735 research outputs found
Heme-oxygenase-1 Gene Expression Ameliorates Cardiac Dysfunction Following Myocardial Infarction In C57 Mice By Recruitment Of Angiogenic Factors And Angiogenesis In C57 Mice
No full textDavid Sacerdoti1; Sumit R Monu2; Paola Pesce1; Stephen J Peterson3; Komal Sodhi2; Nader G Abraham
FIGURE 5. Lactarius drassinus. a–b in Two new species of genus Lactarius (Russulaceae) from North-western Himalaya, India
No full textFIGURE 5. Lactarius drassinus. a–b. Basidiomata in the field; c. Latex; d. Cheiloleptocystidia; e. Cheilomacrocystidia; f. Basidia; g. Pseudocystidia; h. Pleuromacrocystidia; i. Transverse section through pileipellis; j. Basidiospores under light microscope; k,l. Basidiospores under SEM. Scale bars: a–c=20 mm, d–j = 10 μm. k=2 μm, l=1 μm. Photos a–c by: Y. P Sharma and d–l by: Komal Verma.Published as part of Verma, Komal, Mehmood, Tahir, Uniyal, Priyanka, Kapoor, Rupam & Sharma, Yash Pal, 2021, Two new species of genus Lactarius (Russulaceae) from North-western Himalaya, India, pp. 253-265 in Phytotaxa 500 (4) on page 261, DOI: 10.11646/phytotaxa.500.4.1, http://zenodo.org/record/542466
FIGURE 3. Lactarius indoevosmus. a–e in Lactarius indoevosmus and L. kanadii (Russulaceae), two new species from the northwestern Himalayas, India, inferred from morphology and molecular data
No full textFIGURE 3. Lactarius indoevosmus. a–e. Basidiomes in the field; f. Basidia; g. Cheilocystidia; h. Pleurocystidia; i. Pseudocystidia; j. Paracystidia; k,l. Transverse section through pileipellis; m. Basidiospores under light microscope; n–p. Basidiospores under SEM. Scale bars: a–c = 20 mm, f–m = 10 μm. n,o = 2 μm, p = 5 μm. Photos a–e by Y. P Sharma and f–l by Komal Verma.Published as part of Verma, Komal, Mehmood, Tahir, Uniyal, Priyanka & Sharma, Yash Pal, 2022, Lactarius indoevosmus and L. kanadii (Russulaceae), two new species from the northwestern Himalayas, India, inferred from morphology and molecular data, pp. 165-177 in Phytotaxa 541 (2) on page 170, DOI: 10.11646/phytotaxa.541.2.6, http://zenodo.org/record/638874
Studies on in vitro bioavailability and starch hydrolysis in zinc fortified ready-to-eat parboiled rice (komal chawal)
No full textZinc fortified parboiled rice (komal chawal) was produced from a low amylose variety of rice by applying 'brown rice parboiling' method. In addition to the effect of milling on fortification, the effectiveness of fortification upon the amount of bioaccessible (in vitro digest) and bioavailable (cellular uptake) form of Zn was tested. The effect on glycaemic index was also assessed by employing an in vitro starch hydrolysis assay. The bioaccessible form of Zn in the unmilled fortified rice were ranged in between 4.24 and 11.07 mg/100 g, which was significantly higher (p < 0.05) than the milled and unfortified parboiled rice. Similarly, the % absorption of bioavailable Zn was negligible in the unfortified parboiled rice as compared to the fortified rice (14.5-24.5%). The estimated GI of fortified parboiled rice samples was in the range of 50.97-59.79, which was lower than the unfortified parboiled rice (58.80-62.53) and raw rice (78.71-84.64). The results thus demonstrated that Zn fortified komal chawal can be a novel and rapidly produced micronutrient enhanced ready-to-eat rice.</p
Access and use of the Internet in the libraries of Lahore, Pakistan
Get PDFThis study is to find out the level of the Internet access in libraries, its uses by library staff and the problems regarding access and use of Internet in libraries of Lahore, Pakistan. The research is based on primary data. A structured questionnaire survey was conducted by (n=75) 48 academic libraries (university and college), 21 special libraries, five public libraries and one school library in the city of Lahore. Findings of the study may be useful for policy makers to understand the status of the Internet access and its need as well as for library schools to revise the curriculum to improve the Internet skills of future librarians
