2,160 research outputs found
Differential stability of 2'F-ANA*RNA and ANA*RNA hybrid duplexes: roles of structure, pseudohydrogen bonding, hydration, ion uptake and flexibility
No full text14 pags., 7 figs., 3 tabs.Hybrids of RNA with arabinonucleic acids 2′F-ANA and ANA have very similar structures but strikingly different thermal stabilities. We now present a thorough study combining NMR and other biophysical methods together with state-of-the-art theoretical calculations on a fully modified 10-mer hybrid duplex. Comparison between the solution structure of 2′F-ANA•RNA and ANA•RNA hybrids indicates that the increased binding affinity of 2′F-ANA is related to several subtle differences, most importantly a favorable pseudohydrogen bond (2′F-purine H8) which contrasts with unfavorable 2′-OH-nucleobase steric interactions in the case of ANA. While both 2′F-ANA and ANA strands maintained conformations in the southern/eastern sugar pucker range, the 2′F-ANA strand's structure was more compatible with the A-like structure of a hybrid duplex. No dramatic differences are found in terms of relative hydration for the two hybrids, but the ANA•RNA duplex showed lower uptake of counterions than its 2′F-ANA•RNA counterpart. Finally, while the two hybrid duplexes are of similar rigidities, 2′F-ANA single strands may be more suitably preorganized for duplex formation. Thus the dramatically increased stability of 2′F-ANA•RNA and ANA•RNA duplexes is caused by differencesin at least four areas, of which structure and pseudohydrogen bonding are the most important. © The Author(s) 2010. Published by Oxford University Press.Spanish Ministerio de Ciencia e Innovacion (grants
CTQ2007-68014-C02-02 to CG and BIO2009-10964 to
MO); Fundacion Marcelino Botin (grant to MO);
Canadian Institutes for Health Research (grant to
M.J.D.); Natural Sciences and Engineering Research
Council of Canada (postgraduate scholarship to
J.K.W.). Funding for open access charge: Canadian
Institutes for Health Research
Viajantes ex-cêntricas nas histórias de Ana Miranda
No full textTese (doutorado) - Universidade Federal de Santa Catarina, Centro de Comunicação e Expressão. Programa de Pós-Graduação em Literatura.No conjunto das narrativas ficcionais da escritora brasileira Ana Miranda, a temática da viagem # considerada uma das mais férteis da literatura ocidental de todos os tempos # ocupa um espaço de centralidade, podendo até mesmo ser vista como o mais importante eixo de estruturação de suas obras. O estudo realizado pela presente tese tem por objetivo analisar o tratamento dado ao tema da viagem nos romances Desmundo, O retrato do rei, Dias & Dias e Amrik, evidenciando que por intermédio das narradora-viajantes # Oribela, Mariana, Feliciana e Amina # a autora promove um diálogo entre diferentes culturas, gêneros, etnias e gerações, ao mesmo tempo em que estabelece um profícuo diálogo com o passado em sua invariante problematização concernente aos limites e cruzamentos entre o discurso ficcional e os discursos narrativos extraliterários que o cercam, sobretudo o histórico e o biográfico. In the whole of Brazilian writer Ana Miranda#s fictional narrative, the travel thematic # considered one of the most fertile themes of the western literature of all times # occupies a central space, and can even been seen as the most important structuring axis of Miranda#s works. The aim of the present thesis is to analyze the treatment given to the travel subject in the novels Desmundo, O retrato do rei, Dias & Dias and Amrik, emphasizing that, through the traveling narrators, Oribela, Mariana, Feliciana and Amina, the author promotes a connection between different cultures, genders, ethnics and generations, at the same time that establishes a profitable dialogue with the past in her invariant problematization concerning the limits and crossings between the fictional and the extra-literary narrative discourses that surrounds it, mainly the historical and the biographic ones
Book review: R. Saltman, R. Busse and E. Mossialos (eds.), Regulating entrepreneurial behaviour in European health care systems. Open Universtiy Press, 2002
No full text(Ana Marta
Human trafficking: a comparative analysis of why countries with similar characteristics have different situations
No full textNumerous factors which promote human trafficking have been examined by many scholars, yet little attention has been placed on the role of adolescent single mothers as a significant factor that increases the vulnerability of these young women to trafficking. This study aims to understand and explain why two countries with similar characteristics in geography, political and socio-economic systems present a very different picture in their sex trafficking patterns and challenges. This is a case study that compares Argentina and Brazil along a variety of indicators relevant to human trafficking. Although similar with respect to several characteristics analyzed in their incidence of sex trafficking, Argentina and Brazil do differ in one major factor that on its face could account for the differences in their sex trafficking practices --birth rate among adolescent girls aged 15 to 19. This rate is higher in Brazil than that in Argentina. This study seeks to answer the following questions: 1. - Does this difference account for the difference in sex trafficking practices and if so, how? 2. - Is this difference explained by something else (e.g. other than birth rate)? 