thus, the miR-152 fits as possible anti-tumoral agent analogous to the already used miR-34a mimics.von Simon Jasinski-Bergne
Quark masses and decay constants in Nf=2+1+1 isoQCD with Wilson clover twisted mass fermions
No full textWe present a preliminary study of the pion, kaon and D-meson masses and decay constants in isosymmetric QCD, as well as a preliminary result for the light-quark renormalized mass. The analysis is based on the gauge ensembles produced by ETMC with N f = 2 + 1 + 1 flavours of Wilson-clover twisted mass quarks, spanning a range of lattice spacings from ∼ 0.10 to 0.07 fm and include configurations at the physical pion point on lattices with linear size up to L ∼ 5.6 fm
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