Geobacter sulfurreducens inner membrane cytochrome transcriptional and phenotypic data
No full textThe data files include raw data as well as analyzed results for transcriptional analysis of WT G. sulfurreducens and mutant lacking BccR (GSU0598) under fumarate vs iron citrate growth conditions. This dataset also includes the phenotypic data files for experiments associated with this project.Geobacter sulfurreducens utilizes extracellular electron acceptors such as Mn(IV), Fe(III), syntrophic partners, and electrodes that vary from +0.4 to −0.3 V vs. Standard Hydrogen Electrode (SHE), representing a potential energy span that should require a highly branched electron transfer chain. Here we describe CbcBA, a bc-type cytochrome essential near the thermodynamic limit of respiration when acetate is the electron donor. Mutants lacking cbcBA ceased Fe(III) reduction at −0.21 V vs. SHE, could not transfer electrons to electrodes between −0.21 and −0.28 V, and could not reduce the final 10% – 35% of Fe(III) minerals. As redox potential decreased during Fe(III) reduction, cbcBA was induced with the aid of the regulator BccR to become one of the most highly expressed genes in G. sulfurreducens. Growth yield (CFU/mM Fe(II)) was 112% of WT in ∆cbcBA, and deletion of cbcL (an unrelated bc-cytochrome essential near −0.15 V) in ΔcbcBA increased yield to 220%. Together with ImcH, which is required at high redox potentials, CbcBA represents a third cytoplasmic membrane oxidoreductase in G. sulfurreducens. This expanding list shows how metal-reducing bacteria may constantly sense redox potential to adjust growth efficiency in changing environments.Office of Naval Research: N00014-16-1-2194, and N00014-18-1-2632.Joshi, Komal; Chan, Chi Ho; Bond, Daniel R. (2021). Geobacter sulfurreducens inner membrane cytochrome transcriptional and phenotypic data. Retrieved from the University Digital Conservancy, https://doi.org/10.13020/5AMD-ZW33
Heme-oxygenase-1 Induction Improves Type-1 Cardiorenal Syndrome Only In Mice With Impaired AngII-induced Lymphocyte Activation (SCID Mice)
No full textRational: The absence of lymphocyte activity protects SCID mice from AngII-induced hypertension facilitating blood pressure-induced sodium excretion, possibly via the stimulation of eNOS- and COX-2-dependent pathways. Type-1 cardiorenal syndrome (CRS-1), characterized by acute kidney dysfunction secondary to deterioration in cardiac function, is caused by renal arteriolar vasoconstriction, mediated by the activation of renin-angiotensin and sympathetic nervous systems (RAAS, SNS). Heme Oxygenase-1 (HO-1) induction improves renal function, but not renal vasoconstriction, in AngII-induced hypertension, and causes the desensitization of vascular smooth muscle to phenylephrine.
Objectives: We evaluated whether AngII resistant SCID mice develop CRS-1 as occurs in control mice, and the differential effects of HO1 induction on renal hemodynamics in CRS-1.
Methods: Post ischemic heart failure was induced in C57 and SCID mice by left anterior coronary artery ligation. Mice were divided in 4 groups: sham, myocardial infarction (MI), MI treated with cobalt protoporphyrin (CoPP), an inducer of HO-1, in the presence and absence of HO activity inhibitor, stannous mesoporphyrin (SnMP). All mice underwent echocardiography (ejection fraction, EF) and renal echoDoppler (arterial pulsatility index, K-PI) examination 30 days after surgery.
Results: EF was significantly reduced both in control and SCID mice after MI (C57: sham 0.60±0.07, MI 0.45±0.04, p<0.05; SCID: sham 0.60±0.06, MI: 0.46±0.1, p<0.01). K-PI was significantly increased in MI groups compared to sham groups (C57: sham 0.98±0.05, MI 1.12±0.11, p<0.05; SCID: sham 0.72±0.08, MI 1.37±0.37, p<0.05). HO1 induction improved renal vasoconstriction only in SCID mice (SCID K-PI: MI+CoPP 0.9±0.19 p<0.5çC57 K-PI: MI+CoPP 1.05±0.15, n.s.). In SCID mice SnMP reversed the effect of CoPP on renal vasoconstriction.