3. - What other factors might contribute to the difference in sex trafficking practices? Two main sources of information were used in this comparative analysis: (1) data collected from reports, databases from intergovernmental organizations, governmental agencies, NGOs, and academic literature; and (2) court sentences from both countries, to both flesh out the factors identified, and more importantly, to support or refute the assumption with respect to the role of adolescent birth rates. Both qualitative and quantitative analyses were employed. This study reveals four main differences between Argentina and Brazil that collectively, indeed, shed light on their sex trafficking differences: 1) methods of recruitment, 2) “modus operandi” and government complicity, 3) gender inequality and 4) the incidence of teen single motherhood as a vulnerability factor to trafficking. Findings in this study suggest that matrifocality, the woman-centered family, is a key component in understanding how and why Brazilian adolescent single mothers are an easy target for traffickers.Ph. D.Includes bibliographical referencesIncludes vitaby Ana R. Sverdlic
The aesthetics of the labyrinth: baroque and modernity in Ana Hartherly
No full textA estética do labirinto: barroco e modernidade em Ana Hatherly tem como objetivo estudar a criação poética da autora portuguesa, apontando suas afinidades estéticas com as tradições barroca e maneirista, e em especial com os labirintos (ou textos visuais) do século XVII. Ana Hatherly, que integrou o movimento da Poesia Experimental Portuguesa (PO-EX), na década de 1960, fez uma releitura criativa dessa herança cultural, ao mesmo tempo em que dialogou com formas e procedimentos da vanguarda internacional (e em particular com a Poesia Concreta), visando a reinvenção da escrita, mesclando recursos da literatura, da música, da pintura e outras formas de expressão. A escrita renovada de Ana Hatherly demanda um outro tipo de recepção por parte do leitor, que participa da descoberta ou da construção de significados, numa reinvenção da leitura. A estratégia criativa de Ana Hatherly aproxima-se, portanto, do conceito de obra aberta, formulado por Umberto Eco e Haroldo de Campos, que pode ser definida como um tipo de estrutura estética que permite múltiplas leituras, a partir de certo conjunto de regras estabelecidas pelo autor (que revela assim o aspecto de jogo da obra de arte). Este é um dos conceitos centrais discutidos na dissertação, aplicado à análise de obras da autora como as Tisanas, com apoio adicional nas formulações teóricas de autores como Affonso Ávila, Gustav R. Hocke e Johann Huizinga. Por fim, discutiremos os conceitos de vanguarda com base em autores como Marjorie Perloff, Renato Poggioli e Richard Kostelanetz, com o objetivo de apontar a atualidade de muitas das propostas do movimento da PO-EX, que não se limitam a um determinado contexto histórico, relacionando-se, inclusive, com experiências realizadas nos dias de hoje, no campo da poesia eletrônica ou digital.The aesthetics of the labyrinth: baroque and modernity in Ana Hartherly aims to study the poetic creation of this Portuguese author pointing out its aesthetics choices in relation to the baroque and the mannerism, particularly the labyrinths (or visual texts) produced in the 17th century. Ana Hatherly, who took part in the Portuguese Experimental Poetry movement (PO-EX), in the 1960\'s, performed a creative rereading of this cultural heritage, and at the same time established a link with forms and procedures of the international vanguard (especially with the Concrete Poetry), pursuing the reinvention of the writing, merging resources of literature, music, painting and other forms of expression. Ana Hatherly\'s renewed writing demands another way of approaching, one that leads the reader into discoveries or constructions of meanings, in a reinvention of the act of reading. Ana Hatherly\'s creative strategy therefor is closely related with the concept of an open piece of work, formulated by Umberto Eco and Haroldo de Campos, and means a type of aesthetic structure that allows multiple interpretations, once a certain set of rules is established by the author (rendering, this way, a gamelike work). That is one of the key concepts to be discussed in this dissertation, where her works, such as Tisanas will be analyzed with additional theoretical support of works by Affonso Ávila, Gustav R. Hocke, and Johann Huizinga. Finally, we shall discuss the concepts of vanguard with theoretical support of Marjorie Perloff, Renato Poggioli and Richard Kostelanetz, aiming to point out how contemporary many of the PO-EX propositions are. Those are not bonded to a determined historical context, and even nowadays can be observed in experiences in the field of the electronic or digital poetry
