1,721,338 research outputs found
Research dataset for the fuzzy measurement of precarious employment in Brazil
No full textThis research dataset operationalizes the measurement of precarious employment in Brazil using a fuzzy set methodology. The data structure accounts for a complex survey design, incorporating variables for Primary Sampling Units (upa), strata (stratum), and individual/household weights (weight) to ensure statistical validity. Its core analytical variables are membership grades that quantify the degree of precarity across three distinct dimensions: income inadequacy (mu_fm), employment insecurity (mu_fs1), and the lack of rights and protections (mu_fs2). These component measures are then synthesized into a final composite indicator, the Fuzzy Precarious Employment Index (fpei), structuring the dataset for quantitative cross-sectional and longitudinal analysis of employment conditions at national, regional (region), and state (state) levels
Evaluation of the protein content of small extracellular vesicles derived from human melanoma cells treated with Doxorubicin
No full textilustraciones, diagramasLas Vesículas Extracelulares (EV) son estructuras membranosas liberadas al espacio extracelular, que transportan biomoléculas y permiten la modulación de microambientes locales y distantes. En particular las células cancerígenas liberan EV, incluyendo vesículas extracelulares pequeñas (sEV) como exosomas, que participan en la comunicación intercelular. Estudios in vitro han mostrado que el tratamiento con Doxorrubicina (Doxo) incrementa el nivel de secreción de sEV. Este agente quimioterapéutico puede inducir respuestas de estrés celular que alteran el microambiente tumoral y, además, se ha asociado con efectos secundarios como la cardiotoxicidad.
Este estudio evaluó el efecto de Doxo (10 nM) sobre la biogénesis, producción y contenido de sEV derivadas de células de melanoma humano A375, realizando un análisis multidimensional. A nivel intracelular, la inmunocitoquímica mostró un leve aumento en los marcadores característicos de sEV, destacándose especialmente CD81 a las 96 horas (p = 0,0083). Asimismo, se observó un incremento en las estructuras positivas relacionadas con como cuerpos multivesiculares y exosomas a las tinciones con naranja de acridina (A.O) y Bodipy-TR en células vivas a las 24 y 96 horas, respectivamente. Las sEV fueron aisladas del medio tras 48 horas de condicionamiento, utilizando cromatografía de exclusión por tamaño (SEC). El análisis por seguimiento de nanopartículas evidenció un aumento significativo en la concentración de sEV (13,6 veces; p = 0,000014), acompañado de cambios en la distribución de tamaños en la población tratada (Doxo: 135,4± 5,60 nm). Mientras, la microscopía electrónica de barrido (SEM) mostró morfologías compatibles con un proceso de vesiculación activa. Por otro lado, la detección de citoquinas mediante un arreglo de anticuerpos indicó un aumento en los niveles de KITLG, CXCL1, CXCL12, CCL5, VEGF, IL-3, IL-4 e IL-10, siendo TGF-β la más destacada (p = 0,0134). El análisis bioinformático sugirió que algunas de las citoquinas encontradas en estas sEV podrían estar implicadas en la progresión tumoral y en efectos cardiotóxicos inducidos por el tratamiento.
En conjunto, los resultados evidenciaron que la exposición de células A375 a Doxo 10nM potencian la biogénesis y liberación de sEV, sugiriendo que la diseminación de sEV con elevado contenido de TGF-β puede contribuir a los efectos a nivel paracrino y autocrino en su microambiente, y a efectos secundarios atribuidos a la Doxo (Texto tomado de la fuente).Extracellular Vesicles (EV) are membrane-bound structures released into the extracellular space that transport biomolecules and enable the modulation of local and distant microenvironments. Cancer cells actively secrete EV, including small extracellular vesicles (sEV) such as exosomes, which play a key role in intercellular communication. In vitro, studies have demonstrated that treatment with Doxorubicin (Doxo) leads to an increased secretion of sEV. This chemotherapeutic agent can induce cellular stress responses that alter the tumor microenvironment and has also been associated with side effects such as cardiotoxicity.
This study evaluated the effect of Doxo (10 nM) on biogenesis, production, and content of sEV derived from human melanoma A375 cells through multidimensional analysis. At the intracellular level, immunocytochemistry revealed a slight increase in characteristic sEV markers, with a notable rise in CD81 expression at 96 hours (p = 0.0083). Furthermore, an increased presence of structures associated with multivesicular bodies and exosomes was detected through acridine orange (A.O.) and Bodipy-TR staining in live cells at 24 and 96 hours, respectively. sEV were isolated from the conditioned medium after 48 hours using size-exclusion chromatography (SEC). Nanoparticle tracking analysis revealed a significant increase in sEV concentration (13.6 F.C; p = 0.000014), accompanied by changes in size distribution within the treated population (Doxo: 135.4 ± 5.60 nm). Meanwhile, scanning electron microscopy (SEM) showed morphologies consistent with active vesiculation. On the other hand, cytokine detection using an antibody array indicated increased levels of KITLG, CXCL1, CXCL12, CCL5, VEGF, IL-3, IL-4, and IL-10, with TGF-β being the most prominent (p = 0.0134). Bioinformatic analysis suggested that some of the cytokines in these sEV might be involved in tumor progression and Doxo-induced cardiotoxic effects.
Altogether, the results demonstrated that exposure of A375 cells to 10 nM Doxo enhances the biogenesis and release of sEV, suggesting that the dissemination of sEV with high TGF-β content may contribute to paracrine and autocrine effects within their microenvironment, as well as to side effects attributed to Doxo.MaestríaMagíster en Ciencias – BioquímicaBases moleculares de la diferenciación celular y transformación malign
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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