Conclusion: CRS-1 is similar in SCID and control mice and is associated with increased renal arterial resistance. HO-1 induction improves renal vasoconstriction only in SCID (AngII resistant) mice with CRS-1, suggesting that increased HO-1 activity cannot protect the kidney from AngII-induced lymphocyte activation, but only from SNS-induced vasoconstriction.
Author Disclosures: P. Pesce: None. D. Sacerdoti: None. S.R. Monu: None. K. Sodhi: None. M. Boldrin: None. N.G. Abraham: None.
Key Words: Angiotensin II • Renal circulation • Heart failur
Enhanced production of a natural blue pigment indigoidine as sustainable fabric dyes using metabolically engineered Corynebacterium glutamicum
No full textCurrent industrial production of dyes for textiles involves the use of many toxic chemicals, which has caused severe water pollution. For this, indigoidine has gained attention as an alternative natural blue dye that would contribute to the sustainable textile industry. However, bio-based production of indigoidine needs to be further improved to compete with existing synthetic blue dyes. Here, we report a metabolically engineered Corynebacterium glutamicum that can produce indigoidine at high concentration and productivity. First, blue-pigment indigoidine synthetase (bpsA) gene from Streptomyces lavendulae was expressed in C. glutamicum, which carries strong fluxes toward L-glutamate, a precursor of indigoidine. Production performance of this base strain, already producing 7.3 ± 0.3 g/L indigoidine from flasks, was further enhanced by streamlining intracellular supply of the precursors L-glutamate and L-glutamine, strengthening phosphotransferase system-independent glucose uptake system, channeling carbon fluxes from glycolysis to tricarboxylic acid (TCA) cycle, and minimizing byproducts formation. The final strain named BIRU11 produced 49.30 g/L indigoidine with a productivity of 0.96 g/L/h from fed-batch fermentation, the highest titer and productivity to date. Finally, indigoidine from the fed-batch fermentation of the BIRU11 strain was utilized to dye white cotton fabrics to examine its color and performance. Our study demonstrates the potential sustainable production of fabric dyes by using a metabolically engineered bacterium
The Relationship between Wastewater Toxic Substances and Alzheimer’s disease
No full textabstract: Alzheimer’s disease (AD) is a neurodegenerative disease resulting in loss of cognitive function and is not considered part of the typical aging process. Recently, research is being conducted to study environmental effects on AD because the exact molecular mechanisms behind AD are not known. The associations between various toxins and AD have been mixed and unclear. In order to better understand the role of the environment and toxic substances on AD, we conducted a literature review and geospatial analysis of environmental, specifically wastewater, contaminants that have biological plausibility for increasing risk of development or exacerbation of AD. This literature review assisted us in selecting 10 wastewater toxic substances that displayed a mixed or one-sided relationship with the symptoms or prevalence of Alzheimer’s for our data analysis. We utilized data of toxic substances in wastewater treatment plants and compared them to the crude rate of AD in the different Census regions of the United States to test for possible linear relationships. Using data from the Targeted National Sewage Sludge Survey (TNSSS) and the Centers for Disease Control and Prevention (CDC), we developed an application using R Shiny to allow users to interactively visualize both datasets as choropleths of the United States and understand the importance of this area of research. Pearson’s correlation coefficient was calculated resulting in arsenic and cadmium displaying positive linear correlations with AD. Other analytes from this statistical analysis demonstrated mixed correlations with AD. This application and data analysis serve as a model in the methodology for further geospatial analysis on AD. Further data analysis and visualization at a lower level in terms of scope is necessary for more accurate and reliable evidence of a causal relationship between the wastewater substance analytes and AD.
GitHub Repository: https://github.com/komal-agrawal/AD_GIS.gi
Viscoelasticity Analysis of Coarse-grained Cytoskeletal Simulations with Cytosim and Cytocalc
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