Determining the need for an expanded fitness component in the Fire Science Program at Milwaukee Area Technical College
No full textIncludes bibliographical references
Does strict employment protection discourage job creation? Evidence from Croatia
No full textEmployment protection legislation in Croatia is among the most strict in Europe. Firing is difficult and costly, and flexible forms of employment are limited. Is this apparent rigidity reflected-as one would expect based on standard economic theory-in low labor market dynamics? Is job creation low and hiring limited? Is the job security of insiders achieved at the cost of outsiders not being able to enter thelabor market? The author attempts to answer these questions by examining job flows. If the employment protection legislation is binding, then job and worker turnover should be low. He shows that this is indeed the case. Hiring is limited and the average job tenure is very long in Croatia. Job destruction is low, however job creation is still lower. The result is accumulation of unemployment, in large part due to new labor market entrants not being able to find a job. The high degree of job protection also seems to strengthen the bargaining position of insiders and results in relatively high wages. So, wages in Croatia are higher than among its competitors, even after adjusting for productivity. These high labor costs are likely to contribute to limited job creation in existing firms, but also are likely to discourage the entry of-and thus job creation in-new firms. The author presents evidence that firm growth has been indeed limited in Croatia, contributing to the low employment level. The author examines other potential causes of high unemployment in Croatia (the unemployment benefit system, labor taxation, the wage structure, and skill and spatial mismatches). He argues that they do not play a substantial part in accounting for poor labor market outcomes in Croatia. The author concludes that the stringent employment protection legislation is the key labor market institution behind low job creation and high unemployment. Based on this he recommends specific measures aimed at liberalizing the labor market to foster job creation and employment.Labor Management and Relations,Labor Policies,Labor Markets,Environmental Economics&Policies,Trade Finance and Investment,Labor Markets,Labor Management and Relations,Labor Standards,Banks&Banking Reform,Environmental Economics&Policies
On the sensitivity of Von Neuman and Morgenstern abstract stable sets : the stable and the individual stable bargaining set
No full textAnateismo e Certezze negative
No full textThe author focuse on the temporal dimension of "ana" which is thematized by R. Kearney's position in order to stress on advantageous trait of negative is comparated with Jean-Luc Marion's reflection on "negative certidudes". Such a reflexion releases definitively the negative from the oppositional residue which instead is still present in Kearney's position
Produção/reprodução e transformação do comportamento alcoolista nos contextos culturais da família e do trabalho /
No full textDissertação (Mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências da Saúde.A presente dissertação de mestrado trata de uma investigação realizada a partir dos resultados obtidos na Prática Assistencial, quando foi oferecida, a três famílias de servidores alcoolistas, uma modalidade de assistência de enfermagem pautada na Teoria Sistêmica de Família e na Teoria de Leininger. Buscando ampliar e complementar os dados obtidos, foi elaborado um projeto que deu origem a este estudo, o qual tem por objetivos: analisar as influências do contexto do trabalho na produção/reprodução e transformação do comportamento alcoolista; analisar os nexos entre a produção/reprodução e transformação do comportamento alcoolista no contexto do trabalho e da família e propor alternativas para a transformação do comportamento alcoolista no trabalho, mediante as potencialidades identificadas na família e no trabalho. Este é um estudo exploratório, qualitativo, realizado com três funcionários alcoolistas da UFSM (Universidade Federal de Santa Maria) vinculados às famílias anteriormente pesquisadas. A metodologia constitui-se na análise de fichas funcionais, observação participante no ambiente de trabalho e entrevista com chefias, quando necessário. Após a etapa de coleta de dados, foi possível analisar os nexos entre o comportamento alcoolista na família e o comportamento alcoolista no trabalho. Identificar a produção/reprodução do comportamento alcoolista no contexto do trabalho viabilizou a sinalização de uma proposta de transformação, para a qual aponto indicadores de mudanças; e também reconheço a necessidade de espaços para desenvolver um trabalho integrado entre os serviços que tratam o alcoolismo, principalmente ao nível institucional, no qual me incluo, na tentativa de buscar outras maneiras de investir em mudanças gradativas do comportamento alcoolista
- …
